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| 1 | Sorafenib inhibits growth and metastasis of hepatocellular carcinoma by blocking STAT3显示文摘AIM: To investigate the inhibitory role and the underlying mechanisms of sorafenib on signal transducer and activator of transcription 3 (STAT3) activity in hepatocellular carcinoma (HCC).METHODS: Human and rat HCC cell lines were treated with sorafenib. Proliferation and STAT3 dephosphorylation were assessed. Potential molecular mechanisms of STAT3 pathway inhibition by sorafenib were evaluated. In vivo antitumor action and STAT3 inhibition were investigated in an immunocompetent orthotopic rat HCC model.RESULTS: Sorafenib decreased STAT3 phosphorylationat the tyrosine and serine residues (Y705 and S727), but did not affect Janus kinase 2 (JAK2) and phosphatase shatterproof 2 (SHP2), which is associated with growth inhibition in HCC cells. Dephosphorylation of S727 was associated with attenuated extracellular signal-regulated kinase (ERK) phosphorylation, similar to the effects of a mitogen-activated protein kinase (MEK) inhibitor U0126, suggesting that sorafenib induced S727 dephosphorylation by inhibiting MEK/ERK signaling. Meanwhile, sorafenib could also inhibit Akt phosphorylation, and both the phosphatidylinositol-3-kinase (PI3K) inhibitor LY294002 and Akt knockdown resulted in Y705 dephosphorylation, indicating that Y705 dephosphorylation by sorafenib was mediated by inhibiting the PI3K/Akt pathway. Finally, in the rat HCC model, sorafenib signifi cantly inhibited STAT3 activity, reducing tumor growth and metastasis.CONCLUSION: Sorafenib inhibits growth and metastasis of HCC in part by blocking the MEK/ERK/STAT3 and PI3K/Akt/STAT3 signaling pathways, but independent of JAK2 and SHP2 activation. | Fang-Ming Gu, Quan-Lin Li, Qiang Gao, Jia-Hao Jiang, Xiao-Yong Huang, Jin-Feng Pan, Jia Fan, Jian ZhouFang-Ming Gu, Quan-Lin Li, Qiang Gao, Jia-Hao Jiang, Xiao-Yong Huang, Jin-Feng Pan, Jia Fan, Jian Zhou, Liver Cancer Institute, Zhongshan Hospital and Shanghai Medical School, Fudan University, Shanghai 200032, China Author contributions: Gu FM, Li QL and Gao Q contributed equally to this work Gu FM and Li QL performed the experi- ments and interpretation of the data and statistical analysis Zhou J and Gao Q contributed to the conception and design of the study Gu FM, Gao Q, Li QL and Zhou J wrote the manuscript Jiang JH, Huang XY, Pan JF and Fan J made substantial contri- bution to the design and conception of the study and interpreta- tion of data all authors read and approved the f inal manuscript. | 2011 | World Journal of Gastroenterology2011，17，34： | 18 |
| 2 | Portal vein embolization by fine needle ethanol injection :experimental and clinicalstudies显示文摘AIM To improve the technique of intraportal embolization (PVE) therapy, a new embolic method, was devised and the safety, effectiveness and feasibility were evaluated. METHODS PVE with intraportal ethanol injection via a fine needle was performed in 28 normal dogs, 22 SD rats, and 24 cirrhotic SD rats. After PVE, portography, histological and functional alteration of the liver were evaluated in dogs and rats, and the changes in portal hemodynamics as well as hepatic anatomy were observed in rats. In the clinical study, PVE by ethanol injection was performed in 61 patients with hepatocellular carcinoma under the guidance of portoechography with intraportal injection of CO 2. The effect of PVE was evaluated by ultrasonography and laparotomy. RESULTS The effectiveness and toxicity were dependent on the dose of ethanol. In the dogs, 0 25*!mg/*!kg of ethanol caused incomplete embolization with least liver damage, while 1 0*!mg/*!kg induced complete embolization with a high mortality of 57 1% (4/*!7) due to respiratory arrest. The dose of 0 5*!mg/*!kg resulted in complete embolization with slight toxicity to the liver. In the rats, the survival rate was 100% in normal group but 40 9% in cirrhotic models after ethanol injection by dose of 0 05*!mg/*!100*!g . PVE for cirrhotic rats with 0 03*!mg/*!100*!g of ethanol induced satisfactory embolization with significant hypertrophy in nonembolized lobes, and only slight damage to the hepatic parenchyma, and transient alteration in liver function, portal pressure and portal flow. In the clinical study, 12 cases with reverse portal flow were excluded judged by portoechography. Satisfactory embolization was gained in 90 2% (55/*!61) of the remaining patients determined by ultrasonography and surgery. All cases ran an uneventful postembolization course with no aberrant embolization. CONCLUSION PVE with intraportal ethanol injection of appropriate dosage via a fine needle is safe and effective and has several advantages comparing with transcatheter method. Portoechography is a mandatory approach for the prevention of aberrant embolization. | Lu MD Chen JW Xie XY Liang LJ Huang JF | 1999 | World Journal of Gastroenterology1999，5，6： | 15 |
| 3 | Over-expression of heat shock protein 27 attenuates doxorubicin-induced cardiac dysfunction in mice显示文摘 | Liu,L Zhang,X Qian,B Min,XY Gao,X Li,CF Cheng,YL Huang,J | 2007 | 南京医科大学学报(自然科学版)2007，27，11： | 15 |
| 4 | Laparoscopic hepatectomy by curettage and aspiration:a new technique显示文摘背景 Laparoscopic 外科为最小的侵略和快速的手术后的恢复是有益的。自从在在 1990 年代的肝切除术的 laparoscopic hepatectomy 的使用,它在有效、安全的技术的一些机构 worldwide.Lack 被执行了因为肝办理是在 1998 由刮术和渴望为 laparoscopic hepatectomy 阻止一种新技术它开发的进一步的 development.We 的主要障碍。在这份报纸,我们总共在 7 年 practice.Methods 以后分析这种技术的临床的结果 59 个连续病人在我们的机构从 1998 年 8 月由刮术和渴望经历了 laparoscopic hepatectomy 到 2005 年 1 月。这些病人包括了 33 男性和 26 女性,与 47 年的吝啬的年龄。为肝 transecsion laparoscopic, Peng 的多功能的起作用的析象器(LPMOD ) 被使用。损害在 5 .Procedures 在 19 个病人,在 17 的良性的肝肿瘤,在 18 的 intrahepatic 演算,和另外的肝损害包括了恶意的肝肿瘤在 30 个病人,的包括的本地切除术离开了在 28 的侧面的 segmentectomy,和权利在 1 个 .Results Laparoscopic 手术的 hemihepatectomy 在 57 个病人被完成。二个病人(3.4%) 把操作变换成剖腹术。吝啬的操作时间是 143 分钟,吝啬的 intraoperative 血损失是 456 mi。手术后的医院停留的吝啬的长度是 7 天。复杂并发症在 2 个病人(3.4%) 发生了,并且由刮术没有 perioperative death.Conclusion Laparoscopic hepatectomy,渴望为肝切除术有效、安全。 | CAI Xiu-jun LIANG Xiao WANG Yi-fan YU Hong ZHENG Xue-yong HUANG Di-yu PENG Shu-you Department of General Surgery,Sir Run Run Shaw Hospital,College of Medicine Institute of Minimally Invasive Surgery of Zhejiang University,Hangzhou 310016,China (Cai XJ,Liang X,Wang YF,Yu H,Zheng XY,Huang DY and Peng SY) | 2007 | Chinese Medical Journal2007，，20： | 11 |
| 5 | 血清ALT水平影响非创伤性常规指数诊断乙型肝炎相关肝硬化的准确性显示文摘【据Joural of Viral Hepatitis 2020年8月报道】题:血清ALT水平影响非创伤性常规指数诊断乙型肝炎相关肝硬化的准确性(作者Chen YP等)研究表明,FIB-4及APRI等常规指数诊断慢性乙型肝炎肝硬化、显著肝纤维化在不同研究中存在差异。近期,研究者们也先后提出了多项新的常规指数,包括红细胞体积分布宽度-血小板比值(RPR)、谷氨酰转肽酶-血小板比值(GPR)及简易肝纤维化指数(eLIFT)。为评估ALT水平对这些常规指数诊断表现的影响,1005例慢性乙型肝炎患者被纳入回顾性分析,这些患者均已接受肝活检及常规血液检查。在该研究中,似然比10、0.1的对应值分别被确定为确认诊断、排除诊断界值。 | 林小钰 陈永鹏 CHEN YP HUANG LW LIN XY | 2020 | 临床肝胆病杂志2020，36，9： | 7 |
| 6 | Antibody detection and sequence analysis of sporadic HEV in Xiamen region显示文摘 | Huang RT Li XY Xia XB Yuan XT Liu MX Li DR | 1999 | World Journal of Gastroenterology1999，5，3： | 6 |
| 7 | 在体背根神经节钙成像方法解析内脏痛及牵涉痛的新机制显示文摘内脏痛是指来自机体内在器官病理改变或功能异常引起的疼痛,常与多种内脏疾病相关,如急慢性肠炎等。内脏痛往往伴有躯体一定部位的牵涉痛。到目前为止,内脏痛及其牵涉痛的机制仍不清楚。该研究采用在体背根神经节钙成像方法(in vivo DRG calcium imaging),大视野实时动态观察和研究内脏痛及牵涉痛的背根节(dorsal root ganglion,DRG)神经元相关机制。 | 刘风雨(译) Gao XY Han S Huang Q | 2021 | 中国疼痛医学杂志2021，27，10： | 5 |
| 8 | Herbal compound 'Songyou Yin' reinforced the ability of interferon-alfa to inhibit the enhanced metastatic potential induced by palliative resection of hepatocellular carcinoma in nude mice显示文摘 | Huang XY Huang ZL Wang L | | 0，，： | 1 |
| 9 | Color Doppler velocity profile assessment of portal hemodynamics in cirrhotic patients with portal hypertension:correlation with esophageal variceal bleeding显示文摘 | Yin XY Lu MD Huang JF | | 0，，01： | 1 |
| 10 | Apelin modulates aor- tic vascular tone via endothelial nitric oxide synthase phosphorylation pathway in diabetic mice显示文摘 | Zhong JC Yu XY Huang Y | 2007 | Cardiovasc Res2007，74，3： | 1 |
| 11 | Disease burden of chronic ob-structive pulmonary diseases in west rural areas of China,2004-2005显示文摘 | Shi XQ Hu N Li XY Huang ZJ | 2011 | Zhonghua Yu Fang Yi Xue Za Zhi2011，45，1： | 1 |
| 12 | dendritic cells genetically engineered to express Fas ligand induce donor-specific hyporesponsiveness and prolong allograft survival显示文摘 | Min WP Gorczynski R Huang XY | 2000 | J Immunol2000，164，1： | 1 |
| 13 | Beneficial effect of trlpterine on systemic lupus cry - thematosus induced by active chromatin in BALB/c mice 显示文摘 | JC Ho G Tipoe L Zhang XY Huang | 2005 | Eur J Pharmacol2005，512，23： | 1 |
| 14 | Metagenomic analysis of fe ver, thrombocytopenia and leukopenia syndrome (FTLS) in Henan Province, China: discovery of a new bunyavirus显示文摘 | Xu BL Liu LC Huang XY | 2011 | PLoS Pathog2011，7，10： | 1 |
| 15 | Therapeutically relevant concentrations of raloxifene dilate pressurized rat resistance arteries via calcium-dependent endothelial nitric oxide synthase activation显示文摘 | Chan YC Leung FP Wong WT Tian XY Yung LM Lau CW Tsang SY Yao X Chen ZY Huang Y | | 0，，： | 1 |
| 16 | Metagenomic analysis of fever, thromborytopenia and leukopenia syndrome (FTLS) in Henan Province,China:discovery of a new bunyavirus显示文摘 | Xu BL Liu Lc Huang XY | 2011 | PloS Pathog2011，7，10： | 1 |
| 17 | Color Doppler velocity pmfile assessment of portal hemodymamics in cirrhotic patients with portal hypertension : Correlation with esophageal variceal bleeding显示文摘 | Yin XY Lu MD Huang JF | 2001 | J Clin Ultrasound2001，29，1： | 1 |
| 18 | Anti - tumor effect of pEgr- IFNgamma gene - radiotherapy in B16 melanoma -bearing mice显示文摘 | Wu CM Li XY Huang TH | 2004 | World J Gastroenterol2004，10，20： | 1 |
| 19 | Fatal liver failure with the emergence of hepatitis B surface antigen variants with multiple stop mutations after discontinuation of lamivudine therapy 显示文摘 | Zhang JM Wang XY Huang YX | 2006 | J Med Virol2006，78，3： | 1 |
| 20 | Fatal liver failure with the emergence of hepatitis B surface antigen variants with multiple stop mutations after discontinuation of lamivudine therapy显示文摘 | Zhang JM Wang XY Huang YX | 2006 | J Med Virol2006，78，3： | 1 |
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