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| 1 | 雪藻高密度连续培养中生物量和花生四烯酸的高产率显示文摘以 1 .4cm和 2 .8cm光径的平板光生物反应器 ,恒定高光强 [2 4 0 0 μE/(m2 ·s) ],通气、无菌高密度连续培养雪地绿藻 (简称雪藻 )。连续采收并补充等量新鲜培养液 ,当藻体生物量(以去灰分干重表示 ,AFDW)达恒态时 ,研究雪藻比生长速率 (SGR)、藻体的生物产量、总脂肪酸 (TFA)和花生四烯酸 (AA)产率。结果表明 ,尽管随着比生长速率增加 ,雪藻高密度连续培养体系的AA/TFA、TFA/AFDW和AA/AFDW三个指标有不同程度的降低 ,但生物产量、总脂肪酸及其花生四烯酸产出都相当高。 2 .8cm光径的平板光生物反应器中 ,雪藻SGR为0 .2d- 1 时 ,每平方米光照面积日获 35 .3gAFDW的高产量 ;SGR 0 .1 35d- 1 时 ,TFA产率最大 ,为7.0g/(m2 ·d) ,此时AA产率也最高 ,达 2 .6g/(m2 ·d)。尽管 1 .4cm光径的反应器的培养体积比前者下降了 5 0 % ,但细胞浓度明显提高 ,单位光照面积的AFDW和TFA最大日产率只下降了37.3%和 1 0 % ,分别为 2 2 .1和 6.3g/(m2 ·d) ,而AA的最大日产率也是 2 .6g/(m2 ·d)。结果还表明 ,氮或磷营养亏缺能明显提高雪藻AA含量的各项指标 ,同时高光强也有利于雪藻累积AA。 | 刘建国 刘伟 Zvi Cohen Amos Richmond | 2002 | 海洋与湖沼2002，33，5： | 15 |
| 2 | FATTY ACIDS PROFILE IN A HIGH CELL DENSITY CULTURE OF ARACHIDONIC ACID-RICH PARIETOCHLORIS INCISA (TREBOUXIOPHYCEAE,CHLOROPHYTA) EXPOSED TO HIGH PFD显示文摘The changes in arachidonic acid (AA) and fatty acids profiles along the growth curve of Parietochloris incisa, a coccoid snow green alga, were studied in a 2.8 cm light-path flat photobioreactor, exposed to strong photon flux density [PFD, 2400 μEmol/(m 2·s)]. Sixteen fatty acids were identified by gas chromatography showing that AA was the dominant fatty acid (33%-41%) followed by linoleic acid (17%-21%). AA content was closely investigated with respect to total fatty acids (TFA), ash free dry weight (AFDW) of cell mass as well as total culture content. These parameters were influenced significantly in a similar manner by culture growth phase, i.e., slightly decreasing in the lag period, gradually increasing in the logarithmic phase, becoming maximal at the early stationary phase, starting to decrease at the late stationary phase, sharply dropping at the decline phase. The increase in AA per culture volume during the logarithmic phase was not only associated with the increase in AFDW but also connected with a corresponding increase in AA/TFA, TFA/AFDW as well as AA/AFDW. The sharp decrease in AA content of the culture during the decline phase was mainly due to the decrease in AA/TFA, TFA/AFDW and AA/AFDW, although AFDW declined only a small extent. Maximal AA concentration, obtained at the early stationary phase, was 900 mg/L culture volume, and the average daily net increase of AA during 9 days logarithmic growth was 1.7 g/(m 2·day). Therefore, harvesting prior to the decline phase in a batch culture, or at steady state in continuous culture mode seems best for high AA production. The latter possibility was also further confirmed by continuous culture with 5 gradients of harvesting rate. | 刘建国 Zvi Cohen 等 | 2002 | Chinese Journal of Oceanology and Limnology2002，20，2： | 9 |
| 3 | PHYSIOLOGICAL INHIBITORY EFFECT OF OCS IN ARACHIDONIC ACID-RICH PARIETOCHLORIS INCISA (TREBOUXIOPHYCEAE, CHLOROPHYTA)显示文摘Parietochloris incisa is an arachidonic acid rich snow green alga. The main physiological profiles, such as ash free dry weight (AFDW), chlorophyll, carotenoid, protein and total fatty acids (TFA), in this alga exposed to old culture supernatant (OCS) at the decline phase or its crude ethyl acetate extracts (CEAE) were investigated by using tubular photobioreactors of different diameters. Results showed that both OCS and CEAE had strong inhibitory effect on the above physiological parameters. The longer the culture was exposed to OCS and the more CEAE were added into the algal culture, the more the above physiological properties were inhibited. Arachidonic acid (AA), the dominant component of fatty acids in this alga, was also seriously inhibited with respect to total TFA, AFDW of cell mass, or culture volume, due to a probable reduction of enzymes activities catalyzing chain elongation from C18:1ω9 to AA. These results incontestably evidenced that some CEAE dissolving substances existing in OCS, like auto inhibitors, inhibited P. incisa growth through feedback. Hence, any efficient removal of auto inhibitors from algal culture to decrease their bioactivity could be good for maximal production of desired products like AA. | 刘建国 张成武 Zvi Cohen Amos Richmond | 2002 | Chinese Journal of Oceanology and Limnology2002，20，3： | 7 |
| 4 | Oxygen sufficiency controls TOP mRNA translation via the TSC-Rheb-mTOR pathway in a 4E-BP-independent manner显示文摘遇到 hypoxic 应力的房间由 downregulating 保存资源和精力蛋白质合成。这里我们 demonstratethat 在这回答的一机制是最高的 mRNAs 的翻译压抑编码 translationalapparatus 的部件。规定的这个模式包含 TSC 和 Rheb,当 Rheb 的 TSC1 或 TSC2 或 overexpression 的大美人在剥夺氧的房间救了最高的 mRNA 翻译。导致压力的翻译 repressionof 这些 mRNAs 密切与 4E-BP 的 hypophosphorylated 状态相关,一个翻译抑压者。然而, seriesof 4E-BP loss-of-function 和 gain-of-function 实验在氧或生长因素剥夺下面反驳在 phosphorylation statusof 4E-BP 和最高的 mRNAs 的翻译压抑之间的一种 cause-and-effect 关系。而且,血缺氧的 repressiveeffect 类似于由 Torin 1,而是更多 pronouncedthan 猛禽或 rictor 大美人由 mTOR 活动的有效抑制到达了那。同样,猛禽或 rictor 的缺乏它温和地 downregulated TOPmRNAs 的基础翻译效率,没能压制最高的 mRNAs 的调停氧的翻译激活。最后, TIA-1 和 TIAR co 击倒,二 RNA 有约束力的蛋白质以前在氨基的酸饥饿的房间在最高的 mRNAs 的翻译压抑含有, failedto 在另外的压力条件下面减轻最高的 mRNA 翻译。因此,近似翻译管理者 ofTOP mRNAs 的性质留下逃犯。 | Rachel Miloslavski Elad Cohen Adam Avraham Yifat I luz Zvi Hayouka Judith Kasir Rajini Mudhasani Stephen N. Jones Nadine Cybulski Markus A. Ruegg Ola Larsson Valentina Gandin Arjuna Rajakumar Ivan Topisirovic Oded Meyuhas | 2014 | Journal of Molecular Cell Biology2014，8，3： | 3 |
| 5 | Accumulation o f arachidonic acid-rich triacylglycerols in the microalga Parietochloris incis a (Trebuxiophyceae,Chlorophyta)显示文摘 | Inna Khozin-Goldberg Zvi Cohen | 2002 | Phytochemistry2002，，60： | 1 |
| 6 | Sonoablation and application of MRI guided focused ultrasound in a preclinical model显示文摘 | Zion Zibly Christian A. Graves Sagi Harnof Moshe Hadani Zvi R. Cohen | 2014 | Journal of Clinical Neuroscience2014，，： | 1 |
| 7 | Electronic circuit simulation in engineering education显示文摘 | Sam Ben-Yaakov Yuval Rot Yehuda A virav Alon Zvi Cohen | 1990 | EC Newsletter1990，12，2： | 1 |
| 8 | Influences of textile characteristics on the ten- sile properties of warp knitted cement based composites显示文摘 | Alva Peled Zvi Cohen | 2008 | Cement & Concrete Composites2008，30，： | 1 |
| 9 | Treatment of Chronic Hepatitis C Virus Infection via Antioxidants: Results of a Phase I Clinical Trial显示文摘 | Alla Melhem Mirela Stern Oren Shibolet Eran Israeli Zvi Ackerman Orit Pappo Nilla Hemed Mina Rowe Hana Ohana George Zabrecky Robert Cohen Yaron Ilan | 2005 | Journal of Clinical Gastroenterology2005，，8： | 1 |
| 10 | Mobilization of arachidonyl moieties from triacylglycerels into chloreplastic lipids following recovery from nitrogen starvation of the microalga Parietochloris incisa 显示文摘 | Khozin - Goldberg Irma Shrestha Pushkar Cohen Zvi | 2005 | Blochimica Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids2005，1738，1： | 1 |
| 11 | Brain Metastases in Patients with Ovarian Carcinoma: Prognostic Factors and Outcome显示文摘 | Zvi R. Cohen Dima Suki Jeffrey S. Weinberg Eric Marmor Frederick F. Lang David M. Gershenson Raymond Sawaya | 2004 | Journal of Neuro - Oncology2004，，3： | 1 |
| 12 | Optimal growth conditions for Isochrysis galbana显示文摘 | Drora Kaplan Zvi Cohen Aharon Abeliovich | 1994 | Journal of Biotechnology1994，37，2： | 1 |
| 13 | Differential response of microalgae to the substituted pyridazinone,sandoz 9785,reveal different pathways in the biosynthesis of eicosapentaenoic acid 显示文摘 | Inna Khozin Zvi Cohen | 1996 | Phytochemistry1996，42，4： | 1 |
| 14 | Role of Helicobacter pylori Infection in Complications from Meckel’s Diverticulum显示文摘 | Zvi Ackerman David Peston Patrizia Cohen | 2003 | Digestive Diseases and Sciences2003，，6： | 1 |
| 15 | Mobilization of arachidonyl moieties from triacylglycerols intochloroplastic lipids following recovery from nitrogen starvation of the microalga Parietochloris inc/sa显示文摘 | Inna Khozin-Goldberg Pushkar Shrestha Zvi Cohen | 2005 | Biochimica et Biophysica Acta2005，1738，： | 1 |
| 16 | Influences of textilecharacteristics on the tensile properties of warp knittedcement based composites显示文摘 | PELED Alva COHEN Zvi | 2008 | Cement&ConcreteComposites2008，，30： | 1 |
| 17 | Do children tolerate thoracoscopic sympathectomy better than adults?显示文摘 | Zvi Steiner Zahavi Cohen Oleg Kleiner Ibrahim Matar Jorge Mogilner | 2008 | Pediatric Surgery International2008，，3： | 1 |
| 18 | Biosynthesis of eicosapentaenoic acid in the microalgaPorphyridium cruentum. I: The use of externally supplied fatty acids显示文摘 | Devora Shiran Inna Khozin Yair M. Heimer Zvi Cohen | 1996 | Lipids1996，，12： | 1 |
| 19 | Production potential of eicosapentaenoic acid byMonodus subterraneus显示文摘 | Zvi Cohen | 1994 | Journal of the American Oil Chemists’ Society1994，，9： | 1 |
| 20 | Proximity clouds-an acceleration technique for 3D grid traversal显示文摘 | Daniel Cohen Zvi Sheffer | 1994 | The Visual Computer1994，11，1： | 1 |
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