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| 1 | Food synthetic biology-driven protein supply transition: From animal-derived production to microbial fermentation显示文摘Animal-derived protein production is one of the major traditional protein supply methods,which continues to face increasing challenges to satisfy global needs due to population growth,augmented individual protein consumption,and aggravated environmental pollution.Thus,ensuring a sustainable protein source is a considerable challenge.The emergence and development of food synthetic biology has enabled the establishment of cell factories that effectively synthesize proteins,which is an important way to solve the protein supply problem.This review aims to discuss the existing problems of traditional protein supply and to elucidate the feasibility of synthetic biology in the process of protein synthesis.Moreover,using artificial bioengineered milk and artificial bioengineered eggs as examples,the progress of food protein supply transition based on synthetic biology has been systematically summarized.Additionally,the future of food synthetic biology as a potential source of protein has been also discussed.By strengthening and innovating the application of food synthetic biology technologies,including genetic engineering and high-throughput screening methods,the current limitations of artificial foods for protein synthesis and production should be addressed.Therefore,the development and industrial production of new food resources should be explored to ensure safe,high-quality,and sustainable global protein supply. | Yanfeng Liu Xiaomin Dong Bin Wang Rongzhen Tian Jianghua Li Long Liu Guocheng Du Jian Chen | 2021 | Chinese Journal of Chemical Engineering2021，34，2： | 4 |
| 2 | An effective cytokine combination for ex vivo expansion of porcine muscle stem cells显示文摘Cultured meat technology is a novel and promising strategy for sustainable and effective meat production.Muscle stem cells are widely used as seed cell populations because of their ease of access and myogenic differentiation potential.However,muscle stem cells are difficult to continuously propagate ex vivo and are heavily dependent upon serum for survival and growth,which impedes their commercial use as a cultured meat source.Herein,we identified an effective four-cytokine combination to promote long-term proliferation of porcine muscle stem cells using a serial elimination screening approach,which was consisted of long chain human insulin growth factor-1,platelet derived growth factor BB,basic fibroblast growth factor,and epidermal growth factor.The expansion of muscle stem cells with the four-cytokine combination achieved a 6.31×107-fold increase in cell number after 28 days of culture with retained cell myogenic differentiation potential,and most importantly,reduced the amount of fetal bovine serum required for cell culture by at least 5%.Furthermore,the four-cytokine combination exerted the pro-proliferative function by activating PI3K/Akt/mTOR and MEK/ERK signaling pathways.This approach provides for a new means by which to industrialize the production of cultured meat. | Qingzi Lei Mei Li Guocheng Du Jingwen Zhou Xin Guan | 2022 | Food Bioscience2022，46，2： | 3 |
| 3 | Enhancement of solubilization and acidification of waste activated sludge by pretreatment显示文摘 | Xiaoling Liu He Liu Jinhuan Chen Guocheng Du Jian Chen | 2008 | Waste Management2008，，12： | 2 |
| 4 | Metabolic engineering of Escherichia coli for the production of Lacto-N-neotetraose(LNnT)显示文摘Lacto-N-neotetraose(LNnT),one of the most important human milk oligosaccharides,can be used as infants’food addi-tives.Nowadays,extraction,chemical and biological synthesis were utilized to obtain LNnT,while these methods still face some problems such as low yield and high cost.The aim of current work is to construct a de novo biosynthesis pathway of LNnT in E.coli K12 MG1655.The lgtA and lgtB were first expressed by a plasmid,resulting in a LNnT titer of 0.04 g/L.To improve the yield of LNnT on substrate lactose,lacZ and lacI were knocked out,and lacY was over-expressed.As a result,the yield of LNnT on lactose increased from 0.01 to 0.09 mol/mol,and the titer of LNnT elevated to 0.41 g/L.In addition,the pathway was regulated using the titer of Lacto-N-triose II(LNTII)as a measure,and obtained a high titer strain of LNnT for 1.04 g/L.Finally,the gene expressions were fine-tuned,the titer of LNnT reached 1.2 g/L,which was 93%higher than the control strain,and the yield on lactose reached 0.28 mol/mol.The engineering strategy of pathway construction and modulation used in this study is applicable to facilitate the microbial production of other metabolites in E.coli. | Wei Zhang Zhenmin Liu Mengyue Gong Nan Li Xueqin Lv Xiaomin Dong Yanfeng Liu Jianghua Li Guocheng Du Long Liu | 2021 | Systems Microbiology and Biomanufacturing2021，1，3： | 2 |
| 5 | Recent advances and challenges in microbial production of human milk oligosaccharides显示文摘Human milk oligosaccharides(HMOs)are one of the major differences between livestock milk and human milk,and the prebiotic functions of HMOs have been verified through in vitro and clinical trials.The most abundant HMOs include 2′-fucysollactose(2′-FL),3-fucosyllactose(3-FL),lacto-N-neotetraose(LNnT)and lacto-N-tetraose(LNT);their application and synthesis have attracted wide attentions.In recent years,the biotechnological production of 2′-FL,3-FL,LNnT and LNT have emerged based on techniques such as whole-cell catalysis and fermentation.In particular,the development of metabolic engineering and synthetic biology methods and strategies have facilitated efficient biosynthesis of these HMOs.However,these advantages have not been systematically reviewed yet.In this review,we first discuss the structures and applications of HMOs;secondly,strategies of microbial synthesis of the most abundant 2′-FL,3-FL,LNnT and LNT are summarized and compared.Finally,challenges and perspectives of efficient microbial production of HMOs as well as strategies for overcoming the challenges are discussed.This review reveals the whole picture of recent development in HMOs microbial synthesis and can further facilitate the understanding of limiting factors,and further propose a few directions to promote the development of efficient production hosts. | Jieying Deng Xueqin Lv Jianghua Li Guocheng Du Jian Chen Long Liu | 2021 | Systems Microbiology and Biomanufacturing2021，1，1： | 2 |
| 6 | Current advances in design and engineering strategies of industrial enzymes显示文摘Enzymes are considered as functional proteins,also known as biocatalysts,which are required for normal cellular function.The commercial production of enzymes with distinct features always remains the top priority of researchers for industrial biocatalysis.However,the insufficient yield,low stability,low activity,formation of by-products,complex purification pro-cess,and many other factors are challenging for the enzyme production industry.Novel advancements in protein engineering,especially the combination of multidisciplinary techniques including post-translational enzyme modification,structured assisted protein tailoring and computational modeling approaches,open a new horizon to more efficient production of bio-catalysts.In this review,we focus on the development process of enzyme modification design strategies,such as rational design,semi-rational design and de novo design.Selected examples for each strategy,which are particularly useful for novel enzyme design,are given.We hope this review is helpful for the development of biocatalysts for industrial application. | Tanatarov Dinmukhamed Ziyang Huang Yanfeng Liu Xueqin Lv Jianghua Li Guocheng Du Long Liu | 2021 | Systems Microbiology and Biomanufacturing2021，1，1： | 2 |
| 7 | Characteristic and correlation analysis of influent and energy consumption of wastewater treatment plants in Taihu Basin显示文摘The water quality and energy consumption of wastewater treatment plants(WWTPs)in Taihu Basin were evaluated on the basis of the operation data from 204 municipal WWTPs in the basin by using various statistical methods.The influent ammonia nitrogen(NH3-N)and total nitrogen(TN)of WWTPs in Taihu Basin showed normal distribution,whereas chemical oxygen demand(COD),biochemical oxygen demand(BOD5),suspended solid(SS),and total phosphorus(TP)showed positively skewed distribution.The influent BOD5/COD was 0.4%-0.6%,only 39.2%SS/BOD5 exceeded the standard by 36.3%,the average BOD5/TN was 3.82,and the probability of influent BOD5/TP>20 was 82.8%.The average energy consumption of WWTPs in Taihu Basin in 2017 was 0.458 kWh/m^3.The specific energy consumption of WWTPs with a daily treatment capacity of more than 5×10^4 m^3 in Taihu Basin was stable at 0.33 kWh/m^3.A power function relationship was observed between the reduction in COD and NH3-N and the specific energy consumption of pollutant reduction,and the higher the pollutant reduction is,the lower the specific energy consumption of pollutant reduction presents.In addition,a linear relationship existed between the energy consumption of WWTPs and the specific energy consumption of influent volume and pollutant reduction.Therefore,upgrading and operation with less energy consumption of WWTPs is imperative and the suggestions for Taihu WWTPs based on stringent discharge standard are proposed in detail. | Luxi Zou Huaibo Li Shuo Wang Kaikai Zheng Yan Wang Guocheng Du Ji Li | 2019 | Frontiers of Environmental Science & Engineering2019，13，6： | 2 |
| 8 | Purification and characterization of transglutaminase from a newly isolated Stretomyces hygroscopicu显示文摘 | Cui Li Du Guocheng Zhang Dongxu | 2007 | Food Chemistry2007，4，： | 1 |
| 9 | Application of response surface methodology in medium optimization for spore production of Coniothyrium minitans in solid-state fermentation显示文摘 | Chen Xin Li Yin Du Guocheng | 2005 | World Journal of Microbiology & Biotechnology2005，21，4： | 1 |
| 10 | A temperatureshift strategy in batch microbial transglutaminase fermentation显示文摘 | ZHENG Meiying DU Guocheng GUO Wangfang | 2001 | Process Biochemistry2001，36，6： | 1 |
| 11 | Novel fermentationprocesses for manufacturing plant natural products 显示文摘 | Zhou Jingwen Du Guocheng Chen Jian | 2014 | Current Opinion in Biotechnology2014，25，： | 1 |
| 12 | Enhancement of solubilization and acidification of waste activated sludge by pretreatment显示文摘 | Xiaoling Liu He Liu Jinhuan Chen Guocheng Du Jian Chen | 2008 | Waste Management2008，，12： | 1 |
| 13 | Effect of trehalose,transglutaminase, and gum on rheological, fermentation, and baking properties of frozen dough显示文摘 | KIM Y S HUANG Weining DU Guocheng | 2008 | Food Research International2008，41，： | 1 |
| 14 | Influence of aeration intensity on the performance of A/0-type sequencing batch MBR system treating azo dye wastewater显示文摘Among the numerous parameters affecting the membrane bioreactor(MBR)performance,the aeration intensity is one of the most important factors.In the present investigation,an anoxic/aerobic-type(A/O-type)sequencing batch MBR system,added anoxic process as a pretreatment to improve the biodegradability of azo dye wastewater,was investigated under different aeration intensities and the impact of the aeration intensity on effluent quantity,sludge properties,extracellular polymeric substances(EPS)amount generated as well as the change of permeation flux were examined.Neither lower nor higher aeration intensities could improve A/O-type sequencing batch MBR performances.The results showed 0.15 m^(3)·h^(-1)aeration intensity was promising for treatment of azo dye wastewater under the conditions examined.Under this aeration intensity,chemical oxygen demand(COD),ammonium nitrogen and color removal as well as membrane flux amounted to 97.8%,96.5%,98.7%and 6.21 L·m^(-2)·h^(-1),respectively.The effluent quality,with 25.0 mg·L^(-1)COD,0.84 mg·L^(-1)ammonium nitrogen and 8 chroma,could directly meet the reuse standard in China.In the meantime,the sludge relative hydrophobicity,the bound EPS,soluble EPS and EPS amounts contained in the membrane fouling layer were 70.3%,52.0 mg·g^(-1)VSS,38.8 mg·g^(-1)VSS and 90.8 mg·g^(-1)VSS,respectively,which showed close relationships to both pollutant removals and membrane flux. | Xinhua WANG Jingmei LI Xiufen LI Guocheng DU | 2011 | Frontiers of Environmental Science & Engineering2011，5，4： | 1 |
| 15 | Comparative study on the influence of dissolved oxygen control approaches on the microbial hyaluronic acid production of Streptococcus zooepidemicus显示文摘 | Liu Long Du Guocheng Chen Jian | | 0，，06： | 1 |
| 16 | Meta- bolic engineering of Escherichia coli for (2S)-pinocem- brin production from glucose by a modular metabolic strate- gy显示文摘 | Wu Junjun Du Guocheng Zhou Jingwen | 2013 | Metabolic Engineering2013，16，： | 1 |
| 17 | Modu- lar optimization of heterologous pathways for de novo synthesis of (2S)-naringenin in Escherichia coli显示文摘 | Wu Junjun Zhou Tiantian Du Guocheng | 2014 | PloS One2014，9，10： | 1 |
| 18 | Application of response surface methodology in medium optimization for spore production of Coniothyrium minitans in solid-state fermentation显示文摘 | Chen Xin Li Yin Du Guocheng | | 0，，4： | 1 |
| 19 | Application of response surface methodology in medium optimization for spore production of Coniothyrium minitans in solid-state fermentation显示文摘 | Xin Chen Yin Li Guocheng Du | 2005 | World Journal of Microbiology & Biotechnology2005，21，： | 1 |
| 20 | Role of nitrate in biological phosphorus removal in a sequencing batch reactor显示文摘 | ZOU Hua DU Guocheng RUAN Wenquan | 2006 | World Journal of Microbiology and Biotechnology2006，22，7： | 1 |
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