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您的检索式：作者名="ASHRAF Javaria"
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| 1 | 球孢白僵菌对柑橘木虱内共生菌的影响显示文摘为明确球孢白僵菌[Beauveria bassiana(Balsamo)]侵染柑橘木虱后其体内共生菌的变化,研究虫生真菌、柑橘木虱及内共生菌三者间的互作关系,采用荧光原位杂交技术(FISH),测定2个球孢白僵菌(FAFU-12和FAFU-16)菌株亚致死浓度(LC_(25))对柑橘木虱F_(1)代内共生菌Wolbachia和Carsonella的影响。结果表明,与对照相比,FAFU-12和FAFU-16处理组中柑橘木虱F1代2种内共生菌红色荧光分布范围减少,荧光强度减弱。球孢白僵菌亚致死剂量处理改变了柑橘木虱F_(1)代内共生菌Wolbachia和Carsonella的含量及分布,2种内共生菌参与了虫生真菌侵染宿主致病过程中的互作。 | 戴佳旺 王会停 崔施施 方元 徐宏涛 赖文昶 杨晨 李小芳 李新安 HAFIZA Javaria Ashraf 王联德 | 2021 | 武夷科学2021，37，2： | 0 |
| 2 | Improved Gossypium raimondii genome using a Hi-C-based proximity-guided assembly显示文摘Introduction:Genome sequence plays an important role in both basic and applied studies.Gossypium raimondii,the putative contributor of the D subgenome of upland cotton(G.hirsutum,highlights the need to improve the genome quality rapidly and efficiently.Methods:We performed Hi-C sequencing of G.raimondii and reassembled its genome based on a set of new Hi-C data and previously published scaffolds.We also compared the reassembled genome sequenee with the previously published G raimondii genomes for gene and genome sequence collinearity.Result:A total of 9842%of scaffold sequences were clustered successfully,among which 99.72%of the clustered sequences were ordered and 99.92%of the ordered sequences were oriented with high-quality.Further evaluation of results by heat-map and collinearity analysis revealed that the current reassembled genome is significantly improved than the previous one(Nat Genet 44:98-1103,2012).Conclusion:This improvement in G raimondii genome not only provides a better reference to increase study efficiency but also offers a new way to assemble cotton genomes.Furthermore,Hi-C data of G.raimondii may be used for 3D structure research or regulating analysis. | YANG Qiuhong ZUO Dongyun CHENG Hailiang ZHANG Youping WANG Qiaolian JAVARIA Ashraf FENG Xiaoxu LI Simin CHEN Xiaoqin LIU Shang SONG Guoli | 2021 | Journal of Cotton Research2021，4，3： | 0 |
| 3 | Map-based cloning of a recessive gene v_1 for virescent leaf expression in cotton(Gossypium spp.)显示文摘Background: Virescence, as a recognizable phenotype in the early development stage of cotton, is not only available for research on chloroplast development and photosynthesis but also for heterosis exploitation in cotton.Methods: In current study, for fine mapping of virescent-1(V_1) in cotton, three populations with a total of 5 678 individuals were constructed using T582 which has the virescent trait. Tobacco rattle virus, TRV1 and TRV2(pYL156),were used as vectors for the virus-induced gene silencing(VIGS) assay.Results: The v_1 gene was fine-mapped to a 20 kb interval on chromosome 20 of tetraploid cotton. We identified only one candidate gene with four single nucleotide polymorphisms between parents, among which the single nucleotide polymorphism at the position of 1 082 base pair caused the change of amino acid residue from Arg(3-79) to Lys(T582). The relative expression of the candidate gene in virescent plants was extensively lower than that in normal plants. Nullification of the gene by VIGS significantly turned the green leaf of normal cotton plants into yellow. We named this candidate gene as GhRVL.Conclusions: This study will facilitate the further research on virescent formation, and will be useful for breeding of hybrid cottons. | ZHANG Youping WANG Qiaolian ZUO Dongyun CHENG Hailiang LIU Ke ASHRAF Javaria LI Simin FENG Xiaoxu YU John Z. SONG Guoli | 2018 | Journal of Cotton Research2018，1，2： | 0 |
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