Generation of tooth-like structures from integration-free human urine induced pluripotent stem cells

查看全文 作　　者：Jinglei [1]Cai;Yanmei [1]Zhang;Pengfei [1,2]Liu;Shubin [1]Chen;Xuan [1,2]Wu;Yuhua [3]Sun;Ang [4]Li;Ke [1]Huang;Rongping [1]Luo;Lihui [1]Wang;Ying [1,5]Liu;Ting [1]Zhou;Shicheng [3,6]Wei;Guangjin [1]Pan;Duanqing [1]Pei

机构地区：[1]CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cell Biology and Regenerative Medicine,South China Institute for Stem Cell Biology and Regenerative Medicine,Guangzhou Institute of Biomedicine and Health,Chinese Academy of Sciences,190 Kai Yuan Avenue,Science Park,Guangzhou 510530,P.R.China;[2]Department of Regeneration Medicine,School of Pharmaceutical,Jilin University,Changchun,P.R.China;[3]Department of Oral and Maxillofacial Surgery,Laboratory of Interdisciplinary Studies,School and Hospital of Stomatology,Peking University,Beijing,P.R.China;[4]Biodynamic Optical Imaging Center(BIOPIC),Peking University,Beijing,P.R.China;[5]The Shenzhen Key Lab of Gene and Antibody Therapy,Center for Biotech&Biomedicine and Division of Life Sciences,Graduate School at Shenzhen,Tsinghua University,Shenzhen,P.R.China;[6]Center for Biomedical Materials and Tissue Engineering,Academy for Advanced Interdisciplinary Studies,Peking University,Beijing,P.R.China.

出　　处：《Cell Regeneration》索引2013年第2卷第1期,共8页

基　　金：We thank Prof.Yanding Zhang and Prof.Dajiang Qin for valuable suggestions and all staffs working for the South Stem Cell Bank.This work was supported by the grants from Ministry of Science and Technology 973 Program(2010CB944800,2011CB965200);National Natural Science Foundation of China(31000402);the“Strategic Priority Research Program”of the Chinese Academy of Sciences(XDA01020401,XDA01020202);863 Program(2011AA020109);Ministry of Science and Technology International Technology Cooperation Program(2012DFH30050);Open Project of Key Laboratory of Regenerative Biology,Chinese Academy of Sciences(KLRB201217). 摘　　要：Background:Tooth is vital not only for a good smile,but also good health.Yet,we lose tooth regularly due to accidents or diseases.An ideal solution to this problem is to regenerate tooth with patients’own cells.Here we describe the generation of tooth-like structures from integration-free human urine induced pluripotent stem cells(ifhU-iPSCs).Results:We first differentiated ifhU-iPSCs to epithelial sheets,which were then recombined with E14.5 mouse dental mesenchymes.Tooth-like structures were recovered from these recombinants in 3 weeks with success rate up to 30%for 8 different iPSC lines,comparable to H1 hESC.We further detected that ifhU-iPSC derived epithelial sheets differentiated into enamel-secreting ameloblasts in the tooth-like structures,possessing physical properties such as elastic modulus and hardness found in the regular human tooth.Conclusion:Our results demonstrate that ifhU-iPSCs can be used to regenerate patient specific dental tissues or even tooth for further drug screening or regenerative therapies. 关键词：Human urine Integration-free iPSCs Recombinant tooth Bioengineered tooth Dental epithelium

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Generation of tooth-like structures from integration-free human urine induced pluripotent stem cells

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