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39篇 您的检索式:作者名="ELSAYED A K"
    题名 作者 年代 出处 被引量
1视黄酸及Stra8基因联合作用对雄性鸡胚胎干细胞分化的影响显示文摘旨在研究视黄酸(All-trans retinoic acid,RA)及Stra8(Stimulated by retinoic acid gene 8)基因联合作用对雄性鸡胚胎干细胞(Chicken embryonic stem cells,cESCs)分化的影响。选用PCR方法鉴定雄性cESCs为研究对象,转染Stra 8基因,对Stra8基因阳性cESCs使用RA进行12d连续诱导,同时设立Stra8基因转染组、RA诱导组及空白组对照,各组均进行3次重复试验。细胞形态学、qRT-PCR检测RA及Sira8基因对cESCs分化的影响。结果表明。转染Stra8基因进行RA诱导,在诱导12d后,出现精原干细胞样的圆形小克隆,其他组cESCs出现类胚体或解体;qRT-PCR鉴定发现各组中干细胞标记基因表达量随着培养时间的延长逐渐下降,同时检测到生殖细胞相关基因表达,转染Stra8基因并使用RA诱导组中表达量均高于对照组(P<0.01),6d表达量达到最高,然后逐渐下降。Stra8基因和RA的联合作用能够促进cESCs向着生殖细胞方向的分化,不仅改变了原有细胞形态,同时出现了生殖相关基因的表达。通过该方法可以在体外建立一个。ESCs诱导分化模型,将为生殖细胞的发生、增殖、分化及调控机理的研究和人类不孕不育症的治疗奠定良好的基础。张振韬 王丹 施青青 ELSAYED A K 张亚妮 韦光辉 朱睿 左其生 李东 刘堃 李碧春 2014畜牧兽医学报2014,45,1:3
2Prognosticsignificance of CD20 expression and Epstein-Barr virus(EBV) association in classical Hodgkin lymphoma in Ja-pan :a clinicopathologic study 显示文摘Elsayed A A Asano N Ohshima K 2014Pathol Int2014,64,7:1
3Channel-aware earliest dead- line due fair scheduling for wireless multimedia networks 显示文摘ELSAYED K M F KHATI'AB A K F 2006Wire- less Personal Communications2006,38,1:1
4Two steroidal alkaloids from Veratrum viride显示文摘ELSAYED K A MCCHESNEY J D HALIM A F 1995Phytochemistry1995,38,6:1
5Design and construction of Q-switched Nd:YAG laser system for LIBS measurements显示文摘ELSAYED K IMAM H HARFOOSH A 2012Optics and Laser Technology2012,1,44:1
6Novel Multiplex PCR Assay for Characterization and Concomitant Subtyping of Staphylococcal Cassette Chromosome mec Types I to V in Methicillin-Resistant Staphylococcus aureus显示文摘Zhang K Y McClure J A Elsayed S 2005J Clin Microbiol2005,43,10:1
7Novel multiplex PCR assay for characterization and concomitant subtyping of staph- ylococcal cassette chromosome mec types I to V in Methieillin- resistant Staphylococcus aureus 显示文摘Zhang K McClure J A Elsayed S 2004J Clin Mierohiol2004,42,7:1
8Nutritional as- sessment of patients on hemodialysis in a large dial- ysis center 显示文摘A1 Saran K Elsayed S Molhem A 2011J Kidney Dis Transplant2011,,:1
9Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus 显示文摘Zhang K McClure J A Elsayed S 2005J Clin Microbiol2005,43,10:1
10Microstructures and mechanical responses of powder metallurgy non-combustive magnesium extruded alloy by rapid solidification process in mass production 显示文摘KONDOH K ELSAYED A IMAI H UMEDA J JONES T 2010Material Design2010,31,:1
11Novel multiplex PCR as- say for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methieillin-resistant Staphylococcus aureus显示文摘Zhang K Mcclure J A Elsayed S 2005J Clin Microbiol2005,43,10:1
12Novel ,taphylococcal cassette chromosome mec type, tentatively designated type Vm, har- boring class A mec and type 4 ccr gene complexes in a Canadian epidemic strain of methieillin resistant Staphylococcus aureus 显示文摘Zhang K McClure J A Elsayed S 2009Antimicrob Agents Chemother2009,53,2:1
13Estimation of thin-oxide reliability using proportional hazards models 显示文摘ELSAYED E A CHAN C K 1990IEEE Transactions on Reliability1990,39,3:1
14Extreme distribution for peak pressure and load coefficents 显示文摘Tieleman H W Elsayed M A K Ge Zhongfu 2008Journal of Wind Engineering and Industrial Aerodynamics2008,96,:1
15Crucial genes and pathways in chicken germ stem cell differ- entiation显示文摘ZHANG Z ELSAYED A K SHI Q 2015J Biol Chem2015,290,13:1
16Effect of mold and pouring temperature on the hot tearing of AZ91 magnesium alloy显示文摘Bichler L Lee K Elsayed A 2008Int J Metal Casting2008,1,2:1
17Universal scaling of plas-mon coupling in metal nanostructures: extensionfrom particle pairs to nanoshells 显示文摘Jain P K Elsayed M A 2007Nano Letters2007,7,9:1
18Influence of mold and pouring temperature on hot tearing susceptibility of AZ91D magnesium alloy 显示文摘BICHLER L ELSAYED A LEE K RAVINDRAN C 2008Int J Metalcasting2008,2,:1
19Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methieillin-resistant Staphylococcus aureus显示文摘ZHANG K MCCLURE J A ELSAYED S 2005J Clin Microbiol2005,43,10:1
20Design and construction of Q-switched Nd:YAG laser system for LIBS measurements 显示文摘Elsayed K Iman H Harfoosh A 2012Optics and Laser Technology2012,44,1:1
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