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| 1 | Comprehensive and innovative techniques for livertransplantation in rats: A surgical guide显示文摘AIM: To investigate our learning curves of orthotopic liver transplantation (OLT) in rats and the most important factor for successful surgery. METHODS: We describe the surgical procedures for our rat OLT model, and determined the operator learning curves. The various factors that contributed to successful surgery were determined. The most important surgical factors were evaluated between successful and unsuccessful surgeries.RESULTS: Learning curve data indicated that 50 cases were required for operator training to start a study. Operative time, blood loss, warm ischemic time, anhepatic phase, unstable systemic hemodynamic state, and body temperature after surgery significantly affected surgery success by univariate analysis, while the anhepatic phase was the most critical factor for success by multivariate analysis. CONCLUSION: OLT in rats is the only liver transplantation model that provides clinically relevant and reliable results. Shortened anhepatic phase is key to success in this model. | Tomohide Hori Justin H Nguyen Yasuhiro Ogura Toshiyuki Hata Shintaro Yagi Ann-Marie T Baine Norifumi Ohashi Christopher B Eckman Aimee R Herdt Hiroto Egawa Yasutsugu Takada Fumitaka Oike Seisuke Saka-moto Mureo Kasahara Kohei Ogawa Koichiro Hata Taku Iida Yukihide Yonekawa Lena Sibulesky Kagemasa Kuribayashi Takuma Kato Kanako Saito Mie Torii Naruhiko Sahara Naoko Kamo Tomoko Sahara Motohiko Yasutomi Shinji Uemoto | 2010 | World Journal of Gastroenterology2010，16，25： | 14 |
| 2 | Oxidative stress and extracellular matrices after hepatectomy and liver transplantation in rats显示文摘AIM:To investigate oxidative stress(OS)-mediated damage and the behavior of extracellular matrices in various rat models because shear stress with portal hypertension and cold ischemia/warm reperfusion injury trigger the liver regeneration cascade after surgery. These injuries also cause fatal liver damage.METHODS: Rats were divided into four groups according to the surgery performed: control; hepatectomy with 40% liver remnant(60% hepatectomy); orthotopic liver transplantation(OLT) with whole liver graft(100% OLT); and split OLT(SOLT) with 40% graft(40% SOLT). Survival was evaluated. Blood and liver samples were collected at 6 h after surgery. Biochemical and histopathological examinations were performed. OSinduced damage, 4-hydroxynonenal, ataxia-telangiectasia mutated kinase, histone H2AX, phosphatidylinositol 3-kinase(PI3K) and Akt were evaluated by western blotting. Behavior of extracellular matrices, matrix metalloproteinase(MMP)-9, MMP-2, tissue inhibitor of metalloproteinase(TIMP)-1 and TIMP-2 were also evaluated by western blotting and zymography. RESULTS: Although 100% OLT survived, 60% hepatectomy and 40% SOLT showed poor survival. Histopathological, immunohistological, biochemical and protein assays revealed that 60% hepatectomy, 100% OLT and 40% SOLT showed liver damage. PI3K and Akt were decreased in 60% hepatectomy and 40% SOLT. For protein expression, 40% SOLT showed differences in MMP-9, MMP-2 and TIMP-2. TIMP-1 showed differences in 60% hepatectomy and 40% SOLT. For protein activity, MMP-9 demonstrated significant differences in 60% hepatectomy, 100% OLT and 40% SOLT. CONCLUSION: Under conditions with an insufficient liver remnant, prevention of OS-induced damage via the Akt/PI3K pathway may be key to improve the postoperative course. MMP-9 may be also a therapeutic target after surgery. | Tomohide Hori Shinji Uemoto Feng Chen Lindsay B Gardner Ann-Marie T Baine Toshiyuki Hata Takayuki Kogure Justin H Nguyen | 2014 | World Journal of Hepatology2014，6，2： | 7 |
| 3 | 脑缺血后N-myc下游调控基因2保护血脑屏障完整性显示文摘脑缺血后血脑屏障(BBB)的破坏与外周细胞向脑内浸润、病孔损形成与进展、以及临床病程恶化密切相关。目前BBB完整性的调控机制,尤其是在永久性缺血后的调控机制尚未明确。本研究使用小鼠永久性脑缺血模型进行相关研究,结果显示,星形胶质细胞N-myc下游调控基因2(NDRG2)可能是缺血性脑卒中后BBB通透性的调控分子,该分子与分化和应激相关。免疫组化显示,在永久性大脑中动脉闭塞(MCAO)后,NDRG2在星形胶质细胞中的表达显著增加。敲除NDRG2基因,脑梗死体积增加,免疫细胞在缺血同侧大脑半球积聚增加。MCAO后,NDRG2基因敲除小鼠缺血侧皮质的缺血灶内及灶周血管周围区域内的血清蛋白(包括纤维蛋白原和免疫球蛋白)的外渗增强此之,MCAO后NDRG2基因敲除小鼠体内基质金属蛋白酶(MMPs)的表达也显著增加。在细胞培养中,NDRG2-/-星形胶质细胞中MMP-3的表达和分泌增加,这种增加可被腺病毒介导的NDRG2再表达所逆转。综上所述,脑缺血后,星形胶质细胞内的NDRG2可能通过调节MMP的表达,而在BBB通透性的调节和免疫细胞浸润中起到重要作用。 | Takarada-Iemata M Yoshikawa A Ta HM Okitani N Nishiuchi T Aida Y Kamide T Hattori T Ishii H Tamatani T Le TM Roboon J Kitao Y Matsuyama T Nakada M Hori O 聂昊 | 2018 | 神经损伤与功能重建2018，13，4： | 4 |
| 4 | Simple and reproducible hepatectomy in the mouse using the clip technique显示文摘AIM: To investigate the reliability of massive hepatectomy models by using clip techniques. METHODS: We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction > 70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves, and histopathological findings.RESULTS: According to anatomical results, models with 75%, 80%, and 90% hepatectomy produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques. CONCLUSION: Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data. | Tomohide Hori Norifumi Ohashi Feng Chen Ann-Marie T Baine Lindsay B Gardner Toshiyuki Hata Shinji Uemoto Justin H Nguyen | 2012 | World Journal of Gastroenterology2012，18，22： | 4 |
| 5 | Matrix metalloproteinase-9 contributes to parenchymal hemorrhage and necrosis in the remnant liver after extended hepatectomy in mice显示文摘AIM:To investigate the effect of matrix metalloproteinase-9(MMP-9) on the remnant liver after massive hepatectomy in the mouse.METHODS:Age-matched,C57BL/6 wild-type(WT),MMP-9(-/-),and tissue inhibitors of metalloproteinases(TIMP)-1(-/-) mice were used.The mice received 80%-partial hepatectomy(PH).Samples were obtained at 6 h after 80%-PH,and we used histology,immunohistochemical staining,western blotting analysis and zymography to investigate the effect of PH on MMP-9.The role of MMP-9 after PH was investigated using a monoclonal antibody and MMP inhibitor.RESULTS:We examined the remnant liver 6 h after 80%-PH and found that MMP-9 deficiency attenuated the formation of hemorrhage and necrosis.There were significantly fewer and smaller hemorrhagic and necrotic lesions in MMP-9(-/-) remnant livers compared with WT and TIMP-1(-/-) livers(P < 0.01),with no difference between WT and TIMP-1(-/-) mice.Serum alanine aminotransaminase levels were significantly lower in MMP-9(-/-) mice compared with those in TIMP-1(-/-) mice(WT:476 ± 83 IU/L,MMP-9(-/-):392 ± 30 IU/L,TIMP-1(-/-):673 ± 73 IU/L,P < 0.01).Western blotting and gelatin zymography demonstrated a lack of MMP-9 expression and activity in MMP-9(-/-) mice,which was in contrast to WT and TIMP-1(-/-) mice.No change in MMP-2 expression was observed in any of the study groups.Similar to MMP-9(-/-) mice,when WT mice were treated with MMP-9 monoclonal antibody or the synthetic inhibitor GM6001,hemorrhagic and necrotic lesions were significantly smaller and fewer than in control mice(P < 0.05).These results suggest that MMP-9 plays an important role in the development of parenchymal hemorrhage and necrosis in the small remnant liver.CONCLUSION:Successful MMP-9 inhibition attenuates the formation of hemorrhage and necrosis and might be a potential therapy to ameliorate liver injury after massive hepatectomy. | Norifumi Ohashi Tomohide Hori Florence Chen Sura Jermanus Christopher B Eckman Akimasa Nakao Shinji Uemoto Justin H Nguyen | 2012 | World Journal of Gastroenterology2012，18，19： | 3 |
| 6 | Tumor vascular permeability and the EPR effect in macromolecular therapeutics: a review显示文摘 | H Maeda J Wu T Sawa Y Matsumura K Hori | 2000 | Journal of Controlled Release2000，，1： | 3 |
| 7 | High-performance liquid chromatography with conductimetric detection of perfluorocarboxylic acids and perfluorosulfonates 显示文摘 | Hori H Hayakawa E Yamashita N | 2004 | Chemosphere2004，57，： | 1 |
| 8 | ' Deactivation of copper electrode' in electrochemical reduction of CO2 显示文摘 | Hori Y Konishi H Futamura T | 2005 | Electrochimica Acta2005，50，： | 1 |
| 9 | Insulin-mediated growth in aortic smooth muscle and the vascular renin-angiotensin system显示文摘 | Hori M T Zhu J H | 1998 | Hypertension1998，32，3： | 1 |
| 10 | Brain natriuretic peptide predicts chronic atrial fibrillation after ventricular pacing in patients with sick sinus syndrome显示文摘 | HORIE H TSUTAMOTO T MINAI K | 2000 | Jpn CIRC J2000，64，2： | 1 |
| 11 | Development of Ultra-Narrow Gap Hot-Wire TIG welding process-study of hot wire welding processes (report 20)显示文摘 | Watanabe H Hori K Nakazawa N et ol | 2001 | Japan Weld Soc2001，68，4： | 1 |
| 12 | MicroRNA - 133 regulates the expression of GLUT4 by targeting KLF15 and is involved in metabolic control in cardiac myocytes 显示文摘 | Horie T Ono K Nishi H | 2009 | Biochem Biophys Res Commun2009，389，2： | 1 |
| 13 | Overexpression of ornithine decarboxylase enhances endothelial proliferation by suppressing endostatin expression显示文摘 | Nemoto T Hori H Yoshimoto M | 2002 | Blood2002，99，4： | 1 |
| 14 | Studies of methyl 2-nitroimidazole-l-acetohydroxam-ate (KIN 804) 2: effect on certain antioxidant enzyme systems in mice bearing ehrlich ascites carcinoma显示文摘 | Abu Zeid M Hori H Nagasawa H | 2000 | Biol Pharm Bull2000，23，2： | 1 |
| 15 | Brain natriuretic peptide predicts chronic atrial fibrillation after ventricular pacing in patients with sick sinus syndrome显示文摘 | Horie H Tsutamoto T Minai K | 2000 | Jpn Circ J2000，64，12： | 1 |
| 16 | Physiological role of peroxisomal beta-oxidation in liver of fasted rats显示文摘 | Ishii H Horie S Suga T | 1980 | J Biochem1980，87，： | 1 |
| 17 | A multiple Lyapunov function approach to stabilization of fuzzy control systems 显示文摘 | TANAKA K HORI T WANG H O | 2003 | IEEE Transactions on Fuzzy Systems2003，11，4： | 1 |
| 18 | Pharmacokinetic,pharmacodynamic,and tolerability prones of the dipeptidyl peptidase-4 inhibitor linagliptin:a 4-week multicenter,randomized,double-blind,placebo-controlled phase Ⅱa study in Japanese type 2 diabetes patients显示文摘 | Horie Y Kanada S Watada H | 2011 | Clin Ther2011，33，7： | 1 |
| 19 | Treatment of intraeranial dural arteriovenous fistulas:current strategies based on location and hemodynamics,and alternative techniques of trans-catheter embolization显示文摘 | Kiyosue H Hori Y Okahara M | 2004 | Radiographics2004，24，6： | 1 |
| 20 | Determination of macrolide antibiotics in meat and fish by liquid chromatography electrospray mass spectrometry显示文摘 | Horie M Takegami H Toya K | 2003 | Anal Chim Acta2003，492，12： | 1 |
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