|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Potential one-step strategy for PET degradation and PHB biosynthesis through co-cultivation of two engineered microorganisms显示文摘The management and recycling of plastic waste is a challenging global issue.Polyethylene terephthalate(PET),one of the most widely used synthetic plastics,can be hydrolyzed by a series of enzymes.However,upcycling the resulting monomers is also a problem.In this study,we designed a co-cultivation system,in which PET degradation was coupled with polyhydroxybutyrate(PHB)production.First,PETase from Ideonalla sakaiensis was expressed in Yarrowia lipolytica Po1f with a signal peptide from lipase.The engineered PETase-producing Y.lipolytica was confirmed to hydrolyze bis(2-hydroxyethyl)terephthalate(BHET)and PET powder into the monomers terephthalate(TPA)and ethylene glycol(EG).Simultaneously,a TPA-degrading Pseudomonas stutzeri strain isolated from PET waste was transformed with a recombinant plasmid containing the phb CAB operon from Ralstonia eutropha,which encodes enzymes for the biosynthesis of PHB.The two co-cultivated engineered microbes could directly hydrolyze BHET to produce the bioplastic PHB in one fermentation step.During this process,5.16 g/L BHET was hydrolyzed in 12 h,and 3.66 wt%PHB(3.54 g/L cell dry weight)accumulated in 54 h.A total of 0.31g/L TPA was produced from the hydrolyzation of PET in 228 h.Although PHB could not be synthesized directly from PET because of the low hydrolyzing efficiency of PETase,this study provides a new strategy for the biodegradation and upcycling of PET waste by artificial microflora. | Pan Liu Tong Zhang Yi Zheng Qingbin Li Tianyuan Su Qingsheng Qi | 2021 | Engineering Microbiology2021，1，1： | 3 |
| 2 | Biochemical characterization of a polyethylene terephthalate hydrolase and design of high-throughput screening for its directed evolution显示文摘Polyethylene terephthalate(PET),one of the most widely used plastics in the world,causes serious environmental pollution.Recently,researchers have focused their efforts on enzymatic degradation of PET,which is an attractive way of degrading and recycling PET.In this work,PET hydrolase Sb PETase from Schlegelella brevitalea sp.nov.was biochemically characterized,and rational design was performed based on its sequence similarity with the previ-ously reported Is PETase from Ideonella sakaiensis,resulting in a triple mutant with increased activity.Furthermore,using a sec-dependent signal peptide PeIB and colicin release protein Kil,we set up a high-efficiency secretion system of PETase in Escherichia coli BL21(DE3),enabling higher PETase secretion.Utilizing this secretion system,we established a high-throughput screening method named SecHTS(sec retion-based h igh-throughput s creening)and performed directed evolution of Is PETase and Sb PETase through DNA shuffling.Finally,we generated a mutant Is PETase S139T with increased activity from the mutant library. | Xiaotong Wang Chaoyi Song Qingsheng Qi Youming Zhang Ruijuan Li Liujie Huo | 2022 | Engineering Microbiology2022，2，2： | 2 |
| 3 | Dihydroxy acetone :Production and uses显示文摘 | GREEN S R WHALEN E A MOLOKIE E | 1961 | Journal of Biochemical and Microbiological Technology and Engineering1961，3，4： | 1 |
| 4 | Fermentation process kinetics 显示文摘 | GADEN E L | 1959 | Journal of Biochemical and Microbiological Technology and Engineering1959，4，1： | 1 |
| 5 | Fermentation process kinetics显示文摘 | GoAen E L | 2004 | Journal of Biochemical and Microbiological Technology and Engineering2004，1，4： | 1 |
| 6 | Dihydroxyacetone:production and uses显示文摘 | Green S R Whalen E A Molokie E | 1961 | Journal of Biochemical and Microbiological Technology and Engineering1961，3，4： | 1 |
| 7 | Control of stock culture preservation and inoculum build-up in bacterial fermentation显示文摘 | Lincoln R E | 1960 | Journal of Biochemical and Microbiological Technology and Engineering1960，2，4： | 1 |
| 8 | Fermentation process kinetics 显示文摘 | Gaden E L | 1959 | Journal of Biochemical and Microbiological Technology and Engineering1959，1，4： | 1 |
| 9 | A kinetic study of the lactic acid fermentation batch process at controlled pH 显示文摘 | Luedeking R Piret EL | 1959 | Journal of Biochemical and Microbiological Technology and Engineering1959，1，4： | 1 |
| 10 | Fermentation kinetics and productivity显示文摘 | Gaden E L | 1959 | Journal of Biochemical and Microbiological Technology and Engineering1959，1，4： | 1 |
| 11 | A kinetic study of the lactic acid fermentation batch process at controlled pH显示文摘 | Luedeking R Piret E L | 1959 | Journal of Biochemical and Microbiological Technology and Engineering1959，1，4： | 1 |
| 12 | Metabolic engineering using acetate as a promising building block for the production of bio‐based chemicals显示文摘The production of biofuels and biochemicals derived from microbial fermentation has received a lot of attention and interest in light of concerns about the depletion of fossil fuel resources and climatic degeneration.However,the economic viability of feedstocks for biological conversion remains a barrier,urging researchers to develop renewable and sustainable low-cost carbon sources for future bioindustries.Owing to the numerous advantages,acetate has been regarded as a promising feedstock targeting the production of acetyl-CoA-derived chemicals.This review aims to highlight the potential of acetate as a building block in industrial biotechnology for the production of bio-based chemicals with metabolic engineering.Different alternative approaches and routes com-prised of lignocellulosic biomass,waste streams,and C1 gas for acetate generation are briefly described and evaluated.Then,a thorough explanation of the metabolic pathway for biotechnological acetate conversion,cel-lular transport,and toxin tolerance is described.Particularly,current developments in metabolic engineering of the manufacture of biochemicals from acetate are summarized in detail,with various microbial cell factories and strategies proposed to improve acetate assimilation and enhance product formation.Challenges and future development for acetate generation and assimilation as well as chemicals production from acetate is eventually shown.This review provides an overview of the current status of acetate utilization and proves the great potential of acetate with metabolic engineering in industrial biotechnology. | Guiping Gong Bo Wu Linpei Liu Jianting Li Qili Zhu Mingxiong He Guoquan Hu | 2022 | Engineering Microbiology2022，2，4： | 1 |
| 13 | A kinetic study of the lactic acid fer- mentation显示文摘 | Leudeking R Piret E | 1959 | Journal of Biochemical and Microbiological Technology and Engineering1959，1，4： | 1 |
| 14 | Dihydroxyacetone: production and uses显示文摘 | Green S R Whalcn E A Molokie E | 1961 | Journal of Biochemical and Microbiological Technology and Engineering1961，4，3： | 1 |
| 15 | A kinelic study of the lactic acid fermentation, batch process at controlled pH 显示文摘 | LUEDEKINC R PIRET E L | 1959 | Journal of Biochemical and Microbiological Technology and Engineering1959，1，4： | 1 |
| 16 | A strategy to enhance the insecticidal potency of Vip3Aa by introducing additional cleavage sites to increase its proteolytic activation efficiency显示文摘Microbially derived,protein-based biopesticides have become a vital element in pest management strategies.Vip3 family proteins from Bacillus thuringiensis have distinct characteristics from known insecticidal Cry toxins and show efficient insecticidal activity against several detrimental lepidopteran pests.They are considered to be a promising toxic candidate for the management of various detrimental pests.In this study,we found that in addition to the preliminary digestion sites lysine,there are multiple cleavage activation sites in the linker region between domain I(DI)and DII of Vip3Aa.We further demonstrated that by adding more cleavage sites between DI and DII of Vip3Aa,its proteolysis efficiency by midgut proteases can be significantly increased,and correspondingly enhance its insecticidal activity against Spodoptera frugiperda and Helicoverpa armigera larvae.Our study promotes the understanding of the insecticidal mechanism of Vip3 proteins and illustrates an eas-ily implementable strategy to increase the insecticidal potency of Vip3Aa.This facilitates their potential future development and efficient application for sustainable agriculture. | Kun Jiang Zhe Chen Yiting Shi Yuanrong Zang Chengbin Shang Xi Huang Jiahe Zang Zhudong Bai Xuyao Jiao Jun Cai Xiang Gao | 2023 | Engineering Microbiology2023，3，4： | 1 |
| 17 | Fermentation process kinetics显示文摘 | Elmer L Gaden J R | 1959 | Journal of Biochemical and Microbiological Technology and Engineering1959，7，4： | 1 |
| 18 | Peptaibols:Diversity, bioactivity, and biosynthesis显示文摘Peptaibols are a large family of linear,amphipathic polypeptides consisting of 5-20 amino acid residues gener-ated from the fungal nonribosomal peptide synthetase(NRPS)pathway.With a relatively high content of non-proteinogenic amino acids such as��-aminoisobutyrate(Aib)and isovaline(Iva)in the skeleton,peptaibols exhibit a wide range of biological activities,including anti-microbial,cytotoxic,and neuroleptic effects.With five peptai-bols brought to market for use as biocontrol agents,this class of peptides has received increasing attention from both biochemists and pharmacologists.In this review,we summarized the progress made in structural character-ization,elucidation of biosynthetic pathways,and investigation of biosynthesis elucidation and bioactivities,to promote further efforts to develop peptaibols as pharmaceuticals. | Xuewen Hou Ruonan Sun Yanyan Feng Runfang Zhang Tianjiao Zhu Qian Che Guojian Zhang Dehai Li | 2022 | Engineering Microbiology2022，2，3： | 1 |
| 19 | Isolation, biosynthesis, and biological activity of rubromycins derived from actinomycetes显示文摘Natural occurring aromatic polyketides from actinomycetes indicate a structurally and functionally diverse fam-ily of polycyclic polyphenols.Some of them are consequently suggested as lead structures for drug development.Among them,rubromycins are derived from a single C26 polyketide chain and exhibit an unusual bisbenzannu-lated[5,6]-spiroketal system that connects a highly oxygenated naphthazarin motif to an isocoumarin unit.This type of biosynthetically elusive polycyclic polyketides has shown promising pharmacological activities,includ-ing antimicrobial,anticancer,and enzyme inhibition activity.The unique structures,intriguing biosynthesis,and marked bioactivities of rubromycins have drawn considerable attention from several chemists and biologists.This review covers the isolation,characterization,biosynthesis,and biological studies of these structurally diverse and complex rubromycins. | Ping Lin Xue Li Yuchen Xin Hongying Li Gang Li Hongxiang Lou | 2022 | Engineering Microbiology2022，2，3： | 1 |
| 20 | Fermentation process kinetics显示文摘 | Gaden E L | 1959 | Journal Biochemical and Microbiological Technology and Engineering1959，1，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员