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| 1 | Cell–cell contact with proinflammatory macrophages enhances the immunotherapeutic effect of mesenchymal stem cells in two abortion models显示文摘Mesenchymal stem cells(MSCs),which are pluripotent cells with immunomodulatory properties,have been considered good candidates for the therapy of several immune disorders,such as inflammatory bowel diseases,concanavalin A-induced liver injury,and graft-versus-host disease.The embryo is a natural allograft to the maternal immune system.A successful pregnancy depends on the timely extinction of the inflammatory response induced by embryo implantation,followed by the switch to a tolerant immune microenvironment in both the uterus and the system.Excessive infiltration of immune cells and serious inflammatory responses are triggers for embryo rejection,which results in miscarriage.Here,we demonstrated that adoptive transfer of MSCs could prevent fetal loss in a lipopolysaccharide(LPS)-induced abortion model and immune response-mediated spontaneous abortion model.The immunosuppressive MSCs alleviated excessive inflammation by inhibiting CD4+T cell proliferation and promoting the decidual macrophage switch to M2 in a tumor necrosis factor-stimulated gene-6(TSG-6)-dependent manner.Cell-tocell contact with proinflammatory macrophages increased the TSG-6 production by the MSCs,thereby enhancing the suppressive regulation of T cells and macrophages.Moreover,proinflammatory macrophages in contact with the MSCs upregulated the expression of CD200 on the stem cells and facilitated the reprogramming of macrophages towards an anti-inflammatory skew through the interaction of CD200 with CD200R on proinflammatory macrophages.Therefore,the results demonstrate that a TSG-6-mediated paracrine effect,reinforced by cell-to-cell contact between MSCs and proinflammatory macrophages,is involved in the mechanism of MSC-mediated abortion relief through the induction of immune tolerance.Our study also indicates the potential application of MSCs in clinical recurrent miscarriages. | Yanhong Li Di Zhang Ling Xu Lin Dong Ji Zheng Yikong Lin Jiefang Huang Yanyun Zhang Yu Tao Xingxing Zang Dajin Li Meirong Du | 2019 | Cellular & Molecular Immunology2019，16，12： | 23 |
| 2 | Dehydroepiandrosterone Inhibited the Bone Resorption through the Upregulation of OPG/RANKL显示文摘dehydroepiandrosterone (DHEA ) 的血浆水平与老化一起逐渐地是减少。DHEA 的有益的效果作为反老化类固醇,反糖尿病 mellitus,反动脉粥样硬化,反痴呆,反肥胖和反骨质疏松症例如免疫系统上的 stimulatory 效果,在 vitro 并且在 vivo 在实验被表明了。为绝经后的骨质疏松症在治疗学调查 DHEA 的有效机制是重要的。孤立并且有教养的造骨细胞( Obs )和骨破折( Ocs ),我们在 osteoblastic 生存能力上分析了 DHEA 的效果, osteoprotegerin ( OPG )的表示上的 DHEA 的规定 NF-B ligand ( RANKL )的 /receptor 使活跃之物在 Obs 的 mRNA ,然后当面在 Ocs 的骨头再吞上观察了 DHEA 的行动或 Obs 的缺席。结果证明 DHEA 在 10-8-10-6 M 的集中范围以内改进了 Obs 的生存能力,特别在 10-7 M 的集中。DHEA 能显然在 Obs 增加 OPG/RANKL mRNA 的比率。面对 Obs, DHEA 能减少 specula 的吸收空隙的数字和区域。因此,我们仅仅面对 Obs 得出结论, DHEA 能禁止 Ocs 的骨头再吞,它可以被 Obs 的 OPG/RANKL 调停。 | Yudong Wang Ling Wang Dajin Li Wenjun Wang | 2006 | Cellular & Molecular Immunology2006，3，1： | 11 |
| 3 | SCM-198 protects endometrial stromal cells from oxidative damage through Bax/Bcl-2 and ERK signaling pathways显示文摘In creasi ng amounts of evidence dem on strated that accumulative reactive oxyge n species (ROS) and apoptosis of human endometrial stromal cells (ESCs) are closely associated with endometrial dysfunction induced by oxidative stress, which plays an important role in the pathological process of multiple gyn ecological and reproduction-related diseases. SCM-198, an alkaloid active component of Leonurus japonicas Houtt, has been reported to have anti-oxidative activity. However, the specific mechanisms of SCM-198 in the prevention of endometrial damage remain unknown. In the present study, we assessed the effect of SCM-198 on hydrogen peroxide (H2O2Hnduced oxidative injury in ESCs. ESCs were pretreated with SCM-198 for 4 h and then challenged with H2O2.Morphology ch a nges, apoptosis rate, and intracellular ROS producti on were measured to assess the level of oxidative injury. Flow cytometry and western blot analysis were performed to detect the expression levels of Bax, Bcl-2, active-caspase-3, and mitogen-activated protein kinases pathways. Classic inflammation cytokines were measured by real-time polymerase chain reactions. Our results showed that SCM-198 attenuated apoptosis and ROS generation of ESCs induced by H2O2. H2O2 induced the apparent apoptotic characteristics, including fragmentation of DNA, upregulation of Bax/Bcl2, activation of caspase-3, and secretion of inflammation cytokines, which were all ameliorated by SCM-198. Furthermore, H2O2-induced apoptosis-related ERK1/2 pathway activation was restrained by SCM-198 pretreatme nt. These fin dings suggested that SCM-198 could protect ESCs from oxidative injury, mainly by inhibiting oxidative stress and reducing apoptosis. | Yunyun Li Yikong Lin Xixi Huang Chunfang Xu Xinhua Liu Li Wang Min Yu Dajin Li Yizhun Zhu Meirong Du | 2019 | Acta Biochimica et Biophysica Sinica2019，51，6： | 10 |
| 4 | Activation of Rev-erbα attenuates lipopolysaccharide-induced inflammatory reactions in human endometrial stroma cells via suppressing TLR4-regulated NF-κB activation显示文摘Perturbation of the circadian rhythm damages the biological characteristics of cells and leads to their dysfunction. Rev-erbα, an important gene in the transcription-translation loop of circadian rhythm, is involved in regulating the balance between pro-inflammation and anti-inflammation. The disruption of this balance in human endometrial stroma cells (hESCs) destroys their biological behavior function in maintaining the menstrual cycle and embryonic implantation. Whether pharmacological modulation of Rev-erbα affects the inflammation of hESCs remains unclear. In this study, we treated hESCs with lipopolysaccharide (LPS) and found that LPS treatment increased the mRNA levels of pro-inflammatory cytokines, such as interleukin (IL)-1β, IL-6, IL-8, IL-18, and TNFα, and the secretion of IL-6. SR9009, a Rev-erbα agonist, significantly alleviated the LPS-induced production of pro-inflammatory cytokines in hESCs. Meanwhile, knockdown of Rev-erbα increased the expressions of IL-1β, IL-6, and IL-8, accompanied by an increased mRNA level of the core clock gene Bmal1. Western blot analysis showed that SR9009 inhibited the expression of toll-like receptor 4 (TLR4) and the activation of NF-κB induced by LPS. All these findings suggested that pharmacological activation of Rev-erbα attenuated the LPS-induced inflammatory response of hESCs by suppressing TLR4-regulated NF-κB activation. This study may provide a strategy for preventing inflammation-related endometrial dysfunction and infertility or recurrent implantation failure. | Weijie Zhao Liyuan Cui Xixi Huang Songcun Wang Dajin Li Liping Li Yan Sun Meirong Du | 2019 | Acta Biochimica et Biophysica Sinica2019，51，9： | 8 |
| 5 | Regulatory mechanisms of endometrial decidualization and pregnancy-related diseases显示文摘Endometrial decidualization is one of the earlest changes by which the uterus adapts to pregnancy.During this period,the endometrium undergoes complex changes in its biochemistry,physiology,and function at various levels,providing a suitable microenvironment for embryo implantation and development.Favorable decidualization lays an essential foundation for subsequent gestation,without which pregnancy failure or pregnancy complications may occur.The interaction between pregnancy-related hormones and cytokines produced by embryonic and uterine cells is known to be essential for decidualization,in which some transcription factors also play pivotal roles.Increasing evidence has revealed the importance of metabolism in regulating decidualization.Here,we summarize and discuss these crucial elements in decidualization and the relationship between decidualization and pregnancy complications.A better comprehension of these issues should help to improve the prediction of pregnancy outcomes and the use of appropriate intervention. | Yifei Sang Yanhong Li Ling Xu Dajin Li Meirong Du | 2020 | Acta Biochimica et Biophysica Sinica2020，52，2： | 4 |
| 6 | Abnormal regulation of chemokine TECK and its receptor CCR9 in the endometriotic milieu is involved in pathogenesis of endometriosis by way of enhancing invasiveness of endometrial stromal cells显示文摘The chemokine thymus-expressed chemokine(TECK),which regulates T-cell development and tissue-specific homing,has been identified as a potential contributor to the pathogenesis and progression of endometriosis.Dioxin(2,3,7,8-tetrachlorodibenzo-p-dioxin,TCDD),an air pollutant,and estrogen also appear to be involved in endometriosis.Both endometrial stromal cells(ESCs)and the combination of 17b-estradiol and TCDD increase the secretion of TECK in the endometriosis-associated cells and promote the invasiveness of ESCs by increasing expression of matrix metalloproteinase(MMP)-2 and MMP-9.Anti-TECK neutralizing antibodies can effectively inhibit the invasiveness of ESCs and the expression of MMP-2 and MMP-9 in the cells.Interestingly,the expression of chemokine C receptor 9(CCR9)and its ligand TECK increases significantly in the endometriotic milieu of patients with endometriosis.Therefore,the over-expressed TECK interacts with CCR9 on the ESCs in the endometriotic milieu,which may contribute to the onset and progression of endometriosis. | Yun Wang Jing Yu Xuezhen Luo Xiaoqiu Wang Mingqing Li Ling Wang Dajin Li | 2010 | Cellular & Molecular Immunology2010，7，1： | 3 |
| 7 | A novel thermometric biosensor for fast surveillance of β-lactamase activity in milk显示文摘 | Shuang Zhou Yunfeng Zhao Michael Mecklenburg Dajin Yang Bin Xie | 2013 | Biosensors and Bioelectronics2013，，： | 2 |
| 8 | Effect of the cross-correlation between the real and imaginary parts of the quantum noise in the transient properties of single-mode laser显示文摘 | Zhang Li Cao Li Wu Dajin | 2003 | Phys Lett A2003，309，： | 1 |
| 9 | Study of cogging torque in surface-mounted permanent magnet motors with energy method显示文摘 | Wang Xiuhe Yang Yubo Fu Dajin | 2003 | Journal of Magnetism Material2003，267，1： | 1 |
| 10 | Dynamic for system driven by correlated colored noise显示文摘 | Wei Xueqin Cao Li Wu Dajin | 1995 | Phys Lett A1995，207，： | 1 |
| 11 | Theory of nonlinear external noise显示文摘 | Cao Li Wu Dajin Wan Huixian | 1988 | Phys Lett A1988，133，： | 1 |
| 12 | Study of cogging torque in surface-mounted permanent magnet motors with energy method显示文摘 | Wang Xiuhe Yang Yubo Fu Dajin | 2003 | Journal of Magnetism and Magnetic Material2003，267，11： | 1 |
| 13 | TLR4 is required for the obesity-induced pancreatic beta cell dysfunction显示文摘肥胖是为类型 2 糖尿病的一个重要导致因素。然而,位于 high-fat-(HF ) 下面的机制在胰腺的贝它房间机能障碍的导致食谱的肥胖仍然是不清楚的。像使用费的 receptor-4 (TLR4 ) 是天生的免疫的一个关键调停人。在导致肥胖的胰腺的贝它房间机能障碍调查 TLR4 的效果,我们使用了男糖尿病患者(db/db ) ,肥胖(ob/ob ) 老鼠, TLR4 野的类型(WT ) ,和用正常饮食或 HF 被喂的 TLR4 大美人老鼠节食 24 个星期。在胰腺的小岛的 TLR4 和 TLR4 mRNA 水平的 Immunostaining 被估计。从生物特征,葡萄糖忍耐测试,胰岛素忍耐测试,和胰岛素版本测试的结果证明胰腺的小岛的功能在喂 HF 的 TLR4 WT 老鼠被损害,但是在喂 HF 的 TLR4 被保护缺乏(TLR4/) 老鼠。由电子显微镜察觉,我们观察了能分泌的泡在喂 HF 的 TLR4 WT 老鼠增加了的那贝它房间胰岛素。在喂 HF 的 TLR4/ 老鼠的贝它房间的超微结构在正常食物类似于那喂食谱的 TLR4 WT 老鼠。然后,由使用主要胰腺的小岛的刺激葡萄糖的胰岛素分泌物试金证明在喂 HF 的 TLR4/ 鼠标的胰腺的小岛的分泌物函数在喂 HF 的 TLR4 WT 鼠标比那好。而且,在喂 HF 的 TLR4/ 老鼠, IL-6 的 mRNA 层次, TNF- ,和 MCP-1,在胰腺的小岛的基因是比在喂 HF 的 TLR4 WT 老鼠的那些显著地低的。与在基因表示,喂 HF 的 TLR4 WT 老鼠然而并非喂 HF 的 TLR4/ 的变化一致老鼠在胰腺的岛展出了巨噬细胞侵略。一起拿,我们的数据显示了那 HF 导致食谱的肥胖能刺激在胰腺的贝它房间的表面上定位的 TLR4 的起来规定,并且随后导致巨噬细胞的招募进胰腺的小岛,它最后导致胰腺的贝它房间机能障碍。这个过程是涉及导致肥胖的胰腺的贝它房间机能障碍的可能的机制。 | Juan Li Lei Chen Ye Zhang Weiping J. Zhang Wenyan Xu Yi Qin Jin Xu Dajin Zou | 2013 | Acta Biochimica et Biophysica Sinica2013，45，12： | 1 |
| 14 | invisible linkages: a regional perspective of East Asian political economy 显示文摘 | Dajin Peng | 2002 | International studies2002，，46： | 1 |
| 15 | Study of Cogging Torque inSurface-Mounted Permanent Magnet Motors with Energy Method显示文摘 | Wang Xiuhe Yang Yubo Fu Dajin | 2003 | Journal of Magnetism and Magnetic Material2003，267，11： | 1 |
| 16 | Simultaneous determination of nonnutritive Sweeteners in Foods by HPLC/ESI-MS 显示文摘 | YANG Dajin CHEN Bo | 2009 | J Agricul Food Chem2009，57，8： | 1 |
| 17 | Cross-correlation of multiplicative and additive noises in a single-mode laser white-gain-noise model and correlated noises induced transitions显示文摘 | Cao Li Wu Dajin | 1999 | Phys Lett A1999，260，： | 1 |
| 18 | General laser intensity Langevin equation in a single-mode laser显示文摘 | Ke Shengzhi Cao Li Wu Dajin | 2001 | Chin Phys Lett2001，18，3： | 1 |
| 19 | The research of single-mode laser driven by multiplicative colored noise with cross-correlation between the real and imaginary parts of the quantum noise显示文摘 | Zhang Li Cao Li Wu Dajin | 2004 | Physica A2004，332，： | 1 |
| 20 | A power-balancing scheme for clustered wireless sensor networks 显示文摘 | Wang Dajin | 2007 | FGCN2007，，： | 1 |
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