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| 1 | 植物转录因子与DNA互作研究技术显示文摘转录通过调控下游基因的时空特异性表达影响植物生长发育。在转录调控机制的解析过程中,转录因子与DNA的相互作用是关键的一环。近年来,研究者利用酵母单杂交(Y1H)和凝胶阻滞迁移率实验(EMSA)检测转录因子能否直接结合DNA;而瞬时表达技术则是一种检测转录因子对下游基因调控作用的便捷方式。该文对Y1H、EMSA和瞬时表达技术的原理、实验方法和相关注意事项进行详细阐述,以期为转录因子与DNA的互作研究提供参考方法。 | 杨立文 刘双荣 李玉红 林荣呈 | 2020 | 植物学报2020，55，4： | 2 |
| 2 | 香蕉基因MaPIF家族全基因组鉴定及激素表达模式显示文摘植物光敏色素作用因子(phytochrome interacting factor,PIF)是广泛分布于植物体内的一种转录因子,在植物的生长发育方面有着重要的作用.基于香蕉基因组数据,对香蕉MaPIF基因家族进行基因组鉴定,采用生物信息学分析方法对其进行命名,分析理化性质、蛋白质结构、基因结构、启动子顺势作用元件以及构建系统进化树;分析PIF家族在不同激素处理下的表达情况.结果显示,香蕉MaPIF家族有7个成员,均含有高度保守的bHLH结构域;编码区长度在1 116-2001bp之间,至少包含5个内含子,且大部分位于细胞外;进化树结果可以发现与拟南芥、水稻以及玉米PIF的亲缘关系较近;顺式作用元件预测结果显示,MaPIF上存在多种与激素和光相关的响应元件.qRT-PCR结果显示,MaPIF3-1、MaPIF4、MaPIF4-1在生长素(IAA)、赤霉素(GA)、生长素抑制剂(NPA)处理中均有显著表达,除此之外,所有成员在脱落酸(ABA)处理下均有明显表达.本研究表明MaPIF在香蕉生长发育中激素调控有重要作用.(图7表3参45) | 吴昊 张城瑜 倪珊珊 罗彬彬 王梦鸽 魏雯敏 陈裕坤 赖钟雄 | 2022 | 应用与环境生物学报2022，28，4： | 1 |
| 3 | Identification of a delayed leaf greening gene from a mutation of pummelo显示文摘Delayed greening of young leaves is an unusual phenomenon of plants in nature.Citrus are mostly evergreen tree species.Here,a natural mutant of“Guanxi”pummelo(Citrus maxima),which shows yellow leaves at the young stage,was characterized to identify the genes underlying the trait of delayed leaf greening in plants.A segregating population with this mutant as the seed parent and a normal genotype as the pollen parent was generated.Two DNA pools respectively from the leaves of segregating seedlings with extreme phenotypes of normal leaf greening and delayed leaf greening were collected for sequencing.Bulked segregant analysis(BSA)and In Del marker analysis demonstrated that the delayed leaf greening trait is governed by a 0.3 Mb candidate region on chromosome 6.Gene expression analysis further identified a key candidate gene(Citrus Delayed Greening gene 1,CDG1)in the 0.3 Mb region,which showed significantly differential expression between the genotypes with delayed and normal leaf greening phenotypes.There was a 67 bp In Del region difference in the CDG1 promoter and the In Del region contains a TATA-box element.Confocal laser-scanning microscopy revealed that the CDG1-GFP fusion protein signals were co-localized with the chloroplast signals in the protoplasts.Overexpression of CDG1 in tobacco and Arabidopsis led to the phenotype of delayed leaf greening.These results suggest that the CDG1 gene is involved in controlling the delayed leaf greening phenotype with important functions in chloroplast development. | Hui-Wen Yu Zhi-Hao Lu Xia Wang Dan Liu Jia-Xian He Xiao-Lin Jiang Ling-Jun Ke Wen-Wu Guo Xiu-Xin Deng Qiang Xu | 2021 | Science China(Life Sciences)2021，64，7： | 0 |
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