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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Effects of aminoguanidine on nitric oxide production induced by inflammatory cytokines and endotoxin in cultured rat hepatocytes显示文摘AIM To study the effects of aminoguanidine(AG) and two L-arginine analogues Nω-nitro-L-arginine methyl ester (L-NAME) and Nω-nitro-L-arginine (L-NNA) on nitric oxide (NO) productioninduced by cytokines (TNF-α, IL-11β, and IFN-γ)and bacterial lipopolysaccharide (LPS) mixture(CM) in the cultured rat hepatocytes, andexamine their mechanisms action.METHODS Rat hepatocytes were incubatedwith AG, L-NAME, L-NNA, Actinomycin D (ActD)and dexamethasene in a medium containing CM(LPS plus TNF-α, IL-1β, and IFN-γ) for 24 h. NOproduction in the cultured supernatant wasmeasured with the Griese reaction. IntracellularcGMP level was detected with radioimmunoasey.RESULTS NO production was markedlyblocked by AG and L-NAME in a dose-dependentmanner under inflammatory stimuli conditiontriggered by CM in vitro. The rate of themaximum inhibitory effects of L-NAME (38.9%)was less potent than that obtained with AG(53.7%, P<0.05). There was no significantdifference between the inhibitory effects of AGand two L-arginine analogues on intracellularcGMP accumulation in rat cultured hepatocytes.Non-specific NOS expression inhibitordexamethasone ( DEX ) and iNOS mRNAtranscriptional inhibitor ActD also significantlyinhibited CM-induced NO production. AG(0.1mmol.L-1) and ActD (0.2ng@Lt) wereequipotent in decreasing NO production inducedby inflammatory stimuli in vitro, and botheffects were more potent than that induced bynon-selectivity NOS activity inhibitor L-NAME(0. 1 mmol@ L- 1) under similar stimuli conditions(P | Guo Liang Zhang Ye Hong Wang Hui Ling Teng Zhi Bin Lin Department of Pharmacology,School of Basic Medical Sciences,Beijing University,Beijiog 100083,ChinaDr.Guo Liang Zhang graduated from Xinxiang Medical College in 1982,got Ph.D.at Nagoya City University Medical School,Japan in 1994,finished postdoctoral research at Beijing Medical Univcrsity in 1996,now an associate professor of pharmacology,specialized in hepatic pharmacology,having 15 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 20 |
| 3 | Hongda New Material Improved Silicone Rubber Industrial Chain显示文摘 | Jiangsu Hongda Chemical New Material Co.,Ltd.(Hongda New material,SZ:002211)is located in Yangzhong,Jiangsu province.It got listed in Shenzhen Stock Exchange onFebruary 1st,2008.(CCR2008, No.3)The company mainly produces silicone monomersand high-temperature silicone rubber series.The capacity of silicone monomers and prod-ucts of silicone rubber series reached 30 000 t/a and 28 000 t/a respectively at the end of2007.The sales volume of high-temperature silicone rubber compound has grown steadilyat 30% a year.The company’s high-temperature silicone rubber capacity is still beingexpanded today and will hopefully reach 50 000 t/a at the end of 2008. | 2008 | China Chemical Reporter2008，，25： | 16 |
| 4 | Transretinoic acid inhibits rats gastric epithelial dysplasia induced by N-methyi-N-nitro-N-nitrosoguanidine:influences on cell apoptosis and expression of its regulatory genes显示文摘INTRODUCTIONGastric epithelial dysplasia (GED) hypothetically is a straight-forward concept: dysplastic epithelium replacing the normal gastric epithelium of the stomach [1].In the stomach ,like any other segment of the gut ,it is defined as an unequivocal non-invasive epithelial change[2,3].The observation of gastric dysplasia as a cancerous lesion was recognized over a century ago ,but it is only after the advent of gastroscopy that its clinical significance has been stressed[4-7]. | Ru Tao Cui~1 Gan Cai~2 Zhao Bao Yin~(2*) Yong Cheng~2 Qiu Hong Yang~1 Tao Tian~(3#) ~1Liver Center,Beijing Friendship Hospital Affiliated to CapitalUniversity of Medical Sciences~2Department of Gastroenterology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai China and Department of Pathophysiology.Shanghai Medical University.Shanghai,China ~3Pathological Department,Shanghai Medical University (?)Now working at UCLA,USA ~#Now working at University of Michigan,USADr.Ru Tao Cui graduated and got the bachelor degree from Shandong University of Traditional Chinese Medicine in 1994,got doctor degree from Shanghai University of Traditional Chinese Medicine in 2000,now studying as a postdoc,majoring gastroenterology,having 29 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 8 |
| 5 | Contrast-enhanced endoscopic ultrasonography in digestive diseases显示文摘 | Yoshiki Hirooka Akihiro Itoh Hiroki Kawashima Eizaburo Ohno Yuya Itoh Yosuke Nakamura Takeshi Hiramatsu Hiroyuki Sugimoto Hajime Sumi Daijiro Hayashi Naoki Ohmiya Ryoji Miyahara Masanao Nakamura Kohei Funasaka Masatoshi Ishigami Yoshiaki Katano Hidemi Got | 2012 | Journal of Gastroenterology2012，，10： | 2 |
| 6 | Contrast-enhanced endoscopic ultrasonography in digestive diseases显示文摘 | Yoshiki Hirooka Akihiro Itoh Hiroki Kawashima Eizaburo Ohno Yuya Itoh Yosuke Nakamura Takeshi Hiramatsu Hiroyuki Sugimoto Hajime Sumi Daijiro Hayashi Naoki Ohmiya Ryoji Miyahara Masanao Nakamura Kohei Funasaka Masatoshi Ishigami Yoshiaki Katano Hidemi Got | 2012 | Journal of Gastroenterology2012，，10： | 2 |
| 7 | Viability and virulence of experimentally stressed noncuhurable Salmonella typhimurium 显示文摘 | Caro A Got P Lesne J Binard S | 1999 | Appl Environ Microbiol1999，65，7： | 1 |
| 8 | Respective roles ofculturable and viable-but-nonculturable cells in the heteroge-neity of Salmonella enterica serovar Typhimurium invasive-ness显示文摘 | PASSERAT J GOT P DUKAN S | 2009 | Appl Environ Microbiol2009，75，16： | 1 |
| 9 | Judging the intensity of facial expres?sions of emotion: Depression-related biases in the processing of positive affect显示文摘 | Yoon KL JoormannJ Got!ib IH | 2009 | J Abnorm Psychol2009，118，1： | 1 |
| 10 | Oculographicfindings in 145 patients with benign paroxysmal positional vertigo显示文摘 | Domenech Campos E Armen Got Carcell ER M Barona DE | 2006 | Acta Otorrinola ringol Esp2006，57，： | 1 |
| 11 | Mechanical Behaviour of a Fuel Cell Stack Under Vibrating Conditions Linked to Aircraft Applications Part h Experimental显示文摘 | ROUSS V LESAGE P BI~GOT S | 2008 | International Journal of Hydrogen Energy2008，，33： | 1 |
| 12 | Biomechanical compari- son of parallel versus 90 - 90 plating of bicolumn distal hu-merus fractures with intra - articular comminution 显示文摘 | Got C Shuck J Biereevicz A | 2012 | J Hand Surg(Am)2012，37，12： | 1 |
| 13 | Biomechanical Comparision of parallel versus 90-90plating of bicolumn distal humerus fracture with intra-articular comminution显示文摘 | Got C Shuck J Biercevicz A | 2012 | J Hand Surg(Am)2012，37，12： | 1 |
| 14 | Wrought Ni-base superalloys for steam turbine applications beyond 700~C 显示文摘 | Roster J Gotting M Del Genovese D | 2005 | Advanced Engineering Materials2005，，5： | 1 |
| 15 | Management of patients hospitalized for diabetic foot infection: Results of the French OPIDIA study显示文摘 | J.-L. Richard J.-P. Lavigne I. Got A. Hartemann D. Malgrange D. Tsirtsikolou A. Baleydier E. Senneville | 2010 | Diabetes and Metabolism2010，，3： | 1 |
| 16 | Prevalence of Act inobacillus pleuropneumoniae,Actinobacillus suis,Haemophiluspara suis,Pasteurella multocida,and Streptococcus suis in representative Ontario swine herds显示文摘 | MACINNES J GOT T SCHALK M LONE A G | 2008 | Can J Vet Res2008，72，3： | 1 |
| 17 | Cancer stem cell marker in eirculating tumor cells: expression of CD44 variant exon 9 is strongly correlated to treatment refractoriness, recurrence and prognosis of human colorectal cancer 显示文摘 | Katoh S Got T Naruse T | 2015 | Anticancer Res2015，35，1： | 1 |
| 18 | UHPLC determination of catechins for the quality control of green tea显示文摘 | Naldi M Fiori J Got i R | | 0，，： | 1 |
| 19 | Human xylosyltransferases in health and disease显示文摘 | Gotting C Kuhn J Kleesiek K | 2007 | Cell Mol Life Sci2007，64，12： | 1 |
| 20 | Viability and virulence ofexperimentally stressed nonculturable Salmonella typhimu-rium显示文摘 | CARO A GOT P LESNE J | 1999 | Appl Environ Microbiol1999，65，7： | 1 |
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