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| 1 | Prioritizing natural-selection signals from the deep-sequencing genomic data suggests multi-variant adaptation in Tibetan highlanders显示文摘Human genetic adaptation to high altitudes(>2500 m)has been extensively studied over the last few years,but few functional adaptive genetic variants have been identified,largely owing to the lack of deep-genome sequencing data available to previous studies.Here,w e build a list of putative adaptive variants,including 63 missense,7 loss-of-function;1,298 evolutionarily conserved variants and 509 expression quantitative traits loci.Notably,the top signal of selection is located in TMEM 247,a transmembrane protein-coding gene.The Tibetan version of TMEM 247 harbors one high-frequency(76.3%)missense variant,rsl 16983452(c.248C>T;p.Ala83Val);with the T allele derived from archaic ancestry and carried by>94%of Tibetans but absent or in low frequencies(<3%)in non-Tibetan populations.The rsl 16983452-T is strongly and positively correlated with altitude and significantly associated with reduced hemoglobin concentration(p=5.78×10^-5),red blood cell count(p=5.72×10^-7)and hematocrit(p=2.57×10^-6).In particular,TMEM247-rs 116983452 shows greater effect size and better predicts the phenotypic outcome than any E P A S1 variants in association with adaptive traits in Tibetans.Modeling the interaction between TM£M247-rsl 16983452 and EPAS1 variants indicates weak but statistically significant epistatic effects.Our results support that multiple variants may jointly deliver the fitness of the Tibetans on the plateau,where a complex model is needed to elucidate the adaptive evolution mechanism. | Lian Deng Chao Zhang Kai Yuan Yang Gao Yuwen Pan Xueling Ge Yaoxi He Yuan Yuan Yan Lu Xiaoxi Zhang Hao Chen Haiyi Lou Xiaoji Wang Dongsheng Lu Jiaojiao Liu Lei Tian Qidi Feng Asifullah Khan Yajun Yang Zi-Bing Jin Jian Yang Fan Lu Jia Qu Longli Kang Bing Su Shuhua Xu | 2019 | National Science Review2019，6，6： | 9 |
| 2 | Genome editing in Drosophila melanogaster: from basic genome engineering to the multipurpose CRISPR-Cas9 system显示文摘Nowadays, genome editing tools are indispensable for studying gene function in order to increase our knowledge of biochemical processes and disease mechanisms. The extensive availability of mutagenesis and transgenesis tools make Drosophila melanogaster an excellent model organism for geneticists. Early mutagenesis tools relied on chemical or physical methods,ethyl methane sulfonate(EMS) and X-rays respectively, to randomly alter DNA at a nucleotide or chromosomal level. Since the discovery of transposable elements and the availability of the complete fly genome, specific genome editing tools, such as P-elements, zinc-finger nucleases(ZFNs) and transcription activator-like effector nucleases(TALENs), have undergone rapid development. Currently, one of the leading and most effective contemporary tools is the CRISPR-cas9 system made popular because of its low cost, effectiveness, specificity and simplicity of use. This review briefly addresses the most commonly used mutagenesis and transgenesis tools in Drosophila, followed by an in-depth review of the multipurpose CRISPR-Cas9 system and its current applications. | Xingjie Ren Kristof Holsteens Haiyi Li Jin Sun Yifan Zhang Lu-Ping Liu Qingfei Liu Jian-Quan Ni | 2017 | Science China(Life Sciences)2017，60，5： | 5 |
| 3 | De novo assembly of a Tibetan genome and identification of novel structural variants associated with high-altitude adaptation显示文摘Structural variants(SVs) may play important roles in human adaptation to extreme environments such as high altitude but have been under-investigated. Here, combining long-read sequencing with multiple scaffolding techniques, we assembled a high-quality Tibetan genome(ZF1), with a contig N50 length of 24.57 mega-base pairs(Mb) and a scaffold N50 length of 58.80 Mb. The ZF1 assembly filled 80 remaining N-gaps(0.25 Mb in total length) in the reference human genome(GRCh38). Markedly, we detected17 900 SVs, among which the ZF1-specific SVs are enriched in GTPase activity that is required for activation of the hypoxic pathway. Further population analysis uncovered a 163-bp intronic deletion in the MKL1 gene showing large divergence between highland Tibetans and lowland Han Chinese. This deletion is significantly associated with lower systolic pulmonary arterial pressure, one of the key adaptive physiological traits in Tibetans. Moreover, with the use of the high-quality de novo assembly, we observed a much higher rate of genome-wide archaic hominid(Altai Neanderthal and Denisovan) shared non-reference sequences in ZF1(1.32%–1.53%) compared to other East Asian genomes(0.70%–0.98%),reflecting a unique genomic composition of Tibetans. One such archaic hominid shared sequence-a662-bp intronic insertion in the SCUBE2 gene-is enriched and associated with better lung function(the FEV1/FVC ratio) in Tibetans. Collectively, we generated the first high-resolution Tibetan reference genome, and the identified SVs may serve as valuable resources for future evolutionary and medical studies. | Ouzhuluobu Yaoxi He Haiyi Lou Chaoying Cui Lian Deng Yang Gao Wangshan Zheng Yongbo Guo Xiaoji Wang Zhilin Ning Jun Li Bin Li Caijuan Bai Baimakangzhuo Gonggalanzi Dejiquzong Bianba Duojizhuoma Shiming Liu Tianyi Wu Shuhua Xu Xuebin Qi Bing Su | 2020 | National Science Review2020，7，2： | 4 |
| 4 | Superconducting nanowire single-photon detectors:recent progress显示文摘Research of superconducting nanowire singlephoton detectors(SNSPDs) has been progressing rapidly in recent years. The combined properties of high efficiency,low noise, and fast speed of SNSPDs permit its applications ranging from long-distance quantum teleportation to moonto-earth optical communications. Here we briefly discussed recent progress of SNSPDs, in particular(1) tungstensilicide SNSPDs,(2) waveguide-integrated SNSPDs, and(3) a few applied demonstrations. | Xiaolong Hu Yuhao Cheng Chao Gu Xiaotian Zhu Haiyi Liu | 2015 | Science Bulletin2015，60，23： | 2 |
| 5 | Study on properties of ultra-low dielectric loss mPPO/MTCLT composites prepared by injection molding显示文摘A novel category of polyphenylene oxide/high-impact polystyrene(PPO/HIPS)alloy was used as the polymer matrix(abbreviated as mPPO)and loaded with different volume fractions(0,10,20,30,40,50 vol.%)of MgTiO_(3)-Ca_(0.7)La_(0.2)TiO_(3)(abbreviated as MTCLT)ceramics to prepare composites by injection molding.Its micromorphology,density,dielectric,thermal and mechanical properties were analyzed in detail.The experimental results show that the composites possess a compact microstructure because HIPS increases the fluidity of PPO.Due to the excellent dielectric properties of both mPPO and MTCLT,the composites have an extremely low dielectric loss.The realization of the high ceramic filler fraction greatly limits the thermal expansion of the polymer chain by introducing the interphase,so that the coefficient of thermal expansion of the composite material could be as low as 21.8 ppm/°C.At the same time,the presence of ceramic particles could reinforce the mechanical property of the compos-ites.When the ceramic filler fraction is higher than 20 vol.%,the bending strength of the composite material is around 110 MPa.When the ceramic filler fraction is 40 vol.%,the composite possesses the best comprehensive performance.The dielectric constant is 6.81,the dielectric loss is 0.00104,the thermal expansion coefficient is as low as 25.3 ppm/°C,and the bending strength is 110.4 MPa.Due to its excellent properties,this material can be a good candidate in the field of microwave communication. | Yahan Liu Haiyi Peng Xiaogang Yao Minmin Mao Kaixin Song Huixing Lin | 2022 | Journal of Advanced Dielectrics2022，12，3： | 1 |
| 6 | Implication of expression of osteopontin and its receptor integrin ανβ3 in the placenta in the development of preeclampsia显示文摘 | Junxia Xia Fuyuan Qiao Fangmin Su Haiyi Liu | 2009 | Journal of Huazhong University of Science and Technology2009，，6： | 1 |
| 7 | Phase III, randomized, open‐label, first‐line study in Asia of gefitinib versus carboplatin/paclitaxel in clinically selected patients with advanced non‐small‐cell lung cancer: evaluation of patients recruited from mainland China显示文摘 | Yi‐Long WU Da‐Tong CHU Baohui HAN Xuyi LIU Li ZHANG Caicum ZHOU Meilin LIAO Tony MOK Haiyi JIANG Emma DUFFIELD Masahiro FUKUOKA | 2012 | Asia‐Pacific Journal of Clinical Oncology2012，，3： | 1 |
| 8 | Ursodeoxycholic Acid in the Treatment of Intrabeptic Cholestasis of Pregnancy显示文摘 | LIU Yuling QIAO Fuyuan LIU Haiyi | 2006 | Journal of Huazhong University of Science and Technology [ Med Sci ]2006，26，3： | 1 |
| 9 | Spectroscopic analysis of high electric field enhanced ionization in laser filaments in air for corona guiding显示文摘We report on a systematic experimental study on the fluorescence spectra produced from a femtosecond laser filament in air under a high electric field. The electric field alone was strong enough to create corona discharge(CD). Fluorescence spectra from neutral and ionic air molecules were measured and compared with pure high-voltage CD and pure laser filamentation(FIL). Among them, high electric field assisted laser FIL produced nitrogen fluorescence more efficiently than either pure CD or pure FIL processes. The nonlinear enhancement of fluorescence from the interaction of the laser filament and corona discharging electric field resulted in a more efficient ionization along the laser filament zone, which was confirmed by the spectroscopic measurement of both ionization-induced fluorescence and plasma-scattered 800 nm laser pulses. This is believed to be the key precursor process for filament-guided discharge. | Yingxia Wei Yaoxiang Liu Tie-Jun Wang Na Chen Jingjing Ju Yonghong Liu Haiyi Sun Cheng Wang Jiansheng Liu Haihe Lu See Leang Chin Ruxin Li | 2016 | High Power Laser Science and Engineering2016，4，1： | 1 |
| 10 | Implication of expression of osteopontin and its receptor integrin ανβ3 in the placenta in the development of preeclampsia显示文摘 | Junxia Xia Fuyuan Qiao Fangmin Su Haiyi Liu | 2009 | Journal of Huazhong University of Science and Technology2009，，6： | 1 |
| 11 | Differential immune responses in pregnant patients recovered from COYID-19显示文摘Pregnant women are generally more susceptible to viral infection.Although the impact of SARS-CoV-2 in pregnancy remains to be determined,evidence indicates that the risk factors for severe COVID-19 are similar in pregnancy to the general population.Here we systemically analyzed the clinical characteristics of pregnant and non-pregnant female COVID-19 patients who were hospitalized during the same period and found that pregnant patients developed marked lymphopenia and higher inflammation evident by higher C-reactive protein and IL-6.To elucidate the pathways that might contribute to immunopathology or protective immunity against COVID-19 during pregnancy,we applied single-cell mRNA sequencing to profile peripheral blood mononuclear cells from four pregnant and six non-pregnant female patients after recovery along with four pregnant and three non-pregnant healthy donors.We found normal clonal expansion of T cells in the pregnant patients,heightened activation and chemotaxis in NK,NKT,and MAIT cells,and differential interferon responses in the monocyte compartment.Our data present a unique feature in both innate and adaptive immune responses in pregnant patients recovered from COVID-19. | Ge Chen Yiming Zhang Yaoyao Zhang Jihui Ai Bin Yang Mengge Cui Qiuyue Liao Hanxiao Chen Hualin Bai Dashing Shang Jing Chen ChaoYang Sun Haiyi Liu Fengyuan Liu Bin Mao Guoqiang Sun Lu Chen Jing-wen Lin Kezhen Li | 2021 | Signal Transduction and Targeted Therapy2021，6，8： | 0 |
| 12 | Observation and analysis of morphology abnormalities in development of Oryzias melastigma embryos显示文摘Fish embryos are widely used as models in toxicology,drug development,and human disease research because of their high sensitivity,observability,and operability,providing the basis for an in-depth understanding of the embryogenesis.Increasing studies have indicated that birth defects are hereditary.In this study,we used Oryzias melastigma as a model to conduct a study of 185-day embryogenesis and observed self-induced non-pathological abnormal embryogenesis.O.melastigma experienced pre-puberty stage,adolescence stage,and senescence stage,and individuals produced up to 102 eggs per day.However,the fecundity was markedly reduced during the senescent stage.During the active egg and blastodisc stages,pseudo-fertilization and pseudo-blastocysts were observed.During cleavage at the 4-to 32-cell stages,we observed blastomeres separation or dislocation.Excessively separated blastomeres formed double blastoderms,eventually resulting in conjoined twins.During the blastula stage,we observed abnormally increased cell volume,narrowed and elongated blastocysts,and abnormally coated blastoderms.At the organogenesis stage,we observed abnormal numbers of Kupff er’s vesicles and conjoined twins.Abnormality in the location and number of oil droplets were observed in various development stages.Abnormal development was more commonly observed in fertilized eggs produced by broodstock in pre-puberty or senescence stages,which is probably related to the age of fish and the egg quality.This study can provide the materials for comparative analysis in toxicological and molecular studies of O.melastigma,and may provide evidence for other economic fish that produce sticky eggs. | Jingjing SONG Haiyi WU Kaikai LIU Wendan CHI Shanshan GE Zhipeng ZHANG Mingqi WANG Yi YU Daode YU | 2021 | Journal of Oceanology and Limnology2021，39，4： | 0 |
| 13 | Phase Ⅱ study of mitomycin C and cisplatin in heavily pretreated advanced breast cancer显示文摘Objective:The mitomycin C and cisplatin combination was investigated in patients with advanced breast cancer who had been exposed to anthracyclines,vinorelbine and taxanes.Methods:Three-weekly regimen consisted of mitomycin,6 mg/m2 administered intravenously on day 1,and cisplatin,25 mg/m2 intravenously on day 1-3.Results:Thirty-eight patients aged 25-75 years(median,46 years)were treated with an overall response rate of 31.6%.The median time to progression(TTP)was 4.0 months.Median TTP for 12 patients with a complete or partial response was 9.0 months,while stable disease and progression of disease 4.0 months,P=0.002.Grade 3/4 side effects of neutropenia,thrombocytopenia and nausea/vomit-ing were documented in 4(10.5%),4(10.5%)and 3(7.9%)patients,respectively.The median overall survival was 13+ months.Conclusion:Mitomycin C/cisplatin doublet showed antitumor activity for anthracycline-,vinorelbine-and taxane-resistant breast cancer comparable to other regimens.This well-tolerated regimen provides an affordable option for patients in China. | Xichun Hu Haiyi Guo Xinmin Zhao Zhonghua Wang Xinmiao Yang Xiangjin Liu Jin Li | 2006 | The Chinese-German Journal of Clinical Oncology2006，5，6： | 0 |
| 14 | Pericytes may facilitate SARS-CoV-2 entry into the nervous system显示文摘Objective:Although the neurological and olfactory symptoms of coronavirus disease 2019 have been identified,the neurotropic properties of the causative virus,severe acute respiratory syndrome-associated coronavirus 2(SARS-CoV-2),remain unknown.We sought to identify the susceptible cell types and potential routes of SARS-CoV-2 entry into the central nervous system,olfactory system,and respiratory system.Methods:We collected single-cell RNA data from normal brain and nasal epithelium specimens,along with bronchial,tracheal,and lung specimens in public datasets.The susceptible cell types that express SARS-CoV-2 entry genes were identified using single-cell RNA sequencing and the expression of the key genes at protein levels was verified by immunohistochemistry.We compared the coexpression patterns of the entry receptor angiotensin-converting enzyme 2(ACE2)and the spike protein priming enzyme transmembrane serine protease(TMPRSS)/cathepsin L among the specimens.Results:The SARS-CoV-2 entry receptor ACE2 and the spike protein priming enzyme TMPRSS/cathepsin L were coexpressed by pericytes in brain tissue;this coexpression was confirmed by immunohistochemistry.In the nasal epithelium,ciliated cells and sustentacular cells exhibited strong coexpression of ACE2 and TMPRSS.Neurons and glia in the brain and nasal epithelium did not exhibit coexpression of ACE2 and TMPRSS.However,coexpression was present in ciliated cells,vascular smooth muscle cells,and fibroblasts in tracheal tissue;ciliated cells and goblet cells in bronchial tissue;and alveolar epithelium type 1 cells,AT2 cells,and ciliated cells in lung tissue.Conclusion:Neurological symptoms in patients with coronavirus disease 2019 could be associated with SARS-CoV-2 invasion across the blood-brain barrier via pericytes.Additionally,SARS-CoV-2-induced olfactory disorders could be the result of localized cell damage in the nasal epithelium. | Zijian Kang Jing Wang Tong Meng Hao Zhang Da Xu Haiyi Gong Zhenyan Chang Zifu Li Xingang Cui Jianru Xiao Adnan I.Qureshi Wang Zhou Jianmin Liu Huji Xua | 2023 | Journal of Bio-X Research2023，6，1： | 0 |
| 15 | Laboratory Model Tests and DEM Simulations of Unloading-Induced Tunnel Failure Mechanism显示文摘Tunnel excavation is a complicated loading-unloading-reloading process characterized by decreased radial stresses and increased axial stresses.An approach that considers only loading,is generally used in tunnel model testing.However,this approach is incapable of characterizing the unloading effects induced by excavation on surrounding rocks and hence presents radial and tangential stress paths during the failure process that are different from the actual stress state of tunnels.This paper carried out a comparative analysis using laboratory model testing and particle flow code(PFC2D)-based numerical simulation,and shed light upon the crack propagation process and,microscopic stress and force chain variations during the loading-unloading process.The failure mode observed in the unloading model test is shear failure.The force chains are strongly correlated with the concrete fracture propagation.In addition,the change patterns of the radial and tangential stresses of surrounding rocks in the broken region,as well as the influence of the initial stress on failure loads are revealed.The surrounding soil of tunnel failure evolution as well as extent and shape of the damage zone during the excavation-induced unloading were also studied. | Abierdi Yuzhou Xiang Haiyi Zhong Xin Gu Hanlong Liu Wengang Zhang | 2020 | Computers, Materials & Continua2020，，5： | 0 |
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