| 1 | Multiomics comparison among populations of three plant sources of Amomi Fructus显示文摘Amomi Fructus(Sharen,AF)is a traditional Chinese medicine(TCM)from three source species(or varieties),including Wurfbainia villosa var.villosa(WVV),W.villosa var.xanthioides(WVX),or W.longiligularis(WL).Among them,WVV has been transplanted from its top-geoherb region,Guangdong,to its current main production area,Yunnan,for>50 years in China.However,the genetic and transcriptomic differentiation among multiple AF source species(or varieties)and between the origin and transplanted populations of WVV is unknown.In our study,the observed overall higher expression of terpenoid biosynthesis genes in WVV than in WVX provided possible evidence for the better pharmacological effect of WVV.We also screened six candidate borneol dehydrogenases(BDHs)that potentially catalyzed borneol into camphor in WVV and functionally verified them.Highly expressed genes at the P2 stage of WVV,Wv05G1424 and Wv05G1438,were capable of catalyzing the formation of camphor from(+)-borneol,(-)-borneol and DL-isoborneol.Moreover,the BDH genes may experience independent evolution after acquiring the ancestral copies,and the following tandem duplications might account for the abundant camphor content in WVV.Furthermore,four populations of WVV,WVX,and WL are genetically differentiated,and the gene flow from WVX to WVV in Yunnan contributed to the greater genetic diversity in the introduced population(WVV-JH)than in its top-geoherb region(WVV-YC),which showed the lowest genetic diversity and might undergo genetic degradation.In addition,terpene synthesis(TPS)and BDH genes were selected among populations of multiple AF source species(or varieties)and between the top-and non-top-geoherb regions,which might explain the difference in metabolites between these populations.Our findings provide important guidance for the conservation,genetic improvement,and industrial development of the three source species(or varieties)and for identifying top-geoherbalism with molecular markers,and proper clinical application of AF. | Xinlian Chen Shichao Sun Xiaoxu Han Cheng Li Fengjiao Wang Bao Nie Zhuangwei Hou Song Yang Jiaojiao Ji Ge Li Yanqian Wang Xiaoyu Han Jianjun Yue Cui Li Wei Li Lixia Zhang Depo Yang Li Wang | 2023 | Horticulture Research2023，10，8： | 0 |
| 2 | 千里香杂合度PCR-RFLP快速评估方法的建立显示文摘目的:建立一种快速评估千里香种质材料杂合度的方法,为制定千里香的优异种质繁育策略和促进种质创新提供依据。方法:以65株千里香重测序数据为基础,检测并筛选单核苷酸多态性(SNPs),利用其中20个SNP位点,通过自编脚本将其转化为限制性内切酶片段长度多态性(RFLP)标记;采用聚合酶链式反应-限制性内切酶长度多态性(PCR-RFLP)法对12份千里香种质的20个RFLP标记进行检测,根据RFLP标记位点酶切片段数目计算千里香种质的杂合度;采用plink软件计算12份千里香种质的全基因组杂合度,并比较不同种质杂合度评估方法得到的结果。结果:PCR-RFLP法和基因组重测序法计算的杂合度间差异无统计学意义。利用PCR-RFLP法筛选获得了8份杂合度<30%的种质材料,利用基因组重测序法筛选获得9份杂合度<30%的种质材料,2种方法计算杂合度均<30%的种质材料共7份。结论:该文建立了千里香种质杂合度评估的PCR-RFLP法,其精确度为87.5%,准确率为77.8%,该法可为其他药用植物种质杂合度评估方法研究提供借鉴。 | 王博铖 陈梓媛 华中一 田慧 谢文波 袁媛 | 2024 | 中国实验方剂学杂志2024，30，4： | 0 |
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