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| 1 | Mechanisms of regulation of PFKFB expression in pancreatic and gastric cancer cells显示文摘Enzymes 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 and-4(PFKFB-3 and PFKFB-4)play a significant role in the regulation of glycolysis in cancer cells as well as its proliferation and survival.The expression of these mRNAs is increased in malignant tumors and strongly induced in different cancer cell lines by hypoxia inducible factor(HIF)through active HIF binding sites in promoter region of PFKFB-4 and PFKFB-3 genes.Moreover,the expression and hypoxia responsibility of PFKFB-4 and PFKFB-3 was also shown for pancreatic(Panc1,PSN-1,and MIA PaCa-2)as well as gastric(MKN45 and NUGC3)cancer cells.At the same time,their basal expression level and hypoxia responsiveness vary in the different cells studied:the highest level of PFKFB-4 protein expression was found in NUGC3 gastric cancer cell line and lowest in Panc1 cells,with a stronger response to hypoxia in the pancreatic cancer cell line.Overexpression of different PFKFB in pancreatic and gastric cancer cells under hypoxic condition is correlated with enhanced expression of vascular endothelial growth factor(VEGF)and Glut1 mRNA as well as with increased level of HIF-1αprotein.Increased expression of different PFKFB genes was also demonstrated in gastric,lung,breast,and colon cancers as compared to corresponding nonmalignant tissue counterparts from the same patients,being more robust in the breast and lung tumors.Moreover,induction of PFKFB-4 mRNA expression in the breast and lung cancers is stronger than PFKFB-3mRNA.The levels of both PFKFB-4 and PFKFB-3 proteins in non-malignant gastric and colon tissues were more pronounced than in the non-malignant breast and lung tissues.It is interesting to note that Panc1and PSN-1 cells transfected with dominant/negative PFKFB-3(dnPFKFB-3)showed a lower level of endogenous PFKFB-3,PFKFB-4,and VEGF mRNA expressions as well as a decreased proliferation rate of these cells.Moreover,a similar effect had dnPFKFB-4.In conclusion,there is strong evidence that PFKFB-4 and PFKFB-3 isoenzymes are induced under hypoxia in pancreatic and other cancer cell lines,are overexpressed in gastric,colon,lung,and breast malignant tumors and undergo changes in their metabolism that contribute to the proliferation and survival of cancer cells.Thus,targeting these PFKFB may therefore present new therapeutic opportunities. | Oleksandr H Minchenko Katsuya Tsuchihara Dmytro O Minchenko Andreas Bikfalvi Hiroyasu Esumi | 2014 | World Journal of Gastroenterology2014，20，38： | 17 |
| 2 | Simplified Three - dimensional Modeling of Mixture Formation and Combustion in a DI Diesel Engine 显示文摘 | HIROYASU H NISHIDA K | 1989 | Oil & Gas Science and Technology1989，75，2： | 1 |
| 3 | Development and use of spray combustion modeling to predict diesel engine efficiency and pollutions emissions显示文摘 | Hiroyasu H Kadota T Arai M | 1983 | Bull of the JSME1983，26，： | 1 |
| 4 | Biodegradation of bisphenol A and disapperarance of its estrogenic显示文摘 | Takashi H Hiroyasu N Kotaro U | 2005 | Environmental Toxicology and Chemistry2005，24，8： | 1 |
| 5 | Development and Use of a Spray Combustion Modeling to Predict Diesel Engine Efficiency and Pollutant Emissions (Part 1:Combustion Modeling)显示文摘 | Hiroyasu H Kadota T Arai M | 1983 | Bulletin of the JSME1983，26，214： | 1 |
| 6 | Development and use of spraycombustion modeling to predict diesel engine efficiency andpollutants emissions显示文摘 | Hiroyasu H Kadota T Arai M | 1983 | Bulletin of the Japan Society ofMechanical Engineers1983，，26： | 1 |
| 7 | Induction of differentiation of myelogenous leukemia cells by humulone,a bitter in the hop显示文摘 | OSHIO H HIROYASU T MAKOTO M | 1998 | Leukemia Research1998，，22： | 1 |
| 8 | Control efficacy of cyflufenamid in the field and its fungicidal properties显示文摘 | Masahiro H Homare Y Hiroyasu H | 2006 | Pestic Sci2006，31，2： | 1 |
| 9 | Development and use of spray combustion modeling to predict diesel engine ef- ficiency and pollutions emissions显示文摘 | Hiroyasu H Kadota T Arai M | 1983 | Bulletin of the JSME1983，26，： | 1 |
| 10 | Single Droplet Combustion on the Supercritical Environment 显示文摘 | Kadoda T | 1980 | JSME1980，46，408： | 1 |
| 11 | Spray breakup mechanism from the hole- type nozzle and its applications显示文摘 | Hiroyasu H | 2000 | Atomiz Sprays2000，10，345： | 1 |
| 12 | Spray Breakup Mechanism From the Hole-Type Nozzle and Its Applications 显示文摘 | Hiroyasu H | 2000 | Atomization andSprays2000，10，： | 1 |
| 13 | Mechanical properties and fracture process of SiCw/Mg composites produced by squeeze casting and extrusion 显示文摘 | Chang S Hiroyasu H Kamio A | 1997 | Mate Trans JIM1997，38，1： | 1 |
| 14 | Increased expression of membrane type 3-matrix metalloproteinase in human atherosclerotic plaque:Role of activated acrophages and inflammatory cytokines显示文摘 | Hiroyasu U Alice H Ming L | 2002 | Circulation2002，106，： | 1 |
| 15 | Simplified Three-Dimensional Modeling of Mixture Formation and Combustion in a DI Diesel Engine显示文摘 | Hiroyasu H NishidaK | 1989 | Oil & Gas Science and Technology1989，54，2： | 1 |
| 16 | Evaporation of a single droplet atelevated pressures and temperatures 显示文摘 | Kadota T Hiroyasu H | 1976 | Bulletin of theJSME-Japan Society of Mechanical Engineers1976，19，138： | 1 |
| 17 | Evaluation of the mouse lymphoma tk assay(microwell method)as an ahemative to the in vitro choromosomal aberration test显示文摘 | Makoto H Hiroyasu S | 1999 | Mutagenesis1999，14，： | 1 |
| 18 | Spontaneous Ignition Delay of a Fuel Dropet in High Pressure High Temperature Gaseous Environments显示文摘 | Kadota T Hiroyasu H | 1976 | Bull JSME1976，19，130： | 1 |
| 19 | Droplet evaporation in apressurized and heated ambient gas 显示文摘 | Hiroyasu H Kadota T | 1974 | InternationalJournal Series B-Fluids and Thermal Engineering1974，40，12： | 1 |
| 20 | Simplified Three-Dimensional Modeling of Mixture Formation and Combustion in a DI Diesel Engine显示文摘 | Hiroyasu H NishidaK | 1989 | Oil & Gas Science and Technology1989，54，2： | 1 |
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