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| 1 | Aluminium is essential for root growth and development of tea plants(Camellia sinensis)^∞显示文摘On acid soils,the trivalent aluminium ion(Al3+)predominates and is very rhizotoxic to most plant species.For some native plant species adapted to acid soils including tea(Camellia sinensis),Al3+has been regarded as a beneficial mineral element.In this study,we discovered that Al3+is actually essential for tea root growth and development in all the tested varieties.Aluminum ion promoted new root growth in five representative tea varieties with dose-dependent responses to Al3+availability.In the absence of Al3+,the tea plants failed to generate new roots,and the root tips were damaged within 1 d of Al deprivation.Struc-tural analysis of root tips demonstrated that Al was required for root meristem development and activity.In situ morin@staining of Al3+in roots revealed that Al mainly localized to nuclei in root meristem cells,but then gradually moved to the cytosol when Al3+was subsequently withdrawn.This movement of Al3+from nuclei to cytosols was accompanied by exacerbated DNA damage,which suggests that the nuclear-targeted Al primarily acts to maintain DNA integrity.Taken together,these results provide novel evidence that Al3+is essential for root growth in tea plants through maintenance of DNA integrity in meristematic cells. | Lili Sun Mengshi Zhang Xiaomei Liu Qianzhuo Mao Chen Shi Leon VKochian Hong Liao | 2020 | Journal of Integrative Plant Biology2020，62，7： | 12 |
| 2 | Nitric oxide alleviates aluminum-induced oxidative damage through regulating the ascorbateglutathione cycle in roots of wheat显示文摘The possible association with nitric oxide(NO) and ascorbate-glutathione(As A-GSH) cycle in regulating aluminum(Al) tolerance of wheat(Triticum aestivum L.) was investigated using two genotypes with different Al resistance. Exposure to Al inhibited root elongation, and triggered lipid peroxidation and oxidation of As A to dehydroascorbate and GSH to glutathione disulfide in wheat roots. Exogenous NO significantly increased endogenous NO levels, and subsequently alleviated Al-induced inhibition of root elongation and oxidation of As A and GSH to maintain the redox molecules in the reduced form in both wheat genotypes. Under Al stress, significantly increased activities and gene transcriptional levels of ascorbate peroxidase, glutathione reductase, and dehydroascorbate reductase,were observed in the root tips of the Al-tolerant genotype Jian-864. Nitric oxide application enhanced the activity and gene transcriptional level of these enzymes in both wheat genotypes. g-Glutamylcysteine synthetase was not significantly Researaffected by Al or NO, but NO treatments increased the activity of glutathione peroxidase and glutathione S-transferase to a greater extent than the Al-treated wheat seedlings. Proline was significantly decreased by Al, while it was not affected by NO.These results clearly suggest that NO protects wheat root against Al-induced oxidative stress, possibly through its regulation of the As A-GSH cycle. | Chengliang Sun Lijuan Liu Yan Yu Wenjing Liu Lingli Lu Chongwei Jin Xianyong Lin | 2015 | Journal of Integrative Plant Biology2015，57，6： | 10 |
| 3 | 铝胁迫下大豆根系有机酸积累的特性显示文摘为探讨铝胁迫对大豆根系有机酸累积的影响,以大豆品种粤春03-3为研究对象,通过水培方法进行不同时间的铝处理,分别测定大豆根系的相对生长率、以及距根尖0~2和2~4 cm根段的铝含量、苹果酸浓度、柠檬酸浓度和草酸浓度等。结果表明:铝处理明显抑制了大豆根系的生长。随着铝处理时间的延长,0~2 cm根段的铝含量明显降低,而2~4 cm根段的铝含量明显增加。另外,铝胁迫对不同根段的有机酸累积的影响也不同。铝处理6 h显著提高了大豆根系的苹果酸浓度,但对柠檬酸和草酸浓度均无明显影响。铝处理12 h后,0~2 cm根段中3种有机酸的浓度在铝处理和对照之间均无明显差异,但2~4 cm根段的苹果酸浓度和草酸浓度均明显高于对照。说明苹果酸和草酸在大豆根系铝毒的内部忍耐机制中起着重要作用。 | 田聪 张烁 粟畅 郭安静 白振龙 梁翠月 | 2017 | 大豆科学2017，36，2： | 9 |
| 4 | Alleviation by abscisic acid of Al toxicity in rice bean is not associated with citrate efflux but depends on ABI5-mediated signal transduction pathways显示文摘Under conditions of aluminum(Al) toxicity,which severely inhibits root growth in acidic soils, plants rapidly alter their gene expression to optimize physiological fitness for survival. Abscisic acid(ABA) has been suggested as a mediator between Al stress and gene expression, but the underlying mechanisms remain largely unknown. Here,we investigated ABA-mediated Al-stress responses, using integrated physiological and molecular biology approaches.We demonstrate that Al stress caused ABA accumulation in the root apex of rice bean(Vigna umbellata [Thunb.] Ohwi &Ohashi), which positively regulated Al tolerance. However,this was not associated with known Al-tolerance mechanisms. Transcriptomic analysis revealed that nearly one-third of the responsive genes were shared between the Al-stress and ABA treatments. We further identified a transcription factor, ABI5, as being positively involved in Al tolerance. Arabidopsis abi5 mutants displayed increased sensitivity to Al, which was not related to the regulation of AtALMT1 and AtMATE expression. Functional categorization of ABI5-mediated genes revealed the importance of cell wall modification and osmoregulation in Al tolerance, a finding supported by osmotic stress treatment on Al tolerance. Our results suggest that ABA signal transduction pathways provide an additional layer of regulatory control over Al tolerance in plants. | Wei Fan Jia Meng Xu Pei Wu Zhi Xin Yang He Qiang Lou Wei Wei Chen Jian Fen Jin Shao Jian Zheng Jian Li Yang | 2019 | Journal of Integrative Plant Biology2019，61，2： | 9 |
| 5 | 铝胁迫下质膜H^+-ATPase对水稻硝态氮吸收的影响显示文摘为了研究铝胁迫下水稻质膜H^+-ATPase对硝态氮吸收影响,以‘滇优35号’(粳稻)为试验材料,采用溶液培养法研究50μmol·L^(-1) Al^(3+)胁迫下添加质膜H^+-ATPase的激活剂壳梭孢素(FC)和抑制剂腺苷-5’-单磷酸(AMP)对质膜H^+-ATPase水解活性和H^+-泵活性,质膜H^+-ATPase和14-3-3蛋白的相互作用对水稻NO_3^--N吸收的影响。结果表明,AMP处理使铝胁迫下水稻质膜H^+-ATPase水解活性、H^+-泵的活性,质膜H^+-ATPase和14-3-3蛋白的相互作用下降,H_2O_2和MDA含量增加,从而导致水稻NO_3^--N吸收降低。FC处理可使铝胁迫下水稻质膜H^+-ATPase水解活性、H^+-泵的活性以及质膜H^+-ATPase和14-3-3蛋白的相互作用增加,H_2O_2和MDA含量下降,从而使水稻NO_3^--N吸收增加。这些研究结果表明铝胁迫通过影响质膜H^+-ATPase与14-3-3蛋白互作来影响质膜H^+-ATPase水解活性和氢泵活性以及H_2O_2和MDA含量,进而影响对硝态氮的吸收。 | 周小华 徐慧妮 谷照虎 陈丽梅 李昆志 | 2016 | 植物生理学报2016，52，7： | 9 |
| 6 | 外源添加生物炭对水稻产量和土壤性质的影响显示文摘采用盆栽试验,研究不同用量秸秆生物炭(CK、1%、2%和4%)对水稻产量及其构成因素和土壤性质的影响。结果表明,外源添加生物炭显著提高了水稻产量,且水稻产量随着生物炭用量的增加而增加;添加生物炭有效提高了土壤pH、显著降低了土壤交换性酸和交换性铝,且生物炭施用越多,效果越明显;外源添加生物炭对土壤塑性指数无显著影响,但添加较多生物炭(4%)显著降低了土壤抗压强度。由此可见,外源添加生物炭不但可以增加水稻产量,而且有效改善了土壤性状。 | 刘晓霞 吴东涛 朱伟锋 陶云彬 王军君 陈一定 | 2016 | 浙江农业科学2016，57，11： | 7 |
| 7 | 云南2种野生苜蓿资源对酸铝胁迫的响应分析显示文摘以云南2种野生苜蓿资源为材料,采用水培法研究酸铝胁迫对幼苗生长的影响。通过分析酸、铝单一及复合处理下幼苗根长、株高、生物量、根系活力、叶绿素和丙二醛含量、过氧化物酶和超氧化物岐化酶活性变化,比较2种苜蓿对酸、铝的耐性。结果显示:随着酸度的增加,天蓝苜蓿和紫花苜蓿幼苗的生长均受抑制,pH 7.0和pH 3.0处理对比分析,天蓝苜蓿在根长、株高、生物量、根系活力增幅比紫花苜蓿的增幅小;铝胁迫后,2种苜蓿在根长、株高、生物量、根系活力指标上均具有显著差异,且随着铝浓度的增大,受抑制越严重;酸、铝共同胁迫对2种苜蓿幼苗生长的影响大于单一胁迫对它的影响,pH 4.5,Al3+浓度300 mg/L时,2种苜蓿生长受抑最严重。酸、铝单一胁迫及复合处理均显著影响2种苜蓿,但天蓝苜蓿对酸、铝及酸铝的耐性优于紫花苜蓿。 | 吴丽芳 魏晓梅 张丽芳 桂宝林 | 2020 | 热带作物学报2020，41，3： | 7 |
| 8 | 铝胁迫下外源抗坏血酸对水稻幼苗抗氧化性能的影响显示文摘为探讨外源抗坏血酸(AsA)对铝胁迫下水稻抗氧化损伤的效应,以滇优35号(杂交稻,粳稻)为试验材料,采用溶液培养法研究外源AsA对不同铝浓度(0、50、100、200、400μmol·L^-1)胁迫下水稻根尖H2O2含量及抗氧化酶活性等生理生化指标的影响。结果表明,水稻根尖氧化损伤程度随铝浓度的递增而加剧,与对照相比,400μmol·L-1铝胁迫可导致水稻根尖H2O2和MDA含量增加1.53和3.16倍,脯氨酸含量、SOD、POD、APX和CAT活性分别增加1.73、1.39、1.42、1.76和1.56倍,内源AsA含量减少0.53倍;而施用外源AsA处理的根尖H2O2和MDA含量只增加1.04和2.69倍,内源AsA含量减少0.46倍,脯氨酸含量增加1.96倍,SOD、POD、APX和CAT活性分别增加1.48、1.63、1.90和1.89倍。综上所述,外源AsA可通过增强渗透调节物质含量来维护质膜结构的完整性,增强抗氧化酶活性和内源AsA含量,降低根尖H2O2的积累和质膜过氧化程度,促进水稻在铝胁迫条件下的生长。本研究结果为进一步探索外源AsA缓解水稻铝毒的机理提供了一定的理论依据。 | 周小华 周泽仪 李昆志 | 2020 | 核农学报2020，34，10： | 7 |
| 9 | Characterization of the soybean Gm ALMT family genes and the function of Gm ALMT5 in response to phosphate starvation显示文摘A potential mechanism to enhance utilization of sparingly soluble forms of phosphorus(P) is the root secretion of malate, which is mainly mediated by the ALMT gene family in plants. In this study, a total of 34 Gm ALMT genes were identified in the soybean genome. Expression patterns diverged considerably among Gm ALMTs in response to phosphate(Pi) starvation in leaves, roots and flowers, with expression altered by P availability in 26 of the 34 Gm ALMTs. One root-specific Gm ALMT whose expression was significantly enhanced by Pi-starvation,Gm ALMT_5, was studied in more detail to determine its possible role in soybean P nutrition. Analysis of Gm ALMT_5 tissue expression patterns, subcellular localization, and malate exudation from transgenic soybean hairy rootsoverexpressing Gm ALMT_5, demonstrated that Gm ALMT_5 is a plasma membrane protein that mediates malate efflux from roots. Furthermore, both growth and P content of transgenic Arabidopsis overexpressing Gm ALMT_5 were significantly increased when sparingly soluble Ca-P was used as the external P source. Taken together, these results indicate that members of the soybean Gm ALMT gene family exhibit diverse responses to Pi starvation. One member of this family, Gm ALMT_5, might contribute to soybean P efficiency by enhancing utilization of sparingly soluble P sources under P limited conditions. | Wenting Peng Weiwei Wu Junchu Peng Jiaojiao Li Yan Lin Yanan Wang Jiang Tian Lili Sun Cuiyue Liang Hong Liao | 2018 | Journal of Integrative Plant Biology2018，60，3： | 7 |
| 10 | A WRKY transcription factor confers aluminum tolerance via regulation of cell wall modifying genes显示文摘Modification of cell wall properties has been considered as one of the determinants that confer aluminum(Al)tolerance in plants,while how cell wall modifying processes are regulated remains elusive.Here,we present a WRKY transcription factor WRKY47 involved in Al tolerance and root growth.Lack of WRKY47 significantly reduces,while overexpression of it increases Al tolerance.We show that lack of WRKY47 substantially affects subcellular Al distribution in the root,with Al content decreased in apoplast and increased in symplast,which is attributed to the reduced cell wall Al-binding capacity conferred by the decreased content of hemicellulose I in the wrky47-1 mutant.Based on microarray,real time-quantitative polymerase chain reaction and chromatin immunoprecipitation assays,we further show that WRKY47 directly regulates the expression of EXTENSIN-LIKE PROTEIN(ELP)and XYLOGLUCAN ENDOTRANSGLUCOSYLASE-HYDROLASES17(XTH17)responsible for cell wall modification.Increasing the expression of ELP and XTH17 rescued Al tolerance as well as root growth in wrky47-1 mutant.In summary,our results demonstrate that WRKY47 is required for root growth under both normal and Al stress conditions via direct regulation of cell wall modification genes,and that the balance of Al distribution between root apoplast and symplast conferred by WRKY47 is important for Al tolerance. | Chun Xiao Li Jing Ying Yan Jiang Yuan Ren Li Sun Chen Xu Gui Xin Li Zhong Jie Ding Shao Jian Zheng | 2020 | Journal of Integrative Plant Biology2020，62，8： | 7 |
| 11 | 不同基因型油菜耐铝性及其根系形态对铝胁迫的响应显示文摘本研究旨在为酸性土壤地区筛选耐铝毒的甘蓝型油菜,探讨不同基因型油菜苗期生长发育指标和生理指标的耐铝特性,分析铝胁迫对根系形态的影响。以81个油菜品种为材料,通过水培试验,设置铝浓度(AlCl3)0、100μmol·L-1两个处理,对根系形态、株型、生物量、生理特性等13指标的耐铝系数进行相关分析、主成分分析和聚类分析,采用隶属函数法综合评价不同基因型油菜的耐铝相关指标,并分析不同耐铝类型的品种根系形态差异。结果表明,铝胁迫对不同基因型油菜苗期各单项指标有不同程度的影响,综合应用主成分分析、聚类分析和隶属函数法筛选出耐铝性较强的品种6个,耐铝性中等的品种65个,耐铝性弱的品种10个。不同直径范围根系对铝胁迫的响应不同,铝胁迫下,D1类根系(直径0.0~0.5 mm)的长度、表面积、体积、根尖数降幅最大,D3类根系(直径>2.0mm)各指标降幅最小,且铝敏感型品种不同类别的根系指标受铝胁迫的抑制程度大于耐铝型品种,其D1、D2(直径0.5~2.0 mm)、D3类根系的长度、表面积、体积、根尖数均少于耐铝型品种。因此,以综合指标对油菜耐铝性进行筛选与评价更为客观;耐铝型品种不同直径范围的根系受铝毒害的影响较轻,通过改变根系形态适应铝胁迫的能力更强,其根系拥有更多细根可能是耐性品种抗铝毒的一种作用机制。 | 熊洁 丁戈 陈伦林 李书宇 邹小云 黄杨 宋来强 | 2021 | 中国油料作物学报2021，43，4： | 6 |
| 12 | 基于转录组测序的铝胁迫下甘蓝型油菜新内参基因的发掘与引物开发显示文摘为了研究甘蓝型油菜在铝胁迫条件下内参基因的稳定性,在受到主要铝毒害的根组织内发掘新内参基因,以甘蓝型油菜耐铝品种赣油杂7号和铝敏感品种蓉油18的苗期根组织为研究材料,对营养液培养的试验材料分别设置对照组(0 h)和2个时间梯度(3,24 h)的铝胁迫(100μmol/L AlCl_(3),pH值4.5)处理组。首先,采用有参考基因组的转录组测序技术(RNA-seq)及FPKM值差异基因表达分析,筛选出符合持家基因特征的5个候选内参基因(Actin7、ARFA1E、SAP5、UXS3、UBC9)。随后,依次进行实时荧光相对定量PCR分析,扩增曲线和熔解曲线分析,LinRegPCR扩增效率分析,geNorm、NormFinder和BestKeeper基因表达稳定性分析,REST 2009相对表达量分析等分析步骤。结果表明,新设计开发的5对候选内参基因引物特异性高。从转录组测序数据中筛选出的4个候选内参基因(Actin7、ARFA1E、SAP5、UXS3)的表达稳定性获得了验证。按表达稳定性的综合排名由高至低排序依次为UXS3、SAP5、ARFA1E、Actin7,其中最优组合为SAP5和UXS3。综上所述,本研究开发的候选内参基因引物特异性高,可以提高铝胁迫试验体系实时荧光相对定量PCR试验的准确性,新发掘的内参基因(Actin7、ARFA1E、SAP5、UXS3),可以作为铝胁迫试验体系实时荧光相对定量PCR试验的新内参基因。 | 丁戈 黄杨 陈伦林 李书宇 宋来强 熊洁 | 2021 | 华北农学报2021，36，1： | 5 |
| 13 | 外源抗坏血酸缓解水稻幼苗的铝胁迫效应显示文摘为探讨外源抗坏血酸对铝胁迫下水稻氧化损伤的影响,该文以峰1A(不育系,籼稻)和滇优35号(杂交,粳稻)两个水稻品种为材料,采用溶液培养法研究外源抗坏血酸(AsA)对50μmol·L-1Al3+胁迫下水稻根尖H2O2和内源AsA含量以及抗氧化酶活性的影响。结果表明:铝胁迫24 h时,水稻根尖内源AsA含量为对照的0.90倍,与对照相比,根尖H2O2增加了0.55倍至1.3倍,SOD、POD、CAT及APX活性分别增加了0.83倍至1.6倍、1.0倍至1.3倍、0.85倍至1.2倍、1.0倍至1.8倍;外源AsA处理铝胁迫水稻24 h,水稻根尖内源AsA含量为对照的1.2倍,SOD、POD、CAT及APX活性分别增加了1.2倍至2.2倍、1.5倍至1.6倍、1.3倍至1.7倍、1.2倍至2.6倍,根尖H2O2含量增加了0.1倍至0.6倍。这些研究结果说明铝胁迫加剧了细胞膜的过氧化程度,并诱导抗氧化酶活性以清除活性氧,外源AsA通过提高抗氧化酶活性和内源AsA含量以缓解水稻Al3+胁迫引起的氧化损伤,降低细胞膜系统伤害,促进水稻生长,为外源AsA提高水稻适应铝胁迫能力提供了理论依据。 | 周小华 周泽仪 李昆志 | 2020 | 广西植物2020，40，4： | 4 |
| 14 | 铝胁迫对不同耐铝油菜品种苗期生长发育和养分吸收的影响显示文摘为探明铝胁迫对油菜生长发育和养分吸收特性的影响,以2个耐铝性差异显著的油菜品种为试验材料,采用营养液培养,设置4个铝胁迫浓度和2个胁迫时间,测定根系形态指标(总根长、总根表面积、总根体积、总根尖数)、生长指标(根茎粗、株高、地上干质量、根干质量)和营养元素(N、P、K、Ca、Mg、Fe、Al)的含量。结果表明,铝胁迫降低了油菜总根长、总根表面积、总根体积、总根尖数以及根茎粗、株高、根干质量、地上干质量,且随着铝浓度的增加和胁迫时间的延长降幅增大,敏感型品种降幅大于耐铝型品种。铝胁迫对油菜地上部和根系中铝元素的吸收具有促进作用,其中根系铝含量增幅高于地上部;而对N、P、K、Ca、Mg、Fe等元素的吸收有抑制作用。随着铝胁迫浓度增加和胁迫时间延长各元素含量的增幅或降幅增大,且基因型之间存在着一定的差异,敏感型品种的变化幅度大于耐铝型品种。由此可见,与敏感型品种相比,耐铝型油菜品种的根系形态、地上部生长和营养元素吸收受铝毒害的抑制作用较小,其对铝毒害有较强的适应能力。研究结果可为酸性土壤上的油菜高产栽培提供理论依据。 | 熊洁 丁戈 李书宇 陈伦林 宋来强 | 2020 | 华北农学报2020，35，6： | 4 |
| 15 | 不同水稻品种磷利用效率与耐铝性的关系研究显示文摘铝毒和磷缺乏是酸性土壤上作物生产的主要限制因子。本研究中我们探究了5个粳稻和5个籼稻品种的磷利用效率和耐铝性之间的相互关系。结果表明,粳稻品种的耐铝性显著高于籼稻品种。对于耐铝性强的水稻品种,施加磷肥后地上部生物量显著增加,而铝敏感的品种对磷肥响应较小,这可能是由于其耐铝性差而酸性土壤中的铝毒导致根系结构和功能受损,从而影响养分的吸收和利用。不同水稻品种的耐铝性和磷吸收效率呈正比而与磷利用效率呈反比,且粳稻的地上部磷浓度及磷吸收效率高于籼稻,但磷利用效率则低于籼稻。这些结果对于酸性土壤中筛选耐铝和磷高效利用的水稻品种具有重要意义。 | 胡安永 赵学强 沈仁芳 马建锋 | 2020 | 土壤2020，52，1： | 2 |
| 16 | SIZ1 negatively regulates aluminum resistance by mediating the STOP1–ALMT1 pathway in Arabidopsis显示文摘Sensitive to proton rhizotoxicity 1(STOP1) functions as a crucial regulator of root growth during aluminum(Al) stress. However, how this transcription factor is regulated by Al stress to affect downstream genes expression is not well understood. To explore the underlying mechanisms of the function and regulation of STOP1, we employed a yeast two hybrid screen to identify STOP1-interacting proteins. The SUMO E3 ligase SIZ1, was found to interact with STOP1 and mainly facilitate its SUMO modification at K40 and K212 residues. Simultaneous introduction of K40 R and K212 R substitutions in STOP1 enhances its transactivation activity to upregulate the expression of aluminum-activated malate transporter 1(ALMT1)via increasing the association with mediator 16(MED16) transcriptional co-activator. Loss of function of SIZ1 causes highly increased expression of ALMT1, thus enhancing Al-induced malate exudation and Al tolerance. Also, we found that the protein level of SIZ1 is reduced in response to Al stress. Genetic evidence demonstrates that STOP1/ALMT1 is epistatic to SIZ1 in regulating root growth response to Al stress. This study suggests a mechanism about how the SIZ1–STOP1–ALMT1 signaling module is involved in root growth response to Al stress. | Jiameng Xu Jiayong Zhu Jiajia Liu Junxia Wang Zhaojun Ding Huiyu Tian | 2021 | Journal of Integrative Plant Biology2021，63，6： | 2 |
| 17 | 利用分子标记辅助选择技术创制耐铝玉米种质显示文摘【目的】将耐铝主效基因导入普通玉米自交系,筛选出耐铝玉米株系,为拓宽玉米耐铝种质资源及耐铝育种提供理论参考。【方法】以玉米耐铝材料(CML530、CML532、CML533和CML534)和普通玉米自交系(西1、9058和LX9801)为材料,构建6个回交群体,基于分子标记辅助选择技术,利用与耐铝基因Zm M1、Zm M2、Zm ASL和Zm ALMT2紧密连锁的分子标记(umc1468、ZmMATE1、ZmMATE2、MateF2和ALMT93496)进行靶基因选择,将耐铝主效基因导入到普通玉米自交系,结合植株田间表现,筛选出耐铝的稳定株系,并对其进行耐铝鉴定。【结果】在分离世代(BC1F1/S1)苗期对6个回交群体共581个株系进行靶基因选择,结果有69个株系中选,结合植株田间表现,最终获得17个稳定株系。基于分子标记检测结果,17个稳定株系的遗传背景恢复率为73.9%~93.8%,平均为83.4%。0.2 mmol/L AlCl3溶液处理下17个稳定株系的根系生长均受到不同程度的抑制,根净增长度平均值为0.81 cm,极显著低于对照(0.5 mmol/L CaCl2溶液)(P<0.01,下同);稳定株系的根相对伸长率与轮回亲本差异达极显著水平;17个稳定株系的根相对伸长率为64.50%~83.33%,平均为74.74%,均为中等及以上耐铝株系,其中NS1、NS9、NS10、NS15和NS16为高耐铝株系。【结论】通过分子标记辅助选择技术可将耐铝主效基因导入普通玉米自交系,且导入多个耐铝基因株系的耐铝性较同一群体中导入单个耐铝基因的株系均有不同程度提高,表明多个耐铝基因累加可提高耐铝性。可见,该技术可作为耐铝玉米种质创制的有效手段。 | 邓磊 潘金卫 杨文鹏 任洪 王伟 柏光晓 | 2021 | 南方农业学报2021，52，4： | 1 |
| 18 | 铝胁迫下马尾松二代家系苗生长规律与筛选显示文摘[目的]铝胁迫是限制植物生长的重要非生物胁迫因子,严重制约我国南方酸性土壤森林生产力水平。为探明酸铝环境下马尾松幼苗生长变异规律,筛选出适应酸性环境耐铝毒家系和敏感性家系,为马尾松耐铝胁迫遗传改良提供依据。[方法]以55个马尾松二代优良家系(201~255)幼苗为材料,采用沙培方式研究酸性环境(pH=4.0)不同铝浓度(0、0.1、0.3、0.6、1.2、2.4 mmol·L^(−1))马尾松苗生长和根系形态变化规律,并通过主成分分析和聚类分析对马尾松耐铝特性进行分等定级,筛选和鉴定马尾松幼苗耐铝优良家系。[结果]苗高、地径、高径比、生物量(地上生物量、根生物量、总生物量、根冠比)、根系形态(总根长、根表面积、根体积)在家系间和铝浓度处理间均存在显著差异(p<0.05)。随铝浓度增加马尾松幼苗地径、苗高、生物量和根系形态指标均呈现先升后降趋势,在0.6 mmol·L^(−1)铝浓度下最大,显著高于0、2.4 mmol·L^(−1)处理,而与0.3 mmol·L^(−1)处理无显著差异,其中根生物量、总根长、根表面积和根体积的变异系数较大,平均为30.79%,属中等变异,根系可作为马尾松幼苗适应酸铝环境敏感性器官。用上述10个指标计算家系间耐铝特性综合评价值(CEV)和系统聚类,筛选出强度耐铝型家系3个(占5.45%),中度耐铝型家系11个(占20%),一般耐铝型家系37个(占67.28%),铝敏感型家系4个(占7.27%)。[结论]适宜铝浓度能够促进马尾松生长,尤其是0.3~0.6 mmol·L^(−1)铝浓度下促进效果佳,筛选得到耐铝型和敏感型家系对马尾松耐铝性状改良具有重要意义。 | 李鹏 谭健晖 杨章旗 罗群凤 凌金燕 陈虎 陈钊铭 贾婕 | 2023 | 林业科学研究2023，36，5： | 0 |
| 19 | AtPIN2基因通过影响根尖抗氧化能力参与拟南芥耐铝响应显示文摘PIN2是根尖生长素运输的重要蛋白,PIN2基因的差异表达是否影响植物耐铝性并不清楚。本文以不同AtPIN2表达水平的拟南芥为材料,研究了AtPIN2差异表达对拟南芥耐铝性的影响。结果表明,铝处理明显增强拟南芥根尖0-5 mm区域AtPIN2基因的转录表达,与对照相比,其表达量增加了80%。铝能诱导PIN2蛋白在根尖细胞膜水平方向累积,并且呈束状分布。通过分析不同AtPIN2表达材料的耐铝性,发现在铝处理条件下,PIN2缺失突变体(pin2-ko)的根系伸长速率显著小于PIN2超表达(PIN2-OX)和野生型材料(Col);并且,pin2-ko累积较多的活性氧;pin2-ko的SOD、CAT和APX活性以及GSH含量均显著低于PIN2-OX。上述结果表明,AtPIN2表达差异影响拟南芥耐铝性,敲除AtPIN2导致其对铝毒更敏感,其机理可能是AtPIN2通过影响根尖抗氧化能力参与拟南芥耐铝响应。 | 曹华苹 吴道铭 沈宏 | 2015 | 植物生理学报2015，51，3： | 0 |
| 20 | 水稻和小麦根尖细胞壁多糖的铝积累能力比较显示文摘采用水培法比较4种禾本科植物水稻(Oryza sativa L.)、玉米(Zea mays L.)、高粱(Sorghum bicolor(L.) Moench)和小麦(Triticum aestivum L.) 8个基因型的抗铝(Al)能力,并对他们在Al积累后细胞壁的多糖组分进行分析。结果显示,在5~200μmol/L Al处理下,水稻抗Al能力较强,而小麦抗Al能力较弱。在50μmol/L Al处理下,小麦根尖的果胶和半纤维素1含量的增幅明显高于水稻。水稻基因型‘日本晴’与‘浙辐802’的细胞壁Al含量分别占根尖总Al含量的78. 7%和91. 6%;小麦基因型‘扬麦18’与‘扬麦16’Al含量分别占根尖总Al含量的64.9%和72.1%。Al吸附-解吸实验结果显示,小麦根尖细胞壁上Al的吸附量高于水稻。研究结果表明,细胞壁是Al积累的主要部位,对Al敏感的水稻和小麦基因型细胞壁中的Al主要分布在果胶中;而对Al耐性较强的水稻和小麦基因型细胞壁中的Al主要分布在半纤维素1中。 | 刘庆 王智怡 吴坤 邢承华 常虹 饶玉春 蔡妙珍 | 2019 | 植物科学学报2019，37，4： | 0 |
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