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| 1 | 不同肥料对退化茶园根际土壤微生物代谢活性和群落结构的影响显示文摘于福建省安溪县桃舟乡,选择宿根连作20年茶园进行田间试验。以未施肥(CK)、氮肥(NF)、羊粪(GM)及豆科绿肥(LF)等处理后的茶树根际土壤作为供试土样,分析不同肥料对茶树根际土壤理化特性、微生物代谢活性及其不同类型磷脂脂肪酸含量变化等影响。结果表明:NF、GM和LF均能够提高茶园土壤pH值和有机质(OM)及速效钾(AK)等含量,其pH分别提高0.47%、2.36%和2.13%;OM分别提高17.56%、27.48%和54.96%;AK分别提高56.10%、63.41%和73.17%;碱解氮(AN)和速效磷(AP)在NF处理下分别下降了9.10%和335.00%;在GM处理下分别提高了17.06%和2.11倍;在LF处理下分别提高了16.70%和3.88倍;同时,施肥能提高土壤脲酶、蔗糖酶、多酚氧化酶和过氧化物酶等活性,且提高效果由高到底依次为LF、GM、NF;酸性磷酸酶活性(ACP)和微生物对磷素利用率(UTP)在NF处理下分别降低70.04%和249.00%;在GM处理下分别提高了24.74%和1.80倍;在LF处理下分别提高34.28%和2.51倍;与此同时,GM和LF还能够提高根际土壤微生物群落丰度以及菌群多样性。综上所述,豆科绿肥和羊粪能够有效改善茶园土壤肥力,促进根际微生物对养分的代谢活性,对提升茶园经济效益和生态效益具有现实意义。 | 蒋宇航 林生 林伟伟 陈婷 Yasir Arafat 位小丫 林文雄 | 2017 | 生态学杂志2017，36，10： | 15 |
| 2 | Extraction and RP-HPLC determination of taxol in rat plasma, cell culture and quality control samples显示文摘A rapid,sensitive,selective and validated reverse phase high-performance liquid chromatography(RP-HPLC)method for the estimation of paclitaxel in micro-sample of rat plasma and in culture of cancer cells was performed in this study.The mobile phase consisted of an optimized mixture of methanol:water:trifluroacetic acid(80:20:0.1,v/v/v).Column elution at a flow rate of 1 mL/minute with UV detection at 225 nm at room temperature was used.The RP-HPLC method was successfully applied for the determination of paclitaxel in plasma samples and in culture of cancer cells with nano-quantity of estimation.The validation studies were performed in accordance with the International Conference on Harmonization(ICH)guidelines.The intra-and inter-day precision showed that the coefficients of variation ranged from 1.07%to 4.27%at different levels of concentrations.To the best of our knowledge,this study also reported for the first time the optimization of different solvents for effective extraction of paclitaxel wherein tert.-butyl methyl ether(TBME):diethyl ether(DEE)in 50:50 v/v composition was found most efficient with extraction efficiency ranging between 77.99%and 91.74%and between 76.14 and 93.66%in the plasma and cell culture,respectively.This proposed method was successfully applied to study the pharmacokinetics of paclitaxel and the influence of verapamil and all-trans retinoic acid(atRA)on paclitaxel pharmacokinetics in rat models.This proposed method might emerge as a valuable aid in the laboratory monitoring of paclitaxel in a variety of in vitro as well as in vivo scenarios. | Rakesh Kumar Tekade Antony D'Emanuele Abdelbary Elhissi Ashish Agrawal Anurekha Jain Basel Tawfiq Arafat Narendra Kumar Jain | 2013 | The Journal of Biomedical Research2013，27，5： | 5 |
| 3 | Randomized Clinical Trial of Cutting Balloon Angioplasty versus High-Pressure Balloon Angioplasty in Hemodialysis Arteriovenous Fistula Stenoses Resistant to Conventional Balloon Angioplasty显示文摘 | Syed Arafat Aftab Kiang Hiong Tay Farah G. Irani Richard Hoau Gong Lo Apoorva Gogna Benjamin Haaland Seck Guan Tan Siew Png Chng Shanker Pasupathy Hui Lin Choong Bien Soo Tan | 2013 | Journal of Vascular and Interventional Radiology2013，，： | 2 |
| 4 | Sep Sci Technol 显示文摘 | Arafat H A Ahnert F Pinto N G | 2004 | 39(1): 43-622004，39，1： | 1 |
| 5 | Non-linear interactions in imperfect beams at veering显示文摘 | Walter Lacarbonara Haider N. Arafat Ali H. Nayfeh | 2005 | International Journal of Non-Linear Mechanics2005，，7： | 1 |
| 6 | Effect of chemical surface heterogeneity on the adsorption mechanism of dissolved aromatics on activated carbon显示文摘 | Marcus Franz Hassan A Arafat Neville G Pinto | 2000 | Carbon2000，38，： | 1 |
| 7 | Effect of arbuscular mycorrhizal fungi on growth,mineral nutrition,antioxidant enzymes activity and fruit yield of tomato grown under salinity stress显示文摘 | ARAFAT A L HE C | 2011 | Scientia Horticulturae2011，127，3： | 1 |
| 8 | Effect of chemical surface heterogeneity on the adsorption mechanism of dissolved aromatics on activated carbon 显示文摘 | Franz M Arafat H A Pinto N G | 2000 | Carbon2000，35，13： | 1 |
| 9 | Modal interations in the vibration of a heated annular plate 显示文摘 | Arafat H N Neyfeh A H | 2004 | International Journal of Nonlinear Mechanics2004，39，10： | 1 |
| 10 | A high normal TSH is associated with the metabolic syndrome显示文摘 | Ruhla S Weickert MO Arafat AM | 2010 | Clin Endocrinol (Oxf)2010，72，5： | 1 |
| 11 | Anti-metastatic and antitumor growth effects of Origanum majorana on highly metastatic human breast cancer cells:inhibition of NFκB signaling and reduction of nitric oxide production显示文摘 | Al Dhaheri Y Attoub S Arafat K | 2013 | PLo S One2013，8，68： | 1 |
| 12 | Ultrafiltration of polysaccharide-protein mixtures: elucidation of fouling mecltanisms and fouling control by membrane surface modification显示文摘 | SUSANTO H ARAFAT H JANSSEN E M L | 2008 | Separation and Purification Technology2008，63，： | 1 |
| 13 | Effect of chemical surface heterogeneity on the adsorption mechanism of dissolved aromatics on activated carbon 显示文摘 | Marcus Franz A Arafat Hassan G Pinto Neville | 2000 | Carbon2000，38，13： | 1 |
| 14 | Chronic administration of the selective P2X3, P2X2/3 receptor antagonist, A-317491, transiently attenuates cancer-induced bone pain in mice显示文摘 | Rikke Rie Hansen Arafat Nasser Sarah Falk Signe B. Baldvinsson Pernille H. Ohlsson Justyna M.C. Bahl Michael F. Jarvis Ming Ding Anne-Marie Heegaard | 2012 | European Journal of Pharmacology . 2012 (1-3)2012，，： | 1 |
| 15 | The use of remote sensing and GIS for the estimation of water loss from Tushka lakes, southwestern desert , Egypt 显示文摘 | Bastawesy M A Khalaf F I Arafat S M | 2008 | African Earth Sciences2008，，52： | 1 |
| 16 | An adenovirus encoding proapoptotic Bax induces apoptosis and enhances the radiation effect in human ovarian cancer显示文摘 | Arafat WO Comez-Navano J Xiang J Barnes MN Mahasreshti P Alvarez RD | 2000 | Mol Ther2000，1，： | 1 |
| 17 | Hybrid repair of aortic aneurysm in patients with previous coarctation 显示文摘 | Idrees J Arafat A Svensson LG | 2013 | J Thorac Cardiovasc Surg2013，3，： | 1 |
| 18 | Osteopontin protects the islets and beta-cells from interleukin-1beta-mmediated cytotoxicity through negative feedback regulation of nitric oxide显示文摘 | Arafat HA Katakam AK Chipitsyna G | 2007 | Endocrinology2007，148，2： | 1 |
| 19 | Occupational burnout levels in emergency medicine-a stage 2 nationwide study and analysis显示文摘 | Popa F Arafat R Purcarea V L | 2010 | J Med Life2010，3，3： | 1 |
| 20 | A Simple Time Domain Algorithm for Detection of Ventricular Fibrillation in Electrocardiogram显示文摘 | Arafat M A Chowdhury A W Hasan M K | | 0，，03： | 1 |
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