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| 1 | Autologous CD34^+ and CD133^+ stem cells transplantation in patients with end stage liver disease显示文摘AIM:To assess the utility of an autologous CD34 + and CD133 + stem cells infusion as a possible therapeutic modality in patients with end-stage liver diseases.METHODS:One hundred and forty patients with endstage liver diseases were randomized into two groups.Group 1,comprising 90 patients,received granulocyte colony stimulating factor for five days followed by autologous CD34 + and CD133 + stem cell infusion in the portal vein.Group 2,comprising 50 patients,received regular liver treatment only and served as a control group.RESULTS:Near normalization of liver enzymes and improvement in synthetic function were observed in 54.5% of the group 1 patients;13.6% of the patients showed stable states in the infused group.None of the patients in the control group showed improvement.No adverse effects were noted.CONCLUSION:Our data showed that a CD34 + and CD133 + stem cells infusion can be used as supportive treatment for end-stage liver disease with satisfactory tolerability. | Hosny Salama Abdel-Rahman N Zekri Abeer A Bahnassy Eman Medhat Hanan A Halim Ola S Ahmed Ghada Mohamed Sheren A Al Alim Ghada M Sherif | 2010 | World Journal of Gastroenterology2010，16，42： | 16 |
| 2 | Pivotal role of long non-coding ribonucleic acid-X-inactive specific transcript in regulating immune checkpoint programmed death ligand 1 through a shared pathway between miR-194-5p and miR-155-5p in hepatocellular carcinoma显示文摘BACKGROUND Anti-programmed death therapy has thrust immunotherapy into the spotlight.However,such therapy has a modest response in hepatocellular carcinoma(HCC).Epigenetic immunomodulation is a suggestive combinatorial therapy with immune checkpoint blockade.Non-coding ribonucleic acid(ncRNA)driven regulation is a major mechanism of epigenetic modulation.Given the wide range of ncRNAs that co-opt in programmed cell-death protein 1(PD-1)/programmed death ligand 1(PD-L1)regulation,and based on the literature,we hypothesized that miR-155-5p,miR-194-5p and long non-coding RNAs(lncRNAs)X-inactive specific transcript(XIST)and MALAT-1 are involved in a regulatory upstream pathway for PD-1/PD-L1.Recently,nutraceutical therapeutics in cancers have received increasing attention.Thus,it is interesting to study the impact of oleuropein on the respective study key players.AIM To explore potential upstream regulatory ncRNAs for the immune checkpoint PD-1/PD-L1.METHODS Bioinformatics tools including microrna.org and lnCeDB software were adopted to detect targeting of miR-155-5p,miR-194-5p and lncRNAs XIST and MALAT-1 to PD-L1 mRNA,respectively.In addition,Diana tool was used to predict targeting of both aforementioned miRNAs to lncRNAs XIST and MALAT-1.HCC and normal tissue samples were collected for scanning of PD-L1,XIST and MALAT-1 expression.To study the interaction among miR-155-5p,miR-194-5p,lncRNAs XIST and MALAT-1,as well as PD-L1 mRNA,a series of transfections of the Huh-7 cell line was carried out.RESULTS Bioinformatics software predicted that miR-155-5p and miR-194-5p can target PDL1,MALAT-1 and XIST.MALAT-1 and XIST were predicted to target PD-L1 mRNA.PD-L1 and XIST were significantly upregulated in 23 HCC biopsies compared to healthy controls;however,MALAT-1 was barely detected.MiR-194 induced expression elevated the expression of PD-L1,XIST and MALAT-1.However,overexpression of miR-155-5p induced the upregulation of PD-L1 and XIST,while it had a negative impact on MALAT-1 expression.Knockdown of XIST did have an impact on PD-L1 expression;however,following knockdown of the negative regulator of X-inactive specific transcript(TSIX),PD-L1 expression was elevated,and abolished MALAT-1 activity.Upon co-transfection of miR-194-5p with siMALAT-1,PD-L1 expression was elevated.Co-transfection of miR-194-5p with siXIST did not have an impact on PD-L1 expression.Upon co-transfection of miR-194 with siTSIX,PD-L1 expression was upregulated.Interestingly,the same PD-L1 expression pattern was observed following miR-155-5p cotransfections.Oleuropein treatment of Huh-7 cells reduced the expression profile of PD-L1,XIST,and miR-155-5p,upregulated the expression of miR-194-5p and had no significant impact on the MALAT-1 expression profile.CONCLUSION This study reported a novel finding revealing that opposing acting miRNAs in HCC,have the same impact on PD-1/PD-L1 immune checkpoint by sharing a common signaling pathway. | Sara M Atwa Heba Handoussa Karim M Hosny Margarete Odenthal Hend M El Tayebi | 2020 | World Journal of Hepatology2020，12，12： | 9 |
| 3 | Role of relevant immune-modulators and cytokines in hepatocellular carcinoma and premalignant hepatic lesions显示文摘AIM To assess the levels of different immune modulators in patients with hepatocellular carcinoma(HCC),in relation to other hepatic diseases.METHODS Eighty-eight patients were included in the current study and represented patients with HCC(20),liver cirrhosis(28) and chronic hepatitis(CH;25),and normal controls(NC;15).Peripheral blood was isolated for immunophenotyping of active myeloid dendritic cells(m DCs;CD1 c and CD40),mature inactive myeloid cells(CD1 c and HLA),active plasmacytoid cells(p DCs;CD303 and CD40),mature inactive p DCs(CD30 and HLA),active natural killer(NK) cells(CD56 and CD161),active NK cells(CD56 and CD314) and inactive NK cells(CD56 and CD158) was done by flow cytometry.Serum levels of interleukin(IL)-2,IL-10,IL-12,IL-1β,interferon(IFN)-α,IFN-γ and tumor necrosis factor(TNF)-αR2 were assessed by ELISA.RESULTS Active m DCs(CD1 C+/CD40+) and inactive m DCs(CD1 c+/HLA+) were significantly decreased in HCC patients in relation to NC(P < 0.001).CD40+ expression on active p DCs was decreased in HCC patients(P < 0.001),and its level was not significantly changed among other groups.Inactive p DCs(CD303+/HLA+),inactive NKs(CD56+/CD158+) and active NKs(CD56+/CD161+) were not statistically changed among the four groups studied;however,the latter was increased in CH(P < 0.05).NKG2 D was statistically decreased in HCC,CH and cirrhosis(P < 0.001),and it was not expressed in 63%(12/20) of HCC patients.There was significant decrease of IL-2,IFN-α and IFN-γ(P < 0.001),and a significant increase in IL-10,IL-1β,and TNF-αR2(P <0.01,P < 0.001 and P < 0.001;respectively) in HCC patients.There was inverted correlation between IL-12 and IL-1β in HCC(r =-0.565,P < 0.01),with a strong correlation between p DCs(CD303+/CD40+) and NKs(CD56+/CD161+;r = 0.512,P < 0.05) as well as inactive m DCs(CD1 c+/HLA+) and inactive NK cells(CD56+/CD158+;r = 0.945,P < 0.001).CONCLUSION NKG2 D,CD40,IL-2 and IL-10 are important modulators in the development and progression of HCC. | Abdel-Rahman N Zekri Somaya El Deeb Abeer A Bahnassy Abeer M Badr Mona S Abdellateif Gamal Esmat Hosny Salama Marwa Mohanad Ahmed Esam El-dien Shimaa Rabah Assmaa Abd Elkader | 2018 | World Journal of Gastroenterology2018，24，11： | 6 |
| 4 | Expression of cell cycle-associated proteins in nonmuscle-invasive bladder cancer:correlation with intravesical recurrence following transurethral resection显示文摘 | Hosny MB Hideaki M Medhat AA | | 0，，： | 1 |
| 5 | Application of particle image ve- locimetry for the measurement of the airflow characteristics in an aircraft cabin 显示文摘 | Mo H Hosni M Jones B | 2003 | ASHRAE Transactions2003，109，2： | 1 |
| 6 | A new type of synthesis oil, 2,3-thiadiazole and 1,2,3-diazaphosphole derivatives via Hurd-Mori cyclization 显示文摘 | Hosny M A El-sayed T H El-sawi E A | 2012 | E-Journal of Chemistry2012，9，3： | 1 |
| 7 | Increasing antigenecity of B16 melanoma cell fraction with microware nyperthemia 显示文摘 | Santini R Hosni M Douse T | 1986 | J Microwave power1986，21，： | 1 |
| 8 | Novel isoflavone, cinnamic acid, and triterpenoid glycosides in soybean molasses 显示文摘 | HOSNY M ROSAZZ P N | 1999 | J Nat Prod1999，62，6： | 1 |
| 9 | Six cases of fibromatosis of the head and neck in children显示文摘 | TOSTEVIN P M WYATT M HOSNI A | 2000 | Int J Pediatr Otorhinolaryngol2000，53，： | 1 |
| 10 | Managing flexion knee deformity using a circular frame显示文摘 | Hosny GA Fadel M | | 0，，12： | 1 |
| 11 | Relationship between anterior chamber depth, refractive state, corneal diameter, and axial length显示文摘 | Hosny M Alio JL Claramonte P | 2000 | J Refract Surg2000，16，3： | 1 |
| 12 | Novel bis(1 acyl 2- pyrazolines) of potential anti inflammatory and molluscicidal properties显示文摘 | Barsoum F F Hosni H M Girgis A S | 2006 | Bioorganic & Medicinal Chemistry2006，14，11： | 1 |
| 13 | Hypoglycemic effect of oral insulin in diabetic rabbits using pH-dependent coated capsules containing sodium salicylate without and with sodium cholate 显示文摘 | HOSNY E A GHILZAI N M ALONAJAR T A | 1998 | Drug Dev Ind Pharm1998，24，3： | 1 |
| 14 | Oral delivery of insulin from enteric-coated capsules containing sodium salicylate: effect on rela- tive hypoglycemia of diabetic beagle dog 显示文摘 | Hosny E A AL2Shora H I Elmazar M A | 2002 | Int J Pharm2002，237，12： | 1 |
| 15 | Bifurcation Analysis of Surge and Rotating Stall in Axial Flow Compressors显示文摘 | Abed E H Houpt P K Hosny W M | 1993 | Journal of Turbomachinery1993，115，： | 1 |
| 16 | Effective intestinal absorption of insulin in diabetic rats using enteric coated capsules containing sodium salicylate 显示文摘 | Hosny E A Ghilzai N M K Al-Dhawalie A H | 1995 | Drug Dev Ind Pharm1995，21，： | 1 |
| 17 | Novel isoflavone, cinnamic acid and triterpenoid glycosides in soyben molasses显示文摘 | Hosny M H Rosazza P N | 1999 | J Nat Prod1999，62，： | 1 |
| 18 | Metal chelates with some cellulose derivatives (Ⅲ): Synthesis and structural chemistry of nickel (Ⅱ) and copper (Ⅱ) complexes with carboxymethyl cellulose 显示文摘 | Hosny W M Abdel Hadi A K EI-Saied H Basta A H | 1995 | Polymer International1995，37，： | 1 |
| 19 | Phenylpropanoid glycosides from Marrubium alysson 显示文摘 | CALIS I HOSNY M KHALIFA T | 1992 | Phytochemlstry1992，31，10： | 1 |
| 20 | Relationship between anterior chamber depth, refractive state, corneal diameter, and axial length 显示文摘 | Hosny M Alio JL Claramonte P | 2000 | J Refract Surg2000，16，： | 1 |
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