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您的检索式：作者名="Changxu Song"
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| 1 | A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification显示文摘Background:Porcine reproductive and respiratory syndrome virus(PRRSV),and particularly its highly pathogenic genotype(HP-PRRSV),have caused massive economic losses to the global swine industry.Results:To rapidly identify HP-PRRSV,we developed a direct real-time reverse transcription polymerase chain reaction method(dRT-PCR) that could detect the virus from serum specimen without the need of RNA purification.Our dRT-PCR assay can be completed in 1.5 h from when a sample is received to obtaining a result.Additionally,the sensitivity of dRT-PCR matched that of conventional reverse transcription PCR(cRT-PCR) that used purified RNA.The lowest detection limit of HP-PRRSV was 6.3 TCID_(50) using dRT-PCR.We applied dRT-PCR assay to 144 field samples and the results showed strong consistency with those obtained by cRT-PCR.Moreover,the dRT-PCR method was able to tolerate 5-20%(v/v) serum.Conclusions:Our dRT-PCR assay allows for easier,faster,more cost-effective and higher throughput detection of HP-PRRSV compared with cRT-PCR methods.To the best of our knowledge,this is the first report to describe a real-time RT-PCR assay capable of detecting PRRSV in crude serum samples without the requirement for purifying RNA.We believe our approach has a great potential for application to other RNA viruses. | Kang Kang Keli Yang Jiasheng Zhong Yongxiang Tian Limin Zhang Jianxin Zhai Li Zhang Changxu Song Christine Yuan Gou Jun Luo Deming Gou | 2015 | Journal of Animal Science and Biotechnology2015，6，1： | 2 |
| 2 | Isolation and characteriza- tion of a potential biocontrol Brevibacillus laterosporus 显示文摘 | Zhen Song Kaiqi Liu Changxu Lu | 2011 | African Journal of Microbiology Research2011，5，18： | 1 |
| 3 | Mesh Force Modelling and Parametric Studies for Compound Oscillatory Roller Reducer显示文摘A compound oscillatory roller reducer(CORR)with a first-stage gear transmission and a second-stage oscillatory roller transmission is presented.The transmission principle of oscillatory roller transmission is introduced,and the tooth profile equation of the inner gear is derived.The analytical model of mesh force considering the installation errors and manufacturing errors is proposed.Then,parametric studies considering different errors on the mesh force are conducted.Results show that the design parameters are significant factors for mesh force.The mesh force is reduced by 17%as the eccentricity of disk cam increases from 2.5 mm to 4 mm.When the radius of the movable roller increases from 7 mm to 20 mm,the mesh force decreases by 8%.As the radius of disk cam increases from 125 mm to 170 mm,the mesh force is decreased by 26.5%.For the impacts of errors,the mesh force has a noticeable fluctuation when these errors exist including the manufacturing error of disk cam,the installation error of disk cam and the manufacturing error of movable roller change.The prototype of the reducer is manufactured and preliminary run-in test proved the feasibility of the transmission principle. | Changxu Wei Chaosheng Song Caichao Zhu Chengcheng Liang Long Hu | 2021 | Chinese Journal of Mechanical Engineering2021，34，4： | 0 |
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