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| 1 | Integrated positron emission tomography and computed tomography in preoperative lymph node staging of non-small cell lung cancer显示文摘 | Xu N Jiao Y Fang WG Wang MZ Zhu ZH Zhang YQ | 2014 | Chinese Medical Journal2014，，4： | 8 |
| 2 | Neuroprotective effects of INT-777 against Aβ1-42-induced cognitive impairment, neuroinflammation, apoptosis, and synaptic dysfunction in mice显示文摘OBJECTIVE To investigate the neuroprotective effects of TGR5 agonist INT-777 in the Aβ_(1-42)-treated mouse model of acute neurotoxicity.METHODS Cognitive impairment was induced by single intracerebroventricular injection of aggregated Aβ_(1-42)(410 pmol per mouse;5 μL)into the mouse brain in normal ICR mice.After3 d,INT-777(0.75,1.5 or 3.0 μg per mouse) was infused into the same site.Another 3 d later,the cognition function was evaluated by Morris water maze(MWM),novel objective recognition(NOR),and Y maze tests.Furthermore,the levels of TGR5,TNF-α,IL-1β,IL-6,NF-κB p65,Iba1,caspase 3,Bcl-2,Bax,PSD95,and synaptophysin in the hippocampus and frontal cortex were detected using Western blotting.Microglia activation in the hippocampal CA1,CA3 and DG regions and frontal cortex were detected by immunohistochemistry.TUNEL staining was used to detect the apoptotic cells.The number of spines in the hippocampal CA1,CA3 and DG regions and frontal cortex was detected by GolgiCox staining.RESULTS INT-777(1.5 or 3.0 μg per mouse) significantly ameliorated memory impairment in the Aβ_(1-42)-treated mice,evidenced by increased the time spent in the target quadrant(P<0.05),and the number of target crossings(P<0.05) in the MWM test,increased the discrimination index in the NOR test(P<0.05),and increased the number of correct choices(P<0.05,P<0.01) and decreased the latency(P<0.05) to enter the shock-free compartment in the Y-maze test.Importantly,this treatment reversed Aβ_(1-42)-induced TGR5 down-regulation(P<0.05,P<0.01),suppressed the increase of nuclear NF-κB p65(P<0.05,P<0.01) and mitigated neuroinflammation,evidenced by lower proinflammatory cytokines such as TNF-α(P<0.05),IL-1β(P<0.05),IL-6(P<0.05,P<0.01),and less Iba1-positive cells in the hippocampal CA1,CA3 and DG regions and frontal cortex(P<0.05,P<0.01) as well as the protein level of Iba1.INT-777(1.5 or3.0 μg per mouse) also pronouncedly suppressed apoptosis through the reduction of TUNEL-positive cells(P<0.05,P<0.01),decreased activation of caspase 3(P<0.05),increased ratio of Bcl-2/Bax(P<0.05,P<0.01),and ameliorated synaptic dysfunction via promoting dendritic spines generation(P<0.05,P<0.01) with the upregulation of PSD95(P<0.05) and synaptophysin proteins(P<0.05).CONCLUSION INT-777 has potent neuroprotective effects against Aβ_(1-42)-induced neurotoxicity,which suggests that the activation of TGR5 could be a novel and promising strategy for the treatment of AD. | WU Xian LYU Yang-ge DU Yi-feng CHEN Fang Miranda N REED HU Mei Vishnu SUPPIRAMANIAM TANG Su-su HONG Hao | 2018 | 中国药理学与毒理学杂志2018，32，9： | 6 |
| 3 | Immobilized enzyme reactors in HPLC and its application in inhibitor screening:A review显示文摘This paper sets out to summarize the literatures based on immobilized enzyme bio-chromatography and its application in inhibitors screening in the last decade.In order to screen enzyme inhibitors from a mass of compounds in preliminary screening,multi-pore materials with good biocompatibility are used for the supports of immobilizing enzymes,and then the immobilized enzyme reactor applied as the immobilized enzyme stationary phase in HPLC.Therefore,a technology platform of high throughput screening is gradually established to screen the enzyme inhibitors as new anti-tumor drugs.Here,we briefly summarize the selective methods of supports,immobilization techniques,co-immobilized enzymes system and the screening model. | Si-Meng Fang a,Hai-Na Wang a,Zhong-Xi Zhao b,Wei-Hong Wang a,n a School of Pharmaceutical Sciences,Shandong University,Ji’nan,China b School of Pharmaceutical Sciences and Center for Pharmaceutical Research & Drug Delivery Systems,Shandong University,Ji’nan,China | 2012 | Journal of Pharmaceutical Analysis2012，2，2： | 3 |
| 4 | 全颗粒饲料对奶牛生产性能的影响显示文摘饲料中的中性洗涤纤维(NDF)含量和饲料颗粒直径是影响瘤胃功能的重要因素。试验评估了以颗粒饲料为主的奶牛瘤胃健康情况、NDF消化率和生产性能。选取8头荷斯坦母牛(336±30 d,体重346±35 kg),随机分配到交叉重复试验组中,奶牛安置于牛栏,自由采食。试验期为3周,前两周是适应期,最后一周为数据收集期。各组饲粮原料相同,但物理形式不同,分为全混合日粮(TMR)和颗粒料(直径=8 mm)。两组间物理有效NDF(pe NDF)不同,TMR组为39.8%,颗粒料组为11.8%。在试验过程中,干物质采食量(DMI)、饮水量、瘤胃反刍时间、瘤胃温度和pH情况均记录。3周后收集粪样,确定潜在的可消化NDF总消化率(pdNDF)。每阶段结束后计算平均日增重和饲料转化率。颗粒料组的DMI、DMI/体重和饮水量更高。而两组间平均日增重和饲料转化率无显著差异。颗粒料组的瘤胃反刍时间为241 min·d-1低于TMR组的507 min·d-1。饲料的不同物理外形对瘤胃温度、瘤胃pH无显著影响。TMR组的pdNDF总消化率为90.25%高于颗粒料组的86.82%。试验结果表明,动物易接受完整的颗粒饲料,DMI较高,且反刍时间减少,但瘤胃pH无显著变化。TMR组的pdNDF消化率显著增加。尽管两组奶牛在DMI和纤维消化方面表现不同,但总体表现相似,两组饲料的保存时间与其物理形式有关。在短期内,提供能满足NDF需求的完全颗粒饲料可改善瘤胃健康状况和动物生产性能,而较长时间的数据则需要进一步试验。 | Wang J Yang M Cao M Lin Y Che L Q Duraipandiyan V Al-Dhabi N A Fang Z F Xu S Y Feng B Liu G Wu D Bonfante E Palmonari A Mammi L Canestrari G Fustini M Formigoni A | 2016 | 饲料博览2016，0，12： | 2 |
| 5 | Synthesis and structural characterization of several tris(2-methyl-2-phenylpropyl)tin carboxylates contanining germanium显示文摘 | Fang X N Song X Q Xie Q L | | 0，，： | 2 |
| 6 | Taxuchin B,a New Chlorine-Containing Taxoid显示文摘A new taxoid, taxuchin B[1], has been isolated from twigs and needles of Taxus chinensis. It is the first chlorine-containing taxoid reported to | Fang, WS Fang, QC Liang, XT Lu, Y Wu, N Zheng, QT | 1997 | Chinese Chemical Letters1997，8，3： | 2 |
| 7 | Epoxyeicosatrienoic acids increase intracellular calcium concentration in vascular smooth muscle cells显示文摘 | Fang X Weintraub N L Stoll L L Spector A A | 1999 | Hypertension1999，34，6： | 1 |
| 8 | Discovery of a nanomolar inhibitor of the human murinc double minute 2 (MDM2)-p53 interaction through an integrated,virtual database screening strategy显示文摘 | LU Y ZANETA N C FANG X | 2006 | J Med Chem2006，49，13： | 1 |
| 9 | A new system of variational inclusions with (H,r/) -monotone operators in Hilbert spaces显示文摘 | Fang Y P Huang N J Thompson H B | 2005 | Comput Math Appl2005，49，： | 1 |
| 10 | Formation of fine near field scanning optical microscopy tips-part Ⅰ:by static and dynamic chemical etching显示文摘 | LAZAREV A FANG N LUO Q | 2003 | Rev Sci Instrum2003，74，8： | 1 |
| 11 | H-monotone operator and resolvent operator technique for variational inclusions显示文摘 | Huang N J Fang Y P | 2003 | Appl Math Comput2003，145，： | 1 |
| 12 | Relations between plasmaox-LDL and carotid plaque among Chinese Han ethnic group显示文摘 | Fang R Zhang N Wang C | 2011 | Neurol Res2011，33，5： | 1 |
| 13 | Impact of proteinuria and glo- merular filtration rate on risk of thromboembolism in atrial fibrilla- tion: the anticoagulation and risk factors in atrial fibrillation ( ATRIA ) study 显示文摘 | Go AS Fang MC Udaltsova N | 2009 | Circulation2009，119，10： | 1 |
| 14 | FeasibiLity and solvability of vector variational inequalities with moving cones in Ba- naeh spaces 显示文摘 | Fang Y P Huang N J | 2009 | Nonlinear Anal2009，70，5： | 1 |
| 15 | Epidemiologic features and environmental risk factors of severe fever with thrombocytopenia syndrome,Xinyang, China显示文摘 | Liu K Cui N Fang LQ | 2014 | PLoS Negl Trop Dis2014，8，5： | 1 |
| 16 | Cube resin insecticide:Identification and biolo- gical activity of 29 rotenoid constituents显示文摘 | FANG N CASIDA J E | 1999 | Journal of Agricultural and Food Chemistry1999，47，5： | 1 |
| 17 | Femtosecond laser fabrica-ted fiber bragg grating in microfiber for refractive index sensing显示文摘 | Fang X Liao C R Wang D N | 2010 | Optics Letters2010，35，7： | 1 |
| 18 | Projection micro-stereolithography using digital Micro-mirror dynamic mask 显示文摘 | Sun C Fang N D M Wu | 2005 | Sensors and Actuators A2005，121，： | 1 |
| 19 | Plasmonic nanolithography 显示文摘 | Srituravanich W Fang N Sun C | 2004 | Nano letters(S1530-6984)2004，4，6： | 1 |
| 20 | PET and SPECT imaging for stereotactic localization 显示文摘 | Fang B Mullani N | 1995 | Stereotact Funct Neurosurg1995，64，1： | 1 |
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