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| 1 | Clinical diagnostic performance of the simultaneous amplification and testing methods for detection of the Mycobacterium tuberculosis complex for smear-negative or sputum-scarce pulmonary tuberculosis in China显示文摘 | Fan Lin Zhang Qing Cheng Liping Liu Zhibing Ji Xiaobing Cui Zhenling Ju Jingliang Xiao Heping | 2014 | Chinese Medical Journal2014，，10： | 19 |
| 2 | Imaging superoxide flash and metabolism-coupled mitochondrial permeability transition in living animals显示文摘线粒体为精力新陈代谢和反应的氧种类(ROS ) 的生产是必要的。在未经触动的房间,呼吸线粒体展览自发的 “ superoxide flashes”,对短暂 mitochondrial 渗透转变(tMPT ) 结果的 quantal 生产 ROS 事件。这里,我们在表示 superoxide 生物传感器 mt-cpYFP 的生活老鼠在 mitochondrial superoxide 闪光和 tMPT 活动的 vivo 成像执行第一,并且表明他们联合到整个身体的葡萄糖新陈代谢。柔韧的 tMPT/superoxide 闪光活动发生在骨胳的肌肉和 anesthetized 的臀部的神经转基因的老鼠。在骨胳的肌肉,成像 tMPT/superoxide 闪光揭示了线粒体的迷宫的三维的网络同时地操作。tMPT/superoxide 闪光活动响应全身的葡萄糖挑战或胰岛素刺激澎湃,以一种显然调制频率的方式并且也在 tMPT 的 gating 模式包含移动。因此,在 tMPT 依赖的 mitochondrial ROS 的 vivo 成像,信号和 metabolism-tMPT-superoxide 的发现闪光联合标记为 mitochondrial 功能并且 ROS 处于健康和疾病发信号的学习的重要工艺、概念的进展。 | Huaqiang Fang Min Chen Yi Ding Wei Shang Jiejia Xu Xing Zhang Wanrui Zhang Kaitao Li Yao Xiao Feng Gao Shujiang Shang Jing-Chao Li Xiao-Li Tian Shi-Qiang Wang Jingsong Zhou Noah Weisleder Jianjie Ma Kunfu Ouyang Ju Chen Xianhua Wang Ming Zheng Wang Wang Xiuqin Zhang Heping Cheng | 2011 | Cell Research2011，21，9： | 11 |
| 3 | Hydrogen peroxide primes heart regeneration with a derepression mechanism显示文摘当成年的人的心很限制了再生潜力时,成年 zebrafish 心能充分在 20% 室的切除术以后再生。尽管以前的报告建议象 FGF 和 PDGF 那样的发展发信号小径在成年的心新生被再使用,内在的细胞内部的机制仍然保持大部分未知。这里,我们显示出那 H 2 O 2 充当新奇 epicardial 和心肌的信号在成年 zebrafish 为新生告知心。未经触动的心的实时成像揭示了高度局部性的 H 2 O 2(~ 30 μ M ) 在在切除术地点的心外膜和邻近的紧缩的心肌层的生产。减少的 H 2有 Duox 禁止者 diphenyleneiodonium (点每英寸)或 apocynin 的 O 2形成,或清除 H 2当时,由过氧化氢酶 overexpression 的 O 2显著地损害了心脏的新生外长的 H 2 O 2在心脏的新生上救了点每英寸的禁止的效果,显示那 H 2 O 2是在这个过程的一个必要、足够的信号。机械学地,提高了 H 2 O 2 使动摇氧化还原作用敏感的磷酸酶 Dusp6 并且因此增加了 Erk1/2 的 phosphorylation。当 dusp6 的转基因的 overexpression 损害了心脏的新生时, Dusp6 禁止者 BCI 完成了类似的支持 regenerative 效果。H 2 O 2 在招募起一个双作用通过二条相对独立的小径的有免疫力的房间和支持的心新生。我们结束那 H 2 潜在地从 Duox/Nox2 产生的 O 2 由解开通过包含 Dusp6 的 derepression 机制发信号的地图 kinase 在 zebrafish 支持心新生。 | Peidong Han Xiao-Hai Zhou Nannan Chang Cheng-Lu Xiao Shouyu Yah He Ren Xin-Zhuang Yang Mei-Ling Zhang Qing Wu Boyang Tang Ju-Peng Diao Xiaojun Zhu Chuanmao Zhang Chuan-Yun Li Heping Cheng Jing-Wei Xiong | 2014 | Cell Research2014，24，9： | 10 |
| 4 | DJ-1 is involved in the multidrug resistance of SGC7901 gastric cancer cells through PTEN/PI3K/Akt/Nrf2 pathway显示文摘Gastric cancer is a common malignancy worldwide.The occurrence of multidrug resistance(MDR)is the major obstacle for effective gastric cancer chemotherapy.In this study,the in-depth molec-ular mechanism of the DJ-1-induced MDR in SGC7901 gastric cancer cells was investigated.The results showed that DJ-1 expression level was higher in MDR variant SGC7901NCR cells than that in its parental SGC7901 cells.Moreover,DJ-1 overexpression conferred the MDR phenotype to SGC7901 cells,while DJ-1 knockdown in SGC7901NCR cells induced re-sensitization to adriamycin,vincristine,cisplatin,and 5-fluorouracil.These results suggested that DJ-1 mediated the develop-ment of MDR in SGC7901 gastric cancer cells.Importantly,further data revealed that the activation of PI3k/Akt and Nrf2 signaling pathway were required for the DJ-1-induced MDR phenotype in SGC7901 gastric cancer cells.Meanwhile,we found that Pl3k/Akt pathway was activated probably through DJ-1 directly binding to and negatively regulating PTEN,consequently resulting in Nrf2 phosphorylation and activation,and thereby inducing Nrf2-dependent P-glycoprotein(P-gp)and Bcl-2 expressions in the DJ-1-mediated MDR of SGC7901 gastric cancer cells.Overall,these results revealed that activating PTEN/PI3K/Akt/Nrf2 pathway and subsequently upregulating P-gp and Bcl-2 expression could be a critical mechanism by which DJ-1 mediates the development of MDR in SGC7901 gastric cancer cells.The new findings may be helpful for understanding the mechanisms of MDR in gastric cancer cells,prompting its further investigation as a molecular target to overcome MDR. | Lejia Qiu Zhaoxia Ma Xiaoran Li Yizhang Deng Guangling Duan Le Zhao Xingwang Xu Lin Xiao Haoyue Liu Zhengming Zhu Heping Chen | 2020 | Acta Biochimica et Biophysica Sinica2020，52，11： | 5 |
| 5 | A Silver-Staining cDNA-AFLP Protocol Suitable for Transcript Profiling in the Latex of Hevea brasiliensis (Para Rubber Tree)显示文摘 | Xiao Xiaohu Li Heping Tang Chaorong | 2009 | Molecular Biotechnology2009，42，1： | 1 |
| 6 | Tuberculosis complicated by diabetes mellitus at Shanghai pulmonary hospital China显示文摘 | Qing Zhang Heping Xiao Isamu Sugawara | 2009 | Jpn J Infect Dis2009，62，5： | 1 |
| 7 | Is China ready to go mainstream in molecular medicine?显示文摘 | Ruiping Xiao Heping Cheng Zhuan Zhou | 2003 | Nature2003，432，： | 1 |
| 8 | Enhanced Gi Signaling Selectively Negates β2-Adrenergic Receptor (AR)– but Not β1-AR–Mediated Positive Inotropic Effect in Myocytes From Failing Rat Hearts显示文摘 | Rui-Ping Xiao Sheng-Jun Zhang Khalid Chakir Pavel Avdonin Weizhong Zhu Richard A. Bond C. William Balke Edward G. Lakatta Heping Cheng | 2003 | Circulation: Journal of the American Heart Association2003，，13： | 1 |
| 9 | Fructooligosaccharides enhance the therapeutic effect of xiao-zhi-fang on non-alcoholic fatty liver disease via regulating intestinal flora显示文摘Background:Non-alcoholic fatty liver disease(NAFLD)is a frequently occurring clinical disease,which could be treated by traditional Chinese medicine.However,the effects and mechanisms of xiao-zhi-fang(XZF)alone or in combination with fructooligosaccharides(FOS)on NAFLD remains unclear.Methods:This study established the NAFLD mouse model by feeding with a high-fat diet and by administering with XZF and/or FOS.Moreover,the fat content in the liver tissues was evaluated by oil red O and hematoxylin and eosin staining,and the liver function indices were examined using a biochemical analyzer.Changes in the intestinal flora were monitored using 16S rRNA gene sequencing.The results of the current study showed that the alanine aminotransferase,aspartate aminotransferase,total triglyc-eride,total cholesterol levels,and the amount of lipid accumulation were markedly elevated in NAFLD mice.Results:XZF alone or in combination with FOS significantly reduced lipid accumulation in the liver and blood lipid levels in NAFLD mice.Moreover,XZF alone or in combination with FOS notably altered the intestinal flora structure in NAFLD mice.Consequently,17 operational taxonomic units were decreased and nine were increased in the XZF and/or FOS groups.Conclusions:FOS may accelerate the intestinal microecological balance in NAFLD mice after treatment with XZF by promoting intestinal flora growth,thereby enhancing the therapeutic effect of XZF on NAFLD. | Jiongshan Zhang Yue Li Gemin Xiao Yongwei Li Heping Xie Yan Chen | 2021 | Liver Research2021，5，4： | 0 |
| 10 | Isolation by phage display and characterization of a single-chain antibody specific for O^6-methyldeoxyguanosine显示文摘New approaches of making single chain Fv antibodies against O6-methyl-2’ -deoxyguanosine (O6MdG) have been demonstrated by using the phage antibody display system. Using O6MdG as an antigen, 21 positive clones were identified by ELISA from this library, one of which, designated H3, specifically binds to O6MdG with high affinity. The H3 scFv antibody has an affinity constant (Kaff) of 5.94×1011(mol/L)-1. H3 scFv has been successfully used to detect O6MdG in DNA hydrolyses from yeast or E. coli cells treated with a DNA methylating agent. To our knowledge, this is the first report of the selection of a specific scFv against DNA adducts. The results demonstrate the potential applications of the phage display technology for the detection of DNA lesions caused by mutagens and carcinogens. | DAI Heping ZHAO Rouhong Haug Brenda Sean M.Hemmingsen XIAO Wei | 2001 | Chinese Science Bulletin2001，46，12： | 0 |
| 11 | Executive summary of Chinese expert consensus for topical application of anti-microbial agents for lower respiratory tract infection in adults显示文摘In recent years,with the increased prevalence of multi-drug resistant(MDR)bacterial infection and chronic pulmonary infection,the topical application of anti-microbial agents,mainly inhaled antibiotics,has come back to clinical practice.Several formulations for nebulization and dry powder inhaler(DPI)have been approved for inhaled anti-infective therapy.Meanwhile,evidence and experience have been accumulated in the use of anti-microbial agents delivered via airway.However,the available studies in this field are heterogenous in the study population,drug delivery route and dosages.The efficacy and safety of inhaled anti-infective therapy in various types of lower respiratory tract infections(LRTIs)need to be evaluated.Moreover. | Jing Zhang Qijian Cheng Yi Huang Hong Fan Guoxiang Lai Xiangdong Mu Wei Sha Danyang She Ning Shen Xin Su Jinfu Xu Feng Ye Xinlun Tian Tiantuo Zhang Hua Zhou Youning Liu Lixian He Heping Xiao Bei He Yi Shi Xiangyan Zhang Bin Cao Jieming Qu on behalf of Pulmonary Infection Assembly of Chinese Thoracic Society | 2022 | Chinese Medical Journal2022，135，22： | 0 |
| 12 | Evolution analysis of Buddhism Caves’ cultural landscape from India to China显示文摘 | Heping Li Jing Xiao | 2015 | 西部人居环境学刊2015，30，A01： | 0 |
| 13 | Predictors for Treatment Outcomes in Patients with Multi-drug Resistant Tuberculosis — China, 2018–2020显示文摘Summary What is already known about this topic?Multi-drug resistant tuberculosis(MDR-TB)is a critical global public health problem.What is added by this report?Sputum cultures and lung images show a strong association with treatment outcomes,serving as a multi-dimensional approach to identify MDR-TB patients with poor outcomes. | Shunxian Zhang Lei Qiu Dingzhong Wu Shaoyan Zhang Chenhui Pan Cui Li Heping Xiao Fuli Huang Hua Wang Feng Jiang Huiyong Zhang Peiyong Zheng Zhenhui Lu | 2022 | China CDC weekly2022，4，41： | 0 |
| 14 | Full length cDNA cloning and expression analysis of annexinA2 gene from deer antler tissue显示文摘ANXA2(AnnexinA2), a calcium-dependent phospholipid binding protein, is involved in various Ca2+-related biological activities. In the present study, full-length cDNA of ANXA2 was isolated from the velvet antler tip tissue of sika deer(Cervus nippon hortulorum); the amino acid sequence and gene expression was analyzed by using bioinformatics and real-time reverse transcriptase polymerase chain reaction(RT-PCR) techniques. Nucleotide sequence analysis reveals that the full-length cDNA of the ANXA2 gene was 1372 bp, of which 1020 bp was in the open-reading frame(ORF) encoding 339 amino acids; its relative molecular weight was 38.3 kDa; and isoelectric point was 6.72. Sequence analysis indicates that the protein includes four conserved tandem-duplication ANX domains. The gene-accession nucleotide sequence number in GenBank is JX315571. Expression analysis by RT-PCR reveals that ANXA2 gene expression has a significant positive correlation with the antler-tissue mineralization process, indicating that this gene may play an important role in the regulation of antler-tissue mineralization. | Li Hao Xianghong Xiao Heping Li | 2014 | Journal of Forestry Research2014，25，4： | 0 |
| 15 | Preliminary research and scheme design of deep underground in situ geo-information detection experiment for Geology in Time显示文摘The deep earth,deep sea,and deep space are the main parts of the national“three deep”strategy,which is in the forefront of the strategic deployment clearly defined in China’s 14th Five-Year Plan(2021-2025)and the Long-Range Objectives Through the Year 2035.It is important to reveal the evolutionary process and mechanism of deep tectonics to understand the earth’s past,present and future.The academic con-notation of Geology in Time has been given for the first time,which refers to the multi-field evolution response process of geological bodies at different time and spatial scales caused by geological processes inside and outside the Earth.Based on the deep in situ detection space and the unique geological envi-ronment of China Jinping Underground Laboratory,the scientific issue of the correlation mechanism and law between deep internal time-varying and shallow geological response is given attention.Innovative research and frontier exploration on deep underground in situ geo-information detection experiments for Geology in Time are designed to be carried out,which will have the potential to explore the driving force of Geology in Time,reveal essential laws of deep earth science,and explore innovative technologies in deep underground engineering. | Heping Xie Ru Zhang Zetian Zhang Yinshuang Ai Jianhui Deng Yun Chen Yong Zhou Mingchuan Li Liqiang Liu Mingzhong Gao Zeqian Yang Weiqiang Ling Heng Gao Qijun Hao Kun Xiao Chendi Lou | 2024 | International Journal of Mining Science and Technology2024，34，1： | 0 |
| 16 | Dynamic mechanical characteristics of deep Jinping marble in complex stress environments显示文摘To reveal the dynamic mechanical characteristics of deep rocks,a series of impact tests under triaxial static stress states corresponding to depths of 300-2400 m were conducted.The results showed that both the strain rates and the stress environments in depth significantly affect the mechanical characteristics of rocks.The sensitivity of strain rate to the dynamic strength and deformation modulus shows a negative correlation with depth,indicating that producing penetrative cracks in deep environments is more difficult when damage occurs.The dynamic strength shows a tendency to decrease and then increase slightly,but decreases sharply finally.Transmissivity demonstrates a similar trend as that of strength,whereas reflectivity indicates the opposite trend.Furthermore,two critical depths with high dynamically induced hazard possibilities based on the China Jinping Underground Laboratory(CJPL)were proposed for deep engineering.The first critical depth is 600-900 m,beyond which the sensitivity of rock dynamic characteristics to the strain rate and restraint of circumferential stress decrease,causing instability of surrounding rocks under axial stress condition.The second one lies at 1500-1800 m,where the wave impedance and dynamic strength of deep surrounding rocks drop sharply,and the dissipation energy presents a negative value.It suggests that the dynamic instability of deep surrounding rocks can be divided into dynamic load dominant and dynamic load induced types,depending on the second critical depth. | Chendi Lou Heping Xie Ru Zhang Hai Ren Hao Luo Kun Xiao Yuan Peng Qiang Tan Li Ren | 2024 | Journal of Rock Mechanics and Geotechnical Engineering2024，16，2： | 0 |
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