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| 1 | 南极罗斯海沉积物中可培养菌株的分离及胞外水解酶活性检测显示文摘研究了南极罗斯海地区沉积物中可培养微生物多样性,并检测了菌株胞外酶活性,为开发利用南极资源奠定基础。采用Zobell2216E培养基、土豆培养基(PDA)及稀释平板划线法分离并获得纯培养的细菌和真菌,分别通过16SrDNA、18S/ITS序列鉴定分析了可培养微生物的多样性和系统发育。从南极罗斯海沉积物样品中获得78株细菌和54株真菌,细菌、真菌的优势菌株分别为嗜冷杆菌属(Psychrobacter)、枝孢属(Cladosporium);78株细菌菌株中有2株与模式菌株的16SrDNA基因序列相似性小于97%,可能是潜在的细菌新物种。细菌API20NE生理生化和真菌产胞外酶活性结果表明,获得的细菌和真菌大多数可检测到胞外水解酶活性。本研究丰富了南极可培养微生物资源,为南极罗斯海地区可培养微生物的多样性研究以及南极微生物资源的应用研究奠定了基础。 | 王飞 丛柏林 张朝晖 杨黄浩 | 2020 | 极地研究2020，32，1： | 1 |
| 2 | Carbon isotope ratios of n-alkanoic acids: new organic proxies for paleo-productivity in Antarctic ponds显示文摘Primary productivity in the Antarctic aquatic environment with simple ecosystems is sensitive to climate and environmental fluctuations.We investigatedδ13C values for n-alkanoic acids derived from phototrophic organisms in a lacustrine sediment core(IIL3)to indicate primary productivity in ponds on Inexpressible Island in the western Ross Sea,Antarctica.Short-chain n-alkanoic acids(C14–C18)were abundant in the IIL3 sediment profile.The carbon isotope ratios of short-chain n-alkanoic acids in the sediment samples and floating microbial mats were similar,indicating that the short-chain n-alkanoic acids in the IIL3 sediment profile predominantly originated from phototrophic organisms.Theδ13C values for the short-chain n-alkanoic acids varied widely through the sediment profile,and 13C-enrichment of n-alkanoic acids was most likely related to high productivity due to carbon-limited conditions caused by enhanced photosynthetic efficiency.Theδ13C values for the n-alkanoic acids changed over the past 3200 years in similar ways to organic proxies for aquatic productivity(n-alkanoic acid and sterol sedimentary fluxes).C16 n-alkanoic acid was enriched in 13C in periods of high aquatic productivity~750–1650 and 3000–3200 a BP but depleted in 13C in periods of relatively low productivity~150–600 and 2500–3000 a BP.The results indicated that carbon isotope ratios of lipids from phototrophic organisms could be used as new proxies to reconstruct paleo-productivity in Antarctic lakes and ponds and therefore improve our understanding of past climate changes. | CHEN Xin JIN Jing NIE Yaguang ZHANG Jifeng DONG Liang HUANG Xianyu Steven D()EMSLIE LIU Xiaodong | 2023 | Advances in Polar Science2023，34，4： | 0 |
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