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| 1 | Protective effects of anti-ricin A-chain RNA aptamer against ricin toxicity显示文摘AIM: To investigate the therapeutic potential of an RNA ligand (aptamer) specific for the catalytic ricin A-chain (RTA), the protective effects of a 31-nucleo-tide RNA aptamer (31RA), which formed a high affi nity complex with RTA, against ricin-induced toxicity in cellbased luciferase translation and cell cytotoxicity assays were evaluated. METHODS: To test the therapeutic potential of anti-RTA aptamers in Chinese hamster ovary (CHO) AA8 cells stably transfected with a tetracycline regulat-able promoter, ricin ribotoxicity was measured using luciferase and ricin-induced cytotoxicity was ascertained by MTS cell proliferation assay with tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium]. RESULTS: Inhibition of protein synthesis by ricin in CHO AA8 cells resulted in diminished luciferase activity and treatment with polyclonal antibody against degly-cosylated RTA (dgA) neutralized the inhibitory effects of ricin on luciferase activity and protected against ricin-induced cytotoxicity as measured by MTS assay. The 31RA anti-RTA aptamer inhibited the translation of luciferase mRNA in cell-free reticulocyte translation as- say. 31RA aptamer also partially neutralized the inhibitory effects of ricin on luciferase activity and partially protected against ricin-induced cytotoxicity in CHO AA8 cells. CONCLUSION: We have shown that anti-RTA RNA aptamer can protect against ricin ribotoxicity in cellbased luciferase and cell cytotoxicity assays. Hence, RNA aptamer that inhibits RTA enzymatic activity represents a novel class of nucleic acid inhibitor that has the potential to be developed as a therapeutic agent for the treatment of ricin intoxication. | Shaoan Fan Feng Wu Frank Martiniuk Martha L Hale Andrew D Ellington Kam-Meng Tchou-Wong | 2008 | World Journal of Gastroenterology2008，14，41： | 3 |
| 2 | Relationship between no reflow and infarct size as influenced by the duration of ischemia and reperfusion显示文摘 | HALE S L LI G | 2002 | Am J Physiol Heart Circ Physiol2002，282，2： | 1 |
| 3 | No-re/low phe- nomenon persists long-term after ischemiaJreperfusion in the rat and predicts infarct expansion 显示文摘 | REFFELMANN T HALE S L DOW J S | 2003 | Circulation2003，108，23： | 1 |
| 4 | Zinc alpha-2-gly- coprotein is expressed by malignant prostatic epithelium and may serve as a potential serum marker for prostate cancer 显示文摘 | Hale L P Price D T Sanchez L M | 2001 | Clin Cancer Res2001，7，4： | 1 |
| 5 | Ovarian epithelial tumors: expression of PCNA显示文摘 | Gao L Wilkinson N Hale R | 1992 | J Pathol1992，，： | 1 |
| 6 | Use of Thermal and Vegetation Index Data from Earth Observing Satellites to Evaluate the Risk of Schistosomiasis in Bahia, Brall 显示文摘 | Bavia ME Malone JB Hale L | 2001 | Acta Tropica2001，79，1： | 1 |
| 7 | Collision Induced Dissociation of Fe2^+ 显示文摘 | Loh S K Lian L Hales D A | 1988 | J Phys Chem1988，92，： | 1 |
| 8 | Optimal simultaneous structural and control design of maneuvering flexible spacecraft 显示文摘 | Hale A L Dahl W E Lisowski R J | 1984 | Journal of Guidance Control and Dynamics1984，8，1： | 1 |
| 9 | Mucin gene (MUC1) transfered dendritic cells as vaccine: results of a phase I/II clinical trial显示文摘 | Pecher G Haling A Kaiser L | 2002 | Cancer Immunol Immunother2002，51，1112： | 1 |
| 10 | Pediatric obesity epidemiology 显示文摘 | Orsi CM Hale DE Lynch ]L | 2011 | Current Opinion in Endocrinology Diabetes &Obesity2011，18，： | 1 |
| 11 | Cycle and hor mone changes during perimenopause: the key role of ovarianfunction 显示文摘 | Burger HG Hale GE Dennerstein L | 2008 | Menopause2008，15，41： | 1 |
| 12 | Recurrent morphoeic basal carcinoma at the later canthus with orbitocranal invasion显示文摘 | Selva D Hale L Bouskill K | | 0，，02： | 1 |
| 13 | CCR5 antagonists:bicyclic isoxazolidines as conformationally constrained N-(l)-substituted pyrolidines显示文摘 | LYNCH C L GENTRY A L HALE J J | | 0，，04： | 1 |
| 14 | Effects of cement or lime on Cd, Co, Cu, Ni, Pb, Sb and Zn mobility in field-contaminated and aged soils 显示文摘 | Hale B Evans L Lambert R | 2012 | Journal of Hazardous Materials2012，，： | 1 |
| 15 | Modeling accidents for prioritizing prevention 显示文摘 | Hale A R Ale B J M Goossensa L H J | 2007 | Reliability Engineering and System Safety2007，92，12： | 1 |
| 16 | Induction of apoptosis in the germ cells of adult male rats after exposure to cyclophospha mide显示文摘 | Cai L Hales BF Robaire B | 1997 | Biol Reprod1997，56，6： | 1 |
| 17 | Use of thermal and vegetation index data from earth observing satelites to evaluate the risk of schistosomiasis in Bahia, Brazil 显示文摘 | Bavia ME Malone JB Hale L | 2001 | Acta Trop2001，79，1： | 1 |
| 18 | Heterosis for horticultural traits in Broccoli显示文摘 | Hale A L Farnham M W Nzaramba M N | 2007 | Theoretical and Applied Genetics2007，115，： | 1 |
| 19 | The development of an audit technique to assess the quality of safe-ty barrier management显示文摘 | Guldenmund F W Hale A R Goossens L H J | 2006 | Journal of Hazardous Materials2006，130，3： | 1 |
| 20 | Biomechanical and metabolic efforts of varying backpack loading on simulated marching 显示文摘 | Quesada P M Mengelkoch L J Hale R C | 2000 | Ergonomics2000，43，3： | 1 |
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