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| 1 | 干眼症眼表损害炎症机制显示文摘干眼症为一种多因素造成泪膜稳定性和眼表功能损害的疾病,易引起眼部不适、视力障碍、泪膜不稳定与眼表的潜在危害,可伴有泪液渗透压升高及眼表炎症反应。炎症是干眼发病中最关键因素,多种免疫细胞和炎症因子参与了干眼症的发生与发展过程。细胞凋亡、神经调节异常、性激素失调等也共同参与了干眼的发病过程。最近尽管在阐述干眼的病理生理和发病机制取得了一定进展,但目前还未形成统一标准。本文将对炎症造成干眼症眼表和泪液功能损害的可能机制进行综述。 | 宿梦苍 郝晓琳 张仲臣 | 2015 | 国际眼科杂志2015，15，5： | 97 |
| 2 | Immune suppression in chronic hepatitis B infection associated liver disease: A review显示文摘Hepatitis B virus(HBV) infection is one the leading risk factors for chronic hepatitis, liver fibrosis, cirrhosis and hepatocellular cancer(HCC), which are a major global health problem. A large number of clinical studies have shown that chronic HBV persistent infection causes the dysfunction of innate and adaptive immune response involving monocytes/macrophages, dendritic cells, natural killer(NK) cells, T cells. Among these immune cells, cell subsets with suppressive features have been recognized such as myeloid derived suppressive cells(MDSC),NK-reg, T-reg, which represent a critical regulatory system during liver fibrogenesis or tumourigenesis. However, the mechanisms that link HBVinduced immune dysfunction and HBV-related liver diseases are not understood.In this review we summarize the recent studies on innate and adaptive immune cell dysfunction in chronic HBV infection, liver fibrosis, cirrhosis, and HCC, and further discuss the potential mechanism of HBV-induced immunosuppressive cascade in HBV infection and consequences. It is hoped that this article will help ongoing research about the pathogenesis of HBV-related hepatic fibrosis and HBV-related HCC. | Tian-Yang Li Yang Yang Guo Zhou Zheng-Kun Tu | 2019 | World Journal of Gastroenterology2019，25，27： | 69 |
| 3 | Molecular mechanisms of liver ischemia reperfusion injury:Insights from transgenic knockout models显示文摘Ischemia reperfusion injury is a major obstacle in liver resection and liver transplantation surgery.Understanding the mechanisms of liver ischemia reperfusion injury(IRI) and developing strategies to counteract this injury will therefore reduce acute complications in hepatic resection and transplantation,as well as expanding the potential pool of usable donor grafts.The initial liver injury is initiated by reactive oxygen species which cause direct cellular injury and also activate a cascade of molecular mediators leading to microvascular changes,increased apoptosis and acute inflammatory changes with increased hepatocyte necrosis.Some adaptive pathways are activated during reperfusion that reduce the reperfusion injury.IRI involves a complex interplay between neutrophils,natural killer T-cells cells,CD4+ T cell subtypes,cytokines,nitric oxide synthases,haem oxygenase-1,survival kinases such as the signal transducer and activator of transcription,Phosphatidylinositol 3-kinases/Akt and nuclear factor κβ pathways.Transgenic animals,particularly genetic knockout models,have become a powerful tool at elucidating mechanisms of liver ischaemia reperfusion injury and are complementary to pharmacological studies.Targeted disruption of the protein at the genetic level is more specific and maintained than pharmacological inhibitors or stimulants of the same protein.This article reviews the evidence from knockout models of liver IRI about the cellular and molecular mechanisms underlying liver IRI. | Gourab Datta Barry J Fuller Brian R Davidson | 2013 | World Journal of Gastroenterology2013，19，11： | 50 |
| 4 | 肝郁、脾虚和肝郁脾虚证模型大鼠的免疫功能变化显示文摘目的比较肝郁、脾虚和肝郁脾虚3种不同证候模型大鼠的免疫功能变化。方法雄性Wistar大鼠随机分为正常对照组、肝郁组、脾虚组、肝郁脾虚组4组,每组10只。其中肝郁组、脾虚组、肝郁脾虚组大鼠分别采用慢性束缚、过度疲劳+饮食失节、慢性束缚+过度疲劳+饮食失节的方法,连续造模4周。实验第29天,测定各组大鼠血清白介素-1(IL-1)、白介素-2(IL-2)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α),脾T、B淋巴细胞增殖率及胸腺、脾脏指数。结果与正常组相比,肝郁组、脾虚组和肝郁脾虚组大鼠血清IL-2、IL-6及胸腺、脾脏指数均显著降低(P<0.05);肝郁组血清IL-1显著降低(P<0.05)、TNF-α显著升高(P<0.05),脾虚组脾T淋巴细胞增殖率显著降低(P<0.05)、血清IL-1显著升高(P<0.05);肝郁脾虚组血清TNF-α和IL-1均显著升高(P<0.05),脾T淋巴细胞增殖率显著降低(P<0.05)。结论肝郁、脾虚、肝郁脾虚3个证候模型均存在一定程度的免疫功能异常,但同中有异:肝郁证主要涉及胸腺功能下降及免疫应答早期细胞因子释放不足,脾虚证还涉及细胞免疫功能抑制,肝郁脾虚证不仅包括了肝郁和脾虚2证的免疫异常环节,可能还伴有体液免疫功能抑制。 | 赵荣华 谢鸣 李聪 王旭丹 张敬升 张雪庭 | 2013 | 北京中医药大学学报2013，36，12： | 44 |
| 5 | Standard triple therapy for Helicobacter pylori infection in China: A meta-analysis显示文摘AIM:To assess the efficacy and safety of standard triple therapy compared with other pre-existing and new therapies in China.METHODS:Literature searches were conducted in the following databases:PubMed,EMBASE,the Cochrane Central Register of Controlled Trials,the VIP database,the China National Knowledge Infrastructure database,and the Chinese Biomedical Database.A meta-analysis of all randomized controlled trials(RCTs)comparing standard triple therapy for the eradication of Helicobacter pylori with pre-existing and new therapies in China was performed using Comprehensive Meta-Analysis 2.0.There were 49 studies that met our criteria and the qualities of these studies were assessed using the Jadad scale.The Mantel-Haenszel method was used for pooling dichotomous data.We also conducted subgroupanalyses according to age,duration of treatment and drug type.Sensitivity analyses and a cumulative metaanalysis were also performed with CMA 2.0.Publication bias was evaluated using Egger’s test,Begg’s test or a funnel plot.RESULTS:A total of 49 RCTs including 8332 patients were assessed.This meta-analysis showed that standard triple therapy with proton pump inhibitors(PPIs),amoxicillin(AMO)and clarithromycin(CLA)was inferior to sequential therapy[relative risk(RR)=0.863;95%confidence interval(CI):0.824-0.904],but was not superior to quadruple therapy(RR=1.073;95%CI:0.849-1.357)or other triple therapies(RR=1.01;95%CI:0.936-1.089).The meta-analysis also suggested that standard triple therapy is slightly more effective than dual therapy(RR=1.14;95%CI:0.99-1.31).However,the differences were not statistically significant.We removed the only trial with a regimen lasting14 d by sensitivity analysis and found that 7-d standard triple therapy was superior to 7-d dual therapy(RR=1.222;95%CI:1.021-1.461).Moreover,a sub-analysis based on the duration of quadruple therapy indicated that the 7-d and 10-d standard triple therapies were inferior to sequential therapy(RR=0.790;95%CI:0.718-0.868;RR=0.917;95%CI:0.839-1.002,respectively).Additionally,there were no significant differences in cure rate or adverse events among standard triple therapy,quadruple therapy,and other triple therapies(RR=0.940;95%CI:0.825-1.072;RR=1.081;95%CI:0.848-1.378,respectively).Standard triple therapy had a higher occurrence of side effects than sequential therapy(RR=1.283;95%CI:1.066-1.544).CONCLUSION:The eradication rates with a standard triple therapy consisting of PPI,AMO,and CLA are suboptimal in China,and new treatment agents need to be developed. | Ben Wang Zhi-Fa Lv You-Hua Wang Hui Wang Xiao-Qun Liu Yong Xie Xiao-Jiang Zhou | 2014 | World Journal of Gastroenterology2014，20，40： | 46 |
| 6 | Preoperative evaluation of colorectal cancer using CT colonography, MRI, and PET/CT显示文摘Imaging studies are a major component in the evaluation of patients for the screening,staging and surveillance of colorectal cancer.This review presents commonly encountered findings in the diagnosis and staging of patients with colorectal cancer using computed tomography(CT)colonography,magnetic resonance imaging(MRI),and positron emission tomography(PET)/CT colonography.CT colonography provides important information for the preoperative assessment of T staging.Wall deformities are associated with muscular or subserosal invasion.Lymph node metastases from colorectal cancer often present with calcifications.CT is superior to detect calcified metastases.Three-dimensional CT to image the vascular anatomy facilitates laparoscopic surgery.T staging of rectal cancer by MRI is an established modality because MRI can diagnose rectal wall laminar structure.N staging in patients with colorectal cancer is still challenging using any imaging modality.MRI is more accurate than CT for the evaluation of liver metastases.PET/CT colonography isvaluable in the evaluation of extra-colonic and hepatic disease.PET/CT colonography is useful for obstructing colorectal cancers that cannot be traversed colonoscopically.PET/CT colonography is able to localize synchronous colon cancers proximal to the obstruction precisely.However,there is no definite evidence to support the routine clinical use of PET/CT colonography. | Shigeyoshi Kijima Takahiro Sasaki Koichi Nagata Kenichi Utano Alan T Lefor Hideharu Sugimoto | 2014 | World Journal of Gastroenterology2014，20，45： | 46 |
| 7 | Restoring the Treg cell to Th17 cell ratio may alleviate HBV-related acute-on-chronic liver failure显示文摘AIM: To investigate the role of T helper 17 cells (Th17) and regulatory T cells (Treg) in hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF).METHODS: We enrolled 79 patients with HBV infection into the study, 50 patients with HBV-related ACLF and 29 patients with chronic hepatitis B (CHB), from the First Affiliated Hospital of Medical College from January 2009 to June 2012. The ACLF patients were diagnosed according to the criteria recommended by The 19th Conference of the Asian Pacific Association for the Study of the Liver in 2009. Twenty healthy individuals with a similar gender and age structures to the two patient groups were also included as the normal controls (NC). Of the 50 ACLF patients, 28 were subsequently classified as non-survivors: 19 patients died from multiorgan failure, 3 underwent liver transplantation, and 6 discontinued therapy during follow-up because of financial reasons. The remaining 22 ACLF patients whose liver and anticoagulation function recovered to nearly normal levels within the next 6 mo were classified as survivors. The number of circulating Treg and Th17 cells was determined upon diagnosis and during the 8th week of follow-up through flow cytometry. RESULTS: The percentage of circulating Treg cells in the ACLF group was significantly higher than that in the CHB group (5.50% ± 1.15% vs 3.30% ± 1.13%, P < 0.01). The percentages of circulating Th17 cells in the ACLF and the CHB groups were significantly higher than that in the NC group (6.32% ± 2.22% vs 1.56% ± 0.44%, P < 0.01; 3.53% ± 1.65% vs 1.56% ± 0.44%, P < 0.01). No significant difference in Treg cell to Th17 cell ratio was observed between the ACLF group and the CHB group (0.98 ± 0.44 vs 1.12 ± 0.64, P = 0.991), whereas those in the two HBV infection groups were significantly lower than that in the NC group (1.85 ± 1.22; both P < 0.01). The percentage of Treg cells in the survivors during the 8th week of follow-up was significantly lower than that during peak ACLF severity [total bilirubin (TBIL) peak] (3.45% ± 0.97% vs 5.18% ± 1.02%, P < 0.01). The percentage of Th17 cells in survivors during the 8th week of follow-up was significantly lower than that during the peak TBIL (2.89% ±0.60% vs 5.24% ± 1.46%; P < 0.01). The Treg cell to Th17 cell ratio during the 8 th week of follow-up was significantly higher than that during the TBIL peak (1.22 ± 0.36 vs 1.10 ± 0.54; P < 0.05). CONCLUSION: Restoring the Treg cell to Th17 cell ratio during the follow-up phase of ACLF could maintain the immune system at a steady state, which favours good prognosis. | Ying-Hua Niu Dong-Lin Yin Hong-Li Liu Rui-Tian Yi Yu-Cong Yang Hong-An Xue Tian-Yan Chen Shu-Lin Zhang Shu-Mei Lin Ying-Ren Zhao | 2013 | World Journal of Gastroenterology2013，19，26： | 36 |
| 8 | 槐杞黄颗粒佐治小儿咳嗽变异性哮喘对免疫球蛋白、T淋巴细胞亚群及细胞因子的影响显示文摘目的:分析槐杞黄颗粒佐治小儿咳嗽变异性哮喘(CVA)时,对患儿免疫球蛋白、T淋巴细胞亚群及细胞因子的影响。方法:选取于我院收治的小儿CVA患儿80例,随机分为研究组及对照组,两组均给予沙丁胺醇粉雾剂,对照组在此基础上给予孟鲁司特钠咀嚼片,而研究组给予槐杞黄颗粒,分析治疗3个月后患儿IL-4、IFN-γ、IgA、IgG及IgE水平及CD4^+、CD8^+T细胞比例。结果:治疗前两组各指标比较,差异均无统计学意义(P>0.05);治疗后两组IgA及IgG均显著升高,而IgE显著降低(P<0.05)。研究组IgA及IgG升高较对照组显著,IgE降低较对照组显著(P<0.05)。治疗后,与对照组相比,研究组CD4^+比例及CD4^+/CD8^+下降程度更为显著,CD8^+比例升高更显著(P<0.05)。治疗后IL-4水平显著下降,而IFN-γ及IFN-γ/IL-4显著增高(P<0.05),研究组IL-4水平下降较对照组明显,IFN-γ升高较对照组明显,治疗后IFN-γ/IL-4水平增高显著高于对照组(P<0.05)。结论:槐杞黄颗粒可有效改善CVA患儿体液免疫和细胞免疫,同时可调节Th1/Th2的细胞免疫平衡,有助于提高CVA患儿免疫功能,改善预后。 | 张红艳 赵淑景 田菲 | 2017 | 海南医学院学报2017，23，3： | 38 |
| 9 | 基于NB-IoT的农田远程监测系统的设计显示文摘为了实时了解农作物生长环境信息,综合运用传感器技术、嵌入式技术和基于蜂窝的窄带物联网(Narrow Band Internet of Things,NB-IoT)等先进的信息技术,设计了基于NB-IoT的农田远程监测系统。此系统可对农田空气温湿度、土壤温湿度、光照强度、二氧化碳浓度、土壤pH值进行监测,并利用NB-IoT网络将实时采集的农田环境数据上传到后台管理服务器。后台服务器部署的农田环境数据监测平台采用LNMP(Linux+Nginx+My SQL+PHP)网站服务器架构实现,用户可使用浏览器访问农田环境数据监测平台来获取农田环境数据。该系统具有功能实用、操作简单、可大规模部署等特点。 | 王能辉 胡国强 | 2017 | 陕西农业科学2017，63，12： | 35 |
| 10 | 外周中心静脉置管在小儿化疗中的应用显示文摘目的 探讨经外周静脉穿刺导入中心静脉导管(PICC)在小儿化疗中的应用效果。方法 对138例化疗患儿采用美国巴德公司生产的单腔导管,从上肢肘静脉穿刺置管于上腔静脉进行化疗。结果 经贵要静脉插管123例,肘正中静脉12例,头静脉5例,一次置管成功率为94.2%,平均置管时间125.6±37.2天,最长456天,置管期间无严重并发症及继发感染。结论 PICC是一种操作方便、安全、并发症少、保留时间长的深静脉置管术,能有效减低化疗药物对血管的毒性作用,但必须做好患儿的心理护理,注意穿刺血管的选择及插入导管的长度,术后护理及带管出院的护理。 | 梁海华 谢巧庆 张婷婷 郑志惠 黄敏英 | 2004 | 护士进修杂志2004，19，3： | 36 |
| 11 | 脓毒症患者T淋巴细胞亚群变化及与炎症状态的关系研究显示文摘目的研究脓毒症患者的T 淋巴细胞亚群变化及与炎症状态的关系。方法选取2015 年3 月—2018 年10 月浙江大学医学院附属杭州市第一人民医院脓毒症患者126 例作为观察组,另选取同期健康体检者60 例作为对照组。检测并比较两组的T 淋巴细胞亚群(CD4+、CD8+、CD4+/CD8+)和炎症因子[血沉(ESR)、降钙素原(PCT)、C 反应蛋白(CRP)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、肿瘤坏死因子α(TNF-α)、高迁移率族蛋白B1(HMGB-1)],采用Pearson 检验T 淋巴细胞亚群与炎症指标的相关性。结果观察组入院时CD8^+及血清PCT、CRP、IL-6、TNF-α、IL-10、HMGB-1 水平高于对照组(P <0.05),而CD4+、CD4+/CD8+低于对照组(P <0.05)。PCT、CRP、IL-6、IL-10、TNF-α在入院6 h 达到峰值;严重脓毒症组和脓毒症休克组各个时间点的CD4^+、CD4^+/CD8^+低于脓毒症组,CD8^+、PCT、CRP、IL-6、IL-10、TNF-α高于脓毒症组,ESR 在入院5 d 时高于脓毒症组,而HMGB-1 在入院7 d 时高于脓毒症组,差异有统计学意义(P <0.05),且严重脓毒症组与脓毒症休克组比较,差异有统计学意义(P <0.05)。脓毒症患者的CD4^+、CD8^+、CD4^+/CD8^+与血清PCT、CRP、IL-6、IL-10、TNF-α和HMGB-1 均呈线性相关(P <0.05)。结论脓毒症患者存在明显的T 淋巴细胞紊乱和促炎/抗炎失衡,且病情越重其免疫-炎症紊乱越明显,动态监测T 淋巴细胞亚群及炎症因子水平可为病情评估及治疗决策提供参考。 | 叶瑞 胡炜 刘炳炜 席绍松 朱英 未亚平 | 2019 | 中国现代医学杂志2019，29，17： | 32 |
| 12 | 补肾化瘀方联合克罗米芬治疗多囊卵巢综合症的疗效观察显示文摘目的:探究补肾化瘀方联合克罗米芬治疗多囊卵巢综合症的临床效果。方法:选取符合纳入标准的多囊卵巢患者72例,随机分为治疗组和对照组,每组36例,治疗组采用补肾化瘀方联合克罗米芬治疗,对照组采用克罗米芬治疗,共治疗3个月经周期。观察两组患者治疗前后的性激素水平、治疗过程中的排卵情况及不良反应发生情况。结果:治疗组的性激素LH、E2、T水平明显优于对照组,治疗组排卵率75.56%明显高于对照组的61.96%,治疗组总有效率为86.11%高于对照组的63.89%,治疗组不良反应率为5.56%低于对照组的22.22%,P均<0.05,差异均具有统计学意义。结论:补肾化瘀方联合克罗米芬治疗多囊卵巢综合征能有效的稳定性激素水平,促进排卵,提高治愈率,且不良反应小,是治疗PCOS的有效方法。 | 伍立群 李波 | 2017 | 中医药信息2017，34，2： | 29 |
| 13 | T-SPOT.TB、痰涂片和TB-DNA检测在肺结核诊断中的比较研究显示文摘目的评价T-SPOT.TB、痰涂片和TB-DNA 3种方法对肺结核病人的临床应用价值。方法应用T-SPOT.TB、痰涂片和TB-DNA 3种检测方法分别对确诊组130例肺结核(TB)患者及60例非结核疾病患者进行检测。结果 T-SPOT.TB、痰涂片和TB-DNA在肺结核检测中的敏感性分别为92.31%、26.15%、71.54%,在对照组中其特异性分别为93.33%、100%、97.87%。T-SPOT.TB在'涂阴'肺结核中的阳性率为94.79%,在TB-DNA检测阴性的肺结核中检出率为94.59%。结论 3种方法中,痰涂片敏感性最低,T-SPOT.TB最高,极大地减少了结核的漏检率,可以成为结核病早期诊断的一项有效的辅助检查手段。 | 张玉平 丁显平 张丽媛 李晓阳 丁敏 李凌霄 | 2013 | 成都医学院学报2013，8，1： | 28 |
| 14 | 宫颈癌术后感染患者T淋巴细胞免疫功能变化及其临床意义显示文摘目的:探讨宫颈癌患者手术前后及术后发生感染时 T 淋巴细胞免疫功能的变化,以指导术后临床药物的使用。方法选择2012年10月-2013年9月在妇科宫颈癌Ⅰ A ~Ⅱ A 期行广泛子宫切除+盆腔淋巴结清扫术患者50例,其中33例术后无感染患者为手术Ⅰ组,17例合并术后感染患者为手术Ⅱ组;另选取20名健康女性为对照Ⅰ组及20例子宫肌瘤行全子宫切除术患者为对照Ⅱ组,采用 ELISA 法检测各组血清中γ干扰素(IFN-γ)、白细胞介素2(IL-2)和肿瘤坏死因子α(TNF-α);采用流式细胞术检测外周血中 IFN-γ+ CD4+、IL-2+ CD4+和TNF-α+ CD4+等 T 淋巴细胞亚群。结果手术Ⅰ、Ⅱ组的宫颈癌患者 IFN-γ+ CD4+、IL-2+ CD4+和 TNF-α+CD4+ T 淋巴细胞及其分泌的 IFN-γ、IL-2和 TNF-α水平在术前显著低于对照Ⅰ、Ⅱ组(P<0.05);手术Ⅰ、Ⅱ组的以上各项免疫指标在术后显著升高(P<0.05),且术后合并感染的手术 Ⅱ组明显高于未合并感染的手术Ⅰ组(P<0.05)。结论宫颈癌患者体内 T 淋巴细胞分泌细胞因子功能受抑制;术后 T 淋巴细胞免疫功能有恢复趋势,而感染进一步增强免疫反应。 | 谭细凤 徐慧君 郭丽华 谷雨枫 邰立志 段跃 | 2015 | 中华医院感染学杂志2015，25，6： | 28 |
| 15 | Effects of early enteral nutrition on Th17/Treg cells and IL-23/IL-17 in septic patients显示文摘BACKGROUND The imbalance of Th17/Treg cells and the IL-23/IL-17 axis have been confirmed to be associated with sepsis and various inflammatory diseases. Early enteral nutrition (EEN) can modulate the inflammatory response, improve immune dysfunction, and prevent enterogenic infection in critically ill patients;however, the precise mechanisms remain unclear. Considering the important roles of Th17 and Treg lymphocytes in the development of inflammatory and infectious diseases, we hypothesized that EEN could improve the immune dysfunction in sepsis by maintaining a balanced Th17/Treg cell ratio and by regulating the IL- 23/IL-17 axis. AIM To investigate the effects of EEN on the Th17/Treg cell ratios and the IL-23/IL-17 axis in septic patients. METHODS In this prospective clinical trial, patients were randomly divided into an EEN or delayed enteral nutrition (DEN) group. Enteral feeding was started within 48 h in the EEN group, whereas enteral feeding was started on the 4th day in the DEN group. The Th17 and Treg cell percentages and the interleukin levels were tested on days 1, 3, and 7 after admission. The clinical severity and outcome variables were also recorded. RESULTS Fifty-three patients were enrolled in this trial from October 2017 to June 2018. The Th17 cell percentages, Th17/Treg cell ratios, IL-17, IL-23, and IL-6 levels of the EEN group were lower than those of the DEN group on the 7th day after admission (P < 0.05). The duration of mechanical ventilation and of the intensive care unit stay of the EEN group were shorter than those of the DEN group (P <0.05). However, no difference in the 28-d mortality was found between the two groups (P = 0.728). CONCLUSION EEN could regulate the imbalance of Th17/Treg cell ratios and suppress the IL- 23/IL-17 axis during sepsis. Moreover, EEN could reduce the clinical severity of sepsis but did not reduce the 28-d mortality of septic patients. | Jia-Kui Sun Wen-Hao Zhang Wen-Xiu Chen Xiang Wang Xin-Wei Mu | 2019 | World Journal of Gastroenterology2019，25，22： | 27 |
| 16 | Role of epithelial-mesenchymal transition in gastric cancer initiation and progression显示文摘Gastric cancer is one of the most common malignant tumors worldwide.Due to its intricate initiation and progression mechanisms,early detection and effective treatment of gastric cancer are difficult to achieve.The epithelial-mesenchymal transition(EMT)is characterized as a fundamental process that is critical for embryonic development,wound healing and fibrotic disease.Recent evidence has established that aberrant EMT activation in the human stomach is closely associated with gastric carcinogenesis and tumor progression.EMT activation endows gastric epithelial cells with increased characteristics of mesenchymal cells and reduces their epithelial features.Moreover,mesenchymal cells tend to dedifferentiate and acquire stem cell or tumorigenic phenotypes such as invasion,metastasis and apoptosis resistance as well as drug resistance during EMT progression.There are a number of molecules that indicate the stage of EMT(e.g.,E-cadherin,an epithelial cell biomarker);therefore,certain transcriptional proteins,especially E-cadherin transcriptional repressors,may participate in the regulation of EMT.In addition,EMT regulation may be associated with certain epigenetic mechanisms.The aforementioned molecules can be used as early diagnostic markers for gastric cancer,and EMT regulation can provide potential targets for gastric cancer therapy.Here,we review the role of these aspects of EMT in gastric cancer initiation and development. | Zhao Peng Chen-Xiao Wang Er-Hu Fang Guo-Bin Wang Qiang Tong | 2014 | World Journal of Gastroenterology2014，20，18： | 28 |
| 17 | Astragalus polysaccharide attenuates rat experimental colitis by inducing regulatory T cells in intestinal Peyer's patches显示文摘AIM: To explore probable mechanism underlying the therapeutic effect of Astragalus polysaccharide(APS) against experimental colitis.METHODS: Thirty-two Sprague-Dawley rats were randomly divided into four groups. Colitis was induced with 2, 4, 6-trinitrobenzene sulfonic acid(TNBS). The rats with colitis were treated with 400 mg/kg of APS for 7 d. The therapeutic effect was evaluated by colonic weight, weight index of the colon, colonic length, and macroscopic and histological scores. The levels of regulatory T(Treg) cells in Peyer's patches were measured by flow cytometry, and cytokines in colonic tissue homogenates were analyzed using enzyme-linked immunosorbent assay. The expression of related orphan receptor-gt(ROR-gt), IL-23 and STAT-5a was measured by Western blot.RESULTS: After 7-d treatment with APS, the weight index of the colon, colonic weight, macroscopical and histological scores were decreased, while the colonic length was increased compared with the model group. The expression of interleukin(IL)-2, IL-6, IL-17, IL-23 and ROR-gt in the colonic tissues was down-regulated, but Treg cells in Peyer's patches, TGF-β and STAT5 a in the colonic tissues were up-regulated.CONCLUSION: APS effectively ameliorates TNBSinduced experimental colitis in rats, probably through restoring the number of Treg cells, and inhibiting IL-17 levels in Peyer's patches. | Hai-mei Zhao Yan Wang Xiao-Ying Huang min-fang Huang Rong Xu Hai-Yang Yue Bu-gao Zhou Hong-Yan Huang Qi-meng Sun Duan-Yong Liu | 2016 | World Journal of Gastroenterology2016，22，11： | 28 |
| 18 | 结核诊断金标准探讨显示文摘 | 周为鸿 | 2006 | 实用诊断与治疗杂志2006，20，10： | 26 |
| 19 | 肺炎支原体感染后机体的体液免疫、外周血T淋巴细胞亚群含量的评估显示文摘目的:评估肺炎支原体感染后机体的体液免疫、外周血T淋巴细胞亚群含量。方法:118例感染肺炎支原体的患儿作为观察组,同期在本院接受体检的健康儿童98例作为对照组,对比两组体液免疫、T淋巴细胞亚群、抗炎及促炎因子、Th1/Th2平衡状态等差异。结果:观察组血清IgA水平低于对照组(P<0.05),IgM水平高于对照组(P<0.05);外周血CD3^+、CD4^+T淋巴细胞水平及CD4^+/CD8^+比值低于对照组(P<0.05),CD8^+T淋巴细胞水平高于对照组(P<0.05);血清促炎因子白介素(IL)-8、肿瘤坏死因子-α(TNF-α)水平高于对照组(P<0.05),抗炎因子IL-10、IL-13水平低于对照组(P<0.05);血清IL-4、γ-干扰素(IFN-γ)、IL-4/IFN-γ水平高于对照组(P<0.05)。结论:肺炎支原体感染后患儿体液免疫及细胞免疫功能抑制,高炎症状态等均是导致疾病进展的重要机制。 | 黎素清 | 2017 | 海南医学院学报2017，23，3： | 25 |
| 20 | 尤瑞克林联合依达拉奉对急性脑梗死患者作用机制的研究显示文摘目的:通过对急性脑梗死患者联合应用尤瑞克林和依达拉奉进行治疗,研究其对患者血清氧化低密度脂蛋白(ox-LDL)、降钙素原(PCT)、超敏C-反应蛋白(hs-CRP)、肿瘤坏死因子-α(TNF-α)和T细胞亚群的影响,探讨联合用药对急性脑梗死患者的作用机制。方法:选取86例在我院进行诊治的急性脑梗死患者,随机分为对照组和观察组,每组43例患者。所有患者均根据自身具体情况给予一般治疗方案,包括降糖、调压、防治并发症、对症支持治疗等。对照组在此基础上给予30mg依达拉奉注射液静脉滴注,每天1次,连用14天;观察组在对照组治疗的基础上加用0.15PNA单位尤瑞克林静脉滴注,每天1次,连用14天。于治疗前后检测并比较两组血清ox-LDL、PCT、hs-CRP、TNF-α以及CD3^+、CD4^+、CD8^+、CD4^+/CD8^+水平的变化。结果:(1)治疗前,两组血清ox-LDL、PCT、hs-CRP、TNF-α水平之间比较,差异均无显著性(P>0.05);治疗后,两组血清ox-LDL、PCT、hs-CRP、TNF-α水平均明显降低,与同组治疗前相比,差异均具有显著性(P<0.05),且观察组血清ox-LDL、PCT、hsCRP、TNF-α水平改善程度明显优于对照组,差异均具有显著性(P<0.05)。(2)治疗前,两组血清CD3^+、CD4^+、CD8^+、CD4^+/CD8^+水平之间比较,差异均无显著性(P>0.05);治疗后,两组血清CD3^+、CD4^+、CD4^+/CD8^+水平明显升高,CD8^+水平明显降低,与同组治疗前相比,差异均具有显著性(P<0.05),且观察组血清CD3^+、CD4^+、CD8^+、CD4^+/CD8^+水平改善程度明显优于对照组,差异均具有显著性(P<0.05)。结论:对急性脑梗死患者联合应用尤瑞克林和依达拉奉进行治疗,可以明显改善患者血清ox-LDL、PCT、hs-CRP、TNF-a和T细胞亚群水平,进一步说明了其联合用药的协同增效作用,也说明两药用于急性脑梗死可以抑制血栓扩大,降低机体炎症反应,减轻脑组织损伤,改善神经功能。 | 杜春艳 王庆广 | 2017 | 海南医学院学报2017，23，3： | 25 |
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