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| 1 | Extracts of Celastrus Orbiculatus Inhibit Cancer Metastasis by Down-regulating Epithelial-Mesenchymal Transition in Hypoxia-Induced Human Hepatocellular Carcinoma Cells显示文摘Objective: To evaluate the effects of Celastrus Orbiculatus extracts(COE) on metastasis in hypoxiainduced hepatocellular carcinoma cells(Hep G2) and to explore the underlying molecular mechanisms. Methods:The effect of COE(160, 200 and 240 μg/mL) on cell viability, scratch-wound, invasion and migration were studied by 3-4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2-H-tetrazolium bromide(MTT), scratch-wound and transwell assays, respectively. Co Cl2 was used to establish a hypoxia model in vitro. Effects of COE on the expressions of E-cadherin, vimentin and N-cadherin were investigated with Western blot and immuno?uorescence analysis,respectively. Results: COE inhibited proliferation and metastasis of hypoxia-induced hepatocellular carcinoma cells in a dose-dependent manner(P<0.01). Furthermore, the expression of epithelial-mesenchymal transition(EMT) related markers were also remarkably suppressed in a dose-dependent manner(P<0.01). In addition, the upstream signaling pathways, including the hypoxia-inducible factor 1α(Hif-1α) and Twist1 were suppressed by COE. Additionally, the Hif-1α inhibitor 3-5'-hydroxymethyl-2'-furyl)-1-benzylindazole(YC-1), potently suppressed cell invasion and migration as well as expression of EMT in hypoxia-induced Hep G2 cells. Similarly, the combined treatment with COE and YC-1 showed a synergistic effect(P<0.01) compared with the treatment with COE or YC-1 alone in hypoxia-induced Hep G2 cells. Conclusions: COE signi?cantly inhibited the tumor metastasis and EMT by suppressing Hif-1α/Twist1 signaling pathway in hypoxia-induced Hep G2 cell. Thus, COE might have potential effect to inhibit the progression of Hep G2 in the context of tumor hypoxia. | QIAN Ya-yun SHI You-yang LU Song-hua YANG Ting ZHAO Xue-yu YAN Yan LI Wen-yuan LIU Yan-qing | 2019 | Chinese Journal of Integrative Medicine2019，25，5： | 12 |
| 2 | Celastrus orbiculatus Extracts Inhibit Human Hepatocellular Carcinoma Growth by Targeting mTOR Signaling Pathways显示文摘Objective:To characterize the molecular mechanism underlying the antineoplastic activity of Celastrus orbiculatus Thunb.extracts(COE).Methods:The human hepatocellular carcinoma HepG2 cells with mammalian target of rapamycin(mTOR)knockdown expressed(HepG2/mTOR-)were constructed using molecular biological technology.In vitro,the HepG2/mTOR-cells were treated with COE at various concentrations(10,20,40,80,160 and 320μg/mL).Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assays.According to the half-maximal inhibitory concentration(IC50)value(140 mg/L),the concentrations of COE in the subsequent experiment was set to alleviate cytotoxicity.The HepG2/mTOR-cells were divided into 5 groups:negative control(untreated),COE treatment groups(40,80,120 mg/L COE)and positive control group(cisplatin,DDP,2 mg/L),respectively.Wild-type HepG2 cells were used as a blank control.The effects of COE on the cell apoptosis were analyzed by flow cytometry and transmission electronic microscopy(TEM),respectively.The protein expression levels of mTOR signal pathways were determined by Western blotting.In vivo,HepG2/mTOR-cells(2×106 cell/mice)were subcutaneously injected into the right flank of nude mice.Thirty-six female nude mice were randomly assigned to 6 groups according to body weight(6 mice per group)as follows:solvent vehicle control,Banmao Capsule treated group(BM,195 mg/kg),Tegafur,Gimeracil and Oteracil Potassium Capsules(10 mg/kg)treated group,and different dosages of COE(10,20,40 mg/kg)groups.Tumor growth was monitored and immunohistochemical staining was used to examine the expression of apoptosis-related proteins in tumor tissues.Results:COE inhibited the proliferation significantly in a concentration-dependent manner in HepG2/mTOR-cells(P<0.01).COE significantly induced the apoptosis of HepG2/mTOR-cells(P<0.01),and the apoptotic bodies can be observed under TEM.COE significantly inhibits the proteins expression of mTORrelated signal pathways.In vivo,COE significantly inhibited tumor growth in nude mice(P<0.01).Moreover,the results showed that COE down-regulated the expression of Bcl-2 and Bcl-xL,and up-regulated the levels of Bax and caspase-3 protein(P<0.01).Conclusion:COE was a potential chemotherapeutic drug in HCC treatments via targeting mTOR signal pathway. | QIAN Ya-yun LI Wen-yuan YAN Yan ZHAO Xue-yu YANG Ting FANG Chuan-ci HOU Jing-jing LIU Yan-qing | 2019 | Chinese Journal of Integrative Medicine2019，25，11： | 4 |
| 3 | A Novel Sesquiterpene Polyol Ester from Celastrus Angulatus and its Antitumor Activities显示文摘A new sesquiterpene polyol ester was isolated from the leaves of Celastrus angulatus. itsstructure was determined as 1 6. 2 6. 9 a-triacetoxy-8 a-(a-hydroxyl-isobutyryloxy)-15-benzoyloxy-4 a-hydroxy-dihydroagarofuran by means of NMR. MS and IR spectral analysis.Preliminary biological study on antitumor activity revealed that this compound showed strongnonselective cytotoxicity against four of the NCI panel cell lines. | Wei Ping YIN Tian Zeng ZHAO Ling Jie GAO Da Peng ZOU Jian Xun KANG(Institute of Chemistry,Henan Academy of Sciences Zhengzhou 450003 Centre of Instrumental Analysis. Zhengzhou University. Zhengzhou 450052) | 1999 | Chinese Chemical Letters1999，10，6： | 2 |
| 4 | THE STRUCTURE OF CELAGLAUMIN——ANTITUMOR PRINCIPLE OF Celastrus glaucophyllus显示文摘Since pronounced antitumor activity displayed by maytansine and triptolide from the plants of Celastraceae was discovered, scientists investigated and screened the plants of Celastraceae phytochemically and pharmacologically, hoping to discover new antitumor compounds. Four sesquiterpene esters were isolated from the acetone extract of the root barks | 刘吉开 贾忠建 吴大刚 周俊 朱子清 | 1989 | Chinese Science Bulletin1989，34，19： | 2 |
| 5 | A New Sesquiterpene from Celastrus Angulatus显示文摘One new sesquiterpene polyol ester named angulatin C was isolated from the root barkof Celastrus angulalus along with a known compound, angulatin B.Their structures wereelucidated on the basis of spectral analysis. | Hai Lin QIN Tian Zeng ZHAO Yu Jun SHANG(Institute of Chemistry, Henan Academy of Sciences Zhengzhou 450002) | 1999 | Chinese Chemical Letters1999，10，10： | 2 |
| 6 | Animal-mediated long-distance dispersals and migrations shaping the intercontinental disjunctions of Celastrus(Celastraceae)among five continents显示文摘The mechanisms underlying the origin,evolution,and distributional patterns of organisms are a major focus of biogeography.Vicariance and long-distance dispersal(LDD)are two important explanations for disjunctive distribution patterns among lineages.In-depth biogeographic studies of taxa that exhibit wide-ranging disjunctions can provide valuable information for addressing the relative importance of these biogeographic mechanisms.The genus Celastrus contains ca.30 species that are disjunctly distributed in five continents of both the Northern and Southern Hemispheres,providing an excellent system for historical biogeographic analyses.Here,we used sequence data from five markers(nuclear external transcribed spacer and internal transcribed spacer,and plastid psbA-trnH,rpl16,and trnL-F)to reconstruct the phylogeny of Celastrus and investigate its phylogenetic relationships with Tripterygium,estimate clade divergence times using the fossil-calibrated method,and infer its ancestral distribution range.Celastrus and Tripterygium were each supported as monophyletic.The morphology-based classification systems were not supported by the phylogenetic results.The divergence time between Celastrus and Tripterygium was estimated to be 26.22 Ma(95%highest posterior density:24.46-28.17 Ma),and the diversification of Celastrus were suggested to be linked to global warming events during the Miocene.Celastrus was suggested to have a tropical Asian origin,and dispersed to Central and South America,North America,Oceania,and Madagascar at different periods,most probably through LDD.Birds may have facilitated transoceanic migrations of Celastrus because of its bicolored fruits,which contain red and fleshy arils.Our results highlight the importance of key morphological innovations and animal-mediated dispersals for the rapid diversification of plant lineages across vast distributional ranges. | Yi-Xuan Zhu Feng-Wei Lei Ling Tong Xian-Yun Mu Jun Wen Zhi-Xiang Zhang | 2020 | Journal of Systematics and Evolution2020，58，6： | 1 |
| 7 | A NEW SESQUITERPENE FROM CELASTRUS ROSTHONIANUS显示文摘A new sesquitcrpene was isolated from Celastrus rothornianus, its structure was clucidated mainly on the basis of spectral analysis. | Yong Qiang TU Department of Chemistry, Lanzhou University, Lanzhou, 730000 | 1991 | Chinese Chemical Letters1991，2，12： | 1 |
| 8 | INVESTIGATION OF CHEMICAL CONSTITUENT FROM CELASTRUS ANGULATUS显示文摘Three new β-dihydroagarofuran sesquiterpene polyol esters,one alkaloid andtwo non-alkaloids,were isolated from the MeOH extract of seed oil of Celastrus angulatus.Their structures were elucidated on the basis of UV,IR,mass,NOE difference,1H-NMR,13C-NMR and 1H-13C long-range correlation spectroscopy. | Yong Qiang TU Department of Chemistry,Lanzhou University,Lanzhou,730000 | 1992 | Chinese Chemical Letters1992，3，8： | 0 |
| 9 | CHEMICAL CONSTITUENTS AND BIOLOGICAL ACTIVITY OF CELASTRACEAE PLANTS显示文摘Ⅰ. INTRODUCTIONDihydroagarofuran sesquiterpenoids are the main chemical constituents of Celastraceae plants. Since the Celastraceae plants were used as agricultural insecticides, we are quite interested in the investigation on the insecticidal activity of this kind of compound. Our previous note reported that three sesquiterpenoids (1—3) were isolated | 涂永强 吴大刚 | 1992 | Chinese Science Bulletin1992，37，14： | 0 |
| 10 | FOUR NEW ALKALOIDS FROM Celastrus angulatus显示文摘The roots of Celastrus angulatus Maxim. are used in folk medicine for antipycetic and anti-wandering arthritis purpose. It also has been used as insecticide. The seven alkaloids were isolated from the root-bark of this plant. They are | 刘吉开 贾忠建 吴大刚 周俊 朱子清 | 1989 | Chinese Science Bulletin1989，34，12： | 0 |
| 11 | AN IHSECTICIDAL SESQUITERPENE FROM CELASTRUS ANGULATUS显示文摘An insecticidal sesquiterpene polyol ester,angulatin A,was isolated from the root barkof Celastrus angulatus.Its structure was established as 1α,2α-diacetoxy-8β,15-diisobutyryloxy-9α-benzoyloxy-4β,6β-dihydroxydihyclro-β-agarofuran by spectroscopic methods. | Mao Tian Wang Hai Lin Qin Yan Zi Li Henan Institute of Chemistry,Zhengzhou,450003Man Kong Institute of Materia Medica.Chinese Academy of Medical Seiences,Beijing,100050 | 1991 | Chinese Chemical Letters1991，2，7： | 0 |
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