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| 1 | Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain显示文摘The full-length cDNA clone of swine vesicular disease virus HK/70 strain named pSVOK12 was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. In vitro transcription RNA from pSVOK12 transfected IBRS-2 cells and the re- covered virus RNA were isolated and sequenced, then indirect hemagglutination test, indirect im- munofluorescence assays, eleectron microscope test, 50% tissue culture infecting dose (TCID50) assays and mouse virulence studies were performed to study the antigenicity and virulence of the recovered virus. The result showed that the infectious clones we ob- tained and the virus derived from pSVOK12 had the same biological properties as the parental strain HK/70. The full-length infectious cDNA clone, pSVOK12, will be very useful in studies of the anti- genicity, virulence, pathogenesis, maturation and replication of SVDV. | ZHENG Haixue LIU Xiangtao SHANG Youiun WU Jinyan BAI Xingwen JIN Ye SUN Shiqi GUO Huichen TIAN Hong FENG Xia YIN Shuanghui GUO Jianhong CONG Guozheng LIU Zaixin CHANG Huiyun MA Junwu XIE Qingge | 2006 | Chinese Science Bulletin2006，51，17： | 6 |
| 2 | Tracing of dye molecules in living plants through NaGdF_4:Yb^(3+),Er^(3+)fluorescent nanoprobes显示文摘In this paper, we demonstrated trace of dye molecules in living plants. The NaGdF4:Yb^(3+),Er^(3+) nanoparticles probe was used to detect the rhodamine B(RhB) in bean sprout. It is found that the fluorescencedye can be efficiently imbibed during the growing process and the absorbance presented a position dependence effect, which was supported by the upconversion spectra and the fluorescent image characterization. In addition, the concentration of the residual RhB in bean sprout can be efficiently traced by the synthesized probe based on the fluorescent resonant energy transfer. Finally, the relation between the excitation power, concentration and the ratio of yellow to green emission are discussed in detail. These results can be helpful in understanding the RhB dye molecules absorbance process in vegetable growth and provide an efficient way to trace the residual dyes in vivo plant. | Xiaofeng Wu Ya'nan Zhang Shiping Zhan Jin Li Guozheng Nie Shigang Hu Cong Yan Shaobing Wu Shengbin Cheng Junshan Hu Lichun Shi Yunxin Liu | 2019 | Journal of Rare Earths2019，37，3： | 3 |
| 3 | Complete nucleotide sequence of a Chinese serotype Asia1 vaccine strain of foot-and-mouth disease virus显示文摘 | Junzheng Du Huiyun Chang Guozheng Cong Junjun Shao Tong Lin Youjun Shang Zaixin Liu Xiangtao Liu Xuepeng Cai Qingge Xie | 2007 | Virus Genes2007，，3： | 1 |
| 4 | Generation of Monoclonal Antibodies against Non-structural Protein 3AB of Foot-and-Mouth Disease Virus显示文摘To identify linear epitopes on the non-structural protein 3AB of foot-and-mouth disease virus (FMDV), BABL/c mice were immunized with the 3AB protein and splenocytes of BALB/c mice were fused with myeloma Sp2/0 cells. Two hybridoma monoclonal antibodies (mAbs) cell lines against the 3AB protein of foot-and-mouth disease virus (FMDV) were obtained, named C6 and E7 respectively . The microneutralization titer was 1:1024 for mAb C6, and 1:512 for E7. Both mAbs contain kappa light chains, and were of subclass IgG2b. In order to define the mAbs binding epitopes, the reactivity of these mAbs against FMDV were examined by indirect ELISA. The results showed that both mAbs can react with FMDV, but had no cross-reactivity with Swine Vesicular Disease (SVD) antigens. The titers in abdomen liquor were 1:5×106 for C6 and 1:2×106 for E7. In conclusion, the mAbs obtained from this study are specific for the detection of FMDV, can be used for etiological and immunological researches on FMDV, and have potential use in diagnosis and future vaccine designs. | Tong Lin Junjun Shao Huiyun Chang Shandian Gao Guozheng Cong Junzheng Du | 2012 | Virologica Sinica2012，27，5： | 0 |
| 5 | Rethinking development and major research plans of Industrial Internet in China显示文摘This paper gives a definition of the Industrial Internet and expounds on the academic connotation of the future Industrial Internet.From this foundation,we outline the development and current status of the Industrial Internet in China and globally.Moreover,we detail the avant-garde paradigms encompassed within the National Natural Science Foundation of China(NSFC)’s“Future Industrial Internet Fundamental Theory and Key Technologies”research plan and its corresponding management strategies.This research initiative endeavors to enhance interdisciplinary collaborations,aiming for a synergistic alignment of industry,academia,research,and practical implementations.The primary focus of the research plan is on the pivotal scientific challenges inherent to the future industrial internet.It is poised to traverse the“first mile”,encompassing foundational research and pioneering innovations specific to the industrial internet,and seamlessly bridges to the“last mile”,ensuring the effective commercialization of scientific and technological breakthroughs into tangible industrial market applications.The anticipated contributions from this initiative are projected to solidify both the theoretical and practical scaffolding essential for the cultivation of a globally competitive industrial internet infrastructure in China. | Chunxiao Jiang Yang Cong Jiming Chen Chenghong Wang Guozheng Wu Ruizhen Zhao Zhiheng Wang Bin Xiao Ting Chen | 2024 | Fundamental Research2024，4，1： | 0 |
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