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| 1 | Climate and litter C/N ratio constrain soil organic carbon accumulation显示文摘Soil organic carbon(SOC) plays critical roles in stabilizing atmospheric CO2 concentration, but the mechanistic controls on the amount and distribution of SOC on global scales are not well understood. In turn, this has hampered the ability to model global C budgets and to find measures to mitigate climate change. Here, based on the data from a large field survey campaign with 2600 plots across China’s forest ecosystems and a global collection of published data from forested land, we find that a low litter carbon-to-nitrogen ratio(C/N) and high wetness index(P/PET, precipitation-to-potentialevapotranspiration ratio) are the two factors that promote SOC accumulation, with only minor contributions of litter quantity and soil texture. The field survey data demonstrated that high plant diversity decreased litter C/N and thus indirectly promoted SOC accumulation by increasing the litter quality. We conclude that any changes in plant-community composition, plant-species richness and environmental factors that can reduce the litter C/N ratio, or climatic changes that increase wetness index, may promote SOC accumulation. The study provides a guideline for modeling the carbon cycle of various ecosystem scales and formulates the principle for land-based actions for mitigating the rising atmospheric CO2 concentration. | Guoyi Zhou Shan Xu Philippe Ciais Stefano Manzoni Jingyun Fang Guirui Yu Xuli Tang Ping Zhou Wantong Wang Junhua Yan Gengxu Wang Keping Ma Shenggong Li Sheng Du Shijie Han Youxin Ma Deqiang Zhang Juxiu Liu Shizhong Liu Guowei Chu Qianmei Zhang Yuelin Li Wenjuan Huang Hai Ren Xiankai Lu Xiuzhi Chen | 2019 | National Science Review2019，6，4： | 21 |
| 2 | In vitro culture and differentiation of rat embryonic midbrain-derived neural stem cells显示文摘BACKGROUND:Midbrain-derived neural stem cells (mNSCs) can differentiate into functional mature dopaminergic neurons. The mNSCs are considered the ideal choice for cell therapy of Parkinson’s disease. OBJECTIVE: To isolate rat embryonic mNSCs and to observe the differentiation characteristics of mNSCs induced by cell growth-promoting factors. DESIGN,TIME AND SETTING: An in vitro cell culture study based on the molecular biology of nerve cells was carried out at the Institute of Clinical Medicine,China-Japan Friendship Hospital (China) from March to November 2007. MATERIALS: Sprague Dawley rats at embryonic day 14 were used in this study. Nestin antibody,β-Ⅲ tubulin antibody,glial fibrillary acidic protein (GFAP) antibody and cyclic nucleotide 3’-phosphohydrolase (CNPase) antibody were provided by Abcam; DMEM/F12 medium and N2 supplement were provided by In-vitrogen; epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF2) were provided by R&D Sys-tems. METHODS: The ventral mesencephalon was dissected from embryonic day 14 rat embryos. By trypsin di-gestion and mechanical separation,the brain tissue was triturated into a fine single-cell suspension. The cells were cultured in 5 mL serum-free medium containing DMEM/F12,1% N2 supplement,20 ng/mL EGF and FGF2. The mNSCs at the third generation were coated with 10 μg/mL polylysine and induced to differenti-ate in the DMEM/F12 supplemented with 1% fetal bovine serum and 1% N2. MAIN OUTCOME MEASURES: The neural spheres of the third passage were identified by nestin im-munofluorescence; at the same time,the cells were induced to differentiate,and the types of differentiated cell were identified by immunofluorescence for βⅢ tubulin,GFAP and CNPase. RESULTS: Seven days after primary culture,a great many neurospheres could be obtained by successive pasage. Immunofluorescence assays showed that the neurospheres were nestin positive,and after differentia-tion,the cells expressed GFAP,CNPase and β-Ⅲ-tubulin. CONCLUSION: Embryonic day 14 rat mNSCs can differentiate into neuron-like cells and glial cells fol-lowing induction by EGF,FGF2 and N2 additive. | Xingli Deng Ruen Liu Zhongtang Feng Jing Guo Wu Wang Deqiang Lei Hongyan Li Zhihua Chen | 2008 | Neural Regeneration Research2008，3，11： | 19 |
| 3 | Effect of N and P addition on soil organic C potential mineralization in forest soils in South China显示文摘Atmospheric nitrogen deposition is at a high level in some forests of South China. The effects of addition of exogenous N and P on soil organic carbon mineralization were studied to address: (1) if the atmospheric N deposition promotes soil C storage through decreasing mineralization; (2) if the soil available P is a limitation to organic carbon mineralization. Soils (0-10 cm) was sampled from monsoon evergreen broad-leaved forest (MEBF), coniferous and broad-leaved mixed forest (CBMF), and Pinus massoniana forest (PMF) in Dinghushan Biosphere Reserve (located in Guangdong Province, China). The soils were incubated at 25°C for 45 weeks, with addition of N (NH4NO3 solution) or P (KH2PO4 solution). CO2-C emission and the inorganic N (NH4+-N and NO3?-N) of the soils were determined during the incubation. The results showed that CO2-C emission decreased with the N addition. The addition of P led to a short-term sharp increase in CO2 emission after P application, and the responses of CO2-C evolution to P addition in the later period of incubation related to forest types. Strong P inhibition to CO2 emission occurred in both PMF and CBMF soils in the later incubation. The two-pool kinetic model was fitted well to the data for C turnover in this experiment. The model analysis demonstrated that the addition of N and P changed the distribution of soil organic C between the labile and recalcitrant pool, as well as their mineralization rates. In our experiment, soil pH can not completely explain the negative effect of N addition on CO2-C emission. The changes of soil inorganic N during incubation seemed to support the hypothesis that the polymerization of added nitrogen with soil organic compound by abiotic reactions during incubation made the added nitrogen retard the soil organic carbon mineralization. We conclude that atmospheric N deposition contributes to soil C accretion in the three subtropical forest ecosystems, however, the shortage of soil available P in CBMF and PMF may also retard soil organic C mineralization. | OUYANG, Xuejun ZHOU, Guoyi HUANG, Zhongliang ZHOU, Cunyu LI, Jiong SHI, Junhui ZHANG, Deqiang | 2008 | Journal of Environmental Sciences2008，20，9： | 17 |
| 4 | Primary structure of helix C to helix G of a new retinal protein in H.sp.xz515显示文摘The gene fragment encoding the retinal protein from helix C to helix G in a new strain of halobacteria, H.sp.xz515 has been amplified by PCR method. The nucleotide sequence of this fragment has been determined. The deduced amino acid sequence has been compared with halobium br and other two br-like proteins, ar-1 and ar-2. Results show that those amino acid residues in br, essential for proton pumping and binding to retinal, are conserved. The residue M145 in br may be important for isomerization reaction of retinal. | Hui Wang Shuxuan Zhan Qingan Sun Deqiang Xu Wei Zhao Weida Huang Qingguo Li | 2000 | Chinese Science Bulletin2000，45，12： | 5 |
| 5 | Free radical scavenging activity of Eagle tea and their flavonoids显示文摘In this study,an online HPLC-DAD-MS coupled with 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)diammonium salt(A BTS)assay was employed for evaluating free radical scavenging activity of Eagle tea and their active components.Twenty-three chromatographic peaks were detected,and nineteen components had free radical scavenging activity.Among them,eight compounds were identified as flavonoids(hyperin,isoquercitrin,quercitrin,quercetin,kaempferol),catechins,chlorogenic acid and epicatechin based on MS data and standard chromatographic characters. | Qiong Meng Zhengming Qian Xiaoxiang Li Deqiang Li Weihua Huang Jing Zhao Shaoping Li | 2012 | Acta Pharmaceutica Sinica B2012，2，3： | 4 |
| 6 | Effects of Rubber Shock Absorber on the Flywheel Micro Vibration in the Satellite Imaging System显示文摘当一颗卫星在轨道时,它的飞轮将产生微颤动并且影响照相机的成像质量。以便减少这效果,一个橡胶减震器被使用,并且一个数字模型和试验性的安装被开发在学习在微颤动上调查它的效果。一个综合模型为系统被开发,并且跟踪方法的一条光线在建模被使用。图象飞机的点坐标和排水量被获得,并且系统的 modulate 转移函数(MTF ) 是计算的。包括一个橡胶减震器的一颗卫星被设计,并且实验被执行。模拟和实验结果证明 MTF 增加几乎 10 % ,建议橡胶减震器是有用的减少飞轮颤动。 | Changcheng DENG Deqiang MU Xuezhi JIA Zongxuan LI | 2016 | Photonic Sensors2016，6，4： | 4 |
| 7 | Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak显示文摘Background:Spermatogenesis is a cellular differentiation process that includes three major events:mitosis of spermatogonia,meiosis of spermatocytes and spermiogenesis.Steady-state spermatogenesis relies on functions of spermatogonial stem cells(SSCs).Establishing and maintaining a foundational SSC pool is essential for continued spermatogenesis in mammals.Currently,our knowledge about SSC and spermatogenesis is severely limited in domestic animals.Results:In the present study,we examined transcriptomes of testes from domestic yaks at four different stages(3,5,8 and 24 months of age)and attempted to identify genes that are associated with key developmental events of spermatogenesis.Histological analyses showed that the most advanced germ cells within seminiferous tubules of testes from 3,5,8 and 24 months old yaks were gonocytes,spermatogonia,spermatocytes and elongated spermatids,respectively.RNA-sequencing(RNA-seq)analyses revealed that 11904,4381 and 2459 genes were differentially expressed during the gonocyte to spermatogonia transition,the mitosis to meiosis transition and the meiosis to post-meiosis transition.Further analyses identified a list of candidate genes than may regulate these important cellular processes.CXCR4,a previously identified SSC niche factor in mouse,was one of the up-regulated genes in the 5 months old yak testis.Results of immunohistochemical staining confirmed that CXCR4 was exclusively expressed in gonocytes and a subpopulation of spermatogonia in the yak testis.Conclusions:Together,these findings demonstrated histological changes of postnatal testis development in the domestic yak.During development of spermatogonial lineage,meiotic and haploid germ cells are supported by dynamic transcriptional regulation of gene expression.Our transcriptomic analyses provided a list of candidate genes that potentially play crucial roles in directing the establishment of SSC and spermatogenesis in yak. | Guowen Wang Yongchang Li Qilin Yang Shangrong Xu Shike Ma Rongge Yan Ruina Zhang Gongxue Jia Deqiang Ai Qi’en Yang | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 3 |
| 8 | Comparison of Nonlinear Local Lyapunov Vectors with Bred Vectors, Random Perturbations and Ensemble Transform Kalman Filter Strategies in a Barotropic Model显示文摘The breeding method has been widely used to generate ensemble perturbations in ensemble forecasting due to its simple concept and low computational cost. This method produces the fastest growing perturbation modes to catch the growing components in analysis errors. However, the bred vectors(BVs) are evolved on the same dynamical flow, which may increase the dependence of perturbations. In contrast, the nonlinear local Lyapunov vector(NLLV) scheme generates flow-dependent perturbations as in the breeding method, but regularly conducts the Gram–Schmidt reorthonormalization processes on the perturbations. The resulting NLLVs span the fast-growing perturbation subspace efficiently, and thus may grasp more components in analysis errors than the BVs.In this paper, the NLLVs are employed to generate initial ensemble perturbations in a barotropic quasi-geostrophic model.The performances of the ensemble forecasts of the NLLV method are systematically compared to those of the random perturbation(RP) technique, and the BV method, as well as its improved version—the ensemble transform Kalman filter(ETKF)method. The results demonstrate that the RP technique has the worst performance in ensemble forecasts, which indicates the importance of a flow-dependent initialization scheme. The ensemble perturbation subspaces of the NLLV and ETKF methods are preliminarily shown to catch similar components of analysis errors, which exceed that of the BVs. However, the NLLV scheme demonstrates slightly higher ensemble forecast skill than the ETKF scheme. In addition, the NLLV scheme involves a significantly simpler algorithm and less computation time than the ETKF method, and both demonstrate better ensemble forecast skill than the BV scheme. | Jie FENG Ruiqiang DING Jianping LI Deqiang LIU | 2016 | Advances in Atmospheric Sciences2016，33，9： | 3 |
| 9 | Study of Cracking Mechanism and Wear Resistance in Laser Cladding Coating of Ni-based Alloy显示文摘Nickel-based alloy coatings were widely used for the remanufacturing of dies and moulds by laser cladding,but the crack sensitivity would be increase due to the higher strength and hardness,which reduced the wear resistance of Ni-based alloys.In this paper,Ni-based coatings with the addition of a plastic phase(an austenitic stainless net)were prepared using laser cladding technology,and the CeO_(2)was added in cladding layers.The cracking mechanism,microhardness,microstructure,phase composition,and wear properties were investigated.The relationship between thermal stress and the elastic and plastic fracture had been developed from the standpoint of fracture mechan-ics and thermal elastic fracture mechanics.The fracture criterion of the nickel-based coating was obtained,and the study has shown that the crack sensitivity could be reduced by decreasing the thermal expansion coefficientΔα.Thus,a new method was proposed,which the stainless steel nets were prefabricated on the substrate.It was found that the number of cracks reduced significantly with the addition of stainless steel net.When the stainless steel net with 14 mesh was added in Ni-based coatings,the average microhardness of nickel composite coating was 565 HV_(0.2),which was 2.6 times higher than that of the 45 steel substrate.Although the rare earth oxide 4 wt.%CeO_(2)and stainless steel net were added in the Ni-based coating reducing the microhardness(the average microhardness is 425 HV_(0.2)),the wear resistance of it improved substantially.The wear volume of Ni-based composite coating was 0.56×10^(−5) mm^(3)·N^(−1)·m^(−1),which was 85.1%lower than that of 45 steel.The experiment results have shown that the Nickel-based composite coating is equipped with low crack sensitivity and high abrasive resistance with austenitic stainless net and the rare earth oxide 4 wt.%CeO2.This research offers an efficient solution to produce components with low crack susceptibility and high wear-resistance coatings fabricated by laser cladding. | Zhenglei Yu Lunxiang Li Deqiang Zhang Guangfeng Shi Guang Yang Zezhou Xu Zhihui Zhang | 2021 | Chinese Journal of Mechanical Engineering2021，34，5： | 2 |
| 10 | Crystal structure of a bacterial homolog to human lysosomal transporter,spinster显示文摘Lysosomes break down various biomolecules and spinster is one of the major efflux carriers removing degradation products from lysosomal lumen to keep it in healthy size and proper function.Although it is well established that a dysfunctional spinster will cause enlarged lysosomes and in turn lead to developmental defects and abnormal behavior in animals,little was known about the transportation mechanism and substrate specificity of spinster.Here,we report a crystal structure of spinster homolog from Hyphomonas neptunium,HnSPNS,in its inward-facing conformation with and without substrate bound.HnSPNS is crystallized in a monomer and a substrate-binding cavity was formed in the center of its transmembrane helices.A blob of electron density corresponding to its substrate was found in the cavity near a conserved residue,R42,which is locked in position by the interactions with conserved residues E129 and R122.Our results suggest that human spinster serves as a transporter translocating negativelycharged lipophilic small molecules and E129 might serve as a switch to control the conformational change via its protonation-deprotonation cycle. | Fu Zhoua Deqiang Yao Bing Rao Li Zhang Wang Nie Yan Zou Jie Zhao Yu Cao | 2019 | Science Bulletin2019，64，18： | 2 |
| 11 | Effects of flow shear stress and mass transport on the construction of a large- scale tissue-engineered bone in a perfusion bioreactor 显示文摘 | Li Deqiang Tang Tingting Lu Jianxi | 2009 | Tissue Engineering: Part A2009，15，10： | 1 |
| 12 | Brominelpara-Toluenesulfonic Acid-Catalyzed Synthesis of 3,3-Bis(indole-3-yl)indoline-2-(1H)-ones by Condensing Indoles with Isatins显示文摘在仅仅在房间温度的 Br 2 的 3 mol% 的催化作用下面,吲哚与靛红很快反应了形成生物学上重要的 3,3 二度(indole-3-yl ) 有高效率和宽底层范围的 indoline-2-(1H ) 。而且,我们证明 p-toluenesulfonic 酸(TsOH ) 能用作一个代理人催化这转变。 | Wenzhong Huang Lingli Nang Xiangguang Li Lin Yuan Yinhai Ma Deqiang Liang | 2015 | Chinese Journal of Chemistry2015，33，10： | 1 |
| 13 | A meta-analysis of DNA repair gene XPC polymorphisms and cancer risk显示文摘 | Deqiang Zhang Chengwen Chen Xuping Fu Shaohua Gu Yumin Mao Yi Xie Yan Huang Yao Li | 2008 | Journal of Human Genetics2008，，1： | 1 |
| 14 | Epigenomic profiling of young and aged HSCs reveals concerted changes during aging that reinforce self-renewal显示文摘 | Deqiang Sun Min Luo Mira Jeong Benjamin Rodriguez Zheng Xia Rebecca Hannah Hui Wang Thuc Le Kym F. Faull Rui Chen Hongcang Gu Christoph Bock Alex Meissner Berthold G?ttgens Gretchen J. Darlington Wei Li Margaret A. Goodell | 2014 | Cell Stem Cell2014，，： | 1 |
| 15 | Folate-conjugated pH-responsive nanocarrier designed for active tumor targeting and controlled release of doxorubicin显示文摘 | Lulu WEI Beibei LU Lin CUI Xueying PENG Jianning wu Deqiang LI Zhiyong LIU Xuhong GUO | 2017 | Frontiers of Materials Science2017，11，4： | 1 |
| 16 | Virtual power plant-based distributed control strategy for multiple distributed generators显示文摘 | Xin Huanhai Gan Deqiang Li Naihu | 2013 | Control Theory & Applications IET2013，7，1： | 1 |
| 17 | Pharmacokinetics and tissue distribution study of orientin in rat by liquid chromatog- raphy显示文摘 | Li Deqiang Wang Qiao Yuan Zhifang | 2008 | J Pharm Biomed Anal2008，47，2： | 1 |
| 18 | ZmWRKY33 , a WRKY maize transcription factor conferring enhanced salt stress tolerances in Arabidopsis显示文摘 | Hua Li Yong Gao Hu Xu Yi Dai Deqiang Deng Jianmin Chen | 2013 | Plant Growth Regulation2013，，3： | 1 |
| 19 | Null genotypes of GSTM1 and GSTT1 contribute to hepatocellular carcinoma risk: Evidence from an updated meta-analysis显示文摘 | Bin Wang Gang Huang Dan Wang Aijun Li Zhipeng Xu Ran Dong Deqiang Zhang Weiping Zhou | 2010 | Journal of Hepatology2010，，3： | 1 |
| 20 | Experimental and theoretical study of the charge transport property of 4,4′-N,N′-dicarbazole-biphenyl显示文摘The hole and electron mobilities of the amorphous films of the organic semiconductor 4,4′-N,N′-dicarbazole-biphenyl (CBP) at different electric fields were measured through the time of flight (TOF) method. Based on its crystalline structure, the hole and electron mobilities of CBP were calculated. A detailed comparison between experimental and theoretical results is necessary for further understanding its charge transport properties. In order to do this, charge mobilities at zero electric field, μ(0), were deduced from experimental data as a link between experimental and theoretical data. It was found that the electron transport of CBP is less affected by traps compared with its hole transport. This unusual phenomenon can be understood through the distributions of frontier molecular orbitals. We showed that designing materials with frontier molecular orbitals localized at the center of the molecule has the potency to reduce the influence of traps on charge transport and provide new insights into designing high mobility charge transport materials. | LI HaoYuan CHEN Liang QIAO Juan DUAN Lian ZHANG DeQiang DONG GuiFang WANG LiDuo QIU Yong | 2012 | Science China Chemistry2012，55，11： | 1 |
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