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| 1 | p38MAPK抑制剂SB202190对猪颗粒细胞活力的影响显示文摘p38MAPK是丝裂原活化蛋白激酶(Mitogen-activated potein kinase,MAPK)家族成员,是细胞内重要的信号转导系统之一,对细胞的生长、分化、凋亡具有重要影响及作用。为探究p38MAPK信号对猪卵巢颗粒细胞增殖和凋亡的影响及其可能作用机制,研究以体外培养的猪卵巢颗粒细胞为模型,在培养液中分别添加浓度为0、1、10、20μmol/L的p38MAPK特异性抑制剂SB202190。以免疫细胞化学染色法鉴定体外培养猪卵巢颗粒细胞,通过CCK-8法检测其增殖活性,应用Annwxin V-FITC/PI及细胞周期试剂盒染色检测细胞凋亡及细胞周期状况,以实时定量PCR检测猪卵巢颗粒细胞中凋亡、周期相关基因的相对表达水平。结果表明,添加p38APK信号抑制剂SB202190可抑制体外培养猪卵巢颗粒细胞的增殖活性,20μmol/L处理组与其他组相比呈显著性差异(P<0.05);20μmol/L SB202190添加处理未显著影响颗粒细胞的凋亡水平,而该浓度SB202190可将大部分细胞阻滞在G1/G0期,与对照组相比差异显著(P<0.05);基因相对表达水平检测显示SB202190添加可抑制Fas、FasL、CDK-4的表达,而同时促进Bcl-2、Bax、P27的表达(P<0.05),对Caspase-3的表达没显著影响(P>0.05)。综上所述,SB202190通过调控猪卵巢颗粒细胞的周期来影响其增殖作用,但对其凋亡没有影响。 | 石美虹 耿果霞 贺亚媚 程建勇 李青旺 | 2016 | 家畜生态学报2016，37，9： | 2 |
| 2 | 不同浓度β-巯基乙醇对6周龄杜泊羊卵母细胞发育能力的影响显示文摘为了探讨成熟液里不同浓度β-巯基乙醇对6周龄杜泊羊卵母细胞发育能力的影响,试验选择6只6周龄杜泊羊,采用促性腺激素诱导活体采集卵母细胞,将获得的A、B级卵丘-卵母细胞复合体(COCs)在不同浓度(0、50、70、100μmol/L)β-巯基乙醇成熟液中成熟,再经成熟卵母细胞与获能精子共同孵育、受精卵发育培养后,统计卵裂率和囊胚率。结果表明:与对照组卵裂率(59.13%)和囊胚率(12.17%)相比,试验Ⅰ、Ⅱ、Ⅲ组的卵裂率(60.87%、63.48%、65.22%)和囊胚率(14.78%、17.39%、21.74%)均较高(P>0.05),其大小顺序为试验Ⅲ组>试验Ⅱ组>试验Ⅰ组>对照组,100μmol/Lβ-巯基乙醇组的卵裂率、囊胚率分别增加6.09个百分点和9.57个百分点。说明成熟液里添加一定量β-巯基乙醇能够提高6周龄杜泊羊卵母细胞的发育能力和受精卵质量,β-巯基乙醇浓度在100μmol/L以内,卵母细胞的卵裂率和囊胚率呈上升趋势;关于成熟液里β-巯基乙醇最佳浓度,有待进一步研究。 | 毛杨毅 罗惠娣 王志武 赵鹏 张冠武 李俊 郭宏宇 张丽 薛丽娜 党文庆 | 2018 | 中国草食动物科学2018，38，3： | 1 |
| 3 | Effects of β-mercaptoethanol Concentrations on Developmental Ability of Oocytes in 6-week-old Dorper Sheep显示文摘This study was conducted to investigate the effects of different concentrations ofβ-mercaptoethanol on the development ability of oocytes in Dorper sheep at the age of 6 weeks.In this study,6 Dorper sheep of 6 weeks old were chosen to induce oocyte collection in vivo by gonadotropin,and A and B grade cumulus oocyte complexes were obtained(COCs)with different concentrations of(0,50,70 and 100μmol/L)ofβ-mercaptoethanol in the mature liquid.The cleavage rate and blastocyst rate were counted after matured oocytes and capacitated sperm were co-incubated and fertilized eggs were cultivated.The results showed that compared with the cleavage rate(59.13%)and the blastocyst rate(12.17%)of the control group,the cleavage rates(60.87%,63.48%and 65.22%)and blastocyst rates(14.78%,17.39%and 21.74%)of the test groups I,II and III were higher(P>0.05),and the order was test group III>test group II>test group I>control group.The cleavage rate and blastocyst rate of the 100μmol/Lβ-mercaptoethanol group increased by 6.09 percentage points and 9.57 percentage points,respectively.It indicated that the addition of a certain amount ofβ-mercaptoethanol in the mature liquid could improve the developmental ability of oocytes and the quality of fertilized eggs of the 6-week-old Doper sheep.When theβ-mercaptoethanol concentration was within 100μmol/L,the cleavage rate and the blastocyst rate of oocytes were on the rise.The optimal concentration ofβ-mercaptoethanol in the mature liquid still needs further study. | Yangyi MAO Huidi LUO Zhiwu WANG Peng ZHAO Guanwu ZHANG Jun LI Hongyu GUO Li ZHANG Lina XUE Wenqing DANG | 2019 | Agricultural Biotechnology2019，8，4： | 0 |
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