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Construction of a cucumber genetic linkage map with SRAP markers and location of the genes for lateral branch traits

查看全文 作　　者：WANG Gang PAN Junsong LI Xiaozun HE Huanle WU Aizhong CAI [1]Run 高影响力作者 机构地区：[1]Agriculture and Biology School of Shanghai Jiaotong University, Shanghai,201101, China Agriculture and Biology School of Shanghai Jiaotong University, Shanghai,201101, China Agriculture and Biology School of Shanghai Jiaotong University, Shanghai,201101, China Agriculture and Biology School of Shanghai Jiaotong University, Shanghai,201101, China Agriculture and Biology School of Shanghai Jiaotong University, Shanghai,201101, China Agriculture and Biology School of Shanghai Jiaotong University, Shanghai,201101, China高影响力机构 出　　处：《Science China(Life Sciences)》索引2005年第48卷第3期,共8页高影响力期刊 基　　金：This work was support ed by the Shanghai Municipal Scientifi c&Technological Commission(Grant Nos.033107019);Shanghai Municipal Agricultural Commission(Grant Nos 2003-2-1-2). 摘　　要：Using SRAP (sequence-related amplified polymorphism) markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F2 individuals derived from a cross of the two cucumber lines, S06 and S52. In the survey of parental polymorphisms with 182 primer combinations, 64 polymorphism-revealing primer pairs were screened out, which generated totally 108 polymorphic bands with an average of 1.7 bands per primer pair and at most 6 bands from one primer pair. The constructed molecular linkage map included 92 loci, distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of 12.6 cM between two neighboring loci. Based on this linkage map, the quantitative trait loci (QTL) for the lateral branch number (lbn) and the lateral branch average length (lbl) in cucumber were identified by QTLMapper1.6. A major QTL lbn1 located between ME11SA4B and ME5EM5 in LG2 could explain 10.63% of the total variation with its positively effecting allele from S06. A major QTL lbl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38% of the total variation with its positively effecting allele from S06. 关 键 词：cucumber, SRAP (sequence-related amplified polymorphism), molecular LINKAGE map, lateral branch gene, quantitative TRAIT LOCI (QTL).
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