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| 1 | Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors显示文摘Achievement of immunocompetent and therapeutic T lymphopoiesis from pluripotent stem cells(PSCs)is a central aim in T cell regenerative medicine.To date,preferentially reconstituting T lymphopoiesis in vivo from PSCs remains a practical challenge.Here we documented that synergistic and transient expression of Runx1 and Hoxa9 restricted in the time window of endothelial-to-hematopoietic transition and hematopoietic maturation stages in a PSC differentiation scheme(iR9-PSC)in vitro induced preferential generation of engraftable hematopoietic progenitors capable of homing to thymus and developing into mature T cells in primary and secondary immunodeficient recipients.Single-cell transcriptome and functional analyses illustrated the cellular trajectory of T lineage induction from PSCs,unveiling the T-lineage specification determined at as early as hemogenic endothelial cell stage and identifying the bona fide pre-thymic progenitors.The induced T cells distributed normally in central and peripheral lymphoid organs and exhibited abundant TCRαβrepertoire.The regenerative T lymphopoiesis restored immune surveillance in immunodeficient mice.Furthermore,gene-edited iR9-PSCs produced tumor-specific T cells in vivo that effectively eradicated tumor cells.This study provides insight into universal generation of functional and therapeutic T cells from the unlimited and editable PSC source. | Rongqun Guo Fangxiao Hu Qitong Weng Cui Lv Hongling Wu Lijuan Liu Zongcheng Li Yang Zeng Zhijie Bai Mengyun Zhang Yuting Liu Xiaofei Liu Chengxiang Xia Tongjie Wang Peiqing Zhou Kaitao Wang Yong Dong Yuxuan Luo Xiangzhong Zhang Yuxian Guan Yang Geng Juan Du Yangqiu Li Yu Lan Jiekai Chen Bing Liu Jinyong Wang | 2020 | Cell Research2020，30，1： | 7 |
| 2 | Controlled growth and photoconductive properties of hexagonal SnS2 nanoflakes with mesa-shaped atomic steps显示文摘We demonstrated the controlled growth of two-dimensional (2D) hexagonal tin disulfide (SnS2) nanoflakes with stacked monolayer atomic steps. The morphology was similar to flat-topped and step-sided mesa plateaus or step pyramids. The SnS2 nanoflakes were grown on mica substrates via an atmospheric-pressure chemical vapor deposition process using tin monosulfide and sulfur powder as precursors. Atomic force microscopy (AFM), electron microscopy, and Raman characterizations were performed to investigate the structural features, and a sequential layer-wise epitaxial growth mechanism was revealed. In addition, systematic Raman characterizations were performed on individual SnS2 nanoflakes with a wide range of thicknesses (1-100 nm), indicating that the A1g peak intensity and Raman shifts were closely related to the thickness of the SnS2 nanoflakes. Moreover, photoconductive AFM was performed on the monolayer-stepped SnS2 nanoflakes, revealing that the flat surface and the edges of the SnS2 atomic steps had different electrical conductive properties and photoconductive behaviors. This is ascribed to the dangling bonds and defects at the atomic step edges, which caused a height difference of the Schottky barriers formed at the interfaces between the PtIr-coated AFM tip and the step edges or the flat surface of the SnS2 nanoflakes. The 2D SnS2 crystals with regular monolayer atomic steps and fast photoresponsivity are promising for novel applications in photodetectors and integrated optoelectronic circuits. | Yi Hu Tao Chen Xiaoqi Wang Lianbo Ma Renpeng Chen Hongfei Zhu Xin Yuan Changzeng Yan Guoyin Zhu Hongling Lv Jia Liang Zhong Jin Jie Liu | 2017 | Nano Research2017，10，4： | 5 |
| 3 | Down-regulation of inducible co-stimulator (ICOS) by intravitreal injection of small interfering RNA (siRNA) plasmid suppresses ongoing experimental autoimmune uveoretinitis in rats显示文摘 | Yongsheng Hou Lin Xing Shaoying Fu Xiaoning Zhang Jingjing Liu Hongling Liu Bingjie Lv Hao Cui | 2009 | Graefe’s Archive for Clinical and Experimental Ophthalmology2009，，6： | 1 |
| 4 | Effect of Crosslinking on Sorption, Diffusion and Pervaporation of Gasoline Components in Hydroxyethyl Cellulose Mem- branes显示文摘 | Huimin Qu Ying Kong Hongling Lv et al | 2010 | Chemical Engineering Journal2010，157，： | 1 |
| 5 | Effect of crosslinking on sorption, diffusion and pervaporation of gasolinecomponents in hydroxyethyl cellulose membranes显示文摘 | Huimin Qu Ying Kong Hongling Lv | 2010 | Chemical Engineering Journal2010，157，： | 1 |
| 6 | Pluripotent stem cell-derived CD 19-CAR iT cells effectively eradicate B-cell lymphoma in vivo显示文摘Chimeric antigen receptor T cell(CAR-T)therapy is one of the most promising approaches in cancer treatment.1 However,the limited availability of patient-derived T cells narrows its universal applicability.Thus,it is necessary to invent new methods to obtain alternative T-cell sources.Pluripotent stem cells(PSCs),which have unlimited culture potential and are amenable to gene editing,are ideal for generating induced T(iT)cells. | Cui Lv Shoubing Chen Fangxiao Hu Dehao Huang Tongjie Wang Juan Du Jinyong Wang Hongling Wu | 2021 | Cellular & Molecular Immunology2021，18，3： | 0 |
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