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| 1 | Purifi cation, characterization and hypoglycemic activity of glycoproteins obtained from pea (Pisum sativum L.)显示文摘This study aimed to isolate and characterize the structures of glycoproteins from peas and determine their hypoglycemic activity.The crude pea glycoproteins(PGP)were extracted by hot water and purified by diethylaminoethyl(DEAE)-Sepharose chromatography and Sephadex G-100 size-exclusion chromatography in sequence.Then three main fractions were obtained,namely PGP1,PGP2 and PGP3,with molecular weights of 897615,846740 and 1194692 Da,respectively.The physical and chemical properties of the three fractions were evaluated and compared by Fourier transform infrared spectroscopy(FT-IR),nuclear magnetic resonance(NMR),scanning electron microscope(SEM),high performance liquid chromatography(HPLC)and other analytical techniques.The fraction PGP2 with the highest hypoglycemic activity,was screened using the Caco-2 monolayer cell model.It can inhibit the uptake of glucose in the small intestine,as well as the activities of maltase and sucrase.After simulated gastrointestinal digestion,PGP2 signifi cantly enhanced the inhibitory effect of α-glucosidase,and slightly reduced the inhibitory ability ofα-amylase.In summary,PGP2 possessed strong hypoglycemic activity after digestion.These results indicated that PGP2 has the potential to be developed into a functional food or natural medicine for the treatment of type 2 diabetes mellitus. | Gaoyixin Qin Wu Xu Junping Liu Liyan Zhao Guitang Chen | 2021 | Food Science and Human Wellness2021，10，3： | 8 |
| 2 | Ginger polysaccharide UGP1 suppressed human colon cancer growth via p53,Bax/Bcl-2,caspase-3 pathways and immunomodulation显示文摘In previous study,we got a purified ginger polysaccharide UGP1 and verified its significant antitumor activities on colon cancer HCT116 cells.In this article,we aimed to illustrate the underlying mechanism of UGP1 exerted antitumor activities on colon cancer by using in vitro cell models and in vivo animal models.The results demonstrated that UGP1 could induce S-phase cell cycle arrest,up-regulate the expression of Bax and p53,down-regulate the expression of Bcl-2,and activate the downstream protein caspase-9 and caspase-3,which was related to intrinsic apoptosis pathway on HCT116 cells.Moreover,UGP1 significantly stimulated RAW264.7 cell proliferation and secretion activity.Similarly,UGP1 inhibited tumor proliferation on tumor-bearing mice,increased the expression of p53 and the ratio of Bax/Bcl-2,enhanced the secretion of pro-inflammatory cytokines TNF-α,IL-2,IL-6 and decreased the secretion of pro-tumor cytokines TGF-βand b FGF in serum.In conclusion,it indicated that the UGP 1 could sup press human colon cancer growth by inducing apoptosis via the regulation of p53,caspase-3,and Bax/Bcl-2 ratio-dependent pathway and regulating immune system activity.Thi s investigation provided basic theoretical mechanism of ginger polysaccharideexerted antitumor activities,and contributed to develop a possible functional food or adjuvant agent for prevention or treatment of colon cancer. | Yanfang Qian Chenying Shi Chen Cheng Dengwei Liao Junping Liu Guitang Chen | 2023 | Food Science and Human Wellness2023，12，2： | 4 |
| 3 | Extraction,purification,characterization and antititumor activity of polysaccharides from Ganoderma lucidum显示文摘 | Liyan Zhao Yanhong Dong Guitang Chen | 2010 | Carbohydrate Polymers2010，80，： | 1 |
| 4 | Extraction, purification, characterization and antimmor activity of polysaccharides from Ganoderma luc/dum显示文摘 | ZHAO Liyan DONG Yanhong CHEN Guitang | 2010 | Carbohydrate Polymers2010，80，3： | 1 |
| 5 | In vitro study on antioxidant activities of peanut protein hydroly- sate显示文摘 | Chen Guitang Zhao Lin Zhao Liyan | 2007 | J Sci Food Agr2007，87，2： | 1 |
| 6 | Effect of nanocomposite-based packaging on preservation quality of green tea during one-year ambient storage显示文摘 | ZHAO Liyan LI Fang CHEN Guitang | 2012 | International Journal of Food Science and Technology2012，47，3： | 1 |
| 7 | Extraction, purification, characterization and antitumor activity of polysaccharides from Ganoderma lucidurn显示文摘 | ZHAO Liyan DONG Yanhong CHEN Guitang | 2010 | Carbohydrate Polymers2010，80，3： | 1 |
| 8 | Extraction, purification, characterization and antitumor activity of polysaccharides from Ganoderma lucidum显示文摘 | Zhao Liyan Dong Yanhong Chen Guitang | 2010 | Carbohydrate Polymers2010，80，： | 1 |
| 9 | Extraction, purification, characterization and antitumor activity of polysaccharides from Ganoderma lucidum显示文摘 | Liyan Zhao Yanhong Dong Guitang Chen Qiuhui Hu | 2009 | Carbohydrate Polymers2009，，3： | 1 |
| 10 | Extraction, purification ,characterization and antitumor activity of polysaccharides from Ganoderma lucidum 显示文摘 | Liyan Zhao Yanhong Dong Guitang Chen and Qiuhui Hu | 2010 | Carbohydrate Polymers2010，80，： | 1 |
| 11 | in vitro study on antioxidant activities of peanut protein hydrolysate显示文摘 | CHEN Guitang ZHAO Lin ZHAO Liyan | 2007 | Journal of the Science of Food and Agriculture2007，87，2： | 1 |
| 12 | In vitro study on antioxidant activities of peanut protein hydrolysate显示文摘 | CHEN Guitang ZHAO Liyan ZHAO Lin | 2007 | Journal of the Science of Food and Agriculture2007，87，2： | 1 |
| 13 | Extraction, purification, characterization and antitumor activity of polysaccharides from ganoderma iucidum 显示文摘 | Liyan Zhao Yanhong Dong Guitang Chen | 2010 | Carbohydrate Polymers2010，80，： | 1 |
| 14 | in vitro study on antioxidant activities of peanut protein hydrolysate显示文摘 | CHEN Guitang ZHAO Lin ZHAO Liyan | 2007 | Journal of the Science of Food and Agriculture2007，87，2： | 1 |
| 15 | Extraction, purification, characterization and antitumor activity of polysaccharides from Ganoderma lucidum显示文摘 | ZHAO Liyan DONG Yanhong CHEN Guitang | 2010 | Carbohydrate Polymers2010，80，3： | 1 |
| 16 | Extraction, purification, characterization and antitumor activity of polysaccharides from Ganoderma lucidum显示文摘 | ZHAO Liyan DONG Yanhong CHEN Guitang | 2010 | Carbohydrate Polymers2010，80，3： | 1 |
| 17 | Flammulina velutipes polysaccharide-iron (Ⅲ) complex used to treat iron deficiency anemia after being absorbed via GLUT2 and SGLT1 transporters显示文摘Iron deficiency anemia(IDA)is a common nutritional problem, but traditional iron supplements cause many adverse reactions. Thus, the development of a novel iron supplement might be significant for the treatment of IDA. This study aimed to study the transport mechanism of Flammulina velutipes polysaccharide-iron complex(FVP1-Fe(Ⅲ))in Caco-2 cells and the therapeutic effect on IDA rats, as well as the influence on gut microbiota in vivo. These results showed that in vitro, the uptake of FVP1-Fe(Ⅲ)was mediated by sodium-dependent glucose transporter-1(SGLT1)and facilitated glucose transporter-2(GLUT2)and GLUT2 played a dominant function. The multidrug resistance-associated protein-2(MRP-2)was involved in the efflux of FVP1-Fe(Ⅲ)across the Caco-2 cells. In vivo, FVP1-Fe(Ⅲ)had a better restorative effect on blood parameters and iron status indicators in rats with IDA as compared with FeSO_4 and exerted this effect by downregulating the expression of hepcidin. FVP1-Fe(Ⅲ)could also regulate gut microbiota dysbiosis in iron deficiency rats by returning the relative abundance of gut microbiota to the normal level. Besides, as a dietary factor, vitamin C(vit C)could enhance the therapeutic effect of FVP1-Fe(Ⅲ). These present findings showed that FVP1-Fe(Ⅲ)could be exploited as a novel iron supplement to treat IDA. | Chenying Shi Chen Cheng Xiaotong Lin Yanfang Qian Yufeng Du Guitang Chen | 2023 | Food Science and Human Wellness2023，12，5： | 0 |
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