共被期刊论文引用了3次
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| 1 | 儿童哮喘危险因素流行病学研究进展显示文摘哮喘是一种具有明显家庭聚集倾向的多基因遗传性疾病 ,有许多炎症细胞、炎性介质、细胞因子等参与形成气道的慢性炎症反应。儿童的特应性体质低出生体重、非母乳喂养、早期的反复呼吸道感染是发展成持续性哮喘的危险因素 ;室内外环境不良、家庭经济状况、亲子关系不当、胃食管反流、缓解期治疗依从性差与哮喘的发病密切相关。 | 镇永新 杜玉开 | 2003 | 中国社会医学杂志2003，20，2： | 3 |
| 2 | Construction of adeno-associated virus coexpression system for human angiopoietin-1 and VEGF gene显示文摘Background Ischemic disease is one of the leading causes of death in the world. In order to further study gene therapy for ischemic disease, we constructed a recombinant plasmid for co-expression of human angiopoietin-1 and vascular endothelial growth factor 165(VEGF165) gene in adeno-associated virus (AAV) gene delivery system. Methods Human angiopoietin 1 and VEGF165 gene were obtained using PCR. The upstream of angiopoietin 1 contained restriction enzyme site HindⅢ, and the downstream of angiopoietin 1 contained restriction enzyme site BamHⅠ. The upstream of VEGF165 contained restriction enzyme site BglⅡ, and the downstream of VEGF165 contained restriction enzyme site BamHⅠ. Using the multiple cloning sites (MCS) in plasmid pZero++ such as BamHⅠ, BglⅡ, HindⅢ, NotⅠ, XhoⅠ, XbaⅠ, SalⅠ, BspHⅠ, KspⅠ and the corresponding MCS in plasmid pAAV-MCS, angiopoietin 1 and VEGF165 gene were subcloned into pAAV-MCS. Results DNA sequencing revealed that the PCR- amplified angiopoietin 1 and VEGF165 were consistent with NCBI Gene Bank. The recombinant plasmid was identified using PCR and digestion, which proved to be consistent with our hypothesis. In recombinant plasmid, angiopoietin1 and VEGF possessed a CMV promoter and polyA terminator system respectively, thus assuring co-expression of the two genes. Conclusion Successful construction of AAV co-expression system for human angiopoietin 1 and VEGF165 gene will provide the foundation for gene therapy to cure severe ischemic disease. | 陈德杰 谭最 谢友利 刘芳 | 2004 | Chinese Medical Journal2004，，4： | 3 |
| 3 | 针麻下施行喉全切除术的体会显示文摘目的 筛选最佳的以针麻为主 ,辅以少量局麻药的喉全切除术的方法。方法 对 60 4例喉全切除术选用 1 0组不同的体针和耳针的取穴配方 ,并于颈前切口处用 /或不用 0 .5%普鲁卡因 1 0 ml- 2 0 ml的局麻药进行评比。结果 以针麻为主 ,局麻为辅的喉全切除术 ,其优良率可达 82 .47%。结论 采用体针和耳针相结合 ,两法 9穴的针麻方法 ,辅以少量局麻药 ,可有效地进行喉全切除术 ,其成功率可达 1 0 0 %。 | 黄鹤年 邵秋珍 | 2003 | 上海针灸杂志2003，22，7： | 0 |
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