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| 1 | 植物胼胝质合成酶研究进展显示文摘胼胝质是一种β-1,3–葡萄糖聚合物,植物在生长发育和受到生物、非生物胁迫过程中均会有胼胝质沉积。胼胝质合成酶又称β-1,3–葡聚糖合成酶类似物,通常以多亚基复合物形式控制胼胝质的合成。本文中对胼胝质合成酶的分离鉴定过程进行了梳理,对胼胝质合成酶复合物的亚基及其作用进行了描述,重点总结了转录因子、植物生长调节剂等对胼胝质合成酶基因表达的调控作用,并对胼胝质合成酶在花粉发育和韧皮部运输过程中的作用,对机械损伤、磷酸盐、金属离子等非生物胁迫,以及虫害、细菌和真菌等生物胁迫的应答反应进行了归纳;最后对胼胝质合成酶的研究方向提出展望,以期为解析胼胝质合成酶的调控机制提供参考,也为植物(特别是园艺植物)抗性基因的挖掘提供借鉴。 | 张庆雯 王兆昊 祁静静 谢宇 雷天刚 何永睿 陈善春 姚利晓 | 2021 | 园艺学报2021，48，4： | 8 |
| 2 | Genome-wide Analysis of MYB Gene Family in Potato Provides Insights into Tissue-specific Regulation of Anthocyanin Biosynthesis显示文摘The MYB transcription factor is one of the largest gene families in plants,playing an important role in regulating plant growth,development,response to stress,senescence,and especially the anthocyanin biosynthesis.In this study,A total of 217 MYB genes,including 901R-MYBs,124 R2R3-MYBs,and 3 R1R2R3-MYBs have been identified from the potato genome.The 1R-MYB and R2R3-MYB family members could be divided into 20 and 35 subgroups respectively.Analysis of gene structure and protein motifs revealed that members within the same subgroup presented similar exon/intron and motif organization,further supporting the results of phylogenetic analysis.Potato is an ideal plant to reveal the tissue-specific anthocyanins biosynthesis regulated by MYB,as the anthocyanins could be accumulated in different tissues,showing colorful phenotypes.Five pairs of colored and colorless tissues,stigma,petal,stem,leaf,and tuber flesh,were applied to the transcriptomic analysis.A total of 70 MYB genes were found to be differentially expressed between colored and colorless tissues,and these differentially expressed genes were suspected to regulate the biosynthesis of anthocyanin of different tissues.Co-expression analysis identified numerous potential interactive regulators of anthocyanins biosynthesis,involving 39 MYBs,24 bHLHs,2 WD-repeats,and 29 biosynthesis genes.Genome-wide association study(GWAS)of tuber flesh color revealed amajor signal at the end of Chromosome 10,which was co-localized with reported I gene(StMYB88),controlling tuber peel color.Analyses of DEGs(Differentially Expression Genes)revealed that both StMYB88 and StMYB89 were closely related to regulating anthocyanin biosynthesis of tuber flesh.This work offers a comprehensive overview of the MYB family in potato and will lay a foundation for the functional validation of these genes in the tissue-specific regulation of anthocyanin biosynthesis. | Yumei Li Jun Liang Xiangzhi Zeng Han Guo Youwei Luo Philip Kear Shouming Zhang Guangtao Zhu | 2021 | Horticultural Plant Journal2021，7，2： | 8 |
| 3 | 南京地区盆栽百合引种适应性研究显示文摘[目的]本文旨在对引进的盆栽百合品种的生长适应性以及观赏特性进行综合评价与比较,筛选出适宜南京地区推广的盆栽百合品种和育种材料。[方法]采用百分制记分法对18个盆栽百合新品种(盆栽东方百合9个,盆栽亚洲百合9个)的物候期、植物学特性和综合抗性进行综合评价与比较,筛选适宜推广种植的百合品种和潜在的优良育种亲本。[结果]春季在连栋薄膜大棚内种植的13个盆栽百合品种中,8个盆栽亚洲百合能够正常生长发育,从定植到开花时间为58~74 d;植株挺直健壮,植株高度为25~45 cm;花色丰富艳丽,花形完整,单株有7~11朵花,多为单瓣;整体花期长15~25 d,无病虫害发生,适应性好、观赏价值高。盆栽东方百合观赏价值也很高,但多数品种蚜虫危害严重。秋季种植的13个盆栽百合品种经过低温处理后,盆栽亚洲百合品种依旧表现出较高的观赏价值和较强的适应性,从定植到开花时间为29~51 d,明显短于春季,花期长20~28 d,明显长于春季。盆栽东方百合品种除‘红马丁’‘瑞丽’和‘粉罗宾’外,其他盆栽东方百合生长异常,病虫害严重。[结论]盆栽亚洲百合观赏性和抗性均优于盆栽东方百合。南京及附近地区春、秋两季均适宜引种盆栽亚洲百合,而盆栽东方百合仅个别品种适宜在南京地区引种栽培,且需及时防治病虫害。 | 陈子琳 吴泽 张德花 国圆 滕年军 | 2021 | 南京农业大学学报2021，44，1： | 5 |
| 4 | 切花百合耐热性评价及越夏栽培技术研究显示文摘[目的]本研究利用所建立的切花百合耐热性评价体系,对不同品种进行打分,旨在筛选出较耐热型品种,并探究夏季栽培百合时低温生根处理技术对百合性状的影响。[方法]对5个品系共26个切花百合品种分别进行低温生根至有芽点出现后种植和低温生根至有新生根出现后种植2种处理,进行物候期观测统计和耐热性调查,依照切花百合耐热性评价体系标准打分。[结果]在供试的5个品系的切花百合中,平均分最高的是铁东百合杂种系(LO)系列百合,其次是东喇百合杂种系(OT)系列百合,最低的为东方百合。在低温生根处理对比试验中,有25个品种经过处理后得分增加。低温生根处理对亚洲百合和铁亚百合杂种系(LA)系列百合生育期缩短的效果较明显,对因高温所产生的叶烧和花蕾败育现象有改善作用,但改善程度因品种而异,而对植株倒伏现象没有明显改善。[结论]在夏季高温情况下可选择种植LO系列百合和OT系列百合;亚洲百合和LA系列百合虽生育期较短,但品质无法保证,应慎重选择;东方百合在高温条件下大多表现欠佳,不建议在夏季种植。在夏季可以采用低温生根处理的方式改良切花百合的观赏性状。 | 蓝令 吴泽 张德花 滕年军 | 2021 | 南京农业大学学报2021，44，6： | 4 |
| 5 | Study on the Physiological,Cellular,and Morphological Aspects of the Postharvest Development of Cut Lily Flowers显示文摘It is of great practical significance to study the development process of cut lily for regulating flowering and preservation.In this study,the developmental process of lily cut flower was explored from cellular,morphological,and physiological aspects.Froma morphological aspect,the tepal edge grows faster than the midrib.The midrib groove of the flowering stage is wider than that of bud stage.The fast-growing edge of the petals results in the midrib bending outward.Moreover,the rapid growth of the stamens and stigmas also contributes to bud cracking.From the cellular aspect,in the bud stage there were more wrinkles in the outer epidermal cell wall of the tepal than those in the inner epidermal cell wall,indicating that asymmetric structural differences exist from the beginning of lily development.From a physiological aspect,from the bud cracking stage to the senescence stage,a variety of substances in the tepal cells gradually decreased,including total nitrogen,total phosphorus,total potassium,total calcium,starch,soluble sugar,and soluble protein,but not malondialdehyde.These results indicate that the asymmetric growth caused by this structural difference is responsible for flowering.The wrinkles in the cell wall can be regarded as indicators of senescent cells and are caused by the degradation of the cell wall and the loss of intracellular turgor pressure.The differences in the epidermal cells between the inner and outer tepal indicated ultrastructural changes in the tepal cells.The bud and flowering stages are maintained by the tepals acting as a sink.After flowering,the tepals gradually change from a sink to a source organ.Senescence of the cut lily flowers was caused by the decomposition of intracellular compounds in the tepals and the remobilization of nutrients from the tepals to the developing organs. | Yushan Zhang Dexin Zhong Zhanfei Liu Jiawei Gao | 2021 | Horticultural Plant Journal2021，7，2： | 2 |
| 6 | LIWRKY39 is involved in thermotolerance by activating LIMBF1c and interacting with LICaM3 in lily(Lilium longiflorum)显示文摘WRKY transcription factors(TFs)are of great importance in plant responses to different abiotic stresses.However,research on their roles in the regulation of thermotolerance remains limited.Here,we investigated the function of LlWRKY39 in the thermotolerance of lily(Lilium longiflorum‘white heaven’).According to multiple alignment analyses,LIWRKY39 is in the WRKY IId subclass and contains a potential calmodulin(CaM)-binding domain.Further analysis has shown that LlCaM3 interacts with LlWRKY39 by binding to its CaM-binding domain,and this interaction depends on Ca^(2).LIWRKY39 was induced by heat stress(HS),and the LIWRKY39-GFP fusion protein was detected in the nucleus.The thermotolerance of lily and Arabidopsis was increased with the ectopic overexpression of LlWRKY39.The expression of heat-related genes AtHSFA1,AtHSFA2,AtMBF1c,AtGolS1,AtDREB2A,AtWRKY39,and AtHSP101 was significantly elevated in transgenic Arabidopsis lines,which might have promoted an increase in thermotolerance.Then,the promoter of LlMBF1c was isolated from lily,and LlWRKY39 was found to bind to the conserved W-box element in its promoter to activate its activity,suggesting that LlWRKY39 is an upstream regulator of LlMBF1c.In addition,a dual-luciferase reporter assay showed that via protein interaction,LlCaM3 negatively affected LlWRKY39 in the transcriptional activation of LlMBF1c,which might be an important feedback regulation pathway to balance the LlWRKY39-mediated heat stress response(HSR).Collectively,these results imply that LlWRKY39 might participate in the HSR as an important regulator through Ca^(2+)-CaM and multiprotein bridging factor pathways. | Liping Ding Ze Wu Renda Teng Sujuan Xu Xing Cao Guozhen Yuan Dehua Zhang Nianjun Teng | 2021 | Horticulture Research2021，8，1： | 1 |
| 7 | 芍药属植物远缘杂交亲和性及其雌蕊的生理响应机制显示文摘为探究不同芍药属植物远缘杂交的亲和性,筛选出亲和性较好的杂交组合,以芍药老来红(LLH)、蝶恋花(DLH)、鲁红(LH)、莲台(LT)、星光灿烂(XGCL)、遍地红(BDH)、Kansas、Venus和Peter Brand 9个品种为母本,以牡丹凤丹白(FDB)为父本进行远缘杂交。测定授粉后不同时间(1 h、2 h、4 h、8 h、12 h、24 h)雌蕊中的保护酶[过氧化物酶(POD)、超氧化物歧化酶(SOD)]活性、胼胝质(β-1,3-葡聚糖)含量及β-1,3-葡聚糖酶活性,并对授粉后花粉粒萌发及花粉管生长情况进行荧光显微观察。结果表明,授粉后2 h观察到花粉粒开始萌发,BDH×FDB、Kansas×FDB和Peter Brand×FDB 3个组合的花粉粒萌发相对较晚。随后在花粉管的生长过程中,胼胝质不断沉积,花粉管开始出现扭曲、缠绕等现象。授粉后24 h,LLH×FDB、LH×FDB和Kansas×FDB 3个组合可以观察到花粉管进入柱头,但未进入子房。POD、SOD活性在9个组合间及同一组合授粉后不同时间表现出显著差异,当POD、SOD活性较高时,花粉粒萌发较快且花粉管正常生长。胼胝质含量及β-1,3-葡聚糖酶活性在不同组合间差异显著,高含量的胼胝质及低活性的β-1,3-葡聚糖酶会导致花粉管出现扭曲缠绕、肿胀等异常现象。国内的芍药品种中,LLH×FDB、LH×FDB组合的亲和性较好,BDH×FDB组合的亲和性最差;国外的芍药品种中,Kansas×FDB组合的亲和性相对较好,Peter Brand×FDB组合的亲和性最差。 | 贺丹 曹健康 何松林 符真珠 华超 张明星 王政 | 2023 | 江苏农业学报2023，39，3： | 0 |
| 8 | GAMYB transcription factor LoMYB65 from lily plays a vital role in pollen development显示文摘Lily(Lilium spp.)is an important horticultural crop,but its use is limited due to serious pollen contamination problems.There are many studies on pollen development in model plants,but few on flower crops such as lilies.Gibberellin(GA)is a large class of hormones and plays an important role in plant vegetative growth and reproductive development.GAMYB is a group of the R2R3-MYB family upregulated by gibberellin,and plays an important role in anther development.Here,we isolated a novel GAMYB,named LoMYB65,from lily,which was closely related to the AtMYB65 and AtMYB33 in Arabidopsis.Fluorescence quantitative PCR results showed that LoMYB65 was mainly expressed in lily anthers.LoMYB65 could be activated by 288μmol·L^(-1)GA3treatment and the LoMYB65 protein was located in the nucleus and cytoplasm,and had transactivation in yeast and tobacco leaf cells.The conserved motif within 226 amino acids of the C-terminal of LoMYB65 contributed to its transactivation.Overexpression of LoMYB65 caused dwarf phenotype,unnormal tapetum development,less seeds of siliques in transgenic Arabidopsis plants,the transgenic plants showed partly male sterile.Simultaneously,silencing of LoMYB65 with VIGS(Virus Induced Gene Silencing)in lily anthers caused unnormal pollen development and reduced the pollen amount.Overexpression of LoMYB65 in Arabidopsis and silencing of LoMYB65 in lily resulted in decreased pollen counts,so we speculate that LoMYB65 may be dose-dependent.Overall,these findings suggest that LoMYB65 may play an important role in anther development and pollen formation in lily.LoMYB65 may provide a useful candidate gene for pollenless breeding of lily. | Xinyue Liu Ling He Ze Wu Nianjun Teng | 2024 | Horticultural Plant Journal2024，10，1： | 0 |
| 9 | R2R3-type Lo MYB21 affects jasmonate-regulated development and dehiscence of anthers in lily(Lilium oriental hybrids)显示文摘Lilies are widely cultivated for cut flowers,but their large anthers carry a considerable amount of colored pollen that is dispersed easily.Studying the molecular mechanism of anther development and dehiscence could help solve this problem.LoMYB21,encoding a putative R2R3v-myb avian myeloblastosis viral oncogene homolog(MYB)transcription factor,was identified from oriental lilies(Lilium‘Siberia’).Real-time quantitative PCR analysis showed that LoMYB21 was mainly expressed in the anther,filament and stigma and had high expression during the late stages of lily anther development.LoMYB21 had transactivation activity and was located in the nucleus through yeast one-hybrid assays and transient expression in Nicotiana benthamiana.Suppression of LoMYB21 by virus-induced gene silencing(VIGS)in Lilium‘Siberia’led to anther indehiscence and reduced the expression of genes related to Jasmonate acid(JA)biosynthesis and signal transduction.Induction of LoMYB21 in DEX::LoMYB21 transgenic Arabidopsis caused procumbent inflorescences that became infertile,accompanied by higher expression of JA biosynthetic and signaling genes.These results demonstrated that JA content and signaling were abnormal in silenced lily and transgenic LoMYB21 Arabidopsis,which affected anther development.Our study indicated that LoMYB21 could regulate lily anther dehiscence through JA biosynthesis and signaling during the late stages of anther development. | Zheng Tong Tingting Dong Qiuhua Li Rui Wang Junna He Bo Hong | 2024 | Horticultural Plant Journal2024，10，1： | 0 |
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