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| 1 | Absolute copy number differences of Y chromosomal genes between crossbred (Bos taurus×Bos indicus) and Indicine bulls显示文摘Background: The Y chromosome in mammal is paternally inherited and harbors genes related to male fertility and spermatogenesis. The unique intra-chromosomal recombination pattern of Y chromosome and morphological difference of this chromosome between Bos taurus and Bos indicus make it an ideal model for studying structural variation, especially in crossbred(Bos taurus × Bos indicus) bulls. Copy Number Variation(CNV) is a type of genomic structural variation that gives information complementary to SNP data. The purpose of this study was to find out copy number differences of four Y chromosomal spermatogenesis-related candidate genes in genomic DNA of crossbred and purebred Indicine bulls. Result: Four Y chromosomal candidate genes of spermatogenesis namely, sex determining gene on Y chromosome(SRY), DEAD box polypeptide 3-Y chromosome(DDX3Y), Ubiquitin specific peptidase 9, Y-linked(USP9Y), testis-specific protein on Y chromosome(TSPY) were evaluated. Absolute copy numbers of Y chromosomal genes were determined by standard curve-based quantitative real time PCR. Copy numbers of SRY and TSPY genes per unit amount of genomic DNA are higher in crossbred than Indicine bulls. However, no difference was observed in DDX3Y and USP9Y gene copy numbers between two groups. Conclusion: The present study demonstrates that the structural organization of Y chromosomes differs between crossbred and Indicine bulls which are reproductively healthy as observed from analysis of semen attributes. The absolute copy numbers of SRY and TSPY genes in unit mass of genomic DNA of crossbred bulls are significantly higher than Indicine bulls. No alteration in absolute copies of DDX3Y and USP9Y gene was found between the genome of crossbred and Indicine bulls. This study suggests that the DDX3Y and USP9Y are likely to be single copy genes in the genome of crossbred and Indicine bulls and variation in Y chromosome length between crossbred and Indicine bulls may be due to the copy number variation of SRY gene and TSPY array. | Ayan Mukherjee Gulshan Dass Jagan Mohanarao G Moloya Gohain Biswajit Brahma Tirtha Kumar Datta Sachinandan De | 2013 | Journal of Animal Science and Biotechnology2013，4，4： | 3 |
| 2 | Protonated dyesurfactant ion pair formation between neutral red and anionic surfactants in aqueous submicellar solutions显示文摘 | BIREN Gohain SURASHREE Sarma | 2008 | Journal of Molecular Liquids2008，，142： | 1 |
| 3 | Geosteering in Umm Gudair Field with simultaneous density and resistivity images in real time (first time in the world) 显示文摘 | Gohain A K Abdullah Al-Anzi N Ita Archibong E | 2007 | SPE/IADC107506-MS2007，，： | 1 |
| 4 | Understanding Darjeeling tea flavour on a molecular basis 显示文摘 | Gohain B Borchetia S Bhorali P | 2012 | Plant Molecular Biology2012，78，6： | 1 |
| 5 | Luminescent properties and particle morphology of Ca3(PO4)z: Gd3+, Pr3+ phosphor powder prepared by microwave assisted synthesis显示文摘 | Mokoena P P Gohain M Bezuidenhoudt B C B | 2014 | Journal of Luminescence2014，155，： | 1 |
| 6 | TOF SIMS analysis and enhanced UVB photoluminescence by energy transfer from Pr3+ to Gd3+ in Ca3(PO4)z:Gd3+,pra+ phosphor prepared by urea assisted combustion显示文摘 | Mokoena P P Gohain M Kumar V | 2014 | Journal of Alloys &Compounds2014，595，15： | 1 |
| 7 | Recent advances in the application of supercritical fluids for carbon-carbon bond formation in organic synthesis显示文摘 | Prajapati D Gohain M | 2004 | Tetrahedron2004，60，： | 1 |
| 8 | A novel Cucatalysed three-component one-pot synthesis of dihydropyrimidin-2 (1H)-ones using microwaves under solvent-free conditions显示文摘 | GOHAIN M PRAJAPATI D SANDHUB S J | 2004 | Synlett2004，，2： | 1 |
| 9 | Protonated Dye-surfactant Ion-pair Formation Between Neutral Red and Anionic Surfactants in Aqueous Submicellar Solutions显示文摘 | Gohain B Sarma S Dutta R K | 2008 | J Mol Liq2008，142，： | 1 |
| 10 | Iodine a simple,effective and inexpensive catalyst for the synthesis of substituted coumarins显示文摘 | Prajapati D Gohain M | 2007 | Catalysis Letters2007，119，12： | 1 |
| 11 | A Visible Spectro- scopic Study of Mierostructure of O/W Microcmulsions of Anionic Surfactants: Effect of Cosuffactant 显示文摘 | Sarma Surashree Gohain Biren Dutta Robink | 2006 | Journal of Dispersion Sci- ence & Technology2006，27，6： | 1 |
| 12 | A preclinical study on the gastric ulcer pro- tective activity of the world s hottest chilli, Capsicum frutescens 显示文摘 | Das S Deka S Gohain K | 2008 | J Clin Diag Res2008，2，： | 1 |
| 13 | Understanding Darjeeling tea flavor on a molecular basis显示文摘 | Gohain B Borchetia S Bhorali P | 2012 | Plant Molecular Biology2012，78，6： | 1 |
| 14 | A simple,effective and inexpensive catalyst for the synthesis of substituted coumarins显示文摘 | Prajapati D Gohain M Iodine | 2007 | Catal Lett2007，119，： | 1 |
| 15 | Effect of application of urea - N, rock phosphate and muriate of potash on suphur nutrition of tea 显示文摘 | K K Gohain and Amrit Dutta | 1994 | Two - and - a Bud1994，41，1： | 1 |
| 16 | Novel Gallium-Mediated C3-Allylation of Indoles and Pyrroles in Aqueous Media Promoted by Bu4NBr显示文摘 | Prajapati D Gohain M Gogoi B J | | 0，，21： | 1 |
| 17 | A preclinical study on the gastric ulcer protective activity of the world' s hottest chilli, Capsicum frutescenes显示文摘 | Das S Deka S Gohain K | 2008 | J Clin Diagn Res2008，，2： | 1 |
| 18 | Premicellar and micelle formation behavior of dye surfactant ion pairs in aqueous solutions:deprotonation of dye in ion pair micelles显示文摘 | BIREN Gohain ROBIN K Dutta | 2008 | Journal of Colloid and Interface Science2008，，323： | 1 |
| 19 | Preparation of phenolic compounds through catalyst-free ipso- hydroxylation of arylboronic acids 显示文摘 | GOHAIN M DU P M VAN TONDER J H | 2014 | Tetrahedron Letters2014，55，13： | 1 |
| 20 | Iodine a simple, effective and inexpensive catalyst for the synthesis of substituted coumarins 显示文摘 | Prajapati D Gohain M | 2007 | Cats Lett2007，119，： | 1 |
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