In vitro biofilm formation by uropathogenic Escherichia coli and their antimicrobial susceptibility pattern

查看全文 作　　者：Poovendran [1]Ponnusamy;Vidhya [1]Natarajan;Murugan [2]Sevanan

机构地区：[1]Department of Microbiology,Dr.N.G.P Arts and Science College,Coimbatore-641048,Tamil Nadu,India;[2]Department of Biotechnology,School of Biotechnology and Health Sciences,Karunya University,Coimbatore-641114,Tamil Nadu,India

出　　处：《Asian Pacific Journal of Tropical Medicine》索引2012年第5卷第3期,共4页

摘　　要：Objective:To detect in vitro biofilm formation of uropathogenic Escherichia coli(E.coli)(UPEC) strains isolated from urine specimens and also to determine their antimicrobial susceptibility pattern using 13 commonly used antibiotics.Methods:The present study comprised of 166 urine specimens collected from tertiary care hospitals in and around Coimbatore.South India. All the specimens were subjected to gram staining,bacterial culture and the E.coli strains were screened for biofilm formation using Tube Method(TM),Congo Red Agar(CRA) and Tissue Culture Plate method(TCP) respectively.Subsequently,the antimicrobial susceptibility test was performed by Kirby Bauer-disk diffusion method for the biofilm and non-biofilm producing E. coli strains.Results:Of the 100(60.2%) E.coli strains,72 strains displayed a biofilm positive phenotype under the optimized conditions in the Tube Method and the strains were classified as highly positive(17,23.6%),moderate positive(19.26.3%) and weakly positive(36.50.0%). similarly under the optimized conditions on Congo Red agar medium,biofilm positive phenotype strains were classified as highly positive(23,23%).moderate positive(37.37%) and weakly positive(40,40%).While in TCP method,the biofilm positive phenotype strains were also classified as highly positive(6.6%),moderate positive(80.80%) and weakly positive(14,14%),it didn’t not correlate well with the tube method for detecting biofilm formation in E.coli.The rates of antibiotic resistance of biofilm producing E.coli were found to be 100%for chloramphenicol and amoxyclav(amoxicillin and clavulanic acid),86%for gentamicin and cefotaxime.84%for ceftazidime,83%for cotrimoxazole and piperacillin/tazobactam,75%for tetracycline and 70% for amikacin,Conclusions:This study reveals the prevalence and antimicrobial susceptibility pattern of biofilm and non-biofilm producing uropathogenic E.coli strains. 关键词：URINARY TRACT infection ESCHERICHIA COLI BIOFILM Multi-drug resistance

相关文献

参考文献（35）
引证文献（5）
耦合文献（7）

参考文献(35)

引证文献(5)

耦合文献(7)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

In vitro biofilm formation by uropathogenic Escherichia coli and their antimicrobial susceptibility pattern

参考文献(35)

引证文献(5)

耦合文献(7)

用户提示