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| 1 | Evaluation of rabbit antibody response against 8 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus显示文摘Objective:To immunize rabbits with 12 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus(E.granulosus) and measuring polyclonal antibody and humoral immune response using ELISA and gel diffusion.Methods:Two mentioned antigens were cloned and expressed in expression vector and purified by affinity chromatography.Four young rabbits were selected and challenged intradermallt with yielded recombinant antigens.Rabbits’ sera were collected post infection and were tested using ELISA and gel diffusion for polyclonal antibody detection 10 days after last injection.Results:The specific antibody against the recombinant peptides was efficiently produced within 4 weeks post infection.Conclusions: Produced recombinants proteins could induce the immune response of the rabbits successfully. This process might improve the clarification of diagnosis and vaccination as regards hydatidosis. | Jahangir Abdi Bahram Kazemi Mohammad Hasan Karimfar Mohammad Bagher Rokni | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，5： | 3 |
| 2 | Molecular cloning of virB12 gene of Brucella melitensis 16M strain in pET28a vector显示文摘Objective:To clone the virB12 gene in pET28a expression vector for production of recombinant protein to be used as antigenic component for future serological test development.Methods: Brucella melitensis(B.melitensis) 16M strain was cultured and bacterial DNA was extracted by Bioneer AccuPrep~ Genomic DNA Extraction Kit.Oligonucleotide primer pair was designed based on Brucella virB12 gene sequence with BamHI and HindIII restriction site at 5’ end of the forward and reverse primers,respectively.DNA amplification was performed using PrimSTAR~ HS DNA polymerase and the PCK product was purified by DNA AccuPrepGel Purification Kit.Purified DNA was cloned into pJET1.2 cloning vector.VirB12 gene fragment was excised from pJET1.2 asing BamHI/HindIII and subsequendy subcloned into pET28a(+).Results:Brucella virB12 gene was successfully cloned in pJET1.2 and then in pET28a(+) plasmids.PCR and restriction enzyme digestion confirms the procedure.Conclusion:We cloned and expressed the Brucella virB12 gene which could be used as antigenic component for specific serological assay development. | Shiva Mirkalantari Nour Amirmozafari Bahram Kazemi Gholamreza Irajian | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，7： | 2 |
| 3 | Evaluation of PCR assay in diagnosis and identification of cutaneous leishmaniasis: a comparison with the parasitological methods显示文摘 | Farideh Shahbazi Saed Shahabi Bahram Kazemi Mehdi Mohebali Ali Reza Abadi Zabiholah Zare | 2008 | Parasitology Research2008，，5： | 2 |
| 4 | Influence of random heterogeneity of shear wave velocity on sliding mass response and seismic deformations of earth slopes显示文摘Soil shear wave velocity has been recognized as a governing parameter in the assessment of the seismic response of slopes.The spatial variability of soil shear wave velocity can influence the seismic response of sliding mass and seismic displacements.However,most analyses of sliding mass response have been carried out by deterministic models.This paper stochastically investigates the effect of random heterogeneity of shear wave velocity of soil on the dynamic response of sliding mass using the correlation matrix decomposition method and Monte Carlo simulation(MCS).The software FLAC 7.0 along with a Matlab code has been utilized for this purpose.The influence of statistical parameters on the seismic response of sliding mass and seismic displacements in earth slopes with different inclinations and stiffnesses subject to various earthquake shakings was investigated.The results indicated that,in general,the random heterogeneity of soil shear modulus can have a notable impact on the sliding mass response and that neglecting this phenomenon could lead to underestimation of sliding deformations. | Pourya Kazemi Esfeh Bahram Nadi Nicholas Fantuzzi | 2020 | Earthquake Engineering and Engineering Vibration2020，19，2： | 2 |
| 5 | Comparison Between Covered and Bare Cheatham - Platinum Stents for Endovascular Treatment of Patients With Native Post-Ductal Aortic Coarctation显示文摘 | Bahram Sohrabi Peiman Jamshidi Alireza Yaghoubi Afshin Habibzadeh Yashar Hashemi-aghdam Araz Moin Babak Kazemi Samad Ghaffari Mohammad Reza Abdolahzadeh Baghayi Khalil Mahmoody | 2013 | JACC: Cardiovascular Interventions2013，，： | 1 |
| 6 | Gastroenterology显示文摘 | Seyed Mohammadreza Safavi Bahram Kazemi Mostafa Esmaeili | 2008 | Lasers Med Sci2008，23，： | 1 |
| 7 | Effects of low-level He–Ne laser irradiation on the gene expression of IL-1β, TNF-α, IFN-γ, TGF-β, bFGF, and PDGF in rat’s gingiva显示文摘 | Seyed Mohammadreza Safavi Bahram Kazemi Mostafa Esmaeili Alireza Fallah Alireza Modarresi Maziar Mir | 2008 | Lasers in Medical Science2008，，3： | 1 |
| 8 | Comparison of loop-mediated isothermal amplification (LAMP) and nested-PCR assay targeting the RE and B1 gene for detection of Toxoplasma gondii in blood samples of children with leukaemia显示文摘 | Shirzad Fallahi Seyyed Javad Seyyed Tabaei Yadollah Pournia Nozhat Zebardast Bahram Kazemi | 2014 | Diagnostic Microbiology & Infectious Disease2014，，： | 1 |
| 9 | Mesenchymal stem cells from trabecular meshwork become photoreceptor-like cells on amniotic membrane显示文摘 | Samad Nadri Shahin Yazdani Ehsan Arefian Zahra Gohari Mohamadreza Baghaban Eslaminejad Bahram Kazemi Masoud Soleimani | 2013 | Neuroscience Letters2013，，： | 1 |
| 10 | High yield of cells committed to the photoreceptor-like cells from conjunctiva mesenchymal stem cells on nanofibrous scaffolds显示文摘 | Samad Nadri Bahram Kazemi Mohamadreza Baghaban Eeslaminejad Shahin Yazdani Masoud Soleimani | 2013 | Molecular Biology Reports2013，，6： | 1 |
| 11 | Effects of low - level He - Ne laser irradiation on the gene expression of IL - 1β, TNF - α,IFN - γ, TGF - β, bFGF, and PDGF in rat's gingiva显示文摘 | Safari Seyed Mohammadreza Kazemi Bahram Esmaeili Mostafa | 2008 | Lasers Med Sci2008，23，： | 1 |
| 12 | First molecular identification of Leishmania species in a new endemic area of cutaneous leishmaniasis in Lorestan,Iran显示文摘Objective:To identify Leishman{u using PCR.Methods:This studs was conducted from April2009 to March 2011 in order to identify Leishmania species in a new endemic area of CL in Lorestan.Iran.Samples were taken from 62 patients that referred to the health centers in different cities of Lorestan province,the presence of Leishmcania was confirmed using direct smear and then grown in NNN media and mass cultured in RPM!1640 medium supplemented with 10%heat-inactivated fetal bovine serum.DNA was extracted from cultured promastigotes and used in P15-PCR.Results:45(72.6%)samples out of 62 showed a hand in the range of 485 hp and 17(27.4%)with a hand in the range of 626 hp which were similar to standard strains of Leichmania tropica(L.tropical and Leishnrania major(L.major),respectively.50(65.80%)of samples were collected from people with no history of travel in at least a year prior to the onset which shows that indigenous source of infection.Conclusions:Since the vector and reservoir of the two species are different.so precise and extensive control and prevention methods should be designed and earned out. | Farnaz Kheirandish Ali Chegeni Sharafi Bahram Kazemi Mojgan Bandehpour Mohamad javad Tarahi Ali Khamesipour | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，9： | 0 |
| 13 | Cytokine changes and embryo attachment in mouse endometrial cells following treated with peripheral blood mononuclear cells(PBMCs)expressing ectopic hCG,and hCG-activated PBMCs显示文摘Objective:To compare the effect of human chorionic gonadotropin(hCG)-producing peripheral blood mononuclear cells(PBMCs)and PBMCs activated by hCG in vitro and expressions of related immune genes in mouse implantation.Methods:hCG-producing PBMCs(transfected PBMC)and PBMCs activated by hCG in vitro were introduced into isolated mouse endometrial cells,while cell cultures were divided into four groups:the control,PBMC,transfected,and activated PBMC groups.The expression of studied genes(IL-1β,IL-6,Lif,and Vegf)was evaluated and blastocyst attachment on the cocultured cells(isolated endometrial cells and PBMC cells)was monitored in all four groups.Results:Data showed that expression decreased in the PBMC group compared to the treated PBMC(transfected and activated PBMCs)and increased in transfected PBMC compared to the activated PBMC.Attachment and migration of blastocysts were dramatically enhanced in the transfected PBMC group compared to the activated PBMC group(P<0.05).Conclusions:Use of hCG-producing PBMCs(transfected PBMC)has more influence on endometrial receptivity. | Delsuz Rezaee Mojgan Bandehpour Bahram Kazemi Saiyad Bastaminejad Sajad Najafi Mohammad Salehi | 2023 | Asian pacific Journal of Reproduction2023，12，2： | 0 |
| 14 | Cloning and expression of Neisseria meningitides luxS gene显示文摘Neisseria meningitides is a Gram-negative bacterium which is an important causative agent of septicemia and meningitis.Numerous pathogenic bacteria contain luxS,which is required for autoinducer-2 production.Neisseria meningitides contains a functional copy of luxS that is necessary for full meningococcal virulence.Neisseria meningitides DNA was extracted and its luxS gene was amplified by nested PCR.PCR product was purified and cloned in to pQE-30 expression vector.Recombinant plasmid was transformed and mass cultured.luxS was expressed in E.coli and confirmed by western blot analysis.In this study,N.meningitides luxS was amplified, cloned and expressed successfully.The sequencing of PCR product confirmed that amplified gene was luxS. Gene was expressed and observed in SDS-PAGE.Protein was reacted by his tag monoclonal antibody through western blot analysis. | Bahram Kazemi Nazila Baghalzadeh-Mohammadi Reza Hadavi Mojgan Bandehpour Elham Ghayour Majid Moghbeli Kazem Parivar | 2008 | Asian Pacific Journal of Tropical Medicine2008，1，2： | 0 |
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