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| 1 | Relationship of Helicobacterpylori eradication with gastric cancer and gastric mucosal histological changes: a 10-year follow-up study显示文摘 | Zhou Liya Lin Sanren Ding Shigang Huang Xuebiao Jin Zhu Cui Rongli Meng Lingmei Li Yuan Zhang Li Guo Changji Xue Yan Yan Xiu'e Zhang Jing | 2014 | Chinese Medical Journal2014，，8： | 28 |
| 2 | Genetic analysis and gene fine mapping for a rice novel mutant(rl_(9(t)))with rolling leaf character显示文摘Leaf shape is an important parameter for ideotype breeding in rice, and the rolling of leaf is also beneficial to efficient ripening of grains. This encourages the explorations of new genes that regulate leaf shape. In this study, genetic analysis and gene mapping were carried out for a novel rolling leaf mutant identified from japonica variety Zhonghua 11. The SSR marker analysis showed that the mutant was controlled by a single recessive gene (rl9(t)) lo- cated on chromosome 9. Fine mapping of the Rl9(t) locus was conducted with 30 new STS markers de- veloped around Rl9(t) anchored region based on the sequence diversity between Nipponbare and 93-11. The fine mapping necessitated the contruction of a PAC contig encompassing the Rl9(t) locus, which was delimited to a 42 kb region. This could therefore en- hance the cloning of the target gene in further stud- ies. | YAN Changjie YAN Song ZHANG Zhengqiu LIANG Guohua LU Jufei GU Minghong | 2006 | Chinese Science Bulletin2006，51，1： | 22 |
| 3 | A Simple CRISPR/Cas9 System for Multiplex Genome Editing in Rice显示文摘Generating mutants bearing multiple gene modifications is essential for determining the functions of gene family members with redundant functions,or for analyzing epistatic relationships in genetic pathways.Using conventional methods,mutants with multiple gene mutations are generated by several rounds of intercrossing plants carrying a single mutation and identification of the offspring.This process is both time- | Chun Wang Lan Shen Yaping Fu Changjie Yan Kejian Wang | 2015 | Journal of Genetics and Genomics2015，42，12： | 22 |
| 4 | Introgression of qPE9-1 allele,conferring the panicle erectness,leads to the decrease of grain yield per plant in japonica rice(Oryza sativa L.)显示文摘圆锥花序建筑学是仔细与产量有关形成。qPE9-1 基因被证明了广泛地在产量很高的米饭栽培变种开发被过去常,授与在装饰用的梨树米饭的直立的圆锥花序特性。最近, qPE9-1 成功地被克隆;然而,谷物产量上的基因效果还每直立的圆锥花序等位基因 qPE9-1 的植物是争论的。在现在的学习,低垂的圆锥花序父母 Nongken 57,带 qpe9-1 等位基因,被用作周期性父母到连续地回交到从双 haploid (DH ) 的一根典型直立的圆锥花序线人口(Wuyunjing 8/Nongken 57 ) ,它以前被显示带 qPE9-1 等位基因。因此,一双 near-isogenic 线(无) 被开发。在无之间的农学的特点的比较证明当 qpe9-1 被 qPE9-1 代替时,圆锥花序建筑学从低垂树立被改变;而且,圆锥花序长度,植物高度, 1000 谷物重量和 tillers 显著地被减少,因而在 30% 每植物导致谷物产量的戏剧的减少。因此,我们断定 qPE9-1 是控制圆锥花序建筑学的一个主要因素,并且 qPE9-1 有多种的自然,与每植物的谷物产量上的否定效果。这结果强烈建议直立的圆锥花序等位基因 qPE9-1 应该在产量很高的繁殖实践和另外的有利基因被使用。另外,吃并且煮质量上的 qPE9-1 的效果也在现在的学习被讨论。 | Xiuhua Yi Zujian Zhang Shengyuan Zeng Chunyan Tian Juncheng Peng Min Li Yue Lu Qingcai Meng Minghong Gu Changjie Yan | 2011 | Journal of Genetics and Genomics2011，38，5： | 20 |
| 5 | QTL editing confers opposing yield performance in different rice varieties显示文摘Grain yield is one of the most important and complex trait for genetic improvement in crops; it is known to be controlled by a number of genes known as quantitative trait loci(QTLs). In the past decade, many yield-contributing QTLs have been identified in crops.However, it remains unclear whether those QTLs confer the same yield performance in different genetic backgrounds. Here, we performed CRISPR/Cas_9-mediated QTL editing in five widely-cultivated rice varieties and revealed that the same QTL can have diverse, even opposing, effects on grain yield in different genetic backgrounds. | Lan Shen Chun Wang Yaping Fu Junjie Wang Qing Liu Xiaoming Zhang Changjie Yan Qian Qian Kejian Wang | 2018 | Journal of Integrative Plant Biology2018，60，2： | 18 |
| 6 | Development of gene-tagged molecular markers for starch synthesis-related genes in rice显示文摘Improving grain quality is an important goal in breeding new elite rice varieties,requiring effective tools for the identification of target genotypes.Molecular marker-aided selection(MAS),combined with conventional breeding approaches,enables us to pre-cisely identify the individual genotypes that are associated with different grain quality features,which can dramatically improve the breeding efficiency.However,to date,the number of molecular markers used in MAS for grain quality improvement is still somewhat limited.In this study,based on our previous study that rice grain quality is strongly associated with starch synthesis in the endosperm,we developed 51 gene-tagged molecular markers according to sequence variations in 18 starch synthesis-related genes from 16 typical rice cultivars.These markers can discriminate the different alleles among rice germplasms.These novel markers will provide effective tools in improving grain quality via the breeding new elite rice varieties. | TIAN ZhiXi YAN ChangJie QIAN Qian YAN Song XIE HuiLan WANG Fang XU JieFen LIU GuiFu WANG YongHong LIU QiaoQuan TANG ShuZhu LI JiaYang GU MingHong | 2010 | Chinese Science Bulletin2010，55，33： | 12 |
| 7 | Targeted mutagenesis of amino acid transporter genes for rice quality improvement using the CRISPR/Cas9 system显示文摘High grain protein content(GPC) reduces rice eating and cooking quality(ECQ). We generated OsAAP6 and OsAAP10 knockout mutants in three high-yielding japonica varieties and one japonica line using the CRISPR/Cas9 system. Mutation efficiency varied with genetic background in the T_0 generation, and GPC in the T_1 generation decreased significantly,owing mainly to a reduction in glutelin content. Amylose content was down-regulated significantly in some Osaap6 and all Osaap10 mutants. The increased taste value of these mutants was supported by Rapid Visco Analysis(RVA) profiles, which showed higher peak viscosity and breakdown viscosity and lower setback viscosity than the wild type. There were no significant deficiencies in agronomic traits of the mutants. Targeted mutagenesis of OsAAP6 and OsAAP10, especially OsAAP10, using the CRISPR/Cas9 system can rapidly reduce GPC and improve ECQ of rice, providing a new strategy for the breeding cultivars with desired ECQ. | Shiyu Wang Yihao Yang Min Guo Chongyuan Zhong Changjie Yan Shengyuan Sun | 2020 | The Crop Journal2020，8，3： | 11 |
| 8 | Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice显示文摘The clustered regularly interspaced short palindromic repeats(CRISPR)-associated endonuclease 9(CRISPR/Cas9) system has emerged as a promising technology for specific genome editing in many species. Here we constructed one vector targeting eight agronomic genes in rice using the CRISPR/Cas9 multiplex genome editing system. By subsequent genetic transformation and DNA sequencing, we found that the eight target genes have high mutation efficiencies in the T_0 generation. Both heterozygous and homozygous mutations of all editing genes were obtained in T_0 plants. In addition, homozygous sextuple, septuple, and octuple mutants were identified. As the abundant genotypes in T_0 transgenic plants, various phenotypes related to the editing genes were observed. The findings demonstrate the potential of the CRISPR/Cas9 system for rapid introduction of genetic diversity during crop breeding. | Lan Shen Yufeng Hua Yaping Fu Jian Li Qing Liu Xiaozhen Jiao Gaowei Xin Junjie Wang Xingchun Wang Changjie Yan Kejian Wang | 2017 | Science China(Life Sciences)2017，60，5： | 11 |
| 9 | Causal effects of shelter forests and water factors on desertification control during 2000–2010 at the Horqin Sandy Land region, China显示文摘The Horqin Sandy Land(HSL), the largest sandy land in the semi-arid agro-pastoral ecotone of Northeast China, has been subject to desertification during the past century. In response, and to control the desertification,government implemented the Three-North Shelter/Protective Forest Program, world's largest ecological reforestation/afforestation restoration program. The program began in1978 and will continue for 75 years until 2050. Understanding the dynamics of desertification and its driving forces is a precondition for controlling desertification.However, there is little evidence to directly link causal effects with desertification process(i.e., on the changing area of sandy land) because desertification is a complex process,that can be affected by vegetation(including vegetation cover and extent of shelter forests) and water factors such as precipitation, surface soil moisture, and evapotranspiration.The objectives of this study were to identify how influencing factors, especially shelter forests, affected desertification in HSL over a recent decade. We used Landsat TM imagery analysis and path analysis to identify the effects of spatiotemporal changes in water and vegetation parameters during2000–2010. Desertification was controlled during the study period, as indicated by a decrease in desert area at a rate of163.3 km2year-1and an increase in the area with reduced intensity or extent of desertification. Total vegetation cover in HSL increased by 10.6 % during the study period and this factor exerted the greatest direct and indirect effects on slowing desertification. The contribution of total vegetation cover to controlling desertification increased with the intensity of desertification. On slightly and extremely severe desertified areas, vegetation cover contributed 5 and 42 % of the desertification reduction, respectively. There were significant correlations between total vegetation cover and water conditions(i.e., evapotranspiration and precipitation)and the area of shelter forests(P \ 0.0001), in which water conditions and the existence of shelter forests contributed49.7 and 12.8 % to total vegetation cover, respectively. The area of shelter forests increased sharply due to program efforts, but only shrub forests had significant direct effects on reducing the area of desertification categorized as slightly desertified. The reason for the lack of direct effect of increased arbor forests(accounting for 95.3 % of the total increase in shelter forests) on reducing desertification might be that the selected arbor species were not suited to water conditions(low precipitation, high evapotranspiration) prevailing at HSL. The establishment of shelter forests aided control of desertification in the HSL region, but the effect was less than expected. Effective control of desertification in the HSL region or other similar sandy areas will require greater improvements in vegetation cover. In particular,shrub species should be selected for plantation with reference to their potential to survive and reproduce in the harsh climatic and weather conditions typical of desertified areas. | Qiaoling Yan Jiaojun Zhu Xiao Zheng Changjie Jin | 2015 | Journal of Forestry Research2015，26，1： | 5 |
| 10 | Expanding the scope of genome editing with SpG and SpRY variants in rice显示文摘Dear Editor,The clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9(CRISPR/Cas9)system,since it was excavated,has been rapidly developed and sparked a revolution in the genome editing field.In principle,CRISPR/Cas9 system relies on the recognition of specific loci on the genome,which is titled the protospacer adjacent motif(PAM).However,the canonical Streptococcus pyogenes Cas9(SpCas9)nuclease only recognizes NGG or NAG PAMs,rendering an inherent obstacle in amplifying the application of CRISPR/Cas9 technology. | Jun Ren Xiangbing Meng Fengyue Hu Qing Liu Yuexuan Cao Huiying Li Changjie Yan Jiayang Li Kejian Wang Hong Yu Chun Wang | 2021 | Science China(Life Sciences)2021，64，10： | 4 |
| 11 | Erratum to: Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice显示文摘The same figure was misused for the PCR/RE assay results of Gn1a and GW2 fragments in Figure 3,and the arrows in the graphicsal result of GW2 were not on the tape.The corrected Figure 3 is as follows. | Lan Shen Yufeng Hua Yaping Fu Jian Li Qing Liu Xiaozhen Jiao Gaowei Xin Junjie Wang Xingchun Wang Changjie Yan Kejian Wang | 2019 | Science China(Life Sciences)2019，62，9： | 1 |
| 12 | Brassinosteroid and gibberellin coordinate rice seed germination and embryo growth by regulating glutelin mobilization显示文摘Seed germination is the beginning of a new lifecycle,and involves many complex physiological and biochemical reactions including seed reserve mobilization in the endosperm and nutrient transport and reuse in the embryo.Although glutelin is a dominant storage protein in rice,its contribution to seed germination and its regulatory mechanisms are mostly unknown.Gibberellin (GA) and brassinosteroid (BR),two major growth-promoting phytohormones,also play positive roles in controlling seed germination.However,how GA and BR interact and coordinate seed germination and facilitate glutelin mobilization remains unclear.In the present study,biochemical and physiological analyses of seed germination indicated that both GA and BR promote seed germination and post-germination growth.Exogenous application of GA restored germination defects caused by BR deficiency or insensitivity.Proteomic and q RT-PCR results showed that the expression of several glutelin proteins and their encoding genes was induced by BR and GA in the embryo.Expression assays suggested that the increased accumulation of glutelin protein in the embryo was due to the accelerated degradation of glutelin by a cysteine proteinase (REP-1) in the endosperm.The breakdown of glutelin in the endosperm showed a strict positive correspondence with the length of the shoot.The GluA2 mutation led to reduced degradation rate of glutelin and defects in seed germination,and the promotion effect of GA on seed germination was weakened in the glua2mutant.In vitro culture assay of rice embryos showed that glutelin mobilization functioned downstream of the GA and BR pathways to promote shoot elongation.These findings suggest a mechanism that mediates crosstalk between BR and GA in co-regulating rice seed germination and embryo growth. | Min Xiong Lingyi Chu Qianfeng Li Jiawen Yu Yihao Yang Peng Zhou Yong Zhou Changquan Zhang Xiaolei Fan Dongsheng Zhao Changjie Yan Qiaoquan Liu | 2021 | The Crop Journal2021，9，5： | 1 |
| 13 | Chromosome location of semidwarf gene sd-t and sd-n in indica rice显示文摘Great achievements have been madein breeding of semidwarf varieties(indica) since 1960e. Resultsshowed that the dwarf traits widelyused at present were controlled by thesame recessive major gene, sd-1,thus we faced the potential danger oflosing genetic diversity. The low yieldof semidwarf varieties newly-bred indomestic or abroad may be related tothe narrow use of dwarf resource. Soit was important to explore, appraise, | LI Xin~((1))) Gu Minghong~(1)) Xu Jin-feng~(2)) YAN Changjie~(1)) LIANG Guohua~(1)) 1)Dept of Agronomy,Agri Coll,Yangzhou Univ ,Yangzhou 225009 2)Office of Guannan County Goverment,Guannan 222500,China | 2002 | Chinese Rice Research Newsletter2002，10，1： | 1 |
| 14 | Genomewide comparative phylogenetic and molecular evolutionary analysis of tubby-like protein family in Arabidopsis , rice, and poplar显示文摘 | Zefeng Yang Yong Zhou Xuefeng Wang Shiliang Gu Jianmin Yu Guohua Liang Changjie Yan Chenwu Xu | 2008 | Genomics2008，，4： | 1 |
| 15 | Genes coding for starch branching enzymes are major contributors to starch viscosity characteristics in waxy rice ( Oryza sativa L.)显示文摘 | Yuepeng Han Mingliang Xu Xingyan Liu Changjie Yan Schuyler S. Korban Xiulan Chen Minghong Gu | 2003 | Plant Science2003，，2： | 1 |
| 16 | Serum amyloid A may potentiate prothrombotic and proinflammatory events in acute coronary syndromes显示文摘 | Changjie Song Ying Shen Eric Yamen Kenneth Hsu Weixing Yan Paul K. Witting Carolyn L. Geczy S. Ben Freedman | 2008 | Atherosclerosis2008，，2： | 1 |
| 17 | ERECT PANICLE2 Encodes a Novel Protein That Regulates Panicle Erectness in Indica Rice显示文摘 | Zhu Keming Tang Ding Yan Changjie Chi Zhengchang Yu Hengxiu Chen Jianmin Liang Jiansheng Gu Minghong Cheng Zhukuan | 2010 | Genetics2010，，2： | 1 |
| 18 | Molecular mapping of two semidwarf genes in an indica rice variety Aitaiyin3(Oryza sativa L.)显示文摘Genetic analysis established that Aitaiyin3,a dwarf rice variety derived from a semidwarf cultivar Taiyin1,carries two recessive semidwarf genes.By using simple sequence repeat(SSR)markers,we mapped the two semidwarf genes,sd-1 and sd-t2 on chromosomes 1 and 4,respectively.Sd-t2 was thus named because the semidrawf gene sd-t has already been identified from Aitaiyin 2 whose origin could be traced back to Taiyin1.The result of the molecular mapping of sd-1 gene revealed it is linked to four SSR markers found on chromosome 1.These markers are:RM297,RM302,RM212,and OSR3 spaced at 4.7 cM,0 cM,0.8cM and 0 cM,respectively.Sd-t2 was found to be located on chromosome 4 using five SSR markers:two markers,SSR332 and RM1305 located proximal to sd-t2 are spaced 11.6 cM,3.8 cM,respectively,while the three distally located primers,RM5633,RM307,and RM401 are separated by distances of 0.4 cM,0.0 cM,and 0.4 cM,respectively. | Zhao Xiangqiang Liang Guohua Zhou Jingsong Yan Changjie Cao Xiaoying Gu Minghong | 2006 | Frontiers in Biology2006，1，2： | 0 |
| 19 | Rapid improvement of rice eating and cooking quality through gene editing toward glutelin as target显示文摘Rice eating and cooking quality(ECQ)is a major concern of breeders and consumers,determining market competitiveness worldwide.Rice grain protein content(GPC)is negatively related to ECQ,making it possible to improve ECQ by manipulating GPC.However,GPC is genetically complex and sensitive to environmental conditions;therefore,little progress has been made in traditional breeding for ECQ.Here,we report that CRISPR/Cas9-mediated knockout of genes encoding the grain storage protein glutelin rapidly produced lines with downregulated GPC and improved ECQ.Our finding provides a new strategy for improving rice ECQ. | Yihao Yang Ziyan Shen Youguang Li Chenda Xu Han Xia Hao Zhuang Shengyuan Sun Min Guo Changjie Yan | 2022 | Journal of Integrative Plant Biology2022，64，10： | 0 |
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