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| 1 | The Cooperative Activities of CSLD2, CSLD3, and CSLD5 Are Required for Normal Arabidopsis Development显示文摘Glycosyltransferases of the Cellulose Synthase Like D (CSLD) subfamily have been reported to be involved in tip growth and stem development in Arabidopsis. The csld2 and csld3 mutants are root hair defective and the csld5 mutant has reduced stem growth. In this study, we produced double and triple knockout mutants of CSLD2, CSLD3, and CSLD5. Unlike the single mutants and the csld2/csld3 double mutant, the csld2/csld5, csld3/csld5, and csld2/ csld3/csld5 mutants were dwarfed and showed severely reduced viability. This demonstrates that the cooperative activities of CSLD2, CSLD3, and CSLD5 are required for normal Arabidopsis development, and that they are involved in important processes besides the specialized role in tip growth. The mutant phenotypes indicate that CSLD2 and CSLD3 have overlapping functions with CSLD5 in early plant development, whereas the CSLD2 and CSLD3 proteins are non-redundant. To determine the biochemical function of CSLD proteins, we used transient expression in tobacco leaves. Microsomes containing heterologously expressed CSLD5 transferred mannose from GDP–mannose onto endogenous acceptors. The same activity was detected when CSLD2 and CSLD3 were co-expressed but not when they were expressed separately. With monosaccharides as exogenous acceptors, microsomal preparations from CSLD5-expressing plants mediated the transfer of mannose from GDP–mannose onto mannose. These results were supported by immunodetection studies that showed reduced levels of a mannan epitope in the cell walls of stem interfascicular fibers and xylem vessels of the csld2/csld3/csld5 mutant. | Lan Yin Yves Verhertbruggen Ai Oikawa Chithra Manisseri Bernhard Knierim Lina Prak Jacob Kriiger Jensen Paul Knox Manfred Auer William G.T. Willatsa and Henrik Vibe Scheller | 2011 | Molecular Plant2011，4，6： | 2 |
| 2 | Inhibition of delta-like-4-mediated signaling impairs reparative angiogenesis after ischemia显示文摘 | AI Hai Zen A Oikawa A Bazan-Peregrino M | 2010 | Circ Res2010，107，2： | 1 |
| 3 | The circuit of Papez in mesial temporal sclerosis: MRI显示文摘 | Oikawa H Sasaki M Tamakawa Y et aI | 2001 | Neuroradiology2001，43，： | 1 |
| 4 | Inactivation of OslRX10 Leads to Decreased Xylan Content in Rice Culm Cell Walls and Improved Biomass Saccharification显示文摘Dear Editor, Xylan polysaccharides constitute the major non-cellulosic components in secondary cell walls of dicots and in both primary and secondary cell walls of grasses (Scheller and Ulvskov,2010).Xylan is composed of a linear backbone of 13(1-4)-linked xylose (Xyl).In grasses,the xylan backbone is substituted with O3-1inked and O2-1inked arabinose residues and,to a lesser extent,α(1-3)-Iinked glucuronic acid residues.A unique feature of grass xylans is the presence of hydroxycinnamate esters (ferulic and p-coumaric acid residues)attached to C5 of some of the arabinosyl residues (Carpita,1996;Vogel,2008;Scheller and Ulvskov,2010).Xylan contributes to the recalcitrance of plant cell walls to enzymatic degradation probably through direct interaction with cellulose microfibrils and also through formation of ferulate cross-links (liyama et al.,1994;Yin et al.,2011). | Xuewei Chen Miguel E. Vega-Sanchez Yves Verhertbruggenb Dawn Chiniquy Patrick E. Canlas Alexandra Fagerstrom Lina Prak Ulla Christensen Ai Oikawa Mawsheng Chern Shimin Zuo Fan Lin Manfred Auer William G.T. Willats Laura Bartley Jesper Harholt Henrik V. Scheller Pamela C. Ronald | 2013 | Molecular Plant2013，6，2： | 1 |
| 5 | Comparison of orthodontic treatment outcomes in adults with skeletal open bite between conventional edgewise treatment and implant-anchored orthodontics 显示文摘 | Deguchi T Kurosaka H Oikawa H et aI | 2011 | Am J Orthod Dentofacial Orthop2011，139，4： | 1 |
| 6 | Complex Regulation of Prolyl-4-Hydroxylases Impacts Root Hair Expansion显示文摘根头发是由尖端生长,与花粉试管分享的一个过程,轴突,和真菌的 hyphae 发展的单个房间。然而,结构的植物细胞墙强加限制完成尖端生长。除了多糖,植物房间墙由 hydroxyproline 富有的 glycoproteins (HRGP ) 组成,它包括几组 O-glycoproteins,包括 extensins (EXT ) 。脯氨酸 hydroxylation, HRGP 的早 translational 以后修正(PTM ) 由 prolyl 4-hydroxylases (P4Hs ) 催化,定义他们的随后的 O-glycosylation 地点。在这个工作,我们的基因分析证明那 P4H5,并且到更小的程度 P4H2 和 P4H13,为根头发尖端生长是枢轴的。第二,我们证明 P4H5 把 invitro 比较喜欢为 EXT 底层而非为另外的 HRGP 的特性。第三由交换途径的 P4H 倡导者和蛋白质, weshow 那 P4H2 和 P4H13 有可互换的功能,但是不能代替 P4H5。这三 P4Hs 被显示被指向到能分泌的小径,在 P4H5 与 P4H2 和 P4H13 形成 dimers 的地方。最后,我们在房间墙建筑学上探索缺乏的脯氨酸 hydroxylation 的影响。一起拿,我们的结果支持在哪个在 EXT 上改正 peptidyl 脯氨酸 hydroxylation 的一个模型,并且可能在另外的 HRGP,在 Arabidopsis thaliana 为自己组装的合适的房间墙和因此根头发延伸被要求。 | Silvia M. Velasauez Martiniano M. Ricardi Christian Peter Poulsen Ai Oikawa Adiphol Dilokpimol Adnan Halim Silvina Mangano Silvina Paola Denita Juarez Eliana Marzol Juan D. Salgado Salter Javier Gloazzo Dorosz Cecilia Borassi Svenning Rune Moller Rafael Buono Yukiko Ohsawa Ken Matsuoka Marisa S. Otegui Henrik V. Scheller Naomi Geshi Bent Larsen Petersen Norberto D. lusem Jose M. Estevez | 2015 | Molecular Plant2015，8，5： | 0 |
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