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| 1 | Cytotoxic Effect on Osteosarcoma MG-63 Cells by Degradation of Magnesium显示文摘Biocompatible and biodegradable magnesium(Mg)based metals have attracted great interest for use in orthopedic implants and devices.Based on our previous study that Mg with and without micro-arc oxidation(MAO)coating showed obvious cytotoxic effect on tumor cells due to the increase of pH value during the degradation of Mg,this study further evaluated the cytotoxic effect of Mg and MAO coated Mg on osteosarcoma(MG-63)cells by analyzing the cell adhesion,morphology and number through observation of scanning electron microscope,as well as live/dead staining,lactate dehydrogenase(LDH)activity and 4,6-diamidino-2-phenylindole(DAPI)assay.The results indicated that,compared to titanium,Mg could strongly inhibit the cell adherence,morphology and number of MG-63 on the surface of the naked Mg,whereas the MAO coated Mg showed relative weak cytotoxic effect on MG-63 cells,expecting that magnesium based metals with suitable coating can be designed to be applied as tumor prosthesis in the clinical practice. | Mei Li Ling Ren LiHua Li Peng He GuoBo Lan Yu Zhang Ke Yang | 2014 | Journal of Materials Science & Technology2014，30，9： | 7 |
| 2 | Promoting Bone Mesenchymal Stem Cells and Inhibiting Bacterial Adhesion of Acid-Etched Nanostructured Titanium by Ultraviolet Functionalization显示文摘Titanium(Ti) and its alloys are used extensively in orthopedic implants because of their excellent biocompatibility,mechanical properties and corrosion resistance. However,titanium-based implant materials face many severe complications,such as implant loosening due to poor osseointegration and bacterial infections,which may lead to implant failure. Hence,preparing a biomaterial surface,which enhances the interactions with host cells and inhibits bacterial adhesion,may be an optimal strategy to reduce the incidence of implant failure. This study aims to improve osseointegration and confer antibacterial properties on Ti through a combination of two surface modifications including nanostructuring generated by acid etching and ultraviolet(UV) light treatment.Our results showed that without UV treatment,the acid etching treatment of Ti surface was effective at both improving the adhesion of bone mesenchymal stem cells(BMSCs) and increasing bacterial adhesion. A further UV treatment of the acid-etched surface however,not only significantly improved the cell adhesion but also inhibited bacterial adhesion. The acid-etched nanostructured titanium with UV treatment also showed a significant enhancement on cell proliferation,alkaline phosphatase(ALP) activity and mineralization. These results suggest that such nanostructured materials with UV treatment can be expected to have a good potential in orthopedic applications. | Guobo Lan Mei Li Ying Tan Lihua Li Xiaoming Yang Limin Ma Qingshui Yin Hong Xia Yu Zhang Guoxin Tan Chengyun Ning | 2015 | Journal of Materials Science & Technology2015，31，2： | 7 |
| 3 | Quantitativegeneticanalysisstationforthegeneticanalysisofcomplextraits显示文摘The Quantitative Genetic Analysis Station (QGAStation) is a software package that has been developed to perform statistical analysis for complex traits.It consists of five domains for handling data from diallel crosses,regional trials,core germplasm collections,QTL mapping,and microarray experiments.The first domain contains genetic models for diallel cross analysis,in which genetic variance components and genetic-by-environment interactions can be estimated,and genetic effects can be predicted.The second domain evaluates the performance of varieties in regional trials by implementing a general statistical method that outperforms ANOVA in tackling unbalanced data that arises frequently in trials across multiple locations and over a number of years.The third domain,using predicted genotypic values as proxy,constructs core germplasm collections covering sufficient genetic diversity with lower redundancy.The fourth domain manages genotypic and phenotypic data for QTL mapping.Linkage maps can be constructed and genetic distances can be estimated;the statistical methods that have been implemented apply to both chiasmatic and achiasmatic organisms.Another part of this domain can filter systematic noises in phenotypic data.The fifth domain focuses on the cDNA expression data that is generated by microarray experiments.A two-step strategy has been implemented to detect differentially expressed genes and to estimate their effects.Except in the fourth domain,the major statistical methods that have been used are mixed linear model approaches that have been implemented in the C language.Computational efficiency is further boosted for computers that are equipped with graphics processing units (GPUs).A user friendly graphic interface is provided for Microsoft Windows and Apple Mac operating systems.QGAStation is available at http://gffzzbe43f054107d49d7hqfvvuxx6vxnv6f05.ffgz.tsg.suse.edu.cn/software/qga/. | CHEN GuoBo ZHU ZhiXiang ZHANG FuTao ZHU Jun | 2012 | Chinese Science Bulletin2012，57，21： | 6 |
| 4 | NAD+metabolism:pathophysiologic mechanisms and therapeutic potential显示文摘Nicotinamide adenine dinucleotide(NAD^(+))and its metabolites function as critical regulators to maintain physiologic processes,enabling the plastic cells to adapt to environmental changes including nutrient perturbation,genotoxic factors,circadian disorder,infection,inflammation and xenobiotics.These effects are mainly achieved by the driving effect of NAD^(+)on metabolic pathways as enzyme cofactors transferring hydrogen in oxidation-reduction reactions.Besides,multiple NAD^(+)-dependent enzymes are involved in physiology either by post-synthesis chemical modification of DNA,RNA and proteins,or releasing second messenger cyclic ADPribose(cADPR)and NAADP^(+).Prolonged disequilibrium of NAD^(+)metabolism disturbs the physiological functions,resulting in diseases including metabolic diseases,cancer,aging and neurodegeneration disorder.In this review,we summarize recent advances in our understanding of the molecular mechanisms of NAD^(+)-regulated physiological responses to stresses,the contribution of NAD^(+)deficiency to various diseases via manipulating cellular communication networks and the potential new avenues for therapeutic intervention. | Na Xie Lu Zhang Wei Gao Canhua Huang Peter Ernst Huber Xiaobo Zhou Changlong Li Guobo Shen Bingwen Zou | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 5 | Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells显示文摘Neutrophil extracellular traps(NETs)can capture and kill viruses,such as influenza viruses,human immunodeficiency virus(HIV),and respiratory syncytial virus(RSV),thus contributing to host defense.Contrary to our expectation,we show here that the histones released by NETosis enhance the infectivity of SARS-CoV-2,as found by using live SARS-CoV-2 and two pseudovirus systems as well as a mouse model.The histone H3 or H4 selectively binds to subunit 2 of the spike(S)protein,as shown by a biochemical binding assay,surface plasmon resonance and binding energy calculation as well as the construction of a mutant S protein by replacing four acidic amino acids.Sialic acid on the host cell surface is the key molecule to which histones bridge subunit 2 of the S protein.Moreover,histones enhance cell-cell fusion.Finally,treatment with an inhibitor of NETosis,histone H3 or H4,or sialic acid notably affected the levels of sgRNA copies and the number of apoptotic cells in a mouse model.These findings suggest that SARS-CoV-2 could hijack histones from neutrophil NETosis to promote its host cell attachment and entry process and may be important in exploring pathogenesis and possible strategies to develop new effective therapies for COVID-19. | Weiqi Hong Jingyun Yang Jun Zou Zhenfei Bi Cai He Hong Lei Xuemei He Xue Li Aqu Alu Wenyan Ren Zeng Wang Xiaohua Jiang Kunhong Zhong Guowen Jia Yun Yang Wenhai Yu Qing Huang Mengli Yang Yanan Zhou Yuan Zhao Dexuan Kuang Junbin Wang Haixuan Wang Siyuan Chen Min Luo Ziqi Zhang Tiangi Lu Li Chen Haiying Que Zhiyao He Qiu Sun Wei Wang Guobo Shen Guangwen Lu Zhiwei Zhao Li Yang Jinliang Yang Zhenling Wang Jiong Li Xiangrong Song Lunzhi Dai Chong Chen Jia Geng Maling Gou Lu Chen Haohao Dong Yong Peng Canhua Huang Zhiyong Qian Wei Cheng Changfa Fan Yuquan Wei Zhaoming Su Aiping Tong Shuaiyao Lu Xiaozhong Peng Xiawei Wei | 2022 | Cellular & Molecular Immunology2022，19，5： | 2 |
| 6 | New techniques and strategies in drug discovery显示文摘Great success has been witnessed in last decades,some new techniques and strategies have been widely used in drug discovery.In this roadmap,several representative techniques and strategies are highlighted to show recent advances in this filed.(A)A DOX protocol has been developed for accurate protein-ligand binding structure prediction,in which first principle method was used to rank the binding poses.Validation against crystal structures have found that DOX prediction achieved an impressive success rate of 99%,indicating significant improvement over molecular docking method.(B)Virtual target profiling is a compound-centric strategy enabling a parallel implementation of interrogating compounds against various targets in a single screen,which has been used in hit/lead identification,drug repositioning,and mechanism-of-action studies.Current and emerging methods for virtual target profiling are briefly summarized herein.(C)Research on targeted autophagy to treat diseases has received encouraging progress.However,due to the complexity of autophagy and disease,experimental and in silico methods should be performed synergistically for the entire process.This part focuses on in silico methods in autophagy research to promote their use in medicinal research.(D)Histone deacetylases(HDACs)play important roles in various biological functions through the deacetylation of lysine residues.Recent studies demonstrated that HDACs,which possess low deacetylase activities,exhibited more efficient defatty-acylase activities.Here,we review the defatty-acylase activity of HDACs and describe examples for the design of isoform selective HDAC inhibitor.(E)The FDA approval of three kinase allosteric inhibitors and some others entering clinical study has spurred considerable interests in this targeted drug discovery area.(F)Recent advances are reviewed in structure-based design of novel antiviral agents to combat drug resistance.(G)Since nitric oxide(NO)exerts anticancer activity depending on its concentration,optimal levels of NO in cancer cells is desirable.In this minireview,we briefly describe recent advances in the research of NO-based anticancer agents by our group and present some opinions on the future development of these agents.(H)The field of photoactivation strategies have been extensively developed for controlling chemical and biological processes with light.This review will summarize and provide insight into recent research advances in the understanding of photoactivatable molecules including photoactivatable caged prodrugs and photoswitchable molecules. | Jintong Du Jing Guo Dongwei Kang Zhihong Li Guan Wang Jianbing Wu Zhen Zhang Hao Fang Xuben Hou Zhangjian Huang Guobo Li Xiaoyun Lu Xinyong Liu Liang Ouyang Li Rao Peng Zhan Xiaojin Zhang Yihua Zhang | 2020 | Chinese Chemical Letters2020，31，7： | 1 |
| 7 | Laser chirp controlled relativistic few-cycle mid-infrared pulse generation显示文摘Relativistic few-cycle mid-infrared(mid-IR)pulses are unique tools for strong-field physics and ultrafast science,but are difficult to generate with traditional nonlinear optical methods.Here,we propose a scheme to generate such pulses with high efficiency via plasma-based frequency modulation with a negatively chirped laser pulse(NCLP).The NCLP is rapidly compressed longitudinally due to dispersion and plasma etching,and its central frequency is downshifted via photon deceleration due to the enhanced laser intensity and plasma density modulations.Simulation results show that few-cycle mid-IR pulses with the maximum center wavelength of 7.9µm and pulse intensity of a_(MIR)=2.9 can be generated under a proper chirp parameter.Further,the maximum energy conversion efficiency can approach 5.0%.Such a relativistic mid-IR source is promising for a wide range of applications. | Dongao Li Guobo Zhang Jie Zhao Yanting Hu Yu Lu Hao Zhang Qianni Li Dongze Zhang Rong Sha Fuqiu Shao Zhengming Sheng Tongpu Yu | 2023 | High Power Laser Science and Engineering2023，11，5： | 0 |
| 8 | S19W,T27W,and N33OY mutations in ACE2 enhance SARS-CoV-2 S-RBD binding toward both wild-type and antibody-resistant viruses and its molecular basis显示文摘SARS-CoV-2 recognizes,via its spike receptor-binding domain(S-RBD),human angiotensin-converting enzyme 2(ACE2)to initiate infection.Ecto-domain protein of ACE2 can therefore function as a decoy.Here we show that mutations of S19W,T27W,and N330Y in ACE2 could individually enhance SARS-CoV-2 S-RBD binding.Y330 could be synergistically combined with either W19 or W27,whereas W19 and W27 are mutually unbeneficial.The structures of SARS-CoV-2S-RBD bound to the ACE2 mutants reveal that the enhan ced binding is mainly con tributed by the van der Waals interactio ns mediated by the aromatic side-chai ns from W19,W27,and Y330.While Y330 and W19/W27 are distantly located and devoid of any steric interference,W19 and W27 are shown to orient their side-chains toward each other and to cause steric conflicts,explai ning their in compatibility.Finally,using pseudotyped SARS-CoV-2 viruses,we dem on strate that these residue substitutions are associated with dramatically improved entry-inhibition efficacy toward both wild-type and antibody-resistant viruses.Taken together,our biochemical and structural data have delineated the basis for the elevated S-RBD binding associated with S19W,T27W,and N330Y mutations in ACE2,paving the way for potential application of these mutants in dinical treatment of COVID-19. | Fei Ye Xi Lin Zimin Che Fanli Yang Sheng Lin Jing Yang Hua Chen Honglu Sun Lingling Wang Ao Wen Xindan Zhang Yushan Dai Yu Cao Jingyun Yang Guobo Shen Li Yang Jiong Li Zhenling Wang Wei Wang Xiawei Wei Guangwen Lu | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 0 |
| 9 | Three-dimensionally ordered macroporous(3DOM) structure promoted the activity and H_(2)O poisoning resistance of Ce Mn/3DOM-TiO_(2) catalyst in NH_(3)-SCR显示文摘NH_(3)-SCR is an effective mean of NOxremoval in the non-electric industry, however, the high activation temperature and poor H_(2)O resistance of SCR catalysts posed a barrier to its application. In this work, a series of three-dimensionally ordered macroporous(3DOM) catalysts were synthesized via a colloidal crystal template(CCT) method, and various characterizations were carried out to explore the physicochemical property of catalysts. The experiment results reveal that Ce_(0.2)Mn_(0.2)/3DOM-TiO_(2) catalyst presents the excellent low-temperature catalytic activity of nearly 100% at 100℃. Furthermore, the enhanced H_(2)O resistance is achieved, certified by the unaffected NO remove at 150℃ in the participation of 15 vol% H_(2)O. The characterizations results exhibit that the improved dispersion of the active component and enhanced redox ability are conducive to the low-temperature catalytic activity. N_(2) adsorption and desorption experiments indicate that catalyst with 3DOM support possesses a larger pore diameter and specific surface area, which may weaken the condensation of H_(2)O in the microporosity of catalysts and improved the H_(2)O resistance of the catalyst. In situ DRIFTS results manifest that Ce_(0.2)Mn_(0.2)/3DOM-TiO_(2) catalyst could not only absorb more NH_(3) and generate more surface-active sites, but inhibit the competitive adsorption between H_(2)O and SCR reactants. | Yaqin Hu Shipeng Ding Chenchen Tan Peng Wu Guobo Li Sheng Wang Hongqiang Yang Yaping Zhang | 2024 | Journal of Rare Earths2024，42，3： | 0 |
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