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您的检索式：作者名="Debabrata Laha"
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| 1 | ITPK1 is an InsP_(6)/ADP phosphotransferase that controls phosphate signaling in Arabidopsis.显示文摘In plants, phosphate (Pi) homeostasis is regulated by the interaction of PHR transcription factors with stand-alone SPX proteins, which act as sensors for inositol pyrophosphates. Here, we combined different methods to obtain a comprehensive picture of how inositol (pyro)phosphate metabolism is regulated by Pi and dependent on the inositol phosphate kinase ITPK1. We found that inositol pyrophosphates are more responsive to Pi than lower inositol phosphates, a response conserved across kingdoms. With CE-ESI-MS we could separate different InsP7 isomers in Arabidopsis and rice, and identify 4/6-InsP7 and a PP-InsP4 isomer hitherto not reported in plants. We found that the inositol pyrophosphates 1/3-InsP7, 5-InsP7 and InsP8 increase severalfold in shoots after Pi resupply and that tissue-specific accumulation of inositol pyrophosphates relies on ITPK1 activities and MRP5-dependent InsP6 compartmentalization. Notably, ITPK1 is critical for Pi-dependent 5-InsP7 and InsP8 synthesis in planta and its activity regulates Pi starvation responses in a PHR-dependent manner. Furthermore, we demonstrate that ITPK1-mediated conversion of InsP6 to 5-InsP7 requires high ATP concentrations and that Arabidopsis ITPK1 has an ADP phosphotransferase activity to dephosphorylate specifically 5-InsP7 under low ATP. Collectively, our study provides deeper insights into Pi-dependent changes in nutritional and energetic states with the synthesis of regulatory inositol pyrophosphates. | Esther Riemer Danye Qiu Debabrata Laha Robert K.Harmel Philipp Gaugler Verena Gaugler Michael Frei Mohammad-Reza Hajirezaei Nargis Parvin Laha Lukas Krusenbaum Robin Schneider Adolfo Saiardi Dorothea Fiedler Henning J.Jessen Gabriel Schaaf Ricardo F.H.Giehl | 2021 | Molecular Plant2021，14，11： | 0 |
| 2 | TOM9.2 Is a Calmodulin-Binding Protein Critical for TOM Complex Assembly but Not for Mitochondrial Protein Import in Arabidopsis thaliana显示文摘线粒体(TOM ) 的外部膜上的 translocon 便于原子编码蛋白质的进口。mitochondrial 蛋白质运输的主要机械似乎在优核质保存了;然而,在 TOM 部件的作文和结构的分叉在哺乳动物,酵母,和植物之间被观察了。TOM9,酵母 Tom22 的植物相当或相同的事物,由于在 cytosolic 受体域,和它的精确函数的截断是显著地更小的没被理解。这里,我们提供证明从 Arabidopsis thaliana 的 TOM9.2 涉及成熟 TOM 建筑群的形成的证据,由影响形成毛孔的子单元 TOM40 的集会最可能。在在成熟 TOM 建筑群的严重减小的 TOM9.2 结果的导致 Dexamethasone 的 RNAi 基因 silencing,和进建筑群的最新进口的 TOM40 的集会被损害。不过,变异的植物是 TOM 建筑群的损失的充分可行、不明显的下游的效果,即在 mitochondrial 进口能力上,被观察。而且,我们发现 TOM9.2 能在 vitro 绑 calmodulin (凸轮) ,那个凸轮损害在孤立的野类型的线粒体复杂的 TOM 的集会,建议 TOM9.2 的一个规章的角色和进表明网络的细胞的钙的 TOM 汇编的可能的集成。 | Nargis Parvin Chris Carrie Isabelle Pabst Antonia Laber Debabrata Laha Melanie V. Paul Peter Geigenberger Ralf Heermann Kirsten Jung Ute C. Vothknecht Fatima Chigri | 2017 | Molecular Plant2017，10，4： | 0 |
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