| 3 | Transformation of Coat Protein Encoding Gene from Soil-Borne Mosaic Virus into Wheat显示文摘CWMV-CP1 target gene and bar selection gene were co-transferred into commercial wheat vari-ety of Yangmai158 by particle bombardment. In total, 145 resistant plants to 3 - 5 mg L-1 Bialaphos were ob-tained, 21 plants were identified to be positive in T0 generation by PCR-Southern test, and the transformationfrequency had 0. 99%. T1 plants were further tested by PCR and Southern hybridization. Results demonstra-ted that the alien resistance gene had been integrated into the wheat genome. The segregation ratio of CP1+ toCP1- in T1 generation was 1.0 to 1.3, and didn't agree with Mendelian rule. RT-PCR result from T2 plantsshowed that the alien gene CWMV-CP1 had stable expression in wheat genetic background. | PANGJun-lan XUHui-jun DULi-pu YEXing-guo LILian-cheng XINZHi-yong MAYou-zhi DIAOAi-po AdamsMJ | 2003 | Agricultural Sciences in China2003，2，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员