CAPE promotes the expansion of human umbilical cord blood-derived hematopoietic stem and progenitor cells in vitro

查看全文 作　　者：LIU [1]YiMing;ZHANG [1]BoWen;ZHANG [1]Jing;WANG [1]SiHan;YAO [1]HaiLei;HE [1]LiJuan;CHEN [1]Lin;YUE [1]Wen;LI [1]YanHua;PEI [1]XueTao

机构地区：[1]Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine

出　　处：《Science China(Life Sciences)》索引2014年第57卷第2期,共7页

基　　金：supported by the National High Technology Research and Development Program of China(2013AA020107);National Basic Research Program of China(2011CB964804);National Natural Science Foundation of China(31101040) 摘　　要：Due to the low number of collectable stem cells from single umbilical cord blood(UCB)unit,their initial uses were limited to pediatric therapies.Clinical applications of UCB hematopoietic stem and progenitor cells(HSPCs)would become feasible if there were a culture method that can effectively expand HSPCs while maintaining their self-renewal capacity.In recent years,numerous attempts have been made to expand human UCB HSPCs in vitro.In this study,we report that caffeic acid phenethyl ester(CAPE),a small molecule from honeybee extract,can promote in vitro expansion of HSPCs.Treatment with CAPE increased the percentage of HSPCs in cultured mononuclear cells.Importantly,culture of CD34+HSPCs with CAPE resulted in a significant increase in total colony-forming units and high proliferative potential colony-forming units.Burst-forming unit-erythroid was the mostly affected colony type,which increased more than 3.7-fold in 1μg mL 1CAPE treatment group when compared to the controls.CAPE appears to induce HSPC expansion by upregulating the expression of SCF and HIF1-α.Our data suggest that CAPE may become a potent medium supplement for in vitro HSPC expansion. 关键词：CAPE 造血干细胞体外扩增祖细胞脐带血人类自我更新能力咖啡酸苯乙酯

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CAPE promotes the expansion of human umbilical cord blood-derived hematopoietic stem and progenitor cells in vitro

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