Time-resolved fluoroimmunoassay for quantitative determination of tylosin and tilmicosin in edible animal tissues

查看全文 作　　者：WEI [1]Shu;LE [1,2]Tao;CHEN [2]Yong;XU [3]Jian;HE [2]HongQiu;NIU [2]XiaoDong;LUO [2]JinHua

机构地区：[1]College of Life Science,Chongqing Normal University;[2]Chongqing Academy of Science and Technology;[3]Chongqing RuoFeng Bio-Tech Co.,Ltd

出　　处：《Chinese Science Bulletin》索引2013年第58卷第15期,共5页

基　　金：supported by the Scientific and Technological Research Project of Chongqing (CSTC2011ggB10009,cstc2012pt-kyys10002,cstc2012cx-rkxB10002);the National Natural Science Foundation of China(81202438);the Natural Science Foundation of the Chongqing Education Committee (KJ110605) 摘　　要：To quantitatively determine tylosin and tilmicosin in edible animal tissues,a time-resolved fluoroimmunoassay(TRFIA) has been developed and validated.For this purpose,desmycosin-O-carboxymethoxylamine-BSA was fixed onto microtiter plates,standards and samples were loaded and,finally,diluted europium-labeled anti-tylosin antibodies were added.Results show that the limit of detection for tylosin was 0.03 ng mL-1 and that for tilmicosin was 0.05 ng mL-1.The recoveries were 73.6% to 120.5%,with coefficients of variation below 15.6% in various biological matrices spiked with tylosin and tilmicosin at concentrations of 50-200 ngg-1.There was good correlation(R2>0.99) between the TRFIA,an enzyme-linked immunosorbent assay and high performance liquid chromatography data.In conclusion,the new TRFIA is applicable to the detection of tylosin and tilmicosin and is an effective and economical method that will enable high-throughput sample screening.The method is expected to be widely applicable. 关键词：时间分辨荧光泰乐菌素替米考星动物组织定量测定荧光免疫食用生物样品

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Time-resolved fluoroimmunoassay for quantitative determination of tylosin and tilmicosin in edible animal tissues

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