Systematically labeling developmental stage-specific genes for the study of pancreatic β-cell differentiation from human embryonic stem cells

查看全文 作　　者：Haisong [1]Liu;Huan [1]Yang;Dicong [1]Zhu;Xin [1]Sui;Juan [2]Li;Zhen [1]Liang;Lei [3]Xu;Zeyu [4]Chen;Anzhi [4]Yao;Long [5]Zhang;Xi [5]Zhang;Xing [4]Yi;Meng [1]Liu;Shiqing [6]Xu;Wenjian [6]Zhang;Hua [7]Lin;Lan [8]Xie;Jinning [6]LOU;Yong [5]Zhang;Jianzhong [2]Xi;Hongkui [1,4,9]Deng

机构地区：[1]Shenzhen Stem Cell Engineering Laboratory, Key Laboratory of Chemical Genomics, Peking University Shenzhen Graduate School Shenzhen, Guangdong 518055, China;[2]Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China;[3]Department of Hematopoietic Stem Cell Transplantation, 307 Hospital, Academy of Military Medicine Sciences, Beijing 100071, China;[4]The MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China;[5]BGI-Shenzhen, Shenzhen, Guangdong 518083, China;[6]Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China;[7]Department of Gynecology and Obstetrics, China-Japan Friendship Hospital, Beijing 100029, China;[8]Department of Gynecology and Obstetrics, Beijing Renhe Hospital Beijing 102600, China;[9]Peking University Stem Cell Research Center, Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China

出　　处：《Cell Research》索引2014年第24卷第10期,共20页

基　　金：We thank Chenyan Wang, Yang Zhao, Yan Shi and Jun Xu for critical reading of the manuscript and for discussion in the preparation of this manuscript. We also thank Xiaobao Wang for con- ducting molecular cloning; Weichao Du, Bingqing Xie and Zijian Li for cell culture; Yinan Liu for the RT-qPCR analysis; Li Su, Shiliang Ma, Liying Du and National Center for Protein Sciences at Peking University (including but not limited to Zhonglin Fu; Zailing Bai and Jingshu Wang) for FACS; Hongxia Lv and Xiao-chen Li for confocal microscopy; and Junhua Zou for G-banding analysis. This work was supported by the National Basic Research Program of China (973 program; 2012CB966401), the Key New Drug Creation and Manufacturing Program (2011ZX09102-010- 03), the Ministry of Science and Technology (2013DFG30680), the National Natural Science Foundation of China (30830061), the Ministry of Education of China (111 project), and National Science and Technology Major Projects Supporting Program from Shenzhen (GJHS20120820102148947). 关键词：人胚胎干细胞特异性基因细胞分化发育阶段系统胰腺细胞表达动力学内分泌细胞

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Systematically labeling developmental stage-specific genes for the study of pancreatic β-cell differentiation from human embryonic stem cells

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