| 1 | 不同配方高脂饲料构建SD大鼠肥胖模型的实验研究显示文摘目的:研究不同配方高脂饲料对构建SD大鼠肥胖模型成功率的影响。方法:40只5周龄雄性SD大鼠随机分成4组:C组(SPF级繁殖鼠料),H1组(40%自配高脂饲料)、H2组(45%高脂饲料)、H3组(60%高脂饲料),每组10只。每周称量大鼠体重,饲养11周后大鼠麻醉处死,测量每只大鼠的体长、脂肪重量(肾周脂肪和附睾脂肪),计算其脂体比、BMI和Lee’s指数;检测大鼠的血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)浓度。分析各组间上述指标的差异性,综合评估不同配方饲料对SD大鼠肥胖的影响。结果:(1)H2组大鼠自第8周体重显著高于对照组(P<0.05),且建模效率最高;(2)45%高脂饲料显著升高大鼠血清TC、TG和LDL-C浓度,而显著降低HDL-C浓度(P<0.05);(3)60%高脂饲料极显著升高大鼠血清TC和TG浓度(P<0.01),显著升高LDL-C浓度(P<0.05),而极显著降低HDL浓度(P<0.01);H2和H3组大鼠肾周脂肪重量、附睾脂肪重量、脂体比和Lee’s指数显著高于对照组。结论:(1)构建SD大鼠肥胖模型成功的判断标准:高脂饲料组体重显著增加,TC、TG、LDL-C显著升高,HDL-C显著降低,脂体比显著增加,Lee’s指数显著升高;且个体体重超过对照组平均体重20%。(2)喂饲6周是利用45%脂供能高脂饲料构建SD大鼠肥胖模型最经济的建模周期。 | 朱磊 路瑛丽 冯连世 张辉 | 2016 | 中国运动医学杂志2016，35，7： | 38 |
| 2 | 绵羊UCP2基因的克隆及其在脂肪组织中的季节性差异表达显示文摘旨在研究绵羊UCP2基因的克隆和在脂肪组织中的季节性表达差异,以揭示季节对UCP2基因表达的调控作用。本研究采用RT-PCR和RACE法克隆UCP2cDNA全长序列。以南非肉用美利奴和山西肉用绵羊为对象,用real-time PCR和免疫组化技术检测UCP2mRNA及其编码蛋白在冬春和夏秋2个阶段6种脂肪组织(皮下脂肪、肠系膜、大网膜、小网膜、腹膜后脂肪和肾周脂肪)中的差异表达。结果表明,绵羊UCP2cDNA全长1 641bp,包括8个外显子,开放阅读框930bp,编码309个氨基酸。UCP2mRNA在6种脂肪组织中均有表达,其中肾周脂肪中的表达量最高,皮下脂肪中最低,即深层脂肪中的表达量高于浅层脂肪(P<0.05)。UCP2蛋白的组织表达趋势与mRNA相似,且均分布于细胞膜上。二者的表达均受季节的显著影响,冬春阶段的表达量高于夏秋季节(P<0.05)。这些结果说明绵羊脂肪组织中UCP2基因的表达与季节有关,以维持体温和调节机体能量平衡。 | 刘建华 乔利英 郭云雁 贾夏丽 刘文忠 | 2015 | 畜牧兽医学报2015，46，7： | 5 |
| 3 | Comparative study on the difference of obesity model induced by two kinds of high fat diet in Sprague-Dawley rats显示文摘Objective: To explore the differences of obese Sprague-Dawley(SD)rats model induced by lard oil high-fat(HF)diet or purified HF diet. Methods: SD weanling rats were randomly divided into three groups: D1 group,where rats were fed by lard oil HF diet;D2 group,where rats were fed by purified HF diet;C group,where rats were fed on chow. After 12 weeks,diet-induced obesity rat(stop 33% based on weight)were selected for further study,and the rest rats from group D1 and D2 were excluded. The food intake and weight were weighted daily and weekly,respectively. The subcutaneous,visceral and total fat contents of rats was measured by 256-row CT scan and the Lee index was calculated accordingly. The kidney,liver,testis,spleen and heart were weighted respectively. Serum leptin and insulin levels were quantified. The pathology in liver and adipose tissues were analyzed by HE staining. Oral glucose tolerance test(OGTT)was used to compare the glucose tolerance ability. Serum total cholestero(lT-CHO),high density lipoprotein(HDL-C),low density lipoprotein(LDL-C),triglyceride(TG)and inflammatory cytokines IL-6,TNF-α were detected as well. Results: After 12 weeks,the body weight,subcutaneous fat,visceral fat,total fat mass,wet weight of liver,kidney and heart,area under blood glucose curve and the levels of serum insulin,leptin,T-CHO,LDL-C,TG,IL-6 and TNF-α in group D2 were significantly increased compared to those of group C and group D1. HDL-C of group D2 was markedly lower than that in group C(P<0. 05). The visceral fat,total fat content and HDL-C in group D1 were significantly different from those of group C(P<0. 05). Steatosis and enlarged adipocyte were found in the livers of rats in group D1 and D2,and the lesions were more significant in group D2. Conclusion: Purified HF diet was more effective in inducing metabolic abnormalities,steatosis,peripheral chronic inflammation in obese SD rat models. But lard oil HF diet was more economical when only inducing visceral steatosis was required. | Shanlei Zhang Wenqing Mo Xi Rong Fang Wei Hong Liu Fuling Huang | 2018 | 广西医科大学学报2018，35，1： | 0 |
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