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| 1 | Effect of lactic acid bacteria inoculants on alfalfa(Medicago sativa L.) silage quality:assessment of degradation(in situ) and gas production(in vitro)显示文摘Alfalfa(Medicago sativa) is difficult to ensile successfully because of the low content of moisture and water-soluble carbohydrates(WSC) in fresh alfalfa and the high buffering capacity in fresh alfalfa. Here, we conducted a study to evaluate the effects of three lactic acid bacteria(LAB) inoculants(Lactobacillus casei, Lactobacillus plantarum, and Pediococcus pentosaceus) on silage quality, in situ ruminal degradability, and in vitro fermentation of alfalfa silage. The first cut of alfalfa was wilted, chopped, and randomly divided into four groups: the control(CON) and control mixed with three separate LAB inoculants(10~6 cfug^(–1)). Simmental steers with a body weight of(452±18) kg and with installed rumen fistulas were prepared for in situ degradation and for in vitro gas production. LAB inoculants had a lower(P<0.05) content of butyric acid than the CON group. Among them, the L. casei inoculated silage had a higher(P<0.05) content of water-soluble carbohydrate(WSC) and a lower(P<0.05) NH_3-H content. The effective degradation(ED) of crude protein in LAB inoculation decreased(P<0.05), while the ED of acid detergent fiber increased(P<0.05) in situ fermentation. The alfalfa silage with LAB inoculants produced more carbon dioxide(P<0.05). The NH_3-H content of mixed incubation fluid in L. casei inoculated silage was lower(P<0.05) compared with other groups. Therefore, this study showed that LAB inoculants could improve both ensiling quality and degradation. In particular, the L. casei inoculations exhibited better performance by limiting proteolysis during ensiling. | LIU Ce LAI Yu-jiao LU Xiao-nan GUO Ping-ting LUO Hai-ling | 2016 | Journal of Integrative Agriculture2016，15，12： | 3 |
| 2 | 不同玉米秸秆NDF消化率的瘘管牛瘤胃微生物差异性研究显示文摘试验旨在对饲喂不同NDF消化率的玉米秸秆的牛瘤胃微生物进行差异分析。试验选取装有永久性瘘管的草原红牛6头,利用尼龙袋法进行瘤胃有效降解率的评价。试验牛随机分为2组,分别饲喂发酵玉米秸秆(添加复合菌剂)和青贮玉米秸秆(不添加复合菌剂),在饲喂第0、7、21 d采集瘤胃液,命名为青贮组(CSS0、CSS7、CSS21)和发酵组(FCS0、FCS7和FCS21)。结果显示,与青贮玉米秸秆相比,发酵玉米秸秆的干物质(DM)、酸性洗涤纤维(ADF)、中性洗涤纤维(NDF)的瘤胃有效降解率均显著提高(P<0.05)。饲喂发酵玉米秸秆的牛瘤胃细菌的多样性和丰富度显著提高(P<0.05)。在属水平上,共有9个具有显著性差异的菌属。FCS21组的Prevotella、Prevotellaceae UCG-003、Desulfovibrio的相对丰度显著高于CSS21组(P<0.05),Fibrobacter、Ruminobacter、Ruminococcaceae UCG-010、Ruminococcaceae NK4A214 group、Butyrivibrio、Ruminococcus的相对丰度显著低于CSS21组(P<0.05)。通过对组间差异菌属的相对丰度与NDF消化率进行相关性分析,发现玉米秸秆的NDF消化率与瘤胃微生物的相对丰度显著相关(P<0.05)。研究表明,发酵玉米秸秆NDF有效降解率显著高于青贮玉米秸秆;不同NDF消化率的玉米秸秆饲喂瘘管牛,其瘤胃微生物结构与组成存在显著差异。 | 田祖光 郭威 孙悦龙 贾师奇 杨子怡 胡晓林 郭晓军 | 2021 | 饲料研究2021，44，8： | 2 |
| 3 | Direct-fed microbial: Animal nutrition and reproduction responses显示文摘Manipulating the gastrointestinal microbial ecosystem to enhance animal performance and reproductive responses has been one of the main goals of animal science researchers and veterinarians.Recent restrictions to the use of antimicrobials as growth promoters led researchers to seek alternative practices that can show promise both from the standpoint of efficacy as well as from the practical and economic aspects. | Abdelfattah Z M Salem Nicolas Di Lorenzo | 2015 | Journal of Integrative Agriculture2015，14，3： | 0 |
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