Engineering Deinococcus radiodurans into biosensor to monitor radioactivity and genotoxicity in environment

查看全文 作　　者：GAO GuanJun FAN Lu LU HuiMing HUA [1]YueJin

机构地区：[1]Institute of Nuclear-Agricultural Sciences, Key Laboratory of Chinese Ministry of Agriculture for Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, China

出　　处：《Chinese Science Bulletin》索引2008年第53卷第11期,共7页

基　　金：the National Basic Research Program (Grant No. 2004CB19604);National Hi-Tech Development Program (Grant No. 2007AA021305);Distinguished Young Scientist (Grant No. 30425038);key project from the National Natural Science Foundation of China (Grant No. 30330020) 摘　　要：Based on a genetically modified radioresistant bacteria Deinococcus radiodurans, we constructed a real time whole cell biosensor to monitor radioactivity and genotoxicity in highly radioactive environ-ment. The enhanced green fluorescence protein (eGFP) was fused to the promoter of the crucial DNA damage-inducible recA gene from D. radiodurans, and the consequent DNA fragment (PrecA-egfp) car-ried by plasmid was introduced into D. radiodurans R1 strain to obtain the biosensor strain DRG300. This engineered strain can express eGFP protein and generate fluorescence in induction of the recA gene promoter. Based on the correlation between fluorescence intensity and protein expression level in live D. radiodurans cells, we discovered that the fluorescence induction of strain DRG300 responds in a remarkable dose-dependent manner when treated with DNA damage sources such as gamma radiation and mitomycin C. It is encouraging to find the widely detective range and high sensitivity of this re-constructed strain comparing with other whole cell biosensors in former reports. These results suggest that the strain DRG300 is a potential whole cell biosensor to construct a detective system to monitor the biological hazards of radioactive and toxic pollutants in environment in real time. 关键词：生物传感器环境监测放射能环境污染

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Engineering Deinococcus radiodurans into biosensor to monitor radioactivity and genotoxicity in environment

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