miR-200 family expression is downregulated upon neoplastic progression of Barrett's esophagus

查看全文 作　　者：Cameron M [1]Smith;David I [1]Watson;Mary P [1]Leong;George C [1]Mayne;Michael Z [2]Michael;Bas PL [3]Wijnhoven;Damian J [1]Hussey

机构地区：[1]Department of Surgery, Flinders University, Bedford Park;[2]Department of Gastroenterology and Hepatology, Flinders University, Bedford Park;[3]Department of Surgery, Erasmus Medical Centre

出　　处：《World Journal of Gastroenterology》索引2011年第17卷第8期,共9页

基　　金：Supported by National Health and Medical Research Council, Australia 摘　　要：AIM: To investigate miR-200 family expression in Barrett's epithelium, gastric and duodenal epithelia, and esophageal adenocarcinoma. METHODS: Real-time reverse transcriptase-polymerase chain reaction was used to measure miR-200, ZEB1 and ZEB2 expression. Ingenuity Pathway Analysis of miR-200 targets was used to predict biological outcomes. RESULTS: Barrett's epithelium expressed lower levels of miR-141 and miR-200c than did gastric and duodenal epithelia (P < 0.001). In silico analysis indicated roles for the miR-200 family in molecular pathways that distinguish Barrett's epithelium from gastric and duodenalepithelia, and which control apoptosis and proliferation. All miR-200 members were downregulated in adenocarcinoma (P < 0.02), and miR-200c expression was also downregulated in non-invasive epithelium adjacent to adenocarcinoma (P < 0.02). The expression of all miR-200 members was lower in Barrett's epithelium derived high-grade dysplastic cell lines than in a cell line derived from benign Barrett's epithelium. We observed signif icant inverse correlations between miR-200 family expression and ZEB1 and ZEB2 expression in Barrett's epithelium and esophageal adenocarcinoma (P < 0.05). CONCLUSION: miR-200 expression might contribute to the anti-apoptotic and proliferative phenotype of Barrett's epithelium and regulate key neoplastic processes in this epithelium. 关键词：食管肿瘤 miR 家族肠上皮细胞聚合酶链反应路径分析细胞表达细胞凋亡

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miR-200 family expression is downregulated upon neoplastic progression of Barrett's esophagus

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