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1仿生合成氧化锆固定化漆酶处理孔雀石绿显示文摘以溶菌酶作为诱导剂,仿生合成了Zr O2固定化漆酶纳米颗粒,其酶活回收率达59%,采用场发射扫描电子显微镜(FESEM)、能谱仪(EDS)、热重分析仪(TGA)等手段对Zr O2纳米颗粒及Zr O2固定化漆酶颗粒进行表征,结果表明漆酶可成功固定到Zr O2颗粒中,同时还证明了溶菌酶既作为诱导剂催化Zr O2的形成,又作为生物模板同酶一起包埋在Zr O2颗粒中。固定化漆酶的最适pH为3,最适温度为70℃,相比于游离酶,其pH、温度稳定性都有明显提高;固定化漆酶纳米颗粒在4℃下储存30d,活性为初始酶活的95%,重复使用5次,固定化酶的残余酶活力仍有60%。此外,固定化漆酶在6h内对孔雀石绿染料的脱色率高达95%以上,通过紫外-可见吸收光谱分析(UV-vis)可知,固定化漆酶对孔雀石绿染料的处理是由吸附和降解联合作用引起的脱色。王美银 张新颖 林琳琳 李小龙 李宗仁 2018化工进展2018,37,7:1
2Degradation of 2,4-Dichlorophenol Catalyzed by Ultrasound-Assisted Laccase显示文摘Laccase possesses a good degradation effect on organic pollutants,but it is too long to achieve the desired effect. In order to improve the treatment effect of laccase on degradation of organic pollutants,2,4-dichlorophenol( 2, 4-DCP) was selected as a treatment target in the study. This study investigated a newtechnique for catalyzing the degradation of 2,4-DCP, that is,ultrasound-assisted laccase catalysis. The optimal experimental parameters such as p H,ultrasonic power,duty cycle and laccase concentration were determined under optimized experimental conditions. The results showed that the optimum conditions for degradation of 2,4-DCP were that pH = 5. 5,the input power was 105 W,the duty cycle was 50% and the laccase concentration was0. 4 U/m L. The degradation rate of 2,4-DCP reached 77. 5% under the optimum conditions at 4 h. When in ultrasonic environment,the enzymatic activity of laccase could be stimulated and improved.Compared with conventional methods,this technique significantly promoted the degradation rate of 2,4-DCP while reduced action time. Furthermore, no newpollutant was introduced into the degradation process. Therefore,ultrasound-assisted laccase catalysis is an environmentally friendly technique to degrade pollutants.郭慧文 张淑琴 任大军 邓志群 黄朝凡 康晨 2018Journal of Donghua University(English Edition)2018,35,6:0
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