Degradation of dichloromethane by an isolated strain Pandoraea pnomenusa and its performance in a biotrickling filter

查看全文 作　　者：Jianming [1]Yu;Wenji [2]Cai;Zhuowei [2]Cheng;Jianmeng [2]Chen

机构地区：[1]Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology;[2]College of Biological and Environmental Engineering, Zhejiang University of Technology

出　　处：《Journal of Environmental Sciences》索引2014年第26卷第5期,共10页

基　　金：supported by the Major Science and Technology Project of Zhejiang Province(No.2011C13023);the Ph.D. Programs Foundation of Ministry of Education of China(No.20093317110003) 摘　　要：A strain Pandoraea pnomenusa LX-1 that uses dichloromethane(DCM) as sole carbon and energy source has been isolated and identified in our laboratory. The optimum aerobic biodegradation of DCM in batch culture was evaluated by response surface methodology. Maximum biodegradation(5.35 mg/(L·hr)) was achieved under cultivation at 32.8°C, pH 7.3, and 0.66% NaCl. The growth and biodegradation processes were well fitted by Haldane's kinetic model, yielding maximum specific growth and degradation rates of 0.133 hr-1and 0.856 hr-1, respectively. The microorganism efficiently degraded a mixture of DCM and coexisting components(benzene, toluene and chlorobenzene). The carbon recovery(52.80%–94.59%) indicated that the targets were predominantly mineralized and incorporated into cell materials. Electron acceptors increased the DCM biodegradation rate in the following order: mixed > oxygen > iron > sulfate > nitrate. The highest dechlorination rate was 0.365 mg Cl-/(hr·mg biomass), obtained in the presence of mixed electron acceptors. Removal was achieved in a continuous biotrickling filter at 56%–85% efficiency, with a mineralization rate of 75.2%. Molecular biology techniques revealed the predominant strain as P. pnomenusa LX-1. These results clearly demonstrated the effectiveness of strain LX-1 in treating DCM-containing industrial effluents. As such, the strain is a strong candidate for remediation of DCM coexisting with other organic compounds. 关键词：生物滴滤塔二氯甲烷菌株分离好氧生物降解分子生物学技术生物降解率性能

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Degradation of dichloromethane by an isolated strain Pandoraea pnomenusa and its performance in a biotrickling filter

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