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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Toxicity of Graphene Quantum Dots in Zebrafish Embryo显示文摘Objective To evaluate the bio-safety of graphene quantum dots(GQDs), we studied its effects on the embryonic development of zebrafish. Methods In vivo, biodistribution and the developmental toxicity of GQDs were investigated in embryonic zebrafish at exposure concentrations ranging from 12.5-200 μg/m L for 4-96 h post-fertilization(hpf). The mortality, hatch rate, malformation, heart rate, GQDs uptake, spontaneous movement, and larval behavior were examined. Results The fluorescence of GQDs was mainly localized in the intestines and heart. As the exposure concentration increased, the hatch and heart rate decreased, accompanied by an increase in mortality. Exposure to a high level of GQDs(200 μg/mL) resulted in various embryonic malformations including pericardial edema, vitelline cyst, bent spine, and bent tail. The spontaneous movement significantly decreased after exposure to GQDs at concentrations of 50, 100, and 200 μg/mL. The larval behavior testing(visible light test) showed that the total swimming distance and speed decreased dose-dependently. Embryos exposed to 12.5 μg/mL showed hyperactivity while exposure to higher concentrations(25, 50, 100, and 200 μg/mL) caused remarkable hypoactivity in the light-dark test. Conclusion Low concentrations of GQDs were relatively non-toxic. However, GQDs disrupt the progression of embryonic development at concentrations exceeding 50 μg/mL. | WANG Zhen Guo ZHOU Rong JIANG Dan SONG Jing E XU Qian SI Jing CHEN Yun Ping ZHOU Xin GAN Lu LI Jian Zhen ZHANG Hong LIU Bin | 2015 | Biomedical and Environmental Sciences2015，28，5： | 6 |
| 3 | Effect of tungsten oxide on ceria nanorods to support copper species as CO oxidation catalysts显示文摘In this work,tungsten oxide with different concentrations(0,0.4 at%,2.0 at%and 3.2 at%)was introduced to the ceria nanorods via a deposition-precipitation(DP)approach,and copper species of ca.10 at%were sequentially anchored onto the modified ceria support by a similar DP route.The aim of the study was to investigate the effect of the amount of tungsten oxide(0,0.4 at%,2.0 at%,and 3.2 at%)modifier on the copper-ceria catalysts for CO oxidation reaction and shed light on the structure-activity relationship.By the aids of multiple characterization techniques including N2 adsorption,high-resolution transmission electron microscopy(HRTEM),powder X-ray diffraction(XRD),X-ray absorption fine structure(XAFS),and temperature-programmed reduction by hydrogen(H2-TPR)in combination with the catalytic performance for CO oxidation reaction,it is found that the copper-ceria samples maintain the crystal structure of the fluorite fcc CeO2 phase with the same nanorod-like morphology with the introduction of tungsten oxide,while the textural properties(the surface area,pore volume and pore size)of ceria support and copper-ceria catalysts are changed,and the oxidation states of copper and tungsten are kept the same as Cu2+and W6+before and after the reaction,but the introduction of tungsten oxide(WO3)significantly changes the metal-support interaction(transfer the CuOx clusters to Cu-[Ox]-Ce species),which delivers to impair the catalytic activity of copper-ceria catalysts for CO oxidation reaction. | Yu'nan Li Lin Gan Rui Si | 2021 | Journal of Rare Earths2021，39，1： | 5 |
| 4 | Protective Effects of Shikonin on Brain Injury Induced by Carbon Ion Beam Irradiation in Mice显示文摘Radiation encephalopathy is the main complication of cranial radiotherapy.It can cause necrosis of brain tissue and cognitive dysfunction.Our previous work had proved that a natural antioxidant shikonin possessed protective effect on cerebral ischemic injury.Here we investigated the effects of shikonin on carbon ion beam induced radiation brain injury in mice.Pretreatment | GAN Lu WANG Zhen Hua ZHANG Hong ZHOU Rong SUN Chao LIU Yang SI Jing LIU Yuan Yuan WANG Zhen Guo | 2015 | Biomedical and Environmental Sciences2015，28，2： | 5 |
| 5 | Endothelium-independent vasorelaxant effect of 20(S)-protopanaxadiol on isolated rat thoracic aorta显示文摘人参皂甙被认为是主要 pharmacologically 活跃的人参成分,而 20 (S)-protopanaxadiol [20 (S)-PPD] 是在内脏的人参皂甙的活跃代谢物。在这研究,我们在孤立的老鼠上调查了 20 (S)-PPD 的效果象它的 vasorelaxant mechanisms.Methods 一样的胸的主动脉:大动脉的戒指与或没有内皮细胞层从 Wistar 老鼠被准备并且在器官房间推迟了。在准备的紧张的变化通过连接到一个数据获得系统的等轴的变换器被记录。大动脉的戒指是有 phenylephrine 的 precontracted (PE, 1 mol/L ) 或 high-K +(80 mmol/L ).Results:20 (S)-PPD (21.5-108.5 mol/L ) 的申请与分别地导致了 90.4 或 46.5 mol/L 的 EC 50 价值的 PE 或 high-K +, 引起了集中依赖者内皮细胞层未经触动的大动脉的戒指 precontracted 的 vasodilation。内皮细胞层的移动没在 20 (S)-PPD-induced 松驰上有效果。 20 (S) -PPD 的 vasorelaxant 效果没被 preincubation 也与肾上腺素能的受体对手 propranolol ,或与ATP敏感的 K +隧道 blocker glibenclamide 影响,电压依赖者 K +隧道 blocker 4-AP 和里面的整流器 K +隧道 blocker BaCl 2,而它被 preincubation 显著地与 Ca 2+-activated K +稀释(放射性元素 Ca )隧道 blocker 茶( 1 mmol/L )。而且,没有合成, cGMP 和 prostacyclin 小径的抑制没影响 20 (S)-PPD 的 vasorelaxant 效果。在 Ca 2+-free 答案, 20 (S)-PPD (108.5 mol/L ) 显著地减少了在有 PE 或 high-K + 的大动脉的戒指 precontracted 的细胞外的 Ca 2+-induced 收缩和减少的导致 PE 的短暂收缩。电压依赖者 Ca 2+ 隧道对手 nifedipine 禁止了导致 PE 的收缩;进一步的抑制在操作受体的 Ca 2+ 隧道禁止者 SK&F 96365 或 20 (S)-PPD.Conclusion 的申请以后被观察:20 (S)-PPD 经由一条内皮细胞层无关的小径导致 vasorelaxation。电压依赖者 Ca 2+ 隧道和操作受体的 Ca 2+ 隧道和 Ca 2+-activated K + 隧道的激活的抑制可能涉及松驰。 | Lu GAN Zhen-hua WANG Hong ZHANG Xin ZHOU Hui ZHOU Chao SUN Jing SI Rong ZHOU Cheng-jun MA Ji LI | 2016 | Acta Pharmacologica Sinica2016，37，12： | 5 |
| 6 | Experimental Study on Analgesia and Hemostasis of Paris polyphylla,Scutellaria baicalensis and Their Compatibility显示文摘[Objectives]The analgesic and hemostatic effects of Paris polyphylla,Scutellaria baicalensis,and their compatibility were studied.[Methods](i)In the experiment on the writhing of mice induced by acetic acid,60 Kunming SPF mice were randomly selected,and were randomly divided into 6 groups according to the male-to-female ratio of 1∶1,including blank control group,P.polyphylla group,S.baicalensis group,and three P.polyphylla and S.baicalensis compatibility groups(with the ratio of 1∶2,1∶1 and 1∶2).There were 10 mice in each group.In the experimental groups,the mice were given 1 g/mL Chinese medicine extract according to a dose of 20 mL/kg.In the control group,the mice were given 0.9%normal saline in equal volumes.Gavage was performed one time every 24 h and lasted for 14 d.1 h after the gavage on the 14th day,they were given 0.5%glacial acetic acid solution via intraperitoneal injection.(ii)In the hot plate experiment,60 Kunming SPF mice were selected,and the ratio of male to female,grouping,administration and gavage were the same as those of the glacial acetic acid-induced writhing experiment.Gavage was lasted for 14 d.1 h after the gavage on the 14th day,the mice were placed on a hot plate apparatus at(55±0.5)℃,and the time of licking hindfoot was measured.(iii)The hemostatic effect was explored through three experimental methods of tail hemostasis,femoral artery hemostasis and liver hemostasis.60 Kunming SPF mice were taken in each of the three experiments,and the male-female ratio and grouping were the same as above.The powder was covered on the surface of the wound sites,and the bleeding was observed.The hemostatic time was recorded,and the hemostatic time was recorded as 3 min if it exceeded 3 min.[Results](i)In the acetic acid-induced writhing experiment,compared with the blank control group,the pain of mice in the experimental groups was inhibited.Among them,P.polyphylla group,S.baicalensis group,compatibility 1∶2 group,compatibility 1∶1 group,and compatibility 2∶1 group had significant effects(P<0.05),and the inhibition rate of writhing was 20.43%,28.32%,52.30%,32.79%,and 39.02%,respectively.The analgesic effect of the compatibility 1∶2 group was the most obvious.(ii)In the hot plate experiment,compared with the blank control group,the experimental groups had analgesic effect.Among them,P.polyphylla group,S.baicalensis group,compatibility 1∶2 group,compatibility 1∶1 group,and compatibility 2∶1 group had significant effects(P<0.05),and the increase rate of pain threshold was 29.17%,47.83%,61.54%,and 50.61%,and 53.83%,respectively.The analgesic effect of the compatibility 2∶1 group was the most significant.(iii)In the hemostatic experiment,there was a significant difference in hemostatic time between the compatibility 1∶2 group and the blank control group,P.polyphylla group,and S.baicalensis group(P<0.05).The hemostatic effect of the compatibility 1∶2 group was the best.[Conclusions]P.polyphylla,S.baicalensis,and its compatibility had good analgesic and hemostatic effects,and the best compatibility ratio was 1∶2. | Shibo ZHAO Suoyi HUANG Zhenjing SI Lanlan GAN Shusheng HUANG Ye FU Jianjie LAN Xueying YU Yuekong CEN | 2020 | Medicinal Plant2020，11，2： | 2 |
| 7 | Lamivudine for the treatment of membranous glomerulopathy secondary to chronic Hepatitis B infection 显示文摘 | Gan SI Devlin SM Scott-Douglas NW | 2005 | Can J Gastroenterol2005，19，10： | 1 |
| 8 | Ridge-furrow and plastic-mulching tillage enhances maize–soil interactions: Opportunities and challenges in a semiarid agroecosystem显示文摘 | Li-Min Zhou Sheng-Li Jin Chang-An Liu You-Cai Xiong Jian-Ting Si Xiao-Gang Li Yan-Tai Gan Feng-Min Li | 2011 | Field Crops Research2011，，： | 1 |
| 9 | Admission hematoerit: a simple,useful and early predictor of severe pancreatitis 显示文摘 | Gan SI Romagnuolo J | 2004 | Dig Dis Sci2004，49，1112： | 1 |
| 10 | Dissipativity of 0-methods for nonlinear volterra delay-integru-differential equations 显示文摘 | GAN SI Q1NG | 2007 | J Comput AppI Math2007，206，2： | 1 |
| 11 | Role of EUS显示文摘 | ASGE Standards of Practice Committee Gan SI Raian E etal | 2007 | Gastrointest Endosc2007，366，3： | 1 |
| 12 | Research on the influence of the differ- ent logistics demand structures of the city in regional logistics plan- ning显示文摘 | Si Chen Gan Mi Dingqi Shuai | 2014 | Computer Modeling&New Technologies2014，18，4： | 1 |
| 13 | Comparison of micro-CT and cone beam CT-based assessments for relative difference ofgrey level distribution in a human mandible 显示文摘 | Taylor TT Gans SI Jones EM | 2012 | Dentomaxillo- fac Radiol2012，9，20： | 1 |
| 14 | Role of EU S显示文摘 | ASGE Standards of Practice Committee Gan SI Rajan E | 2007 | GastrointestEndosc2007，66，3： | 1 |
| 15 | Effects of Exogenous Carbon Monoxide Releasing Molecules on the Development of Zebrafish Embryos and Larvae显示文摘The use of exogenous carbon monoxide releasing molecules(CORMs)provides promise for clinical application;however,the hazard potential of CORMs in vivo remains poorly understood.The developmental toxicity of CORM-3 was investigated by exposure to concentrations ranging from 6.25 to400μmol/L during 4-144 h post fertilization.Toxicity endpoints of mortality,spontaneous movement,heart rate,hatching rate,malformation,body length,and larval behavior were measured. | SONG Jing E SI Jing ZHOU Rong LIU Hua Peng WANG Zhen Guo GAN Lu GUI Fang LIU Bin ZHANG Hong | 2016 | Biomedical and Environmental Sciences2016，29，6： | 1 |
| 16 | Role of EUS显示文摘 | Gan SI Rajah E Adler DG | 2007 | Gastrointestinal Endoscopy2007，66，： | 1 |
| 17 | Hypertriglycer-idemia-induced pancteatitis:a case-based review显示文摘 | Gan SI Edwards AL Symonds C J | | 0，，07： | 1 |
| 18 | Lamivudine for the treatment of membranous glomerulopathy secondary to chronic Hepatitis B infection显示文摘 | Gan SI Devlin SM Scott-Douglas NW | 2005 | Can J Gastroenterol2005，19，10： | 1 |
| 19 | Management of antithrombotic agents for endoscopic procedures显示文摘 | Anderson MA Ben-Menachem T Gan SI | 2009 | Gastrointest Endosc2009，70，6： | 1 |
| 20 | PHISDetector:A Tool to Detect Diverse In Silico Phage–host Interaction Signals for Virome Studies显示文摘Phage-microbe interactions are appealing systems to study coevolution,and have also been increasingly emphasized due to their roles in human health,disease,and the development of novel therapeutics.Phage-microbe interactions leave diverse signals in bacterial and phage genomic sequences,defined as phage-host interaction signals(PHISs),which include clustered regularly interspaced short palindromic repeats(CRISPR)targeting,prophage,and protein-protein interaction signals.In the present study,we developed a novel tool phage-host interaction signal detector(PHISDetector)to predict phage-host interactions by detecting and integrating diverse in silico PHISs,and scoring the probability of phage-host interactions using machine learning models based on PHIS features.We evaluated the performance of PHISDetector on multiple benchmark datasets and application cases.When tested on a dataset of 758 annotated phage-host pairs,PHISDetector yields the prediction accuracies of 0.51 and 0.73 at the species and genus levels,respectively,outperforming other phage-host prediction tools.When applied to 125,842 metagenomic viral contigs(mVCs)derived from 3042 geographically diverse samples,a detection rate of 54.54% could be achieved.Furthermore,PHISDetector could predict infecting phages for 85.6% of 368 multidrug-resistant(MDR)bacteria and 30% of 454 human gut bacteria obtained from the National Institutes of Health(NIH)Human Microbiome Project(HMP).The PHISDetector can be run either as a web server(http://gffzz439de54f28c841f5h9n9w5ob0fvnu65u9.ffgz.tsg.suse.edu.cn/PHISDetector/)for general users to study individual inputs or as a stand-alone version(http://gffzz188fe103f8f1460as9n9w5ob0fvnu65u9.ffgz.tsg.suse.edu.cn/HITImmunologyLab/PHISDetector)to process massive phage contigs from virome studies. | Fengxia Zhou Rui Gan Fan Zhang Chunyan Ren Ling Yu Yu Si Zhiwei Huang | 2022 | Genomics, Proteomics & Bioinformatics2022，20，3： | 0 |
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